Article
Cell Biology
Jeeho Kim, Ra Young Park, Younghoon Kee, Sunjoo Jeong, Takbum Ohn
Summary: Serine/arginine-rich splicing factor 3 (SRSF3) is known to regulate gene expression at the splicing level, but it also has a splicing-independent role. This study shows that SRSF3 interacts with eIF4A1 to control the translation of p21 mRNA, providing a new mode of regulation for p21 expression at the translational level.
CELL DEATH & DISEASE
(2022)
Review
Biochemistry & Molecular Biology
Rong Jia, Zhi-Ming Zheng
Summary: This article summarizes the important role of SRSF3 in cellular processes, including regulation of RNA biogenesis and processing, and its involvement in tumorigenesis. It also introduces the gene, mRNA, and protein structure of SRSF3, the regulatory mechanisms of SRSF3 expression, and the characteristics of SRSF3 targets and binding sequences that contribute to its diverse molecular and cellular functions in tumorigenesis and human diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Cell Biology
Yawen Zhang, Mengmeng Wang, Fanyi Meng, Man Yang, Yinshuang Chen, Xuqin Guo, Weiwei Wang, Yifan Zhu, Yundi Guo, Chunlai Feng, Shen Tian, Hongjian Zhang, Huanqiu Li, Jing Sun, Weipeng Wang
Summary: The high expression of SRSF3 in colorectal cancer (CRC) tissues plays a key role in the proliferation and migration of CRC cells. The novel SRSF3 inhibitor, SFI003, effectively suppresses CRC cell growth through the SRSF3/DHCR24/reactive oxygen species (ROS) pathway.
CELL DEATH DISCOVERY
(2022)
Article
Biochemistry & Molecular Biology
Takahito Nakura, Atsufumi Ozoe, Yuka Narita, Masafumi Matsuo, Fumihiko Hakuno, Naoyuki Kataoka, Shin-Ichiro Takahashi
Summary: This study reveals that RbFox2 and SRSF3 proteins mediate exon 11 inclusion in rat hepatoma cells, with the UGCAUG sequence serving as a key regulator for exon 11 inclusion.
Article
Oncology
Chunxia Zhang, Yinshuang Chen, Fuchao Li, Man Yang, Fanyi Meng, Yawen Zhang, Weichang Chen, Weipeng Wang
Summary: The study identified SRSF3 as a splicing factor involved in the regulation of B7-H3 expression, impacting the prognosis of CRC patients. High expression of SRSF3 and B7-H3 correlates with poor patient outcomes, suggesting SRSF3 as a potential target for regulating B7-H3 expression and CRC therapy.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2021)
Article
Oncology
Yinshuang Chen, Man Yang, Fanyi Meng, Yawen Zhang, Mengmeng Wang, Xuqin Guo, Jie Yang, Hongjian Zhang, Haiyang Zhang, Jing Sun, Weipeng Wang
Summary: SRSF3 is involved in the angiogenesis of colorectal cancer (CRC) by regulating the splicing of SRF. Silencing of SRSF3 inhibits angiogenesis and provides a new direction for antiangiogenic therapy in CRC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Anne Vonada, Amita Tiyaboonchai, Sean Nygaard, Jeffrey Posey, Alexander Mack Peters, Shelley R. Winn, Alessio Cantore, Luigi Naldini, Cary O. Harding, Markus Grompe
Summary: The study demonstrates that therapeutic modified hepatocytes can be efficiently selected in preclinical models by using mildly hepatotoxic acetaminophen, leading to significant expansion of transgene-bearing hepatocytes and reaching therapeutic thresholds in genetic liver diseases.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Audrey-Ann Dumont, Lauralyne Dumont, Delong Zhou, Hugo Giguere, Chantal Pileggi, Mary-Ellen Harper, Denis P. Blondin, Michelle S. Scott, Mannix Auger-Messier
Summary: The study demonstrates the importance of Srsf3 in maintaining mitochondrial integrity during cardiac development, with Srsf3 deletion leading to severe heart remodeling and cardiomyocyte enlargement.
Article
Biochemistry & Molecular Biology
Yingying Che, Mixue Bai, Kun Lu, Lin Fu
Summary: The study investigated the expression and function of SRSF3 in cervical cancer and found that SRSF3 promotes proliferation, migration, and invasion of cervical cancer by regulating the variable splicing of DDX5. This study reveals the oncogenic role of SRSF3 in cervical cancer and emphasizes the importance of the SRSF3-DDX5-AKT axis in tumorigenesis.
MOLECULAR CARCINOGENESIS
(2022)
Article
Biotechnology & Applied Microbiology
Hiroki Ura, Sumihito Togi, Yo Niida
Summary: This study compared the advantages and disadvantages of three RNA-Seq library preparation methods and found that TruSeq detected transcripts and splicing events better than the other methods, accurately measuring gene expression levels and splicing events. TeloPrime had higher coverage at the transcription start site, while SMARTer performed better than TeloPrime in terms of the number of detected transcripts and splicing events among the studied full-length double-stranded cDNA methods.
Review
Biology
Miroslava Kretova, Tomas Selicky, Ingrid Cipakova, Lubos Cipak
Summary: Pre-mRNA splicing is a crucial process in eukaryotic cells to generate mature messenger RNAs for protein synthesis. The splicing process involves the removal of non-coding regions (introns) and joining of coding regions (exons) in pre-mRNAs. The spliceosome, composed of various proteins and factors, carries out the specific steps of splicing. Recent studies have revealed that post-translational modifications of splicing factors play significant roles in the regulation of pre-mRNA splicing, including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation.
Article
Biochemistry & Molecular Biology
Yuta Otani, Ken-ichi Fujita, Toshiki Kameyama, Akila Mayeda
Summary: The core members of EJC can recapitulate cancer-specific mRNA re-splicing in normal cells, indicating their critical role in preventing over-splicing of mRNA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Tea Anastasia Ruiz-Luis, Carlos Ortuno-Pineda, Jose Manuel Galindo-Rosales, Odila Saucedo-Cardenas, Jesus Valdes
Summary: ZO-1 alpha+ and ZO-1 alpha- proteins are expressed in different types of tight junctions. The factors involved in the splicing of the alpha exon are unknown. Our study reveals that the SRSF3-hairpin RNA interaction plays a crucial role in the early recognition of 3'ss for alpha exon activation.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Ulrik Kraemer Sundekilde, Caroline Maag Kristensen, Mette Algot Olsen, Henriette Pilegaard, Martin Kroyer Rasmussen
Summary: The circadian rhythm has a significant impact on various physiological processes, including sleep-wake patterns, eating behavior, and hepatic detoxification. This study investigated the role of hepatic peroxisome proliferator-activated receptor gamma coactivator-1 (PGC-1 alpha) in the circadian regulation of cytochrome P450 enzymes (Cyps) in the liver. The results showed that the expression of circadian regulator genes Bmal1 and Clock was influenced by PGC-1 alpha, and the mRNA content of certain Cyps exhibited rhythmic expression. However, no significant circadian regulation was observed at the protein level. Principal component analysis further demonstrated the distinct differences between the control and PGC-1a LKO mice. These findings provide valuable insights into the molecular mechanisms underlying circadian regulation of Cyps and hepatic detoxification.
Review
Biochemistry & Molecular Biology
Maxime Blijlevens, Jing Li, Victor W. van Beusechem
Summary: Dysregulation of mRNA processing, particularly mRNA splicing, is a hallmark of cancer, providing opportunities for new targeted treatment approaches. The spliceosome, a dynamic complex responsible for precursor mRNA splicing, is part of the supraspliceosome coordinating all co-transcriptional mRNA processing activities. Targeting the spliceosome may offer potential for new therapeutic strategies against lung cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)