Journal
JOURNAL OF SEPARATION SCIENCE
Volume 36, Issue 20, Pages 3457-3462Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201300518
Keywords
Mangiferin; Mango leaves; Microwave-assisted extraction; Response surface methodology
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Funding
- Scientific Research Foundation of Guangdong Medical College [B2012009]
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Mangiferin is the main bioactive component in mango leaves, which possesses anti-inflammatory, antioxidative, antidiabetic, immunomodulatory, and antitumor activities. In the present study, a microwave-assisted extraction method was developed for the extraction of mangiferin from mango leaves. Some parameters such as ethanol concentration, liquid-to-solid ratio, microwave power, and extraction time were optimized by single-factor experiments and response surface methodology. The optimal extraction conditions were 45% ethanol, liquid-to-solid ratio of 30:1 (mL/g), and extraction time of 123 s under microwave irradiation of 474 W. Under optimal conditions, the yield of mangiferin was 36.10 +/- 0.72 mg/g, significantly higher than that of conventional extraction. The results obtained are beneficial for the full utilization of mango leaves and also indicate that microwave-assisted extraction is a very useful method for extracting mangiferin from plant materials.
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