Article
Agronomy
Laura Buzon-Duran, Natalia Langa-Lomba, Vicente Gonzalez-Garcia, Jose Casanova-Gascon, Jesus Martin-Gil, Eduardo Perez-Lebena, Pablo Martin-Ramos
Summary: In a study on the efficacy of chitosan oligomers (COS)-amino acid conjugate complexes against three fungal species belonging to the Botryosphaeriaceae family, it was found that these complexes showed promising results in reducing disease severity. Further large-scale field trials may be warranted to explore their potential.
Article
Biodiversity Conservation
Milan Spetik, Dorota Anna Tekielska, Akila Berraf-Tebbal, Jakub Pecenka, Katerina Stuskova, Alla Eddine Mahamedi, Ales Eichmeier
Summary: During a study of grapevine trunk diseases in the Czech Republic, 22 Botryosphaeriaceae-like strains were isolated from four grape cultivars in four vineyards. Four species, including Botryosphaeria dothidea, Diplodia mutila, D. seriata, and Neofusicoccum parvum, were identified for the first time in grapevine in the Czech Republic. Diplodia seriata was the most frequently isolated species, present in all cultivated varieties, while D. mutila was the least frequent, only present in 'Palava'. Pinot Noir was the most tolerant host for Botryosphaeriaceae fungi.
Article
Agronomy
Natalia Langa-Lomba, Laura Buzon-Duran, Pablo Martin-Ramos, Jose Casanova-Gascon, Jesus Martin-Gil, Eva Sanchez-Hernandez, Vicente Gonzalez-Garcia
Summary: This study analyzed the efficacy of chitosan, horsetail, and nettle in controlling grapevine trunk diseases in organic farming. It found a clear synergistic behavior between chitosan and the plant extracts, supporting the possibility of extending their applications in Viticulture.
Article
Horticulture
Mariana Patanita, Andre Albuquerque, Maria Doroteia Campos, Patrick Materatski, Carla M. R. Varanda, Joana A. Ribeiro, Maria do Rosario Felix
Summary: Grapevine trunk diseases (GTDs) are complex and challenging to identify and eradicate. This study investigated the fungal community structure associated with GTDs symptomatic and asymptomatic grapevines. The results showed that symptomatic plants had a lower abundance but higher diversity of GTDs-associated fungi. The composition of fungal endophytic communities was significantly different according to the symptomatology and not affected by the cultivar.
Article
Plant Sciences
Natalia Langa-Lomba, Eva Sanchez-Hernandez, Laura Buzon-Duran, Vicente Gonzalez-Garcia, Jose Casanova-Gascon, Jesus Martin-Gil, Pablo Martin-Ramos
Summary: The chemical composition of Rubia tinctorum root extract was analyzed, revealing over 50 constituents with alizarin-related anthraquinones and phenol compounds as the main phytochemicals. The extract showed strong antifungal activity, which was further enhanced when combined with chitosan oligomers or stevioside. This natural antifungal compound presents a promising alternative for sustainable control methods against grapevine trunk diseases.
Article
Plant Sciences
Olivier Fernandez, Christelle Lemaitre-Guillier, Aurelie Songy, Guillaume Robert-Siegwald, Marc-Henri Lebrun, Philippe Schmitt-Kopplin, Philippe Larignon, Marielle Adrian, Florence Fontaine
Summary: Grapevine trunk diseases pose a global threat to vineyards. These diseases are caused by multiple pathogenic microorganisms and have a long latent phase. Combined abiotic stresses exacerbate disease symptoms, especially with less virulent pathogens.
Article
Plant Sciences
Andras Csoto, Antal Nagy, Nora Laurinyecz, Zora Annamaria Nagy, Csaba Nemeth, Erzsebet Krisztina Nemeth, Anna Csikasz-Krizsics, Nandor Rakonczas, Florence Fontaine, Erzsebet Fekete, Michel Flipphi, Levente Karaffa, Erzsebet Sandor
Summary: Grapevine trunk diseases (GTDs) pose an increasing threat to grape production worldwide. Different grape cultivars show varying degrees of susceptibility to GTDs. A study on Hungarian grape germplasm collections found that Vitis vinifera L. cultivars were more sensitive to GTDs compared to interspecific hybrids containing other Vitis species in their pedigree. The ancestral diversity of grapes confers greater resilience against GTDs.
Article
Microbiology
Damola O. Adejoro, E. Eirian Jones, Hayley J. Ridgway, Dion C. Mundy, Bhanupratap R. Vanga, Simon R. Bulman
Summary: Grapevine trunk diseases (GTDs) pose a significant challenge to viticulture due to a lack of effective control measures. This study investigated the microbiome of grapevines with the disease escape phenotype, which remain healthy under high disease pressure. DNA metabarcoding analysis revealed significant differences in the microbiome of GTD escape vines compared to diseased vines, with higher abundance of Pseudomonas and Hymenobacter bacteria and differential associations of Aureobasidium and Rhodotorula fungi. This is the first report linking the GTD escape phenotype with the grapevine microbiome.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Plant Sciences
Pierluigi Reveglia, Regina Billones-Baaijens, Sandra Savocchia
Summary: Grapevine trunk diseases caused by fungal pathogens pose a serious threat to vineyards worldwide. Current treatment methods are lacking, and the relationship between the pathogen and symptom expression is poorly understood. Research has focused on investigating the pathogenicity and chemical characteristics of phytotoxic metabolites produced by the fungi involved in these diseases. Multidisciplinary studies have also explored the role of these metabolites in pathogenicity, symptom development, and host-pathogen interactions.
Article
Plant Sciences
Noemi Flubacher, Raymonde Baltenweck, Philippe Hugueney, Jochen Fischer, Eckhard Thines, Michael Riemann, Peter Nick, Islam M. Khattab
Summary: In response to global warming, grapevine trunk diseases (GTDs) have become a major concern for viticulture due to the ability of endophytic fungi to become necrotrophic and kill the host plant under stress. A study on Neofusicoccum parvum Bt-67 reveals that the absence of ferulic acid in the fungus leads to the secretion of 4-hydroxyphenylacetic acid (4-HPA), which mimics the effect of auxins on grapevine defense and promotes fungal spread. By examining Vitis suspension cells, researchers have found that 4-HPA inhibits early defense responses and the expression of certain genes involved in defense, suggesting its role in preventing host cell death in the latent phase of N. parvum Bt-67. This study provides important insights into the regulation of GTDs and their colonization process.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Plant Sciences
Pedro Reis, Ana Gaspar, Artur Alves, Florence Fontaine, Cecilia Rego
Summary: The study evaluated the efficacy of different protection strategies for vineyard pruning wounds, finding that the control effect of different protectants on Lasiodiplodia isolates varied depending on grape cultivar and year. LC2017 showed some fungistatic effects, and the combination of two protection strategies may be a promising long-term approach to mitigate the impact of Botryosphaeria dieback.
Article
Plant Sciences
Loukas Kanetis, Demetris Taliadoros, Georgios Makris, Michalis Christoforou
Summary: This study described four species in the Sporocadaceae family, including two species in the genus Seimatosporium and two species in Sporocadus. Pathogenicity trials showed that Sporocadus isolates were more aggressive than Seimatosporium. Additionally, a newly described species and two species reported for the first time in Europe on Vitis vinifera were found, suggesting the potential importance of Sporocadaceae in grapevine trunk disease complex.
Article
Agronomy
Tiziana Nardi, Federica Gaiotti, Diego Tomasi
Summary: This study evaluated the fungi and bacteria communities associated with Corvina vines from two vineyards with different management practices within Valpolicella DOC area in Italy. The results highlighted the potential of vine trunk bark as a source of microbial viable species.
Article
Agronomy
Marketa Kulisova, Maria Vrublevskaya, Petra Lovecka, Blanka Vrchotova, Milena Stranska, Miroslav Kolarik, Irena Kolouchova
Summary: Endophytes are beneficial microorganisms living inside plant tissues, producing various metabolites that can be used in medicinal, agricultural, and industrial applications. This study focused on endophytic fungi from Vitis vinifera, showing their potential as a source of metabolites with a wide range of applications.
Article
Biochemistry & Molecular Biology
Laura Martin, Blanca Garcia-Garcia, Maria del Mar Alguacil
Summary: Esca is a complex and devastating Grapevine Trunk Disease caused by various fungal pathogens. Our study found that Phaeomoniella chlamydospora was the most abundant pathogen, and Aureobasidiaceae could be a potential biocontrol agent for Esca.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Mycology
Sandra Hilario, Liliana Santos, Alan J. L. Phillips, Artur Alves
Summary: Species in the genus Diaporthe are important plant pathogens, but the establishment of robust species concepts for international phytosanitary measures has been challenging. This study demonstrates the high variability of the ITS region, leading to difficulties in species delimitation. Divergent ITS paralogs were found within the genome of D. novem, suggesting possible escape from concerted evolution or duplication events. The study also reports the existence of a putative hybrid in the genus Diaporthe for the first time.
Editorial Material
Plant Sciences
C. P. S. S. Alves, A. M. P. Negreiros, A. P. O. Barros, D. M. S. Souza, S. J. Michereff, R. Sales, K. C. Correia
Article
Agronomy
Eugenio Diogo, Helena Machado, Ana Reis, Carlos Valente, Alan J. L. Phillips, Helena Braganca
Summary: Eucalyptus globulus, the main species of eucalyptus in Portuguese planted forests, experienced an increase in plantations in the second half of the twentieth century due to its use in the paper and pulp industry. Despite initially being free from pests and diseases, the number of insects and pathogens affecting these trees gradually increased. Recent studies identified two Phytophthora species, P. cinnamomi and P. alticola, as the cause of root rot and dieback in E. globulus, with P. alticola being the first report outside of South Africa. The possible factors contributing to the increased occurrence of this disease are discussed.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Francisco Beluzan, Josep Armengol, Paloma Abad-Campos
Summary: This study aimed to evaluate the pathogenicity of 12 soilborne oomycete species on three Prunus hybrid rootstocks, and found significant differences in virulence among different species and isolates.
Article
Agronomy
Catarina Leal, David Gramaje, Florence Fontaine, Nicolas Richet, Patricia Trotel-Aziz, Josep Armengol
Summary: Grapevine trunk diseases (GTDs) cause significant economic losses in wine-producing countries. Researchers conducted nursery experiments using Trichoderma atroviride (Ta) SC1 and Bacillus subtilis (Bs) PTA-271, alone and in combination, to study their biocontrol effect on GTD pathogens. The results showed that these biological treatments reduced the infection rate and fungal isolation of GTD pathogens in grapevine nursery material.
PEST MANAGEMENT SCIENCE
(2023)
Editorial Material
Plant Sciences
R. Lopez-Corrales, S. J. Michereff, R. S. Garcia-Estrada, J. Leon-Felix, K. C. Correia, K. Rabago-Zavala, J. M. Tovar-Pedraza
Article
Agriculture, Multidisciplinary
Moises Bento Tavares, Andreia Mitsa Paiva Negreiros, Allinny Luzia Alves Cavalcante, Sara Hellen Fernandes de Oliveira, Josep Armengol, Rui Sales Junior
Summary: With the discovery of five new species of Monosporascus genus, it has become important to understand the impact of these fungi on cultivated plants. This study aimed to evaluate their pathogenicity in non-cucurbitaceous cultures.
REVISTA CIENCIA AGRONOMICA
(2023)
Review
Microbiology
Allyson Andrade Mendonca, Walter de Paula Pinto-Neto, Giselle Alves da Paixao, Dayane da Silva Santos, Marcos Antonio De Morais Jr, Rafael Barros De Souza
Summary: This review provides an overview of the technological and biological challenges in the production and use of probiotic bacteria for promoting human health. It discusses the challenges in industrial production, from physiology to stress resistance, and emphasizes the importance of maintaining cell viability and vitality in the product. It also highlights the obstacles probiotic cells face within the human body, such as enzymes, antibacterial molecules, and pH changes.
Article
Biochemistry & Molecular Biology
Gilberto Henrique Teles, Mariana Rodrigues Xavier, Jackeline Maria Da Silva, Rafael Barros De Souza, Will de Barros Pita, Marcos Antonio de Morais Jr
Summary: Dekkera bruxellensis is an important yeast in industrial fermentation processes. Acetate, a metabolite found in its cultivations, affects its fermentation capacity. This study investigated the role of acetate metabolism in respiring cells using different nitrogen sources. The results showed that acetate production is crucial for carbon assimilation and provided valuable insights into the oxidative metabolism of this yeast.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Diana S. Pereira, Sandra Hilario, Micael F. M. Goncalves, Alan J. L. Phillips
Summary: Due to cryptic diversification and species complexity, the authors used genealogical concordance criterion and coalescent-based species delimitation models to accurately circumscribe the species boundaries in the Diaporthe genus. The study revealed that previous species descriptions were overestimated, and 52 Diaporthe spp. should be considered as a single species.
Article
Microbiology
Barbara Ribeiro Alves Alencar, Renan Anderson Alves de Freitas, Victor Emanuel Petricio Guimaraes, Rayssa Karla Silva, Carolina Elsztein, Suzyanne Porfirio da Silva, Emmanuel Damilano Dutra, Marcos Antonio de Morais Junior, Rafael Barros de Souza
Summary: In this study, a yeast strain called Meyerozyma caribbica URM 8365 was isolated and tested from soil irrigated with vinasse, which has high mineral content and acidic pH. The strain was able to ferment glucose and use xylose when oxygen was provided. It also showed tolerance to inhibitors and high ethanol production efficiencies in various substrates. A proposed metabolic model allowed for the calculation of carbon allocation for ethanol and/or xylitol production and could be used to design an industrial plant for combined production using different substrates.
Editorial Material
Plant Sciences
E. G. Silva, J. A. Silva, A. P. O. Barros, A. K. V. Santos, S. J. Michereff, Kamila Camara Correia
JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Rayssa Karla Silva, Will de Barros Pita, Marcos Antonio de Morais, Rafael Barros de Souza
Summary: This study investigated the detrimental effect of excessive minerals in industrial substrates on the fermentation performance of Saccharomyces cerevisiae and its effect on the physiology of Dekkera bruxellensis. The highest mineral toxicity was observed with Cu2+, which depended on the level of medium aeration. Copper stimulated respiration and glucose fermentation was hindered by most growth inhibitors, leading to changes in carbon distribution. The negative effect of Cu2+ on yeast fermentation was partially alleviated by Mg2+ and Mn2+, similar to magnesium antagonism observed in S. cerevisiae.
LETTERS IN APPLIED MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Mariana Rodrigues Xavier, Gilberto Henrique Teles, Jennyfer Martins de Carvalho, Leucio Duarte Vieira, Will de Barros Pita, Marcos Antonio de Morais
Summary: This study aimed to investigate the biological defence mechanism used by the yeast Dekkera bruxellensis to tolerate the presence of exogenous H2O2. The combination of glutathione peroxidase and sulfhydryl-containing proteins was found to be the preferred defence mechanism against H2O2, especially under respiratory metabolism. However, the action of this mechanism was suppressed when the cells were metabolizing nitrate.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Review
Microbiology
Diana S. Pereira, Alan J. L. Phillips
Summary: Palm trees are a diverse habitat with intense fungal colonization, making them an important substratum to explore fungal diversity. However, research on palm fungi is still lacking and their taxonomic classification within modern DNA sequence-based classifications needs further study. Palm trees can be considered model plants for studying fungal biodiversity and palm fungi play a key role in biodiversity surveys. It is estimated that there are over 76,000 species of palm fungi worldwide, of which more than 2500 are currently known.
