Article
Multidisciplinary Sciences
Peter Trosan, Joao Victor Cabral, Ingrida Smeringaiova, Pavel Studeny, Katerina Jirsova
Summary: The study demonstrated that IL13 can enhance the clonogenicity and expression of putative stem cell markers of LECs, maintaining their stem cell morphology, but has no effect on the differentiation of corneal epithelial cells. In P2 stage, IL13-treated cultures exhibited higher proliferative capacity.
Article
Oncology
Chandler R. Keller, Yang Hu, Kelsey F. Ruud, Anika E. VanDeen, Steve R. Martinez, Barry T. Kahn, Zhiwu Zhang, Roland K. Chen, Weimin Li
Summary: The study identified the structural and mechanical properties of human normal breast and invasive ductal carcinoma tissue ECM, successfully generating a protein hydrogel using whole protein extracts of human normal breast ECM. This work advances towards full reconstitution of the human breast tissue microenvironment, potentially accelerating individualized biomedical research and therapeutic development.
Article
Engineering, Biomedical
Wang Xi, Jad Saleh, Ayako Yamada, Caterina Tomba, Barbara Mercier, Sebastien Janel, Tien Dang, Matis Soleilhac, Aurelie Djemat, Huiqiong Wu, Beatrice Romagnolo, Frank Lafont, Rene-Marc Mege, Yong Chen, Delphine Delacour
Summary: This study developed hydrogel scaffolds with different elasticities and investigated their influence on the expansion, organization, and differentiation of intestinal epithelial cells. The results showed that cells on soft substrates exhibited improved characteristics resembling in vivo intestinal tissue, which is of significant importance for the design of biomaterials for ex vivo intestinal models.
Article
Biochemistry & Molecular Biology
Robert Y. S. Cheng, Sandra Burkett, Stefan Ambs, Terry Moody, David A. A. Wink, Lisa A. A. Ridnour
Summary: The small signaling molecule nitric oxide (NO) is associated with chronic inflammation and cancer. NO can have both protective and damaging effects on cells depending on its concentration and exposure time. In this study, chronic exposure to NO was found to cause altered phenotypes in breast epithelial cells, including changes in cellular morphology, genomic stability, and RNA and protein expression. These altered phenotypes were associated with malignant characteristics, such as increased expression of cancer stem cell marker CD44 and enhanced motility.
Article
Oncology
Priya Pai, Guannan Wang, Wei Wen Teo, Diana Raez-Rodriguez, Kathleen L. Gabrielson, Balazs Gyorffy, Bradley M. Downs, Akanksha Aggarwal, Saraswati Sukumar
Summary: Loss of HOXA5 promotes aggressiveness in breast cancer by enhancing NF-xB signaling pathway through reducing IxBa stability. High coexpression of HOXA5 and IxBa is associated with better survival outcomes in breast cancer.
Article
Oncology
Yamei Chen, Shihao Zhu, Tianyuan Liu, Shaosen Zhang, Junting Lu, Wenyi Fan, Lin Lin, Tao Xiang, Jie Yang, Xuan Zhao, Yiyi Xi, Yuling Ma, Guoyu Cheng, Dongxin Lin, Chen Wu
Summary: In this study, a comprehensive analysis of 79 multistage esophageal lesions from 29 ESCC patients was conducted using single-cell RNA sequencing and spatial transcriptomics. The results showed a gradual loss of ANXA1 expression in epithelial cells during lesion progression, which was attributed to the suppression of its transcription factor KLF4. It was also found that ANXA1 is a ligand for fibroblasts and loss of ANXA1 leads to uncontrolled transformation of normal fibroblasts into cancer-associated fibroblasts (CAFs), facilitated by TGF-b secreted by malignant epithelial cells. This highlights the importance of ANXA1/FPR2 signaling in the crosstalk between epithelial cells and fibroblasts in promoting ESCC.
Article
Chemistry, Multidisciplinary
Maria Joao Bessa, Fatima Brandao, Paul H. B. Fokkens, Daan L. A. C. Leseman, A. John F. Boere, Flemming R. Cassee, Apostolos Salmatonidis, Mar Viana, Adriana Vulpoi, Simion Simon, Eliseo Monfort, Joao Paulo Teixeira, Sonia Fraga
Summary: Diverse industries have incorporated engineered nanoparticles (ENP) into their production processes, increasing the risk of worker inhalation exposure. In vitro models, particularly using air-liquid interface (ALI) cell cultures, are valuable for studying ENP toxicity. When comparing submerged and ALI conditions for ENP exposure, it was found that ALI cultures were more sensitive to the cytotoxic effects of ENP on human alveolar epithelial-like cells.
Article
Multidisciplinary Sciences
Barnaby J. Ellis, Conor A. Whitley, Asterios Triantafyllou, Philip J. Gunning, Caroline Smith, Steve D. Barrett, Peter Gardner, Richard J. Shaw, Peter Weightman, Janet M. Risk
Summary: This study aimed to predict malignant transformation of OED using FTIR microscopy and machine learning. The results showed that the subsequent transforming status of OED lesions could be predicted with high sensitivity and specificity based on FTIR data. This study supports the clinical potential of FTIR technology in the surveillance of OED.
Article
Biochemistry & Molecular Biology
Roland Abi Nahed, Maya Elkhoury Mikhael, Deborah Reynaud, Constance Collet, Nicolas Lemaitre, Thierry Michy, Pascale Hoffmann, Frederic Sergent, Christel Marquette, Padma Murthi, Tiphaine Raia-Barjat, Nadia Alfaidy, Mohamed Benharouga
Summary: NLRP7 gene is overexpressed in gestational choriocarcinoma (CC) and may contribute to cancer cells' tolerance to the maternal immune system through regulating immune tolerance-related factors and promoting cell proliferation while inhibiting differentiation.
Article
Microbiology
Gino Castillo, Juan Carlos Mora-Diaz, Rahul K. Nelli, Luis G. Gimenez-Lirola
Summary: This study compared the susceptibility and replication efficiency of human coronavirus NL63 (HCoV-NL63) in different cell culture conditions. LLC-MK2 cells showed the highest susceptibility, followed by airway organotypic cultures and primary human respiratory epithelial cells (HRECs). Low susceptibility and no significant replication were observed in HRECs. This susceptibility was associated with the expression levels of the ACE2 receptor protein.
MICROBIOLOGY SPECTRUM
(2022)
Article
Cell Biology
Jessica Elswood, Scott J. Pearson, H. Ross Payne, Rola Barhoumi, Monique Rijnkels, Weston W. Porter
Summary: Through studying the differentiation and metabolism of mammary epithelial cells, it was found that mitophagy occurs selectively during the differentiation process. Inhibiting autophagy impairs cell differentiation, while the PRKN protein is necessary for cell differentiation.
Article
Multidisciplinary Sciences
Bernard C. H. Lee, Philip S. Robinson, Tim H. H. Coorens, Helen H. N. Yan, Sigurgeir Olafsson, Henry Lee-Six, Mathijs A. Sanders, Hoi Cheong Siu, James Hewinson, Sarah S. K. Yue, Wai Yin Tsui, Annie S. Y. Chan, Anthony K. W. Chan, Siu Lun Ho, Peter J. Campbell, Inigo Martincorena, Simon J. A. Buczacki, Siu Tsan Yuen, Suet Yi Leung, Michael R. Stratton
Summary: The study shows that normal tissues in Lynch Syndrome (LS) patients are genomically stable, while ancestor cells of neoplastic tissues undergo multiple cycles of clonal evolution. This highlights important differences in the pathogenesis of LS from other colorectal cancer predisposition syndromes.
NATURE COMMUNICATIONS
(2022)
Article
Oncology
Xun Chen, Liutao Chen, Yuquan Tang, Yi He, Kuangwu Pan, Linyu Yuan, Weihong Xie, Shangwu Chen, Wei Zhao, Dongsheng Yu
Summary: As the most common post-transcriptional RNA modification, m(6)A methylation extensively regulates the structure and function of RNA. This study explored the characteristics of transcriptome-wide m(6)A modification and major m(6)A regulator expression in the precancerous stage using oral precancerous cells as a model. The findings suggest that precancerous cells show changes in m(6)A modification and identify key m(6)A targets and regulators.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Hyesol Lim, Minsoo Koh, Hao Jin, Mijeong Bae, Seung-Yeon Lee, Kyoung Mee Kim, Joohee Jung, Hyun Jeong Kim, So Yeon Park, Hoe Suk Kim, Woo Kyung Moon, Sejin Hwang, Nam Hoon Cho, Aree Moon
Summary: CAFs can induce an invasive phenotype in non-neoplastic breast epithelial cells, with IL-8 and S100A8 playing a crucial role in this process. Targeting IL-8 and S100A8 may be an effective strategy against breast cancer progression.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Abdul Shukkur Ebrahim, Thanzeela Ebrahim, Hussein Kani, Ahmed S. Ibrahim, Thomas W. Carion, Elizabeth A. Berger
Summary: This study uses ECIS technology to assess and optimize corneal epithelial cells in vitro, generating quantifiable measurements that reflect changes in cell behavior. The findings highlight the importance of optimizing cell density and media choice for comprehensive and quantitative evaluation of corneal epithelial cell structure and function.
SCIENTIFIC REPORTS
(2022)
Article
Peripheral Vascular Disease
Hemang Patel, Juan Chen, Mahendra Kavdia
MICROVASCULAR RESEARCH
(2016)
Article
Oncology
Timothy E. Doyle, Rachel E. Factor, Christina L. Ellefson, Kristina M. Sorensen, Brady J. Ambrose, Jeffrey B. Goodrich, Vern P. Hart, Scott C. Jensen, Hemang Patel, Leigh A. Neumayer
Article
Cardiac & Cardiovascular Systems
Hemang Patel, Juan Chen, Kumuda C. Das, Mahendra Kavdia
CARDIOVASCULAR DIABETOLOGY
(2013)
Article
Cell & Tissue Engineering
Hemang Patel, Soonjo Kwon
CELLULAR AND MOLECULAR BIOENGINEERING
(2009)
Article
Engineering, Biomedical
Arthur Lin, Abhimanyu Sabnis, Soujanya Kona, Sivaniaravindapriya Nattama, Hemang Patel, Jing-Fei Dong, Kytai T. Nguyen
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2010)
Article
Environmental Sciences
Hemang J. Patel, Soonjo Kwon
JOURNAL OF EXPOSURE SCIENCE AND ENVIRONMENTAL EPIDEMIOLOGY
(2013)
Article
Toxicology
Rena Baktur, Hemang Patel, Soonjo Kwon
TOXICOLOGY IN VITRO
(2011)
Article
Toxicology
Hemang Patel, Soomi Eo, Soonjo Kwon
TOXICOLOGY LETTERS
(2011)
Article
Biochemical Research Methods
Hemang Patel, Soonjo Kwon
JOURNAL OF BIOLOGICAL ENGINEERING
(2012)
Article
Biotechnology & Applied Microbiology
HJ Patel, SH Su, C Patterson, KT Nguyen
BIOTECHNOLOGY PROGRESS
(2006)
