Article
Chemistry, Applied
Hye-Jin Kang, Ha-Nul Lee, Seong-Jin Hong, Bo-Ram Park, Kashif Ameer, Jeong-Yong Cho, Young-Min Kim
Summary: The study successfully synthesized glucosyl steviosides, improving their stability and showing significant advantages in the experiment. Glucosyl steviosides also exhibited lower glucose generation rates compared to enzymatically modified ST.
Article
Agricultural Engineering
Zhuoyu Zhou, Jie Shen, Qinbing Guo, Yongmei Xia, Xueyi Hu, Xiang Liu, Jing Wu
Summary: This study investigated the enzymatic production pathway of rubusoside from stevioside, the performance of rubusoside in solubilization of vitamins, and the identification of byproducts. Rubusoside significantly enhanced the solubility of Vitamin E and Vitamin D2, showing promise for potential applications beyond just being a sweetener.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Anastasia Zerva, Koar Chorozian, Anastasia S. Kritikou, Nikolaos S. Thomaidis, Evangelos Topakas
Summary: In this study, beta-glucosidase and beta-galactosidase were used to transglycosylate two steviol glycosides, resulting in conversion yields ranging from 33.1% to 34.6% for stevioside and 25.6% to 37.6% for rebaudioside A. By utilizing low-cost industrial byproducts as sugar donors, such as cellulose hydrolyzate and acid whey, the biocatalysts exhibited efficient bioconversion activity, demonstrating potential for a cost-effective process for modifying steviol glycosides.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yehui Tao, Ping Sun, Ruxin Cai, Yan Li, Honghua Jia
Summary: In this study, the fusion of sucrose synthase (SuSy) and stevia glycosyltransferase UGT76G1 was explored in order to achieve complete conversion of stevioside into rebaudioside A. The position of individual transcription units and the fusion order of the genes encoding SuSy and UGT76G1 were found to affect the yield of RebA. Fusion of the C-terminal of SuSy and the N-terminal of UGT76G1 with rigid linkers maintained enzyme activities. This work demonstrates the potential of UGT-SuSy fusion in improving enzymatic glycosylation.
APPLIED SCIENCES-BASEL
(2022)
Article
Food Science & Technology
Jung-Min Park, Jung-Hoon Lee, Jong-Ho Koh, Jin-Man Kim
Summary: This study developed a method to better separate and quantify steviol glycosides in various foods, with validated accuracy and precision.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Plant Sciences
Pin-Hua Lin, Yun-Yang Chao
Summary: This experiment investigated the effects of different water supply conditions on the growth of two djulis varieties, showing that drought stress significantly reduced plant growth and relative water content, increased antioxidant enzyme activities, total soluble sugars, and proline content. The study also revealed differences in drought tolerance mechanisms between Chenopodium formosana and Chenopodium quinoa.
Article
Chemistry, Analytical
Yingjie Luo, Lincai Peng, Zhaoziyuan Yang, Shun Yao, Hang Song
Summary: A method for comprehensive utilization of rebaudioside A, stevioside, and rebaudioside C from natural stevia resources was proposed, with optimized conditions achieving high purity and recovery rates for RA and ST. Recrystallization of ST using lower value mother liquor sugar was found to provide cheaper raw material for the subsequent preparation of high-purity RC. The enriched RC in the liquid phase during the crystallization of ST was more than three times higher than in crude stevia, offering a more cost-effective option for obtaining RC.
Article
Food Science & Technology
Kashif Ameer, Saqib Ameer, Young-Min Kim, Muhammad Nadeem, Mi-Kyung Park, Mian Anjum Murtaza, Muhammad Asif Khan, Muhammad Adnan Nasir, Ghulam Mueen-Ud-Din, Shahid Mahmood, Tusneem Kausar, Muhammad Abubakar
Summary: This study optimized the ultrasound-assisted extraction (UAE) of dietetically important diterpene steviol glycosides (SGs) from Stevia rebaudiana leaves. The results showed that UAE, under the optimized conditions of ethanol concentration, sonication time, and leaf-solvent ratio, achieved the highest extract yield. UAE was found to be superior to conventional maceration extraction (CME) in terms of resource consumption, total extract yield (TEY), and SG yields. The findings support the use of UAE as an ecofriendly and efficient extraction method for industrial scale-up.
Article
Food Science & Technology
Georgia Eleni Tsotsou, Ioanna Potiriadi
Summary: This study establishes a methodology for quality control of stevia extracts in the food industry, using pretreatment and quantification of stevia glycosides and isosteviol. The methodology provides accurate assessment of the components and purity of stevia extracts.
Article
Medicine, Research & Experimental
Linda Tsan, Sandrine Chometton, Anna M. R. Hayes, Molly E. Klug, Yanning Zuo, Shan Sun, Lana Bridi, Rae Lan, Anthony A. Fodor, Emily E. Noble, Xia Yang, Scott E. Kanoski, Lindsey A. Schier
Summary: Habitual early-life consumption of low-calorie sweeteners (LCS) has long-lasting effects on glucose regulation, sugar-motivated behavior, and hippocampal-dependent memory in rats, which may be attributed to changes in nutrient transporter, sweet taste receptor, and central gene pathway expression.
Article
Plant Sciences
Jingjin Yu, Ningli Fan, Tian Hao, Yingjie Bian, Lili Zhuang, Qiuguo Li, Zhimin Yang
Summary: Drought stress is a major environmental factor that causes physiological and biochemical damages in plants. This study investigated the effectiveness of foliar application of ethionine (ETH) in improving drought tolerance and revealed how it alleviates drought stress. The results showed that foliar application of ETH effectively mitigated drought damages by enhancing root viability, antioxidant enzyme activities, soluble sugar contents, and isopentenyladenosine levels in tall fescue.
PLANT GROWTH REGULATION
(2023)
Article
Biochemistry & Molecular Biology
Liangliang Chen, Huayi Pan, Ruxin Cai, Yan Li, Honghua Jia, Kequan Chen, Ming Yan, Pingkai Ouyang
Summary: In this study, new steviol glycosides were enzymatically synthesized from stevioside by using plant enzymes, with rebaudioside E as the main product. The regioselectivity and stereoselectivity of the enzymes were validated, leading to the successful production of a high-intensity sweetener.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Narendren Rengasamy, Rofina Y. Othman, Hang S. Che, Jennifer A. Harikrishna
Summary: The combination of red, blue, and UV-A light proved to be the most effective in improving germination rates and yields for Stevia rebaudiana. While red and blue light combinations were effective for plant growth, supplemental UV-A irradiation significantly delayed flowering and enhanced yields. Overall, the use of red, blue, and UV-A light resulted in the best productivity for Stevia rebaudiana.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Plant Sciences
Yi-Hsuan Lin, Yu-Ka Jin, Zhen-Yuan Chen, Zu-Dong Xiao, Si Shen, Shun-Li Zhou
Summary: This study found that exogenous methylglyoxal (MG) application during the grain-filling stage of maize can enhance drought tolerance and increase yield. MG application delays leaf senescence, improves photosynthesis, and regulates the homeostasis of endogenous MG and sugars.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Hojjat Baroni-nezhad, Mojtaba Karimi, Hamidreza Motaghian, Fatemeh Direkvand-moghadam
Summary: Micronutrients like Copper, Iron, and Zinc do not significantly affect the growth of Stevia, but can markedly increase the content of sweet compounds such as steviol glycosides in the leaves.
JOURNAL OF PLANT NUTRITION
(2021)
Article
Plant Sciences
Mohammadreza Neshat, Alireza Abbasi, Abdulhadi Hosseinzadeh, Mohammad Reza Sarikhani, Davood Dadashi Chavan, Abdolrahman Rasoulnia
Summary: Salinity is a severe abiotic stress that affects canola production. Plant growth-promoting rhizobacteria can alleviate salt stress and improve physiological and biochemical parameters in canola.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2022)
Article
Agronomy
Nasim Khalifeh, Manijeh Sabokdast Nodehi, Alireza Abbasi, Sajjad Sobhanverdi
Summary: This study investigated the effect of salt stress on mung bean genotypes and found that antioxidant activity and proline content were affected. The results suggest that antioxidant enzymes were more active in the tolerant genotype and proline accumulation was increased due to gene regulation. These findings have important implications for plant breeding programs.
Article
Agronomy
Alessandro Rossi, Clarissa Clemente, Silvia Tavarini, Luciana G. Angelini
Summary: The use of suitable species and varieties is crucial for improving resource use efficiency, biodiversity, and agroecosystem resilience in organic cropping systems. A 2-year field trial was conducted in Central Italy to investigate the potential of linseed inclusion within organic farming. The study found that autumn sowing with milder and wetter conditions was more favorable for linseed cultivation, resulting in higher seed yield and oil content. The choice of the most suitable variety was influenced by both sowing date and location. The results suggest that linseed can be a valuable autumn alternative for diversifying organic cropping systems.
Article
Biotechnology & Applied Microbiology
Saba Delfan, Mohammad Reza Bihamta, Seyed Taha Dadrezaei, Alireza Abbasi, Hadi Alipoor
Summary: This study aimed to identify leaf rust resistance-related genomic loci in Iranian wheat cultivars and landraces through GWAS. Evaluation of 320 wheat accessions against four prevalent rust pathotypes revealed diversity in their responses. GWAS analysis identified 80 leaf rust resistance QTLs located on almost all chromosomes, with six novel loci conferring resistance. The GBLUP genomic prediction model showed superior performance, suggesting its potential for genomic selection in wheat.
Correction
Biotechnology & Applied Microbiology
Saba Delfan, Mohammad Reza Bihamta, Seyed Taha Dadrezaei, Alireza Abbasi, Hadi Alipour
Article
Agronomy
Clarissa Clemente, Leonardo Ercolini, Alessandro Rossi, Lara Foschi, Nicola Grossi, Luciana G. Angelini, Silvia Tavarini, Nicola Silvestri
Summary: The spectral response of camelina under different N fertilization regimes was evaluated using remote and proximal sensing techniques. The results showed that N fertilization significantly affected seed yield and N use efficiency, with the best results obtained at basal dressing at sowing (T2). The rise in air temperatures in 2022 led to a reduction in plant development and seed yield. The study also demonstrated the feasibility of utilizing remote sensing techniques from UAVs for predicting seed yield in camelina.
Article
Food Science & Technology
Alessandro Bianchi, Francesca Venturi, Angela Zinnai, Isabella Taglieri, Basma Najar, Monica Macaluso, Giorgio Merlani, Luciana Gabriella Angelini, Silvia Tavarini, Clarissa Clemente, Chiara Sanmartin
Summary: Avanzi 3-Grano 23 (G23) is an old variety of Triticum aestivum from Lunigiana, Italy, where traditional farming communities have contributed to its success. G23 flour has unique nutritional and sensory traits but is not suitable for breadmaking due to technological limitations. This study explores the optimization of bread formulation using flour blending and leavening systems, finding that sourdough bread made with G23 flour has improved sensory and phytochemical profiles compared to traditional bread.
Article
Agriculture, Multidisciplinary
Alireza Eslami-Firouzabadi, Mojtaba Karimi, Ali Abbasi-Surki, Alireza Shafeinia, Fatemeh Derikvand-Moghadam
Summary: The study aimed to determine the optimum N fertiliser rate and application stages for stevia production. Based on the results, it is recommended to split 40 kg N ha(-1) into two stages for stevia production under greenhouse conditions.
SOUTH AFRICAN JOURNAL OF PLANT AND SOIL
(2023)
Article
Horticulture
Marco Santin, Maria Calogera Sciampagna, Alessia Mannucci, Martina Puccinelli, Luciana Gabriella Angelini, Silvia Tavarini, Mattia Accorsi, Luca Incrocci, Annamaria Ranieri, Antonella Castagna
Summary: This study revealed the effects of pre-harvest ultraviolet-B irradiation on the nutritional and nutraceutical quality of flaxseed sprouts and microgreens. The UV-B treatment enhanced total phenolics and flavonoids content, antioxidant capacity, and quality of microgreens. However, it also decreased biomass and stem length, and caused changes in some photosynthetic pigments.
Article
Virology
Mahsa Mansourpour, Romain Gallet, Alireza Abbasi, Stephane Blanc, Akbar Dizadji, Jean-Louis Zeddam
Summary: This study investigates the impact of SCSA 1 on various traits of FBNYV in its host plant and aphid vector. The results show that SCSA 1 changes the relative amounts of virus genomic segments and increases the rate of plant-to-plant transmission.
JOURNAL OF VIROLOGY
(2022)
Article
Horticulture
Martina Puccinelli, Rita Maggini, Luciana G. Angelini, Marco Santin, Marco Landi, Silvia Tavarini, Antonella Castagna, Luca Incrocci
Summary: This study investigated the effect of light with different spectra on the performance of flax microgreens and sprouts, and found that microgreens had higher nutrient content, antioxidant capacity, and health-promoting compounds compared to sprouts.
