Article
Plant Sciences
Antonio Calabuig-Serna, Carolina Camacho-Fernandez, Ricardo Mir, Rosa Porcel, Esther Carrera, Isabel Lopez-Diaz, Jose M. Segui-Simarro
Summary: During eggplant microspore embryogenesis, the transition from embryos to calli instead of developing as true embryos is a bottleneck. Studies show that there are no genotype-specific endogenous GR profiles associated with a high embryogenic response. The role of GRs in the development of microspore-derived embryos in eggplant cultures is highlighted, with different GR accumulation patterns influencing the embryogenic response.
PLANT GROWTH REGULATION
(2022)
Review
Plant Sciences
Valeria Cavallaro, Alessandra Pellegrino, Rosario Muleo, Ivano Forgione
Summary: Plant tissue cultures rely on artificial light sources for growth and metabolism. By controlling light quality, intensity, and duration, desired plant characteristics can be achieved and energy can be saved. However, the effects of light on in vitro propagation are conflicting, due to various factors and the lack of a standardized protocol.
Article
Horticulture
Jiaqi Zou, Xiao Zou, Zhichao Gong, Gengxing Song, Jie Ren, Hui Feng
Summary: Curly kale, known for its rich nutrition and health benefits, is highly sought after and considered one of the healthiest vegetables. However, conventional breeding methods for this vegetable take a long time to produce homozygous inbred lines. In this study, the isolated microspore culture (IMC) technique was successfully used to accelerate the breeding cycle of curly kale through efficient cytokinin thidiazuron (TDZ) treatment. The results showed that 0.2 mg/L TDZ had the optimal effect on embryo survival and plant regeneration, significantly shortening the breeding cycle.
Article
Plant Sciences
Eduardo Berenguer, Elena Carneros, Yolanda Perez-Perez, Carmen Gil, Ana Martinez, Pilar S. Testillano
Summary: The use of synthetic small molecule inhibitors of mammalian GSK-3 beta has been shown to improve in vitro embryogenesis efficiency in plants, promoting somatic embryogenesis by activating the BR pathway.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Horticulture
Jialei Ji, Henan Su, Wenxue Cao, Xiaoli Zhang, Hongrun Li, Zhiyuan Fang, Limei Yang, Yangyong Zhang, Mu Zhuang, Yong Wang, Vasiliy Taranov, Honghao Lv
Summary: In this study, QTL mapping was conducted using 109 DH lines derived from a cross between the highly embryogenic genotype 01-88 and the nonembryogenic genotype 02-12. The results showed that a QTL locus controlling cabbage embryogenesis ability was mapped to an interval of 57.4-58.4 Mb on chromosome C03. Furthermore, 12 genes with nonsynonymous mutations were identified in the candidate interval, with the DEFECTIVE KERNEL1 (DEK1) gene being identified as an important candidate gene. This study provides a foundation for cloning genes controlling cabbage microspore embryogenesis and the application of microspore culture technology in cabbage breeding on a large scale.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Anna Mineykina, Ludmila Bondareva, Alexey Soldatenko, Elena Domblides
Summary: The study successfully carried out the entire cycle from inducing embryogenesis to regenerating DH plants of red cabbage, achieving a successful cultivation effect by selecting optimal parameters and factors.
Article
Forestry
Momi Tsuruta, Tsuyoshi E. Maruyama, Saneyoshi Ueno, Takumi Kaneeda, Yoshinari Moriguchi
Summary: This study provides basic information on somatic embryo maturation, germination, and growth performance of pollen-free sugi somatic plantlets derived from different embryogenic cell lines (ECLs). Significant differences in growth rate among ECLs were observed, indicating varying abilities in somatic seedling development.
Article
Plant Sciences
Samira Tajedini, Baratali Fakheri, Mohsen Niazian, Nafiseh Mahdinezhad, Abdelbagi Mukhtar Ali Ghanim, Ali Kazemi Pour, Ivan Ingelbrecht, Mehran E. Shariatpanahi
Summary: Two different androgenesis pathways, shed-microspore culture (SMC) and anther culture (AC), were developed for haploid induction in mutagenized M-1 rice cultivars. The effects of various factors on induction and regeneration phases were evaluated. The SMC method showed better results in terms of the number of regenerated embryos. Chromosome counting and flow cytometry analysis confirmed the presence of haploid plants, providing a valuable foundation for rice breeding research.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Chuanhong Liu, Gengxing Song, Bing Fang, Zhiyong Liu, Jiaqi Zou, Shiyao Dong, Sai Du, Jie Ren, Hui Feng
Summary: Isolated Microspore Culture (IMC) is an efficient method for obtaining homozygous strains, but it is challenging in ornamental kale. Histone acetylation, an important epigenetic mechanism, can promote microspore embryogenesis. The study found that the histone deacetylase inhibitor SAHA can enhance embryogenesis in microspores of cut-flower ornamental kale.
Article
Agronomy
Duong Van Yen, Jing Li
Summary: This research article presents the results of in vitro growth experiments on Stahlianthus thorelii Gagnep, a plant belonging to the family Zingiberaceae, using tubers as explants and specific growth stimulant concentrations in MS media. The study successfully increased the number of shoots and the root/shoot ratio through a series of cultivation techniques, achieving 100% survival rate when acclimatized in soil conditions.
Article
Plant Sciences
Monika Gajecka, Marek Marzec, Beata Chmielewska, Janusz Jelonek, Justyna Zbieszczyk, Iwona Szarejko
Summary: This study investigated the genotype-dependent molecular differences in chloroplast differentiation leading to the formation of green and albino regenerants in microspore culture of barley. The results showed that the failed transition from NEP-dependent to PEP-dependent transcription in plastids was associated with albino regenerants, while a high activity of PEP polymerase and increased expression of certain genes correlated with the production of green regenerants. Understanding these molecular mechanisms may help overcome the issue of albinism in cereal androgenesis.
Article
Multidisciplinary Sciences
Fang Gao, Xi Cao, Caiyun Qin, Shigang Chen, Jufeng Cai, Changbin Sun, Lisheng Kong, Jing Tao
Summary: Embryogenic tissue (ET) is crucial for genetic modification and plant re-generation. This study induced ET from mature zygotic embryos in blue spruce and investigated the effects of different factors on ET proliferation and somatic embryo (SE) maturation. The specific concentrations of 2,4-D and BA during the ET proliferation stage significantly affected both ET growth and the quality of ET for SE maturation. Developing genotype-specific protocols is necessary for efficient production of mature SEs or somatic plants in blue spruce.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Byomkesh Dash, Sudhansu Sekhar Bhuyan, Sandeep Kumar Singh, Manjusha Chandravani, Nibedita Swain, Prachitara Rout, Jawahar Lal Katara, C. Parameswaran, B. N. Devanna, Sanghamitra Samantaray
Summary: Rice is crucial for global food security. This study developed an androgenic protocol for indica rice, contributing to yield improvement.
Article
Multidisciplinary Sciences
Seung Hee Choi, Woo Seok Ahn, Myoung Hui Lee, Da Mon Jin, Areum Lee, Eun Yee Jie, Su Ji Ju, Sung Ju Ahn, Suk Weon Kim
Summary: Whole-plant regeneration through plant tissue culture is a complex process regulated by genetic and environmental factors. Epigenetic regulation has been found to play a crucial role in plant cell differentiation and pluripotency. In this study, the effects of epigenetic regulators on lettuce and tobacco protoplasts were tested, and it was found that these chemicals promoted cell division and callus formation. These findings suggest that these chemicals can be used to enhance plant regeneration and crop improvement.
Article
Agronomy
Yaru Chen, Yan Wang, Liang Xu, Xiaojun Su, Lulu Zhai, Yanling Zhao, Cuiping Zhang, Liwang Liu
Summary: This study investigates the effects of different factors on radish microspore embryogenesis and explores how to establish an efficient IMC system to improve the genetic improvement efficiency of radish.
MOLECULAR BREEDING
(2022)
Article
Biotechnology & Applied Microbiology
Farzad Kianersi, Mohammad Reza Abdollahi, Asghar Mirzaie-asl, Dara Dastan, Faiza Rasheed
PLANT CELL TISSUE AND ORGAN CULTURE
(2020)
Article
Biotechnology & Applied Microbiology
Sara Sohrabi, Mohammad Reza Abdollahi, Asghar Mirzaie-Asl, Hassan Ebrahimi Koulaei, Mohsen Aghaeezadeh, Jose M. Segui-Simarro
Summary: The study found that pretreatments with 0.5 M mannitol and 50μM 5-AzaC, as well as the use of MS basal medium and 60 g/L sucrose, notably improved the efficiency of haploid induction and plant regeneration through ovule culture in sugar beet. This new method could potentially be applicable to other crops as well.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Biotechnology & Applied Microbiology
Esin Ari, Hilal Bedir, Ismail Gokhan Deniz, Ilker Genc, Jose M. Segui-Simarro
Summary: The study focused on exploring the androgenic potential of different wild Turkish V. hispanica genotypes as a basis for identifying bottlenecks in future studies. While almost all genotypes responded to microspore embryogenesis, three main bottlenecks were identified: foam-producing saponins in flowers hindered efficient isolation of microspores, embryos had reduced germination ability, and a dense network of hairy roots prevented the formation of functional root systems.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Review
Biology
Ricardo Mir, Antonio Calabuig-Serna, Jose M. Segui-Simarro
Summary: This review compiles the latest advances in doubled haploid plant production, the application of doubled haploid lines in breeding programs, and future perspectives for alternative technologies for doubled haploid generation in eggplant. Eggplant is globally cultivated for its edible fruit, and breeding programs aim to produce F1 hybrids by crossing highly homozygous pure lines. Doubled haploid individuals can be induced from haploid cells in a single generation, offering a more efficient alternative to traditional self crossing methods.
Article
Plant Sciences
Mohammad Reza Abdollahi, Farzad Kianersi, Sayyed Saeed Moosavi, Dara Dastan, Sepideh Asadi
Summary: This study identified and characterized partial cDNA of two key genes, phenylalanine ammonia-lyase (PAL) and t-anol/isoeugenol synthase (IGS1), involved in the t-anethole biosynthetic pathway in Foeniculum vulgare. The expression patterns of the PAL and IGS1 genes, as well as the accumulation of t-anethole and total phenolics and flavonoids, were evaluated in different fennel genotypes under methyl jasmonate (MeJA) treatments. The findings showed that the PAL and IGS1 genes had differential expression in different fennel genotypes and their expression varied depending on MeJA concentration and time point. The highest expression and content of t-anethole, total phenolics, and total flavonoids were observed in the Shiraz genotype treated with 100 μM MeJA for 24 hours.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Antonio Calabuig-Serna, Carolina Camacho-Fernandez, Ricardo Mir, Rosa Porcel, Esther Carrera, Isabel Lopez-Diaz, Jose M. Segui-Simarro
Summary: During eggplant microspore embryogenesis, the transition from embryos to calli instead of developing as true embryos is a bottleneck. Studies show that there are no genotype-specific endogenous GR profiles associated with a high embryogenic response. The role of GRs in the development of microspore-derived embryos in eggplant cultures is highlighted, with different GR accumulation patterns influencing the embryogenic response.
PLANT GROWTH REGULATION
(2022)
Article
Plant Sciences
Carolina Camacho-Fernandez, Jose M. Segui-Simarro, Ricardo Mir, Kim Boutilier, Patricia Corral-Martinez
Summary: Different types of embryogenic structures exhibit specific cell wall characteristics that determine their developmental fate. Highly embryogenic structures have thin walls rich in specific components, while less embryogenic structures have thick walls and lower values of cell wall components.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Hilal Bedir, Esin Ari, Gulsun Elif Vural, Jose M. Segui-Simarro
Summary: Vaccaria hispanica, an interesting species with attractive agronomic properties and valuable bioactive compounds, has potential applications in various fields. However, there is a lack of studies on in vitro clonal population production. This study investigates the possibilities of regenerating clonal plants from two wild Turkish genotypes through somatic embryogenesis and organogenesis, and identifies suitable explant types and plant growth regulator combinations for inducing different morphogenic processes.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Biotechnology & Applied Microbiology
Roya Asadi, Mohammad Reza Abdollahi, Sayyed Saeed Moosavi, Asghar Mirzaie-Asl
Summary: In this study, the encapsulation of Satureja khuzistanica micro-cuttings using sodium alginate was investigated, resulting in genetically stable plants with high rosmarinic acid content for the first time.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Plant Sciences
Antonio Calabuig-Serna, Ricardo Mir, Paloma Arjona, Jose Maria Segui-Simarro
Summary: In this study, the role of calcium in somatic embryogenesis in carrot was investigated. It was found that high calcium levels and the formation of callose layer are indicators of embryogenesis induction. Ca2+ patterns vary in different embryogenic structures. Modulating intracellular Ca2+ levels can effectively regulate the efficiency of somatic embryogenesis in carrot. Additionally, Ca2+-calmodulin signaling is associated with transcriptional remodeling necessary for embryo progression.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Antonio Calabuig-Serna, Ricardo Mir, Jose M. Segui-Simarro
Summary: In this study, somatic embryogenesis in Arabidopsis was induced using IZEs as explants. The process was characterized at the microscopic level, and specific aspects such as WUS expression, callose deposition, and Ca2+ dynamics were studied. The results showed that somatic embryos are produced from WUS-expressing cells in a finger-like appendix, and Ca2+ levels and callose deposition serve as early markers of the embryogenic regions. The study also found that Ca2+ homeostasis in this system is strictly maintained and cannot be altered to modulate embryo production.
Article
Plant Sciences
Antonio Calabuig-Serna, Ricardo Mir, Rosa Porcel, Jose M. Segui-Simarro
Summary: Brassica napus is a species of high agronomic interest commonly used for studying various processes, including microspore embryogenesis. DH4079 and DH12075 lines, with high and low embryogenic response respectively, are ideal for investigating the mechanisms controlling embryogenesis induction. This study examined the genetic transformation ability of DH4079 plants and compared it with the already studied DH12075 plants. Results showed that DH12075 plants responded to genetic transformation, while DH4079 plants remained recalcitrant, failing to produce any regenerant. The study also tested transient transformation with Agrobacterium rhizogenes, where only DH12075 showed a response. These findings indicate that DH4079 is recalcitrant to Agrobacterium-mediated transformation.
Article
Agronomy
Jose M. Segui-Simarro, Nathanael M. A. Jacquier, Thomas Widiez
Summary: Doubled haploids (DH) have become a powerful tool used by breeding companies to produce pure lines for hybrid seed production. The biotechnological revolution has led to the development of multiple approaches for producing haploids and DHs in various species, offering flexibility and reliability in the process.
DOUBLED HAPLOID TECHNOLOGY, VOL. 1: General Topics, Alliaceae, Cereals
(2021)
Editorial Material
Agronomy
Jose M. Segui-Simarro
DOUBLED HAPLOID TECHNOLOGY, VOL. 3: Emerging Tools, Cucurbits, Trees, Other Species
(2021)
