Article
Reproductive Biology
Qiqi Li, Xing Du, Lingfang Wang, Kerong Shi, Qifa Li
Summary: This study revealed the regulatory mechanism of TGF-beta 1 on porcine granulosa cell states, identified multiple signaling pathways regulating cell states, and potential therapeutic RNA molecules.
Article
Biochemistry & Molecular Biology
Yangan Huo, Qiqi Li, Liu Yang, Xiaoxue Li, Chen Sun, Yang Liu, Honglin Liu, Zengxiang Pan, Qifa Li, Xing Du
Summary: Increasing evidence shows that lncRNAs are involved in the regulation of follicular development and female fertility, but the mechanism remains largely unknown. This study reveals that the lncRNA SDNOR is a potential regulator in porcine follicular granulosa cells, inhibiting the transcription factor SOX9 and affecting downstream targets. Loss of SDNOR impairs GC morphology, inhibits cell proliferation and viability, reduces E2/P4 index, and suppresses the expression of crucial markers. Additionally, SDNOR elevates the resistance of GCs to oxidative stress and inhibits apoptosis induced by oxidative stress.
Article
Agriculture, Dairy & Animal Science
Jinbi Zhang, Xinxin Qin, Caixia Wang, Yuge Zhang, Yizhe Dou, Shiyong Xu, Jingge Liu, Zengxiang Pan
Summary: This study used high-throughput RNA sequencing to identify differentially expressed genes in granulosa cells from healthy and early atretic ovarian follicles in pigs. GO analysis highlighted hormone metabolism, plasma membrane localization, and transporter activity, while pathway analysis revealed the involvement of steroidogenesis, cell adhesion molecules, and TGF-beta signaling pathways in atresia.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Veterinary Sciences
Fang Chen, Xiaoqing Zhu
Summary: This study investigated the effects of activin A (ACT-A) on porcine ovarian granulosa cells (GCs) apoptosis and the underlying molecular mechanism. The results showed that ACT-A could attenuate apoptosis and enhance estrogen synthesis in GCs. ACT-A also enhanced the expression of estrogen receptor-beta (ER beta) induced by FSH, inhibiting ROS accumulation and apoptosis. These findings suggest a protective role of ACT-A in apoptosis by reducing ROS through ER beta, which can improve follicular function and animal reproductive performance.
VETERINARY SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Leon J. Spicer, John R. Evans, Nicole B. Schreiber
Summary: This study investigated the effects of different hormones on THBS1 gene expression in porcine granulosa cells and the changes of THBS1 mRNA during follicular development. The results showed that FSH, IGF1, and FGF9 inhibited THBS1 mRNA expression, while PGE2 promoted THBS1 mRNA expression. The study also found that the abundance of THBS1 mRNA was higher in large follicles compared to small and medium-sized follicles.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Genetics & Heredity
Yiqing Yang, Yuanyuan Zhou, Xuan Li, Yinlin He, Yinshan Bai, Bingyun Wang, Shengfeng Chen, Canying Liu
Summary: This study comprehensively explored the effects of PG1 on porcine GCs using transcriptome profiling. The results revealed that PG1 regulates several genes associated with GC function and ovarian follicle development. GO and KEGG pathway analyses identified genes related to the immune system, infectious disease, and signaling pathways. Additionally, PPI analysis uncovered DEGs involved in cytokine-cytokine receptor interaction, neuroactive ligand-receptor interaction, and chemokine signaling pathway. This study expanded our understanding of the role of PG1 in immune defense and mammalian ovarian follicular development.
Article
Biochemistry & Molecular Biology
Chenfeng Yuan, Zheng Li, Yun Zhao, Xin Wang, Lu Chen, Zijiao Zhao, Maosheng Cao, Tong Chen, Tariq Iqbal, Boqi Zhang, Wenjing Fan, Yameng Wei, Chunjin Li, Xu Zhou
Summary: This study demonstrated that exosomes play a crucial role in regulating the physiological function of granulosa cells by carrying specific microRNAs, promoting cell proliferation and progesterone synthesis. The MAPK/ERK and WNT/B-CATENIN pathways were found to be involved in these processes.
Article
Genetics & Heredity
Xiuxiu Li, Fali Zhang, Jingya Wang, Yanqin Feng, Shuer Zhang, Lan Li, Jinghe Tan, Wei Shen
Summary: The study found that Cyanidin-3-O-glucoside (C3G) can protect porcine granulosa cells (pGCs) from Zearalenone (ZEN) damage by altering the expression of long non-coding RNAs (lncRNAs). C3G significantly increased cell viability and suppressed ZEN-induced apoptosis in pGCs. This study enriches our understanding of the genetic basis and molecular mechanisms by which C3G mitigates the toxicity of ZEN in pGCs.
Article
Chemistry, Multidisciplinary
Jieun Lee, Wooic Son, Juhyeong Hong, Yoonsung Song, Chul-Su Yang, Yong-Hee Kim
Summary: Sepsis is a systemic inflammatory response syndrome caused by bacterial infection. Current sepsis therapy involving antibiotics has limitations such as side effects and high costs. Therefore, there is a growing need for sepsis treatment free from side effects.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Endocrinology & Metabolism
Yu-Fang Liu, Zi-Yi Liu, Wen-Tao Li, Peng Wang, Xiang-Yu Wang, Ran Di, Xiao-Yun He, Ming-Xing Chu
Summary: The aim of this study was to investigate the molecular mechanisms underlying MLT regulation of autophagy in GCs with different Fec(B) genotypes. The researchers found that autophagy levels were significantly higher in GCs with Fec(B) BB genotype than in those with Fec(B)++ genotype. ATG2B was associated with cell autophagy and highly expressed in GCs with Fec(B) BB genotype. MLT could protect GCs by decreasing ROS levels, especially in GCs with Fec(B) ++ genotype.
JOURNAL OF PINEAL RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Liang Huang, Huan Yuan, Shengjie Shi, Xiangrong Song, Lutong Zhang, Xiaoge Zhou, Lei Gao, Weijun Pang, Gongshe Yang, Guiyan Chu
Summary: The CLOCK protein was found to inhibit the proliferation of porcine ovarian granulosa cells by increasing ASB9 level.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Feng Yang, Qiang Liu, Yanhong Chen, Huizhen Ye, Han Wang, Shenming Zeng
Summary: The study conducted quantitative proteomic and phosphoproteomic analyses on ovarian follicular atresia in porcine, identifying potential biomarkers and pathways involved in the process. The findings suggest novel explanations for the regulation of follicular atresia by analyzing dynamic changes in protein expression and phosphorylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Food Science & Technology
Jinjie Wang, Yankun Li, Huan Yuan, Shengjie Shi, Lutong Zhang, Gongshe Yang, Weijun Pang, Lei Gao, Chuanjiang Cai, Guiyan Chu
Summary: This study investigates the effects of alginic acid (AA) on porcine ovarian granulosa cells (GCs) and oocytes. The results show that AA disrupts estrogen production, induces oxidative stress, apoptosis, and autophagy in GCs. It also impairs oocyte maturation and causes abnormal spindle morphology, chromosome arrangement, and cortical granules distribution. These findings suggest that AA is toxic to sow reproduction.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Biology
Jakub Kulus, Magdalena Kulus, Wieslawa Kranc, Karol Jopek, Maciej Zdun, Malgorzata Jozkowiak, Jedrzej M. Jaskowski, Hanna Piotrowska-Kempisty, Dorota Bukowska, Pawel Antosik, Paul Mozdziak, Bartosz Kempisty
Summary: The study focused on the gene expression profile of porcine granulosa cells during primary in vitro culture, particularly related to ECM formation and intercellular signaling. Most genes were upregulated, providing insight into molecular mechanisms within the ovary which can be used for assisted reproduction techniques and stemness potential of granulosa cells. The research may also serve as preliminary data for further studies on ovarian pathological processes using in vitro granulosa cell cultures.
Article
Biochemistry & Molecular Biology
Xuan Zhang, Dingding Zhang, Hongmin Li, Zhaojun Liu, Yatong Yang, Jiameng Li, Lishiyuan Tang, Jingli Tao, Honglin Liu, Ming Shen
Summary: This study reveals an innovative protective mechanism of melatonin against hypoxia-induced granulosa cell (GC) apoptosis by selectively inhibiting mitochondrial ROS (mtROS) generation. Melatonin activates MTNR1B to promote SOD2 expression, effectively suppressing mtROS-JNK-FOXO1-mediated apoptosis.
Article
Biochemistry & Molecular Biology
Selene Elizabeth Herrera-Vazquez, Octavio Dublan-Garcia, Daniel Arizmendi-Cotero, Leobardo Manuel Gomez-Olivan, Hariz Islas-Flores, Maria Dolores Hernandez-Navarro, Ninfa Ramirez-Duran
Summary: The aim of this study was to evaluate the effect of different concentrations of gelatin, whey protein, and chitosan on the properties of composite edible films and optimize their formulation for food packaging. The results showed that the independent variables significantly affected the water vapor permeability, strength, and solubility of the edible films. The optimized formulation resulted in smooth and transparent films with lower water vapor permeability, maximum strength, and lower solubility.
Review
Plant Sciences
Janette Berenice Gonzalez-Nava, Scarlett Alonso-Carmona, Gauddy Lizeth Manzanares-Leal, Horacio Sandoval-Trujillo, Ninfa Ramirez Duran
Summary: Actinobacteria, particularly Streptomyces, Saccharopolyspora, and Micromonospora, are widely used bacteria in producing industrial compounds due to their biosynthetic capability. Molecular techniques and bioinformatics programs have been developed to aid in the discovery of new natural products in actinobacteria. Rare actinobacteria such as Nocardia and Rhodococcus have been found to produce new industrial compounds through these techniques.
RECORDS OF NATURAL PRODUCTS
(2022)
Article
Immunology
Gauddy Lizeth Manzanares-Leal, Jaime Alberto Coronel-Martinez, Miguel Rodriguez-Morales, Ivan Rangel-Cuevas, Lilia Patricia Bustamante-Montes, Horacio Sandoval-Trujillo, Ninfa Ramirez-Duran
Summary: This study aimed to identify the cultivable aerobic bacterial microbiota in women with cervical cancer and found a dysbiosis of aerobic cervicovaginal microbiome in patients with cervical cancer. This preliminary study provides support for further research on the relationship between cervicovaginal microbiome and cervical cancer.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Environmental Sciences
Yong-Sheng Wang, Sheng-Ji Yang, Muhammad Jamil Ahmad, Zhi-Ming Ding, Ze-Qun Duan, Yang-Wu Chen, Ming Liu, Ai-Xin Liang, Guo-Hua Hua, Li-Jun Huo
Summary: This study investigated the adverse effects of Zinc Pyrithione (ZPT) on mouse oocyte maturation and quality. The results showed that ZPT compromised oocyte maturation by disrupting meiotic cell cycle and causing cytoskeletal defects. In addition, ZPT altered epigenetic modifications and resulted in zinc accumulation, which further impaired the quality of oocytes.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Chunjie Huang, Di Wu, Kejia Zhang, Faheem Ahmed Khan, Nuruliarizki Shinta Pandupuspitasari, Yongsheng Wang, Lijun Huo, Fei Sun
Summary: This study reveals that PFOA impairs female reproductive health by affecting oocyte meiosis, preimplantation development, and ovarian follicle development. It induces oxidative stress and dysbiosis of gut microbiota, leading to alterations in metabolism and subsequently causing fetal growth retardation (FGR). Antioxidant melatonin and medication metformin show potential protective effects against PFOA-induced reproductive impairments. This research expands our understanding of risk factors for human reproductive health and suggests potential therapeutic strategies involving restoration of gut microbiota diversity and intervention of metabolism.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Xinzhe Zhao, Peihao Sun, Mingxiao Liu, Shuanghang Liu, Lijun Huo, Zhiming Ding, Ming Liu, Shuai Wang, Ce Lv, Hanxiao Wu, Liguo Yang, Aixin Liang
Summary: This study investigated the toxic effects of Deoxynivalenol (DON) on bovine mammary alveolar cells (MAC-T) and its underlying mechanism. The results showed that DON inhibited cell proliferation, induced apoptosis, and altered cell morphology. DON also damaged the cell membrane and tight junctions, leading to a decrease in the synthesis of beta-casein and lipid droplets. Additionally, genes related to lipid synthesis and metabolism were found to be affected by DON. This study provides new insights into the toxicological mechanism of DON on MAC-T cells.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Zhi-Ming Ding, Yang-Wu Chen, Muhammad Jamil Ahmad, Yong-Sheng Wang, Sheng-Ji Yang, Ze-Qun Duan, Ming Liu, Cai-Xia Yang, Ai-Xin Liang, Guo-Hua Hua, Li-Jun Huo
Summary: This study investigated the toxicity of Bisphenol F (BPF) on mouse oocyte meiotic maturation and quality. The results showed that BPF could inhibit meiotic progression, disrupt spindle assembly and chromosome alignment, induce oxidative stress and DNA damage, and trigger early apoptosis in mouse oocytes. Furthermore, BPF exposure also led to altered epigenetic modifications. Compared with BPA, BPF had less severe toxic effects on oocyte maturation and spindle structure but induced more DNA damage. In conclusion, BPF is not a safe substitute for BPA.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yong-Sheng Wang, Chao Chen, Muhammad Jamil Ahmad, Fan Chen, Zhi-Ming Ding, Sheng-Ji Yang, Yang-Wu Chen, Ze-Qun Duan, Ming Liu, Ai-Xin Liang, Chang-Jiu He, Guo-Hua Hua, Li-Jun Huo
Summary: This study reveals that WDR62 functions in multiple ways during oocyte meiotic maturation, possibly through its regulation of p-JNK and H3K9 trimethylation.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Reproductive Biology
Cai-Xia Yang, Yu-Wei Yang, Qiao Mou, Lu Chen, Li-Jun Huo, Zhi-Qiang Du
Summary: The study reveals that vitamin C treatment induces global changes in miRNA expression in immature Sertoli cells, which may regulate cell proliferation, energy metabolism, and male reproduction.
Article
Toxicology
Chunjie Huang, Di Wu, Faheem Ahmed Khan, Yongsheng Wang, Junjie Xu, Chunhai Luo, Kejia Zhang, Fei Sun, Lijun Huo
Summary: This study reported the hazardous effects of zinc oxide nanoparticles (ZnO NP) on the development of mouse oocytes and pre-implantation embryos. ZnO NP compromised the meiosis process, caused cellular stress, activated autophagy and apoptosis, disrupted cytoskeleton architecture, and induced DNA damage, leading to developmental arrest and degeneration of oocytes and early embryos. The study suggests that ZnO NP is toxic to oocytes and embryos in mammals, highlighting the need for careful evaluation of human exposure and its impact on reproductive health.
TOXICOLOGY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Yong-Sheng Wang, Li -Dan Zhang, Xv Zhou, Sheng-Ji Yang, Muhammad Jamil Ahmad, Zhi-Ming Ding, Ze-Qun Duan, Ming Liu, Shou-Xin Zhang, Ai-Xin Liang, Guo-Hua Hua, Li -Jun Huo
Summary: This study found that exposure to bromoacetic acid (BAA) can interfere with mouse oocyte maturation by disrupting cytoskeletal dynamics, damaging epigenetic modifications, and inducing accumulation of DNA damage.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Environmental Sciences
Peihao Sun, Xinzhe Zhao, Mingxiao Liu, Hanxiao Wu, Ce Lv, Shuo Zhou, Zhiming Ding, Lijun Huo, Liguo Yang, Aixin Liang
Summary: Exposure to DON during lactation adversely affects the development of mammary glands and reduces the production of beta-casein. RNA-seq analysis shows that DEGs are enriched in inflammatory response and HIF-1 signaling pathway, leading to increased inflammation and myeloper-oxidase activity. DON exposure also impairs blood-milk barrier integrity and hormone balance, resulting in mammary gland injury and decreased beta-casein expression.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
Zhi-Ming Ding, Shang-Ke Wang, Shou-Xin Zhang, Yang-Wu Chen, Yong-Sheng Wang, Sheng-Ji Yang, Yun-Xia Cao, Yi-Liang Miao, Li-Jun Huo
Summary: Triclocarban (TCC) is a harmful chemical that is used globally as a broad-spectrum antibacterial agent and is present in high concentrations in the environment. The exposure to TCC can occur through skin contact, diet, and inhalation, leading to its accumulation in the human body. The impact of TCC on human health, specifically its reproductive toxicity, needs to be evaluated due to the lack of clear understanding about its safety profile. This study examined the effect of TCC on the early embryonic development of mice and identified the mechanisms involved.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
Sheng-Ji Yang, Yong-Sheng Wang, Li-Dan Zhang, Zhi-Ming Ding, Xu Zhou, Ze-Qun Duan, Ming Liu, Ai-Xin Liang, Li-Jun Huo
Summary: This study demonstrated that exposure to propyl gallate (PG) compromised oocyte meiotic maturation in mice by inducing mitochondrial stress, activating apoptosis, inducing DNA damage, and increasing oxidative stress, as well as altering histone methylation level.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Cell Biology
Chen Wang, Hui Sun, John S. S. Davis, Xiaojie Wang, Lijun Huo, Nan Sun, Qianzhi Huang, Xiangmin Lv, Cheng Wang, Chunbo He, Changjiu He, Yang Zhou, Jiyun Wu, Liguo Yang, Guohua Hua
Summary: Female subfertility is a global reproductive issue associated with abnormal ovarian follicular development. This study demonstrates that FHL2 is highly expressed in human and mouse ovaries, especially in granulosa cells (GCs). Knockout of Fhl2 in mice leads to decreased litter size and impaired follicular development. Mechanistic studies reveal that FHL2 regulates Egf and Egfr expression through interacting with transcriptional factors AP-1 or NF-kappa B, and also affects YAP1 expression and activation in GCs. These findings suggest that FHL2 plays a crucial role in follicular development and fertility, providing a potential therapeutic target for subfertility.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Maruf Hasan, Henry Reyer, Michael Oster, Nares Trakooljul, Siriluck Ponsuksilli, Elizabeth Magowan, Dagmar -Christiane Fischer, Klaus Wimmers
Summary: UVB exposure can increase the supply of vitamin D in pig husbandry and does not affect the growth performance of the pigs. After exposure, there are changes in gene expression in the liver, with the pathways for vitamin D synthesis being preferentially initiated.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Zhen Wang, Shu-ying Yi, Yuan-ying Zhang, Yu-di Wang, Han-lin Chen, Yi-jie Guo, Xin-ming Wei, Du-xiao Yang
Summary: Vitamin D can reverse S1P-induced cell death and alleviate EAE symptoms by regulating S1P levels and related signaling pathways.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2024)
Correction
Biochemistry & Molecular Biology
Haixiao Chen, Xing Ji, Xinhua Hu, Lihua Chen, Haiyan Lv, Chengyun Xu, Dun Hong, Ximei Wu
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2024)