Journal
PLANT BREEDING
Volume 134, Issue 2, Pages 156-163Publisher
WILEY
DOI: 10.1111/pbr.12248
Keywords
Oryza sativa L; chromosome segment substitution lines; molecular marker-assisted selection; high-throughput resequencing
Funding
- 973 program [2013CBA01405]
- Provincial Science Foundation of Jiangsu [BK20130725, BK2011666]
- National Natural Science Foundation [31100863, 31400175]
- Research Fund for the Doctoral Program of Higher Education of China [20113250120001]
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Chromosome segment substitution lines (CSSLs) provide ideal materials for quantitative trait loci (QTLs) mapping and genetic dissection of complex traits. In this study, we developed a set of CSSL population consisting of 175 lines, which were derived between the recipient Guangluai 4' and the donor Nipponbare'. Based on 260 molecular markers, we firstly constructed a physical map of core 97 lines. Then, these 97 lines were further genotyped based on resequencing data, and a resequencing-based physical map was constructed. Compared with the molecular marker-based physical map, the resequencing-based physical map of 97 lines contained 367 substituted segments with 252 newly discovered segments. The total size of the 367 substituted segments was 1,074Mb, which was 2.81 times the size of rice genome. Using the 97 CSSLs as materials, we identified nine QTLs for heading date and three of them were firstly reported. All the QTLs had positive additive effects, ranging from 9.50 to 16.50 days. These CSSLs may greatly help forge a new resource for functional genomics studies and molecular breeding in rice.
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