Journal
PLANT CELL REPORTS
Volume 35, Issue 3, Pages 655-666Publisher
SPRINGER
DOI: 10.1007/s00299-015-1910-x
Keywords
GRAS transcription factor; VaPAT1; Vitis amurensis; Abiotic stress; Gibberellic acid (GA); Stress-related genes
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Funding
- National Science Foundation of China (NSFC) [31130047, 31471857]
- National Key Technology R&D Program of the Ministry of Science and Technology during the Twelfth Five-year Plan Period [2013BAD02B04-1]
- Youth Innovation Promotion Association of CAS [2015281]
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The plant-specific GRAS transcription factor family regulates diverse processes involved in plant growth, development and stress responses. In this study, VaPAT1, a GRAS gene from Vitis amurensis was isolated and functionally characterized. Sequence alignment and phylogenetic analysis showed that VaPAT1 has a high sequence identity to CmsGRAS and OsCIGR1, which belong to PAT1 branch of GRAS family and function in stress resistance. The transcription of VaPAT1 was markedly induced by stress-related phytohormone abscisic acid (ABA) and various abiotic stress treatments such as cold, drought and high salinity, however, it was repressed by exogenous gibberellic acid (GA) application. Overexpression of VaPAT1 increased the cold, drought and high salinity tolerance in transgenic Arabidopsis. When compared with wild type (WT) seedlings, the VaPAT1-overexpression lines accumulated higher levels of proline and soluble sugar under these stress treatments. Moreover, stress-related genes such as AtSIZ1, AtCBF1, AtATR1/MYB34, AtMYC2, AtCOR15A, AtRD29A and AtRD29B showed higher expression levels in VaPAT1 transgenic lines than in WT Arabidopsis under normal growth conditions. Together, our results indicated that VaPAT1 functions as a positive transcriptional regulator involved in grapevine abiotic stress responses.
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