Article
Chemistry, Physical
J. Royes, V. A. Bjornestad, G. Brun, T. Narayanan, R. Lund, C. Tribet
Summary: Photoswitchable surfactants are used in the design of light-responsive colloids and self-assemblies. This study investigates the intermediate lipid-surfactant assemblies formed during fast isothermal photo-triggered transitions. The experiments reveal that the transient assemblies detected during the photo-triggered kinetics are similar to the stationary states. Additionally, the reorganization speed of surfactant-enriched assemblies is faster than that of lipid-enriched ones.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Review
Chemistry, Physical
Kun Qian, Randall E. Winans, Tao Li
Summary: Understanding the nanostructures of liquid electrolytes is crucial for enhancing electrochemical energy storage capacities, but the solvation structures and molecular dynamics in electrolytes are difficult to probe. Small-angle X-ray scattering (SAXS) offers unique insights into molecular clusters and ion pairs in electrolytes, with excellent time-resolution for studying molecular dynamics. This review focuses on the microscopic understanding of liquid electrolytes by SAXS and highlights the unique capabilities of X-ray scattering for nanostructure characterization.
ADVANCED ENERGY MATERIALS
(2021)
Article
Materials Science, Multidisciplinary
Kakeru Obayashi, Kazutaka Kamitani, Chien-Wei Chu, Ryosuke Kawatoko, Chao-Hung Cheng, Atsushi Takahara, Ken Kojio
Summary: The internal structure of polyurethane adhesive undergoes changes during lap shear deformation, with an increase in the spacing of hard segment domains and the merging of tilt angle with the stretching direction. These findings are of great significance for the practical design of adhesives.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Ezzeldin Metwalli, Klaus Goetz, Tobias Zech, Christian Baer, Isabel Schuldes, Anne Martel, Lionel Porcar, Tobias Unruh
Summary: A customized portable SAXS instrument has been constructed and tested at the D22 SANS instrument at ILL, with optimized lead shielding arrangement and double energy threshold X-ray detector to suppress high-energy gamma radiation background. The upgraded SAXS instrument allows for improved signal-to-noise ratio data and combined SAXS and SANS measurements, enabling simultaneous fitting of SAXS and SANS data from the same sample volume for unambiguous interpretation of scattering data. Advanced in situ/real time investigations are now possible, revealing time-resolved complementary nanoscale structural information.
APPLIED SCIENCES-BASEL
(2021)
Article
Multidisciplinary Sciences
David N. Azulay, Oliver Spaeker, Mnar Ghrayeb, Michaela Wilsch-Brauninger, Ernesto Scoppola, Manfred Burghammer, Ivo Zizak, Luca Bertinetti, Yael Politi, Liraz Chai
Summary: Biofilms are multicellular microbial communities encased in an extracellular matrix, with phenotypic heterogeneity enhancing their survival. The molecular structures, elemental distribution, and possible linkages within biofilms remain unexplored, showcasing the importance of molecular-level heterogeneity in shaping biofilm physiology and development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Ying Li, Qike Li, Richard E. Gillilan, Alireza Abbaspourrad
Summary: This study reveals the reversible modulation of protein-protein and protein-chromophore interactions of Cphycocyanin (C-PC) from Spirulina platensis under hydrostatic pressure. The results show that high pressures can disassemble C-PC into monomers or detached subunits, accompanied by protein unfolding. The pressure-induced changes in structure and color can be precisely manipulated and reversed. This knowledge can be used to design new delivery and optical materials.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Theyencheri Narayanan, Michael Sztucki, Thomas Zinn, Jerome Kieffer, Alejandro Homs-Puron, Jacques Gorini, Pierre Van Vaerenbergh, Peter Boesecke
Summary: This article describes the new technical features and enhanced performance of the ID02 beamline with the Extremely Brilliant Source (EBS) at the ESRF. The beamline enables static and kinetic investigations of a broad range of systems in different size scales and time ranges. Additionally, it allows for multispeckle X-ray photon correlation spectroscopy measurements and relaxation of collimation conditions, resulting in higher flux throughput and lower background. The article highlights the importance of these developments in enabling structural, dynamic, and kinetic investigations of out-of-equilibrium soft matter and biophysical systems.
JOURNAL OF APPLIED CRYSTALLOGRAPHY
(2022)
Article
Engineering, Biomedical
Yi Zhang, Wenkai Zhang, Tim Snow, Ying Ju, Yang Liu, Andrew J. Smith, Sujay Prabakar
Summary: This study investigated the changes in structure and stability of ABPs during crosslinking using GA or EDC at different concentrations through synchrotron small-angle X-ray scattering and thermal and colorimetric methods. The findings reveal that telopeptidyl-helical linkages are preferred at low crosslinker concentrations, while excess crosslinking leads to structural changes and plateauing of stabilities.
ACTA BIOMATERIALIA
(2022)
Article
Biochemistry & Molecular Biology
Chinmayee Dash, Ramyaranjan Das, Deepak K. Sahu, Divyansh Upreti, T. Umasankar Patro, Dillip K. Bisoyi
Summary: The present study aims to establish a correlation between macromolecular parameters obtained from small angle X-ray scattering (SAXS) analysis of pretreated sunn hemp fibers and the dielectric and mechanical properties of their composites. Different pretreatment methods, including dewaxing, alkalization, and microwave irradiation, were used to modify the structure of the fibers, which in turn influenced the macromolecular parameters. The modified fibers showed improved mechanical and electrical properties in the reinforced composites.
Article
Chemistry, Multidisciplinary
Rohan Murty, Mrinal K. Bera, Ian M. Walton, Christina Whetzel, Mark R. Prausnitz, Krista S. Walton
Summary: Encapsulation of biomacromolecules within MOFs can enhance their thermal stability. Through SAXS analysis, we demonstrated that entrapped BSA in ZIF scaffolds exhibited superior thermal stability compared to native BSA, suggesting the inhibition of protein denaturation by MOF confinement.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Pharmacology & Pharmacy
Hani Abdeltawab, Darren Svirskis, Ben J. Boyd, Andrew Hill, Manisha Sharma
Summary: A study developed an injectable system for simultaneous and prolonged release of bupivacaine and ketorolac using poloxamer-based thermoresponsive gelling systems. Formulations exhibited steady and sustained drug release over two weeks, with potential clinical benefits for managing perioperative pain. Small Angle X-ray Scattering experiments showed poloxamers formed gels regardless of matrix composition, while drug loading slightly reduced intermicellar spacing.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Physical
O. R. Santos, D. Reis, A. G. Oliveira-Filho, C. L. P. Oliveira, A. M. Figueiredo Neto
Summary: This study investigates a lyotropic mixture in the calamitic nematic phase and its corresponding calamitic cholesteric phase with the addition of a chiral agent. The structure and local ordering at the nanoscale were analyzed using Small-Angle X-ray Scattering. The results show that the overall shape of the micelles is not modified by the presence of the chiral agent, but the correlation between micelles in the cholesteric phase is increased.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Multidisciplinary
X. Zhang, J. Guo, Y. Liu, X. Hao, X. Ji, Q. Yang
Summary: This paper presents an efficient, unmodified, and clean biological method for preparing high crystallinity nanocellulose from hemp stalks for high-value green applications. The biological method (NCs-B) used enzymes and a small amount of alkali, while the chemical method (NCs-C) used strong acid. Various characterizations were performed to analyze the morphology and distribution of NCs, including small-angle X-ray diffraction, wide-angle diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, contact angle measurements, and Zeta potential analysis. Results showed that NCs-B had higher distribution of unit volume capacity, smaller diameter and length compared to NCs-C. Moreover, NCs-B exhibited higher cellulose utilization rate, crystallinity, hydrophobicity, and cell viability compared to NCs-C.
MATERIALS TODAY CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Kevin Pounot, Giorgio Schiro, Matteo Levantino
Summary: Relevant events during protein function, such as ligand binding/release and interaction with substrates or light, often involve out-of-equilibrium structural dynamics. Time-resolved experimental techniques have been developed to track protein structural changes in real time after a reaction-triggering event. Time-resolved X-ray solution scattering is a promising approach that offers both structural sensitivity and temporal resolution in the femto-to-millisecond time range. This article presents the basic principles of the technique, describes recent relevant results, and discusses computational methods for interpreting time-resolved X-ray solution scattering data.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Mehran Amiri, Nicolas P. Martin, Ching-Lei Feng, Jacob Kreager Lovio, May Nyman
Summary: Polyoxometalates are molecular metal oxides that can be isolated without capping ligands, showing high negative charge. Carbonate can reassemble with highly reactive decaniobate to form new structures with strong interactions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Thomas Skamris, Xinsheng Tian, Matthias Thorolfsson, Hanne Sophie Karkov, Hanne B. Rasmussen, Annette E. Langkilde, Bente Vestergaard
PHARMACEUTICAL RESEARCH
(2016)
Article
Biochemistry & Molecular Biology
Bente Vestergaard
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2016)
Article
Biochemistry & Molecular Biology
Eva S. Lobbens, Leonid Breydo, Thomas Skamris, Bente Vestergaard, Anna K. Jager, Lene Jorgensen, Vladimir Uversky, Marco van de Weert
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2016)
Article
Biochemistry & Molecular Biology
Fatima Herranz-Trillo, Minna Groenning, Andreas van Maarschalkerweerd, Roma Tauler, Bente Vestergaard, Pau Bernado
Article
Chemistry, Multidisciplinary
Carlotta Marasini, Bente Vesterga, Bente Vestergaard
Article
Chemistry, Physical
Valeria Vetri, Federica Piccirilli, Johannes Krausser, Gianpiero Buscarino, Urszula Lapinska, Bente Vestergaard, Alessio Zaccone, Vito Fodera
JOURNAL OF PHYSICAL CHEMISTRY B
(2018)
Article
Chemistry, Multidisciplinary
Saskia Bucciarelli, Soren Roi Midtgaard, Martin Nors Pedersen, Soren Skou, Lise Arleth, Bente Vestergaard
JOURNAL OF APPLIED CRYSTALLOGRAPHY
(2018)
Article
Multidisciplinary Sciences
Thomas Skamris, Carlotta Marasini, Kenneth L. Madsen, Vito Fodera, Bente Vestergaard
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Ersoy Cholak, Saskia Bucciarelli, Katrine Bugge, Nicolai Tidemand Johansen, Bente Vestergaard, Lise Arleth, Birthe B. Kragelund, Annette E. Langkilde
Article
Chemistry, Physical
Saskia Bucciarelli, Edres Sayed Sayedi, Silvio Osella, Bartosz Trzaskowski, Karina Juul Vissing, Bente Vestergaard, Vito Fodera
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2020)
Article
Immunology
Kristian Juul-Madsen, Per Qvist, Kirstine L. Bendtsen, Annette E. Langkilde, Bente Vestergaard, Kenneth A. Howard, Martxel Dehesa-Etxebeste, Soren R. Paludan, Gregers Rom Andersen, Poul Henning Jensen, Daniel E. Otzen, Marina Romero-Ramos, Thomas Vorup-Jensen
JOURNAL OF IMMUNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Ersoy Cholak, Katrine Bugge, Adree Khondker, Kimmie Gauger, Elena Pedraz-Cuesta, Morten Enghave Pedersen, Saskia Bucciarelli, Bente Vestergaard, Stine F. Pedersen, Maikel C. Rheinstadter, Annette Eva Langkilde, Birthe B. Kragelund
Article
Biochemistry & Molecular Biology
Line K. Skaanning, Angelo Santoro, Thomas Skamris, Jacob Hertz Martinsen, Anna Maria D'Ursi, Saskia Bucciarelli, Bente Vestergaard, Katrine Bugge, Annette Eva Langkilde, Birthe B. Kragelund
Article
Biochemistry & Molecular Biology
Amin Sagar, Fatima Herranz-Trillo, Annette Eva Langkilde, Bente Vestergaard, Pau Bernado
Summary: This study presents a chemometric approach for decomposing SAXS data, enabling robust decomposition for biomolecular complexes. The method is applicable to different titration points, relative concentrations, and noise effects, with validation showing unique insights into the assemblies.
Meeting Abstract
Chemistry, Multidisciplinary
Soren Skou, Saskia Bucciarelli, Soren R. Midtgaard, Martin N. Pedersen, Lise Arleth, Bente Vestergaard
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES
(2019)