Article
Agriculture, Dairy & Animal Science
Lin Yuan, Hao Wu, Binbin Wang, Cuili Jia, Dongmei Liang, Qing-Ge-Le Caiyin, Jianjun Qiao
Summary: ComX can enhance bacterial competence by modulating global gene expression, leading to improved acid tolerance and nisin yield in Lactococcus lactis. Our study demonstrates a synergistic regulation mechanism between ComX and acid stress on late competence genes expression.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Seyed Hossein Helalat, Carsten Jers, Mandana Bebahani, Hassan Mohabatkar, Ivan Mijakovic
Summary: This study successfully engineered Deinococcus radiodurans R1 to produce pinene using glycerol as a substrate. Through gene expression and metabolic pathway disruption, a strain capable of producing pinene was developed. Additionally, it was found that Deinococcus radiodurans exhibited higher tolerance towards pinene compared to Escherichia coli.
MICROBIAL CELL FACTORIES
(2021)
Article
Agriculture, Dairy & Animal Science
Fernando M. M. Sanca, Iago R. Blanco, Meriellen Dias, Andrea M. Moreno, Simone M. M. K. Martins, Marco A. Stephano, Maria A. Mendes, Carlos M. N. Mendonca, Wellison A. Pereira, Pamela O. S. Azevedo, Martin Gierus, Ricardo P. S. Oliveira
Summary: In this study, the inhibitory effects of antimicrobial peptides produced by Lactococcus lactis L2 on pig pathogens were tested. The peptides showed good bacteriostatic and bactericidal effects, especially against Gram-positive bacteria, and their low molecular weight may be responsible for their antimicrobial properties. The use of probiotics in controlling pig pathogens has potential benefits.
Article
Biotechnology & Applied Microbiology
Valerie Laroute, Nathalie Aubry, Marjorie Audonnet, Muriel Mercier-Bonin, Marie-Line Daveran-Mingot, Muriel Cocaign-Bousquet
Summary: This study combined genotypic and phenotypic approaches to investigate the diversity of GABA production in lactococci. Through comparative genomic analysis and phenotypic analysis, it was found that GABA production varied widely among different strains and species, and the regulation of GAD gene expression was identified as the major contributor to this diversity.
MICROBIAL CELL FACTORIES
(2023)
Article
Biology
Valerie Laroute, Catherine Beaufrand, Pedro Gomes, Sebastien Nouaille, Valerie Tondereau, Marie-Line Daveran-Mingot, Vassilia Theodorou, Helene Eutamene, Muriel Mercier-Bonin, Muriel Cocaign-Bousquet
Summary: This study found that the Lactococcus lactis NCDO2118 strain produces gamma-aminobutyric acid (GABA) in the gastrointestinal tract, which helps alleviate visceral hypersensitivity induced by stress. This discovery opens up new possibilities for using this GRAS bacterium to manage visceral pain and anxiety in patients with irritable bowel syndrome.
Article
Microbiology
Sun-Wook Jeong, Jun-Ho Kim, Ji-Woong Kim, Chae Yeon Kim, Su Young Kim, Yong Jun Choi
Summary: Deinoxanthin, a xanthophyll with superior antioxidant effects compared to other carotenoids, has attracted attention as a potential natural ingredient in food supplements. By genetically engineering and optimizing processes, an engineered strain of D. radiodurans R1 was developed to efficiently produce deinoxanthin, laying the foundation for industrial applications of extremophilic microorganisms.
Article
Biotechnology & Applied Microbiology
Jungmin Lee, Sojeong Heo, Jihoon Choi, Minsoo Kim, Eunji Pyo, Myounghee Lee, Sangick Shin, Jaehwan Lee, Jaehun Sim, Do-Won Jeong
Summary: Comparative genomic analysis showed that L. lactis may be the best species for glutamic acid production among eight LAB species. Strain HY7803 of L. lactis was selected for its high production of glutamic acid, especially when supplemented with citrate. Principal component analysis revealed differences in amino acid profiles among strains, highlighting HY7803's potential as a starter strain for glutamic acid production.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
A. Arias, G. Feijoo, Mt Moreira
Summary: This study investigates the economic feasibility and environmental impacts of biotechnological co-production of nisin and lactic acid from food-associated industrial waste streams. Sugar beet pulp (SBP) is found to be the best carbon source for nisin production, followed by cheese whey (CW). Corn stover (CS) presents challenges related to enzyme consumption and limited nisin production yield.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Applied
Wenting Lan, Rong Zhang, Tengteng Ji, Dur E. Sameen, Saeed Ahmed, Wen Qin, Jianwu Dai, Li He, Yaowen Liu
Summary: The composite film prepared with a CMC:NS ratio of 5:5 showed the best performance, with the film containing 1.5% L. lactis demonstrating good antibacterial activity and release efficiency.
CARBOHYDRATE POLYMERS
(2021)
Article
Multidisciplinary Sciences
Jordan K. Villa, Runhua Han, Chen-Hsun Tsai, Angela Chen, Philip Sweet, Gabriela Franco, Respina Vaezian, Rok Tkavc, Michael J. Daly, Lydia M. Contreras
Summary: This study identified an sRNA called PprS as a post-transcriptional coordinator of ionizing radiation (IR) recovery in the bacterium Deinococcus radiodurans, playing a crucial role in regulating post-radiation recovery. Knockdown of PprS resulted in reduced survival and growth under IR, highlighting its importance in this process. PprS was found to stabilize transcripts involved in various pathways, presenting an additional layer of sRNA-based control in DNA repair pathways associated with bacterial radioresistance.
SCIENTIFIC REPORTS
(2021)
Article
Food Science & Technology
Federica Cardinali, Roberta Foligni, Ilario Ferrocino, Joanna Harasym, Agnieszka Orkusz, Irene Franciosa, Vesna Milanovic, Cristiana Garofalo, Cinzia Mannozzi, Massimo Mozzon, Luca Cocolin, Andrea Osimani, Lucia Aquilanti
Summary: The aim of this study was to characterize the bacterial and fungal communities in Queijo da Beira Baixa PDO cheese samples from Castelo Branco district, Portugal. The study used viable counts and metataxonomic analyses to analyze the communities, as well as conducted physico-chemical and morpho-textural analyses and analysis of volatile organic compounds. Lactococcus lactis was found to be the dominant bacteria in all samples, while various fungal taxa were also detected. Carboxylic acids were the most detected VOCs in the analyzed cheese samples.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemical Research Methods
Jiapeng Wu, Xingfang Tian, Xiaoning Xu, Xinyi Gu, Jian Kong, Tingting Guo
Summary: This study demonstrated the production of lycopene in engineered L. lactis strains by manipulating metabolic pathways and increasing biomass through activation of aerobic respiration. The engineered L. lactis strain showed potential in protecting intestinal epithelial cells against oxidative damage.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zhengming Zhu, Peishan Yang, Jinhua Yang, Juan Zhang
Summary: This study found that overexpressing the metal ATP-binding cassette (ABC) transporters ZitP and ZitQ can significantly improve the acid tolerance of lactic acid bacteria. Transcriptome analysis revealed differential gene expression related to ABC transporters, metal ion transport, and stress response. The overexpression of the ecfA2 gene (energy-coupling factor transporter ATPase) resulted in an extremely high survival rate during acid stress. This research provides a strategy for constructing robust strains that can be used in the food industry.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yuan Gui, Min Lin, Yongliang Yan, Shijie Jiang, Zhengfu Zhou, Jin Wang
Summary: Deinococcus radiodurans, known as the strongest microorganism in the world, has the ability to adjust and thrive in harsh conditions. The study discovered that the dogH gene, encoding a glycoside hydrolase, plays a crucial role in accumulating osmoprotectants, enhancing the osmotic stress tolerance of D. radiodurans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Reyhaneh Papiran, Javad Hamedi
Summary: The application of adaptive laboratory evolution (ALE) technique enhanced the biomass and nisin production yields of Lactococcus lactis, improving its robustness at higher temperatures and aeration rates. Through the evolution process, increased oxidative and acid resistance were observed, along with the emergence of five single-nucleotide polymorphisms in the genome, leading to more industrial desirable features and improved nisin production capability in stressful conditions.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Lin Lin, Tao Li, Shang Dai, Jiangliu Yu, Xiuqin Chen, Liangyan Wang, Yunguang Wang, Yuejin Hua, Bing Tian
ARCHIVES OF MICROBIOLOGY
(2016)
Article
Microbiology
Tao Li, Yulan Weng, Xiaoqiong Ma, Bing Tian, Shang Dai, Ye Jin, Mengjia Liu, Jiulong Li, Jiangliu Yu, Yuejin Hua
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Multidisciplinary Sciences
Shang Dai, Ye Jin, Tao Li, Yulan Weng, Xiaolin Xu, Genlin Zhang, Jiulong Li, Renjiang Pang, Bing Tian, Yuejin Hua
Article
Multidisciplinary Sciences
Jiangliu Yu, Tao Li, Shang Dai, Yulan Weng, Jiulong Li, Qinghao Li, Hong Xu, Yuejin Hua, Bing Tian
SCIENTIFIC REPORTS
(2017)
Article
Microbiology
Shengjie Li, Jianling Cai, Huizhi Lu, Shuyu Mao, Shang Dai, Jing Hu, Liangyan Wang, Xiaoting Hua, Hong Xu, Bing Tian, Ye Zhao, Yuejin Hua
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Nanoscience & Nanotechnology
Yulan Weng, Jiulong Li, Xingcheng Ding, Binqiang Wang, Shang Dai, Yulong Zhou, Renjiang Pang, Ye Zhao, Hong Xu, Bing Tian, Yuejin Hua
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2020)
Article
Biotechnology & Applied Microbiology
Shang Dai, Zhenming Xie, Binqiang Wang, Ning Yu, Jie Zhao, Yulong Zhou, Yuejin Hua, Bing Tian
Summary: Deinococcus radiodurans, an extreme bacterium with unparalleled resistance to oxidative stresses, utilizes a defense strategy involving dynamic cellular PolyP metabolites complexed with free Mn ions to protect against oxidative damage. The regulation of PolyP metabolism in response to oxidative stress highlights its key role in the resistance mechanism of D. radiodurans. This study provides new insights into the functions of PolyP metabolism in organisms and the cellular resistance of radioresistant bacteria.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Environmental Sciences
Shang Dai, Qi Chen, Meng Jiang, Binqiang Wang, Zhenming Xie, Ning Yu, Yulong Zhou, Shan Li, Liangyan Wang, Yuejin Hua, Bing Tian
Summary: The study showed that the extremophile bacterium Deinococcus radiodurans, especially its mutant strain deficient in cell surface-layer, could efficiently prevent the translocation and damages of Cd or Pb in rice by reducing heavy metal accumulation and improving the antioxidant system in rice.
ENVIRONMENTAL POLLUTION
(2021)
Article
Environmental Sciences
Shang Dai, Binqiang Wang, Yue Song, Zhenming Xie, Chao Li, Shan Li, Yan Huang, Meng Jiang
Summary: Astaxanthin gold nanoparticles can efficiently alleviate Cd toxicity in rice plants by suppressing Cd uptake, scavenging ROS, and enhancing the activity of antioxidants. Additionally, Ast-AuNPs regulate the metabolism of free amino acids and increase the level of non-enzymatic antioxidants in rice seedlings.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biotechnology & Applied Microbiology
Shang Dai, Rui Ye, Jianxiang Huang, Binqiang Wang, Zhenming Xie, Xinwen Ou, Ning Yu, Cheng Huang, Yuejin Hua, Ruhong Zhou, Bing Tian
Summary: This study elucidated the molecular mechanism and accumulation of nanoplastics within bacteria, and demonstrated the shielding and internalization effect of membrane lipids on toxic nanoplastics. This finding suggests the potential role of bacteria in plastic bioremediation.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Agronomy
Meng Jiang, Shang Dai, Yun-Chao Zheng, Rui-Qing Li, Yuan-Yuan Tan, Gang Pan, Ian Max Moller, Shi-Yong Song, Jian-Zhong Huang, Qing-Yao Shu
Summary: A mutation in glutamyl-tRNA reductase in rice improves the synthesis of 5-aminolevulinic acid, a precursor of tetrapyrroles, which plays a crucial role in plant growth regulation. The study demonstrates the enzymatic activity of GluRS, GluTR, and GSAT in ALA synthesis in rice and uncovers the conservation of a specific amino acid in GluTR across plant species.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Chemistry, Multidisciplinary
Meng Jiang, Binqiang Wang, Rui Ye, Ning Yu, Zhenming Xie, Yuejin Hua, Ruhong Zhou, Bing Tian, Shang Dai
Summary: The study reveals that nanoplastics can be translocated from the roots to the grains of crops, leading to adverse effects on crop yield and nutritional quality.
Article
Chemistry, Multidisciplinary
Yue Song, Binqiang Wang, Dengying Qiu, Zhenming Xie, Shang Dai, Chao Li, Shouling Xu, Yunchao Zheng, Shan Li, Meng Jiang
Summary: The research reveals that melatonin plays a significant role in mediating molecular, biochemical, and physiological alterations induced by metallic oxide nanoparticles in rice, by promoting tetrapyrrole biosynthesis and enhancing resistance to oxidative stress.
ENVIRONMENTAL SCIENCE-NANO
(2021)
Article
Chemistry, Multidisciplinary
Meng Jiang, Shang Dai, Binqiang Wang, Zhenming Xie, Jiulong Li, Liangyan Wang, Shan Li, Yuanyuan Tan, Bing Tian, Qingyao Shu, Jianzhong Huang
Summary: The study demonstrates that melatonin in the form of gold nanoparticles can alleviate cadmium toxicity in hydroponically grown rice plants by increasing melatonin content, reducing cadmium levels, restoring chlorophyll biosynthesis, and suppressing Cd-induced oxidative stresses through increased antioxidant enzyme activities.
ENVIRONMENTAL SCIENCE-NANO
(2021)
Article
Microbiology
Jingli Dai, Kaixuan Gao, Tao Yao, Huizhi Lu, Congli Zhou, Miao Guo, Shang Dai, Liangyan Wang, Hong Xu, Bing Tian, Yuejin Hua, Ye Zhao
MICROBIOLOGICAL RESEARCH
(2020)
