Article
Chemistry, Physical
Junhyun Choi, Chaeyeon Lim, Euiyoung Seo, Jiwhan Ahn, Mohd Danish Khan
Summary: The effects of polyethylene glycol (PEG) molecular weight on malachite flotation performance and its association with solution ionic strength were investigated in this study. The results showed that PEG with a larger molecular weight and higher ionic strength can improve malachite floatability.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Food Science & Technology
Huanhuan Cui, Qihang Liu, David Julian McClements, Bin Li, Shilin Liu, Yan Li
Summary: The study found that incorporating cinnamaldehyde improved the resistance of whey protein isolate-stabilized emulsions to aggregation under high ionic strengths and gastric conditions, keeping them stable in the presence of high salt levels and simulated gastric fluids.
Article
Engineering, Chemical
Yi-Chen Lin, Hui-Hsin Tseng, David K. Wang
Summary: This study systematically explored the use of polyethylene glycol (PEG) with different molecular weights and concentrations as a pore modifier in protein purification membranes, improving water flux, BSA rejection, and antifouling properties. The results showed that different types of PEG had varying effects on membrane performance and revealed the relationship between porogen and membrane property-performance.
JOURNAL OF MEMBRANE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Ahlem Meziadi, Navid Zuberi, Hendrick W. de Haan, Marc A. Gauthier
Summary: This study reveals that the inefficiency of bioconjugation reactions, such as protein PEGylation, is caused by the excluded volume effect (EVE) between methoxy poly(ethylene glycol) (mPEG) and proteins. The researchers found that this EVE leads to the self-assembly of proteins and reactive mPEG into separate protein-rich and mPEG-rich domains. By adding unreactive low-molecular weight mPEG as a co-solvent, the association between the larger protein and reactive mPEG can be promoted, resulting in improved reaction efficiency.
Article
Engineering, Environmental
Guiying Tian, Huan Li, Bilal Khalid, Zijian Zhao
Summary: Blending Al-doped Li7La3Zr2O12 in PEG-LiClO4 matrix enhances ionic conductivity and cycling reversibility. Garnet oxide fillers enlarge amorphous region and improve electrochemical stability, facilitating rapid Li+ migration. The empirical equation proposed simulates the relationship of fillers' mass and volume ratios in composite electrolyte membrane.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Food Science & Technology
Yi Tu, Xinxia Zhang, Li Wang
Summary: Salt addition (NaCl and CaCl2) improved the stability of emulsions formed by rice bran protein (RBP) by enhancing the protein adsorption and physical stability on the oil-water interface. Emulsions with CaCl2 addition exhibited better storage stability and maintained a stable gel-like structure. The study provided insights into the mechanism of salt treated protein particles and contributed to the application of RBP.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Polymer Science
Gento Ishikawa, Tsubasa Tsuji, Satomi Tagawa, Tetsuo Kondo
Summary: The research revealed that amphiphilic cellulose nanofibrils can preferentially adsorb onto hydrophobic materials and lower their melting points when mixed in aqueous media. Visualization using confocal laser microscopy confirmed this phenomenon, indicating a potential novel fusion between synthetic polymeric materials and biomass nano-objects.
Article
Chemistry, Physical
Qi Han, Stuart J. Brown, Calum J. Drummond, Tamar L. Greaves
Summary: Ionic liquids are widely used as solvents for proteins in various fields such as biotechnology, pharmaceutics, and medicine due to their tunable properties. Recent studies have identified key properties of ILs and IL-water mixtures related to protein performance, suggesting the potential for using ILs to inhibit protein aggregation and control protein behavior. This review aims to inspire further advances in understanding protein behavior and optimizing bioprocesses.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Imran Ozer, Garrett Kelly, Renpeng Gu, Xinghai Li, Nikita Zakharov, Parul Sirohi, Smita K. Nair, Joel H. Collier, Michael S. Hershfield, Angus M. Hucknall, Ashutosh Chilkoti
Summary: Protein therapeutics, especially those with PEG decoration, face challenges of short half-life and immunogenicity. The authors developed a poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) conjugate to improve the stability and immunogenicity of PEGylated proteins. The uricase-POEGMA conjugate demonstrated enhanced pharmacokinetics and reduced immunogenicity compared to traditional PEGylated proteins.
Article
Biochemistry & Molecular Biology
Eliandreina Cruz Barrios, Kyra Penino, Onofrio Annunziata
Summary: Diffusiophoresis of tyloxapol micelles in the presence of MgSO4 has been experimentally characterized, and the effect of salt concentration on micelle size and diffusiophoresis has been investigated. A two-state aggregation model has been developed to describe the observed effect, and the main mechanism causing micelle diffusiophoresis has been determined to be preferential hydration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Chemical
Jose C. Diaz, Jovan Kamcev
Summary: Accurate measurements of membrane ionic conductivity are essential for understanding ion transport, but there is no standardized technique for such measurements. Discrepancies in reported ionic conductivity values for common commercial membranes are common. This study presents a non-invasive method based on electrochemical impedance spectroscopy to address external resistances in thin membranes equilibrated with dilute salt solutions.
JOURNAL OF MEMBRANE SCIENCE
(2021)
Article
Engineering, Biomedical
Kosei Utatsu, Tetsuya Kogo, Toru Taharabaru, Risako Onodera, Keiichi Motoyama, Taishi Higashi
Summary: A new transformable mixing-type material, PEG-NH2-PRX, was developed for reversible PEGylation of protein drugs with high mobility of amino groups. Compared to the control material PEG-NH2-DEX, PEG-NH2-PRX efficiently formed complexes with protein drugs, improving stability of antibodies and prolonging hypoglycemic effects of insulin without loss of bioactivity, suggesting it as a promising material for reversible PEGylation of protein drugs.
MATERIALS TODAY BIO
(2021)
Article
Engineering, Chemical
Yang Chen, Zhihui Qu, Hui Hu, Yuanhong Gao
Summary: This research proposed a series of nonaqueous phase change solvents for CO2 capture, including amino-phenolic dual-functionalized ILs and PEGs, which showed good capture and regenerability performance, and could reduce the regeneration heat load.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Engineering, Chemical
Xiaoping Li, Heqi Yang, Dengwei Jing, Lili Wang, Yonggang Xie, Bo Hui
Summary: This study investigated the influence of the ionic properties of polyacrylamides (PAMs) on their turbulence drag reduction performance. Experimental results showed that the friction factors of PAM solutions with different ionic properties were close to theoretical values and were between Newtonian turbulence and the Virk maximum drag reduction asymptote. The study also found that the ionic properties of polymers played an important role in their drag reduction performance, with zwitterionic PAM performing the best, followed by anionic and nonionic PAMs, while cationic PAM had the least effect on drag reduction. Increasing turbulence intensity and flow time decreased the drag reduction effect, while increasing polymer solution concentration and pH value improved the drag reduction rate.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Polymer Science
Jorge L. Olmedo-Martinez, Michele Pastorio, Elena Gabirondo, Alessandra Lorenzetti, Haritz Sardon, David Mecerreyes, Alejandro J. Muller
Summary: In this study, blends of PEO and PHD were prepared and their interactions and crystallization properties were examined using various techniques. The results showed that the blends were partially miscible in the melt, and their crystallization characteristics varied depending on miscibility and composition. The PHD phase significantly influenced the crystallization process of the PEO phase.
Article
Multidisciplinary Sciences
Yasumitsu Sakamoto, Yoshiyuki Suzuki, Ippei Iizuka, Chika Tateoka, Saori Roppongi, Mayu Fujimoto, Koji Inaka, Hiroaki Tanaka, Mitsugu Yamada, Kazunori Ohta, Hiroaki Gouda, Takamasa Nonaka, Wataru Ogasawara, Nobutada Tanaka
SCIENTIFIC REPORTS
(2015)
Article
Multidisciplinary Sciences
Akihiko Nakamura, Takuya Ishida, Katsuhiro Kusaka, Taro Yamada, Shinya Fushinobu, Ichiro Tanaka, Satoshi Kaneko, Kazunori Ohta, Hiroaki Tanaka, Koji Inaka, Yoshiki Higuchi, Nobuo Niimura, Masahiro Samejima, Kiyohiko Igarashi
Article
Instruments & Instrumentation
Hiroaki Tanaka, Susumu Sasaki, Sachiko Takahashi, Koji Inaka, Yoshio Wada, Mitsugu Yamada, Kazunori Ohta, Hiroshi Miyoshi, Tomoyuki Kobayashi, Shigeki Kamigaichi
JOURNAL OF SYNCHROTRON RADIATION
(2013)
Article
Instruments & Instrumentation
Sachiko Takahashi, Kazunori Ohta, Naoki Furubayashi, Bin Yan, Misako Koga, Yoshio Wada, Mitsugu Yamada, Koji Inaka, Hiroaki Tanaka, Hiroshi Miyoshi, Tomoyuki Kobayashi, Shigeki Kamigaichi
JOURNAL OF SYNCHROTRON RADIATION
(2013)
Article
Multidisciplinary Sciences
Takafumi Itoh, Takao Hibi, Fumiko Suzuki, Ikumi Sugimoto, Akihiro Fujiwara, Koji Inaka, Hiroaki Tanaka, Kazunori Ohta, Yutaka Fujii, Akira Taketo, Hisashi Kimoto
Article
Multidisciplinary Sciences
Yasumitsu Sakamoto, Yoshiyuki Suzuki, Ippei Iizuka, Chika Tateoka, Saori Roppongi, Mayu Fujimoto, Koji Inaka, Hiroaki Tanaka, Mika Masaki, Kazunori Ohta, Hirofumi Okada, Takamasa Nonaka, Yasushi Morikawa, Kazuo T. Nakamura, Wataru Ogasawara, Nobutada Tanaka
SCIENTIFIC REPORTS
(2014)
Article
Instruments & Instrumentation
Yoshihisa Suzuki, Kumi Torii, Koji Inaka, Takahisa Fujiwara, Hiroaki Tanaka, Yasutomo Arai
REVIEW OF SCIENTIFIC INSTRUMENTS
(2019)
Review
Crystallography
Yoshinobu Hashizume, Koji Inaka, Naoki Furubayashi, Masayuki Kamo, Sachiko Takahashi, Hiroaki Tanaka
Article
Biochemical Research Methods
Hirohiko Nakamura, Sachiko Takahashi, Koji Inaka, Hiroaki Tanaka
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2020)
Article
Crystallography
Naoki Tanigawa, Sachiko Takahashi, Bin Yan, Masayuki Kamo, Naoki Furubayashi, Koji Kubota, Koji Inaka, Hiroaki Tanaka
Summary: Neutron diffraction experiments are useful for determining the locations of hydrogen atoms in protein molecules, but larger crystals are needed compared to X-ray diffraction. A unique crystallization device and strategy were introduced to grow large protein crystals, producing high-quality crystals with good reproducibility. The device controls crystal growth, monitors in real time, and adjusts concentrations of key components to optimize crystallization conditions.
Article
Crystallography
Hiroaki Tanaka, Rei Utata, Keiko Tsuganezawa, Sachiko Takahashi, Akiko Tanaka
Summary: This study reports on experimental results and simulation studies of protein crystals using viscous polyethylene glycol (PEG) solvents. Through fluorescence correlation spectroscopy, the diffusion rates of proteins, peptides, and small molecules in viscous PEG solvents were investigated, and equations were developed to predict diffusion coefficients. A numerical model was also created to describe diffusion processes during crystallization.
Review
Physics, Applied
Sachiko Takahashi, Misako Koga, Bin Yan, Naoki Furubayashi, Masayuki Kamo, Koji Inaka, Hiroaki Tanaka
INTERNATIONAL JOURNAL OF MICROGRAVITY SCIENCE AND APPLICATION
(2019)