Journal
JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
Volume 69, Issue 2, Pages 453-456Publisher
SPRINGER
DOI: 10.1007/s10971-013-3226-z
Keywords
Sol-gel; Sol-gel preserved enzymes; Oxidizing agent; Ormosil
Categories
Funding
- Ministry of Science
- Eshkol Scholarship
- Infrastructure Project (Tashtiot)
- French-Israeli AFIRST Program
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Chemically reactive sol-gel matrices hold the ability of protecting entrapped enzymes from destruction by external harsh chemicals. We show this concept by exposing alkaline phosphatase (AlP) to a strong oxidizing agent-bromine. In solution, AlP is immediately destroyed by this oxidant. When AlP was entrapped in hybrid silica sol-gel materials carrying double bonds, the reactivity of AlP was preserved after exposure to bromine under conditions which totally destroy it in solution. The matrices studied were vinylated and allylated silicas, and their protectability was compared to n-alkylated silicas and to silica itself. For instance, the reactivity of AlP entrapped in allylated silica after exposure to 25.6 mM bromine solution is 40 times higher than its reactivity when entrapped in pure silica; and in solution the enzyme is totally destroyed at this concentration. Molecular level mechanisms for these observations are proposed.
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