Article
Agronomy
Chun Zhang, Chao-Jie Yu, Qin Yu, Wen-Lei Guo, Tai-Jie Zhang, Xing-Shan Tian
Summary: Glyphosate resistance in Eleusine indica populations from South China is common, with underlying mechanisms including target-site overexpression and gene copy number variation of EPSPS. Individual plants possessing both the TIPS mutation and EPSPS overexpression/copy number variation exhibit evolutionary advantages. The role of (CT)(6) insertion mutation in EPSPS copy number variation warrants further investigation.
PEST MANAGEMENT SCIENCE
(2021)
Article
Agronomy
Jingchao Chen, Zhiling Li, Hailan Cui, Haiyan Yu, Xiangju Li
Summary: This study investigates the resistance levels and potential resistance mechanisms of two goosegrass populations collected from orchards. Results showed that the LL population had a Pro106Leu (P106L) mutation and the SS population had a Pro106Ser (P106S) amino acid substitution. Both populations exhibited higher expression of EPSPS, and the LL population showed higher relative copy number and EPSPS protein content than the SS population. Overall, the study confirms that the simultaneous evolution of mutation and amplification in EPSPS confers moderate glyphosate resistance in goosegrass.
Article
Plant Sciences
Wei Deng, Zhiwen Duan, Yang Li, Cheng Peng, Shuzhong Yuan
Summary: This study investigated the resistance levels and potential resistance mechanisms of Eleusine indica biotypes to glyphosate, a widely used herbicide in non-cultivated land in China. The study found that EPSPS gene overexpression and mutation were common resistance mechanisms. In addition, candidate genes involved in non-target-site resistance were identified.
Article
Plant Sciences
Zhiling Li, Xiangju Li, Hailan Cui, Guodong Zhao, Dan Zhai, Jingchao Chen
Summary: This study investigated the fitness traits of a glyphosate-resistant Eleusine indica population under glyphosate-free conditions, finding that the resistant population exhibited higher dry weight, leaf area, and seed production compared to the susceptible population. Metabolome analysis revealed differences in metabolite levels between the two populations, with some metabolites related to auxin synthesis significantly increased in the resistant population. Further research is needed to confirm the functions of these metabolites.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Jinyao Li, Yu Mei, Lingling Zhang, Lubo Hao, Mingqi Zheng
Summary: This study investigated the resistance levels and mechanisms to glyphosate in Eleusine indica populations collected from different areas in China. The results showed that the majority of E. indica populations had developed resistance to glyphosate due to overexpression of the EPSPS gene, rather than amino acid mutation.
Article
Agriculture, Multidisciplinary
Zhang Chun, Yu Chao-jie, Zhang Tai-jie, Guo Wen-lei, Tian Xing-shan
Summary: This study identified the regulatory network of genes associated with EPSPS overexpression in a glyphosate-resistant E. indica population. Differentially expressed transcript analysis suggested that certain genes were co-expressed with EPSPS in the resistant population. Functional annotation and pathway analysis revealed enrichment of up-regulated genes in chloroplasts and pathways related to shikimate biosynthesis.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Biochemistry & Molecular Biology
Jingbo Li, Zhiqian Zhang, Qi Lei, Bugao Lu, Chenzhong Jin, Xiu Liu, Yanhui Wang, Lianyang Bai
Summary: Long-term reliance on herbicides for weed control in sugarcane fields of Guangxi Zhuang autonomous region has resulted in the evolution of resistance in Eleusine indica. This study examined 96 E. indica lines and found resistance to multiple herbicides, with various resistance mechanisms identified. Diversified weed control tactics should be implemented to prevent further resistance in E. indica.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Agronomy
Denny Kurniadie, Ryan Widianto, Uum Umiyati, Dedi Widayat, Ceppy Nasahi, Ari Budiawan
Summary: This study validates the glyphosate resistance in a widely distributed grass species called Eleusine indica and investigates the mutations in the EPSPS gene of the resistant biotype, as well as the type of herbicides that can control the glyphosate-resistant biotypes. The results show that all biotypes of E. indica are resistant to glyphosate but sensitive to propaquizafop, ametryn, and sulfentrazone herbicides. Some biotypes also have multiple resistances to glyphosate, paraquat, and ammonium glufosinate. Amino acid substitutions were found in the EPSPS gene of the glyphosate-resistant biotypes. Further research is needed to determine the mechanism of resistance to paraquat and ammonium glufosinate in E. indica.
Article
Plant Sciences
Wenlei Guo, Chun Zhang, Siwei Wang, Taijie Zhang, Xingshan Tian
Summary: This study evaluated the influence of temperature on the efficacy of glyphosate on a resistant and susceptible weed biotype. The results showed that glyphosate had improved efficacy at lower temperatures, and the resistant biotype had higher shikimic acid accumulation compared to the susceptible biotype. Additionally, glyphosate absorption and translocation were reduced at lower temperatures.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Yaseen Khalil, Michael B. Ashworth, Heping Han, Yu Qin, Roberto L. Rocha, Brent Pritchard, David Cameron, Hugh J. Beckie
Summary: This study identified the first case of field-evolved glyphosate-resistant capeweed in Western Australia, with the resistant population showing over 11-fold resistance to glyphosate compared to susceptible populations. The resistant capeweed population also showed tolerance to other herbicides, indicating the importance of integrated management strategies to combat herbicide resistance.
PEST MANAGEMENT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Jingchao Chen, Hailan Cui, Zhiling Li, Haiyan Yu, Qiang Hou, Xiangju Li
Summary: Gene mutation is a fundamental mechanism in plant evolution under herbicide selection pressure, with pleiotropic effects on plant growth. We investigated the effects of Pro106Leu (P106L), Pro106Ser (P106S), and Thr102Ile + Pro106Ser (TIPS) mutations on EPSPS functionality and fitness traits in Eleusine indica. Our results revealed changes in EPSPS affinity and resistance levels in the mutants, as well as significant reductions in metabolite levels. The mutated plants showed substantial fitness costs throughout the growth period.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Chun Zhang, Nicholas A. Johnson, Nathan Hall, Xingshan Tian, Qin Yu, Eric L. Patterson
Summary: This study investigates the mechanism of glyphosate resistance in goosegrass, finding that resistance is associated with copy number variation (CNV) of the EPSPS gene, with the variation being driven by sub-telomeric-repeat sequences. This discovery further highlights the importance of subtelomeres in generating genetic variation and provides a unique example of herbicide resistance evolution.
NATURE COMMUNICATIONS
(2023)
Article
Agronomy
Dal-Hoe Koo, Yoonha Ju, Karthik Putta, Rajendran Sathishraj, Nilda Roma-Burgos, Mithila Jugulam, Bernd Friebe, Bikram S. S. Gill
Summary: An Italian ryegrass population from Arkansas, USA developed glyphosate resistance due to EPSPS gene amplification. The physical mapping of amplified copies of EPSPS gene through FISH suggests the presence of these copies in extrachromosomal DNA elements. Further analysis by PFGE and DNA blotting reveals that the extrachromosomal DNA containing EPSPS is approximately 400 kb in size, similar to eccDNA replicon in Amaranthus palmeri.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Charles W. Cahoon, David L. Jordan, Patrick J. Tranel, Alan C. York, Chance Riggins, Richard Seagroves, Matthew Inman, Wesley Everman, Ramon Leon
Summary: Comparing the fitness of herbicide-resistant and herbicide-susceptible weed biotypes is crucial for managing herbicide resistance. Previous research has shown that there is little to no fitness penalty from amplification of the EPSPS gene (a mechanism of glyphosate resistance) in Palmer amaranth. However, in the absence of glyphosate, Palmer amaranth plants with EPSPS amplification are relatively less fit, but this reduced fitness does not result in differences in interference with cotton.
Article
Agriculture, Multidisciplinary
Lixian Wen, Jue Zhong, Ying Cui, Zhenchun Duan, Fei Zhou, ChangYan Li, Weihua Ma, Changxi Yin, Hao Chen, Yongjun Lin
Summary: This study successfully transformed a glyphosate oxidase gene and a glyphosate-tolerant gene into a rice variety using genetic engineering to develop transgenic rice with high glyphosate resistance and low residue levels. This has significant implications for addressing herbicide residue issues in food crops.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)