Article
Biochemical Research Methods
Sergio M. Vilas-Boas, Isabella W. Cordova, Kiki A. Kurnia, Heloisa H. S. Almeida, Priscilla S. Gaschi, Joao A. P. Coutinho, Simao P. Pinho, Olga Ferreira
Summary: Countercurrent and centrifugal partition chromatography are techniques for separating compounds from natural extracts, with the selection of the biphasic solvent system being a key design parameter. COSMO-RS and NRTL-SAC models were used to estimate partition coefficients of phenolic compounds in different solvent systems, with the NRTL-SAC model showing higher reliability.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Tim Hammerschick, Walter Vetter
Summary: Centrifugal partition chromatography (CPC) and countercurrent chromatography (CCC) are two preparative techniques used for the isolation and purification of natural products. This study successfully combined the advantages of both techniques by directly linking CPC and CCC, and demonstrated the effectiveness of the hybrid system.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Alvaro Silva Lima, Bruno Sales de Oliveira, Selesa Vanessa Shabudin, Mafalda Almeida, Mara Guadalupe Freire, Katharina Bica
Summary: This work aimed to integrate the extraction and purification of anthocyanins from grape pomace using ATPS and scaled-up CPC, with protic ionic liquids as the common solvent. Different operational conditions led to varied purification factors, with ATPS and CPC achieving significant purification of anthocyanins. The choice of optimal operational conditions depends on the specific application of anthocyanins.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Food Science & Technology
Gabor Endre, Babett Edit Nagy, Daniel Hercegfalvi, Csenge Kasuba, Csaba Vagvolgyi, Andras Szekeres
Summary: Aflatoxins (AFs) are secondary metabolites that cause diseases in animals and humans. Regulations set concentration limits for AFs in EU food and feed products, requiring pure forms for reference standards. Improvements were made to a liquid-liquid chromatographic method to enhance purification and increase the yield of pure AFs. Through a scale-up process, approximately 2.2 g of AFs could be purified using 8.2 L of solvent in a 250 mL rotor, while 7.8 g of AFs could be prepared using 31 L of solvent in a 1000 mL rotor.
Article
Engineering, Chemical
Mafalda R. Almeida, Filipe Ferreira, Pedro Domingues, Joao A. P. Coutinho, Mara G. Freire
Summary: This study successfully developed a method to purify IgY from egg yolk, combining water-soluble protein fraction and aqueous biphasic systems, followed by centrifugal partition chromatography for purification. The operating conditions of CPC were optimized to obtain IgY with high purity.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Anne Pajot, Sebastien Chollet, Elodie Nicolau, Luc Marchal
Summary: This study aims to optimize the recovery and purity of fucoxanthin from Tisochrysis lutea using the CPC technique. CPC provides fast and room temperature extraction without degradation. By optimizing the solvent composition and operating conditions, nine fractions with 100% purity and 92% recovery yield were obtained.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Engineering, Chemical
Bruna P. Soares, Joao H. P. M. Santos, Margarida Martins, Mafalda R. Almeida, Nathalia Santos, Mara G. Freire, Valeria C. Santos-Ebinuma, Joao A. P. Coutinho, Jorge F. B. Pereira, Sonia P. M. Ventura
Summary: This study investigated the continuous purification of EGFP using ABS, with an initial screening conducted by changing the electrolyte content in the ABS to find the optimal conditions. The results showed a high purification rate (89.93%) and recovery yield (82.3%), indicating the potential of FCPC for the continuous purification of EGFP.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Engineering, Chemical
Ariane A. Oshiro, Alexandre M. S. Jorge, Valeria C. Santos-Ebinuma, Jorge F. B. Pereira
Summary: This study proposes an aqueous two-phase system (ATPS) composed of ethanol and dipotassium hydrogen phosphate for Centrifugal Partition Chromatography (CPC). Compared to polymer-based ATPS, the Ethanol/K2HPO4 system overcomes hydrodynamic drawbacks and demonstrates advantages such as short settling time, high interfacial tension, significant density differences, and low viscosity. CPC experiments show that controlling the operating parameters can achieve stable conditions and successful separation of biomolecules using the ethanol/salt ATPS.
SEPARATION AND PURIFICATION TECHNOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Magdalena Maciejewska-Turska, Lukasz Pecio, Grazyna Zgorka
Summary: Pueraria lobata is a legume plant native to Southeast Asia, widely used in traditional medicine. This study developed an original preparative method to efficiently isolate hydrophilic daidzein C-glycosides, including mirificin, from kudzu roots. The isolated mirificin showed a purity of 63% and exhibited significant anti-biodegenerative effects.
Article
Engineering, Chemical
Apostolis Angelis, Dimitris Michailidis, Lemonia Antoniadi, Panagiotis Stathopoulos, Vasiliki Tsantila, Jean-Marc Nuzillard, Jean-Hugues Renault, Leandros A. Skaltsounis
Summary: This study presents an efficient method for the recovery and purification of phenolic compounds from extra virgin olive oil on a pilot scale, involving liquid-liquid extraction and chromatographic techniques. Special attention was paid to the selection of raw materials and purification of target compounds, resulting in the isolation of new natural products as well as the description of a novel constituent in EVOO.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Ana N. Nunes, Viktoriya Ivasiv, Luis F. Gouveia, Naiara Fernandez, Joana Oliveira, Maria Rosario Bronze, Ana A. Matias
Summary: Replacing synthetic colorants with natural ones is a popular marketing trend, but naturally occurring blue color is scarce. This study developed a more efficient and sustainable alternative method, centrifugal partition chromatography (CPC), for purifying portisins. The CPC process showed a higher efficiency and a lower environmental risk factor compared to reversed phase column chromatography (RP-CC). This research suggests that CPC is an effective and sustainable method for isolating portisins and utilizing blueberry surplus as a natural blue colorant.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemistry & Molecular Biology
Ji Hoon Kim, Je-Seung Jeon, Jung Hoon Kim, Eun Ju Jung, Yun Jung Lee, En Mei Gao, Ahmed Shah Syed, Rak Ho Son, Chul Young Kim
Summary: This study used CPC and HPLC techniques to separate and identify two eudesmane type sesquiterpenes with ARE-inducing activity from the crude extract of Lindera strychnifolia roots.
Article
Biochemical Research Methods
Jean-Baptiste Chagnoleau, Ines L. D. Ryan, Ryan Khedher, Joao AP Coutinho, Thomas Michel, Xavier Fernandez, Nicolas Papaiconomou
Summary: A study was conducted on ternary biphasic systems composed of heptane, 1-butanol or ethyl acetate, and type III or type V deep eutectic solvents based on levulinic acid and choline chloride or thymol. The binodal curves, densities, and phase compositions of the equilibrium phases were determined for seven systems. The partition coefficients of six natural compounds were measured in these systems. Based on the results, a system composed of heptane, 1-butanol, and the deep eutectic solvent choline chloride:levulinic acid at a molar ratio of 1:3 was selected for centrifugal partition chromatography and showed good separation of apigenin, coumarin, beta-ionone, and alpha-tocopherol.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Thomas Michel, Celestine Loyet, Romain Boulho, Magalie Eveno, Gregoire Audo
Summary: Centrifugal partition chromatography (CPC) is a liquid-liquid chromatography technique that separates compounds by continuous partition between two immiscible phases. The main objective of this study was to replace traditional solvents with sustainable alternatives in CPC, leading to the isolation of natural products with high purity.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Fabian Hammerle, Michael Zwerger, Anja Hoeck, Markus Ganzera, Ursula Peintner, Bianka Siewert
Summary: Recently, it has been discovered that certain fungal pigments have significant photoactivity, making them promising candidates for treating microbial infections or malignant diseases. In this study, a separation method for fungal anthraquinones was developed using centrifugal partition chromatography. The method was successfully applied to purify multiple pigments from fungal extracts.
JOURNAL OF SEPARATION SCIENCE
(2022)
