Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 165, Issue 1, Pages 1-9Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2008.09.006
Keywords
Single particle; Automated data collection; Reconstruction; Cryo-EM
Funding
- NIH [P41RR02250]
- National Center of Research Resources [PN2EY016525]
- Nanomedicine Roadmap Initiative [R90 DK071054, T90 DA022885]
- Robert Welch Foundation
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR002250] Funding Source: NIH RePORTER
- NATIONAL EYE INSTITUTE [PN2EY016525, PN1EY016525] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R90DK071504] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON DRUG ABUSE [T90DA022885] Funding Source: NIH RePORTER
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The JEOL Automated Data Acquisition System (JADAS) is a software system built for the latest generation of the JEOL Transmission Electron Microscopes. It is designed to partially or fully automate image acquisition for ice-embedded single particles under low dose conditions. Its built-in flexibility permits users to customize the order of various imaging operations. In this paper, we describe how JADAS is used to accurately locate and image suitable specimen areas on a grid of ice-embedded particles. We also demonstrate the utility of JADAS by imaging the epsilon 15 bacteriophage with the JEM3200FSC electron cryo-microscope, showing that sufficient images can be collected in a single 8 h session to yield a subnanometer resolution structure which agrees with the previously determined structure. (C) 2008 Elsevier Inc. All rights reserved.
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