Journal
JOURNAL OF SYNCHROTRON RADIATION
Volume 19, Issue -, Pages 257-263Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S0909049512001380
Keywords
nuclear resonant vibrational spectroscopy; real sample temperature(s); cryogenic adhesive; heat transfer; X-ray radiation damage
Categories
Funding
- NIH [GM-65440, EB-001962]
- DOE Office of Biological and Environmental Research
- JASRI
- JST (CREST)
- [ESRF/ID18]
- [APS/03ID]
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There are several practical and intertangled issues which make the experiments of nuclear resonant vibrational spectroscopy (NRVS) on biological samples difficult to perform. The sample temperature is one of the most important issues. In NRVS the real sample temperatures can be very different from the readings on the temperature sensors. In this study the following have been performed: (i) citing and analyzing various existing NRVS data to assess the real sample temperatures during the NRVS measurements and to understand their trends with the samples' loading conditions; (ii) designing several NRVS measurements with (Et4N)[FeCl4] to verify these trends; and (iii) proposing a new sample-loading procedure to achieve significantly lower real sample temperatures and to balance among the intertangled experimental issues in biological NRVS measurements.
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