Journal
JOURNAL OF SEPARATION SCIENCE
Volume 37, Issue 3, Pages 281-286Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201301065
Keywords
Food safety; HPLC; Ion pair chromatography; Molecularly imprinted polymers; Mycotoxins
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A new LC method to detect fusaric acid (FA) in maize is reported based on a molecularly imprinted SPE clean-up using mimic-templated molecularly imprinted polymers. Picolinic acid was used as a toxin analog for imprinting polymers during a thermolytic synthesis. Both acidic and basic functional monomers were predicted to have favorable binding interactions by MP2 ab initio calculations. Imprinted polymers synthesized with methacrylic acid or 2-dimethylaminoethylmethacrylate exhibited imprinting effects in SPE analysis. FA levels were determined using RP ion-pairing chromatography with diode-arrayUV detection and tetrabutylammonium hydrogen sulfate in the mobile phase. A method was developed to detect FA in maize using molecularly imprinted SPE analysis within the range of 1-100 mu g/g with recoveries between 83.9 and 92.1%.
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