Article
Cell & Tissue Engineering
M. Gallorini, R. Di Carlo, S. Pilato, A. Ricci, H. Schweikl, A. Cataldi, A. Fontana, S. Zara
Summary: This study aimed to accelerate the differentiation of human dental pulp stem cells (DPSC) into osteoblastic cells by embedding differentiating factors within liposomes. The results showed that the liposome-encapsulated factors decreased CD90 levels and increased CD73 and CD29 expression, indicating osteoblastic commitment. Additionally, the new liposome formulation enhanced alkaline phosphatase activity and Collagen I secretion, while also reducing H2O2 release and inducing an antioxidant response, suggesting its potential in clinical regenerative applications for tissue engineering.
EUROPEAN CELLS & MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Chatvadee Kornsuthisopon, Sunisa Rochanavibhata, Nunthawan Nowwarote, Kevin A. Tompkins, Waleerat Sukarawan, Thanaphum Osathanon
Summary: The small molecule 6-bromoindirubin-3'-oxime (BIO) effectively modulates the osteogenic differentiation of hDPSCs by activating the Wnt signaling pathway. BIO promotes alkaline phosphatase activity and mineralization, while inhibiting colony formation, cell migration, and adipogenic differentiation. These findings suggest that BIO has potential as a bioactive molecule to enhance dentin repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Christian Morsczeck
Summary: This article summarizes the molecular mechanisms driving osteogenic differentiation of dental follicle cells (DFCs) and discusses other factors that influence this process, providing valuable insights for future research and applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
J. F. Huo, M. L. Zhang, X. X. Wang, D. H. Zou
Summary: The study found that chrysin can promote osteogenic differentiation of DPSCs and enhance DPSC-based bone formation in nude mice and rat models. Proteomics analysis revealed the crucial role of Smad3 in this process.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Cell Biology
Francesco Paduano, Elisabetta Aiello, Paul Roy Cooper, Benedetta Marrelli, Irina Makeeva, Mohammad Islam, Gianrico Spagnuolo, Davide Maged, Danila De Vito, Marco Tatullo
Summary: Dental stem cells have the potential to differentiate into various cell types and can revert back to a stem-like state after osteogenic differentiation, showing a greater potential for further differentiation. The ability to control dedifferentiation of DSCs under physiological conditions could enhance their therapeutic potential in bone tissue engineering.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Wushuang Huang, Qi Huang, Hongwen He, Fang Huang
Summary: This study aimed to investigate the effect and potential mechanism of PER2 on the differentiation of human dental pulp stem cells (hDPSCs). The results showed that PER2 promoted the odontoblastic/osteogenic differentiation of hDPSCs, and this process involved mitochondrial metabolic changes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Ceramics
Ensanya A. Abou Neel, Soumya Sheela, Fatma Mousa AlGhalban, Andi Arjuna, Ifty Ahmed
Summary: This study investigated the morphology, chemistry, thermal characteristics, cytocompatibility, and osteogenic potential of solid and porous phosphate-based glass microspheres for potential use as pulp-capping materials. Through various tests, it was found that both solid and porous GMs showed good compatibility with dental pulp stem cells and could support cell attachment, spreading, and viability. However, cells grown in GMs' extracts showed higher viability but less mineralization compared to direct contact with GMs. Therefore, both types of GMs have potential applicability as pulp-capping materials.
JOURNAL OF NON-CRYSTALLINE SOLIDS
(2023)
Article
Pharmacology & Pharmacy
Elaine A. da Rocha, Marcela M. P. Alvarez, Agatha M. Pelosine, Marcela Rocha O. Carrilho, Ivarne L. S. Tersariol, Fabio D. Nascimento
Summary: The tissue engineering of dental oral tissue is making significant advances, and the use of stem cells shows promise in promoting regenerative dentistry. This study investigated the effects of laser photobiomodulation (PBM) on genes related to inflammation and osteogenesis in Postnatal Human Dental Pulp Stem cells (DPSCs). The results showed that PBM can modulate the expression of several genes in DPSCs, with potential implications in inflammation and tissue mineralization processes.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lina Yu, Liting Zeng, Zeyu Zhang, Guanxiong Zhu, Zidan Xu, Junyi Xia, Jinlong Weng, Jiang Li, Janak Lal Pathak
Summary: This study found that cannabidiol enhances the proliferation, migration, and osteogenic/odontogenic differentiation of dental pulp stem cells (DPSCs). CBD also exhibits anti-inflammatory, chemotactic, anti-microbial, and tissue regenerative potentials. These findings suggest the potential application of CBD in dental pulp and bone regeneration based on DPSCs.
Article
Health Care Sciences & Services
Shankargouda Patil, Rodolfo Reda, Nezar Boreak, Hasan Ahmad Taher, Abdulaziz Abu Melha, Ashraf Albrakati, Thilla Sekar Vinothkumar, Mohammed Mustafa, Ali Robaian, Riyadh Alroomy, Rawabi Jaber Ahmed Kharaf, Taif Sharafuddin Kameli, Ahmed Alkahtani, Hosam Ali Baeshen, Vikrant R. Patil, Luca Testarelli
Summary: The study evaluated the adipogenic and osteogenic potential of human dental pulp stem cells after administration of cordycepin, demonstrating significant effects on the adipogenic and osteogenic differentiation of the cells.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Emel Uzunoglu-Ozyurek, Gizem Onal, Serap Dokmeci
Summary: This study investigated the effect of a combination of MTA and VD3 on the odonto/osteogenic differentiation potential of DPSCs in T1DM patients. The results showed that the differentiation potential of T1DM-derived DPSCs was increased with the VD3 supplement, although it was not as efficient as in the controls.
JOURNAL OF ENDODONTICS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Kangrui Zeng, Weiping Li, Qiongyi Kang, Yutong Li, Qing Cheng, Wenwei Xia
Summary: This study revealed that miR-342-5p inhibits the odonto/osteogenic differentiation of hDPSCs by interfering with Wnt/beta-catenin signaling via targeting Wnt7b.
Article
Biochemistry & Molecular Biology
Anastasia Kotova, Arseniy A. Lobov, Julia A. Dombrovskaya, Valentina Y. Sannikova, Nadezhda A. Ryumina, Polina Klausen, Alexey L. Shavarda, Anna B. Malashicheva, Natella Enukashvily
Summary: Dental stem cells, derived from neural crest stem cells, exhibit differences in functional and biological properties, including neural markers, cell morphology, proliferation rate, and osteogenic differentiation capabilities. Dental pulp stem cells and periodontal ligament stem cells show distinctions in neural markers, nuclear protein expression, proliferation rate, etc.
Article
Dentistry, Oral Surgery & Medicine
Xia Qi, Chunyan Liu, Guohua Li, Haipei Luan, Shujuan Li, Dongru Yang, Zheng Zhou
Summary: In this study, CBD demonstrated bi-phasic effects on HDPC viability, promoting cell migration, enhancing collagen synthesis, and mineralized deposits in HDPCs. The expression of angiogenic and odontogenic genes was increased, and these effects were mainly mediated by CB2 activation of MAPK signaling pathways.
JOURNAL OF DENTISTRY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Wajathip Bulanawichit, Thu Ngoc Yen Nguyen, Patcharee Ritprajak, Nunthawan Nowwarote, Thanaphum Osathanon
Summary: This study investigated the effects of Candida albicans mannans on cytotoxicity, cell proliferation, osteogenic differentiation, and inflammatory-related gene expression in human dental pulp cells. The results showed that the mannans from C. albicans did not affect cytotoxicity or cell proliferation, but significantly attenuated osteogenic differentiation and up-regulated the inflammatory-related gene IL12 expression.
JOURNAL OF ENDODONTICS
(2023)
Article
Biochemistry & Molecular Biology
Abdulhameed Bakreen, Miia Juntunen, Yannick Dunlop, Irene F. Ugidos, Tarja Malm, Susanna Miettinen, Jukka Jolkkonen
Summary: Microglia play a crucial role in post-stroke immunomodulation and brain plasticity, repair, and reorganization. Evaluating adipose tissue-derived mesenchymal stem cells (ADMSCs) and rehabilitation in a rat model of ischemic stroke, it was found that their combined use improved behavioral recovery despite long-term perilesional microglia presence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Vignesh K. Rangasami, Ganesh Nawale, Kenta Asawa, Sandeep Kadekar, Sumanta Samanta, Bo Nilsson, Kristina N. Ekdahl, Susanna Miettinen, Jons Hilborn, Yuji Teramura, Oommen P. Varghese, Oommen P. Oommen
Summary: The study demonstrates that silencing tissue factor (TF) can enhance the therapeutic potential of mesenchymal stem cells (MSCs), showing successful delivery and knockdown of TF gene using a novel nanocarrier. This approach improves the performance of MSCs in a blood model, leading to enhanced differentiation, secretion of anti-inflammatory cytokines, and functional capabilities.
Article
Chemistry, Multidisciplinary
Chiho Moon, George K. Sandor, Edward Chengchuan Ko, Yong-Deok Kim
Summary: The study found that there was no significant difference in postoperative stability between patients treated with clear aligners and traditional fixed appliances for six months after surgery. Patients in the clear aligner group had a shorter preoperative orthodontic treatment period, and fewer premolar teeth and impacted teeth were extracted compared to the traditional fixed appliance group.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Multidisciplinary
Min-A Jeon, George K. Sandor, Edward Chengchuan Ko, Yong-Deok Kim
Summary: This study investigated the changes in mandibular angle, intergonial width, and ramus angulation after mandibular bilateral sagittal split ramus osteotomy (BSSRO) in patients with mandibular prognathism. The results showed that the angle and width changes may be influenced by the surgical technique and time.
APPLIED SCIENCES-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Anastasiia Mykuliak, Alma Yrjanainen, Antti-Juhana Maki, Arjen Gebraad, Ella Lampela, Minna Kaariainen, Toni-Karri Pakarinen, Pasi Kallio, Susanna Miettinen, Hanna Vuorenpaa
Summary: The vasculature is a crucial physiological element in human tissues, and the formation of perfusable vascular networks is essential for tissue modeling. This study co-cultured endothelial cells and stromal cells in a hydrogel to form three-dimensional vascular networks. Comparing mesenchymal stem/stromal cells (MSCs) derived from bone marrow and adipose tissue, it was found that these cells supported the formation of mature and interconnected microvascular networks-on-a-chip. However, the origin of MSCs influenced the phenotypes of the vascular networks, with BMSCs inducing the formation of fully perfusable microvasculature and ASCs resulting in partially perfusable microvascular networks. The study also showed that BMSCs outperformed ASCs in terms of pericytic characteristics and gene expression related to vasculature maturation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell & Tissue Engineering
Elena Herbers, Mimmi Patrikoski, Anita Wagner, Riikka Jokinen, Antti Hassinen, Sini Heinonen, Susanna Miettinen, Hilkka Peltoniemi, Eija Pirinen, Kirsi H. Pietilainen
Summary: Mitochondrial dysfunction in white adipose tissue is associated with obesity. Human adipose-derived stromal/stem cells are a useful tool to study the underlying mechanisms of this dysfunction. Existing protocols for adipogenic differentiation alter the phenotype of white adipocytes and have varying effects on mitochondria. This study aims to find a protocol that can more faithfully recapitulate the white adipocyte phenotype observed in human adipose tissue.
STEM CELLS INTERNATIONAL
(2022)
Article
Cell Biology
Arjen Gebraad, Roope Ohlsbom, Juho J. Miettinen, Promise Emeh, Toni-Karri Pakarinen, Mikko Manninen, Antti Eskelinen, Kirsi Kuismanen, Ana Slipicevic, Fredrik Lehmann, Nina N. Nupponen, Caroline A. Heckman, Susanna Miettinen
Summary: This study investigated the cytotoxicity of Melflufen on BMSCs and its effects on BMSC differentiation and the formation of vascular networks. The results showed that Melflufen has significant cytotoxic effects on BMSCs and affects their differentiation, which could potentially impact therapy outcomes.
Article
Cell Biology
Jenni Karttunen, Mette Heiskanen, Tiina Joki, Anu Hyysalo, Vicente Navarro-Ferrandis, Susanna Miettinen, Susanna Narkilahti, Asla Pitkanen
Summary: In this study, we optimized the ultrafiltration-SEC method for isolating EVs from MSC and neuronal cells. We found that the culture medium had no significant effect on EV yield, while stimulation with KCl reduced EV yield. Furthermore, we emphasized the importance of including NCC as a negative control in all cell culture experiments to reduce batch effects.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Elle Koivunotko, Jasmi Snirvi, Arto Merivaara, Riina Harjumaki, Swarna Rautiainen, Minna Kelloniemi, Kirsi Kuismanen, Susanna Miettinen, Marjo Yliperttula, Raili Koivuniemi
Summary: Adipose-derived mesenchymal stromal cells (ASCs) hold great potential for cellular therapies and can differentiate into endothelial cells (ECs) and other angiogenic cell types. Nanofibrillated cellulose (NFC) hydrogel is a suitable matrix for 3D cell culture of ASCs and can stimulate angiogenesis. This study recommends the use of NFC hydrogel as an animal-free biomaterial scaffold for therapeutic angiogenesis and other cell therapy purposes.
Article
Cell Biology
Laura Hyvari, Sari Vanhatupa, Miina Ojansivu, Minna Kelloniemi, Toni-Karri Pakarinen, Leena Hupa, Susanna Miettinen
Summary: This study explores the regulatory mechanisms of bioactive glass on osteogenic differentiation of human mesenchymal stem cells and reveals the involvement of heat shock protein 27 (HSP27) in this process.
Article
Engineering, Biomedical
Reetta Sartoneva, Inari Lyyra, Maiju Juusela, Vipul Sharma, Heini Huhtala, Jonathan Massera, Minna Kellomaki, Susanna Miettinen
Summary: Surgical treatment of urothelial defects with autologous genital or extragenital tissue grafts is susceptible to complications. Tissue engineering using novel biomaterials such as PLA/PBSu blend could provide new treatment options by supporting growth and differentiation of urothelial cells and smooth muscle cells.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2023)
Article
Polymer Science
Antonia Ressler, Roope Ohlsbom, Andreja Zuzic, Arjen Gebraad, Erkka J. Frankberg, Toni-Karri Pakarinen, Hrvoje Ivankovic, Susanna Miettinen, Marica Ivankovic
Summary: According to the biomimetic bone scaffold design paradigm, a scaffold resembling natural bone tissue is needed for bone tissue regeneration. This study aimed to improve the properties of growth factor-free scaffolds by adding collagen into the chitosan matrix. The composite scaffolds based on chitosan, collagen, and Mg, Se, Sr, Zn-substituted CaPs showed highly porous structure and enhanced stability, with good viability, proliferation, and osteogenic differentiation of human bone marrow-derived mesenchymal stem/stromal cells.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Cell & Tissue Engineering
Alma Kurki, Kaarlo Paakinaho, Markus Hannula, Jari Hyttinen, Susanna Miettinen, Reetta Sartoneva
Summary: Tissue engineering offers a new approach for reconstructing large urethral defects, which currently lack optimal repair methods. Cell-seeded scaffolds, including the novel scPLCL(A2P) scaffold, show promise in preventing urethral stricture and promoting urothelial and stromal tissue regeneration. The scPLCL(A2P) scaffold supports the growth and phenotype maintenance of human urothelial cells (hUC) and human adipose-derived stromal cells (hASC) in both mono- and coculture environments.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)
Article
Materials Science, Biomaterials
Christine Gering, Jenny Parraga, Hanna Vuorenpaa, Lucia Botero, Susanna Miettinen, Minna Kellomaki
Summary: Four versatile modifications of hydrogels were investigated to study the effects on their viscoelastic properties and biological response. The results showed that gelatin-containing hydrogels had better viscoelasticity and induced stronger cell response compared to NaGG-based hydrogels. The study highlighted the importance of both biochemical cues and viscoelasticity in hydrogel materials.
BIOMATERIALS ADVANCES
(2022)
Meeting Abstract
Cell & Tissue Engineering
VigneshKumar Rangasami, Ganesh Nawale, Susanna Miettinen, Bo Nilsson, Yuji Teramura, Jons P. Hilborn, Oommen Varghese, Oommen P. Oommen
TISSUE ENGINEERING PART A
(2022)
Article
Biochemistry & Molecular Biology
Maruf Hasan, Henry Reyer, Michael Oster, Nares Trakooljul, Siriluck Ponsuksilli, Elizabeth Magowan, Dagmar -Christiane Fischer, Klaus Wimmers
Summary: UVB exposure can increase the supply of vitamin D in pig husbandry and does not affect the growth performance of the pigs. After exposure, there are changes in gene expression in the liver, with the pathways for vitamin D synthesis being preferentially initiated.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Zhen Wang, Shu-ying Yi, Yuan-ying Zhang, Yu-di Wang, Han-lin Chen, Yi-jie Guo, Xin-ming Wei, Du-xiao Yang
Summary: Vitamin D can reverse S1P-induced cell death and alleviate EAE symptoms by regulating S1P levels and related signaling pathways.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2024)
Correction
Biochemistry & Molecular Biology
Haixiao Chen, Xing Ji, Xinhua Hu, Lihua Chen, Haiyan Lv, Chengyun Xu, Dun Hong, Ximei Wu
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2024)