4.5 Article

Pharmacokinetic study of schisandrin, schisandrol B, schisantherin A, deoxyschisandrin, and schisandrin B in rat plasma after oral administration of Shengmaisan formula by UPLC-MS

Journal

JOURNAL OF SEPARATION SCIENCE
Volume 36, Issue 3, Pages 485-491

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201200887

Keywords

Multiple components; Pharmacokinetic; Shengmaisan; Traditional Chinese medicine; UPLC-Q-TOF/MS

Funding

  1. Key Program of the Natural Science Foundation of the State [90709019]
  2. National Key Program on the Subject of Drug Innovation [2009ZX09502-005]
  3. National Specific Program on the Subject of Public Welfare [200807014]
  4. National Program for Key Basic Research Projects in China [2005CB523406]

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Shengmaisan (SMS) is a traditional Chinese medicine prescription widely used for the treatment of cardiovascular diseases in Asia. Its lignans are major components responsible for therapeutic action. A rapid and specific UPLC-Q-TOF/MS has been developed and validated for simultaneous quantification of the five main bioactive components, i.e. schisandrin, schisandrol B, schisantherin A, deoxyschisandrin, and schisandrin B, in rat plasma after oral administration of SMS. All calibration curves showed excellent linearity within the test ranges. Validation proved the repeatability of the method was good and recovery was satisfactory. The separation of these compounds was carried out on a Waters ACQUITY HSS T-3 column (2.1 x 100 mm, 1.8 mu m) by linear gradient elution using a mobile phase consisting of 0.01% formic acid in water and ACN containing 0.01% formic acid. In this work, plasma pharmacokinetic characteristics of lignans components after oral administration SMS were investigated using UPLC-Q-TOF/MS method. MS was performed on a Waters Micromass high-definition technology with an ESI source. Data were analyzed and estimated by compartmental methods and pharmacokinetic parameters calculated using WinNonlin Professional version 6.1. Results demonstrated that the proposed UPLC-Q-TOF/MS method was successfully applied to pharmacokinetic study of all components in rat plasma after oral administration of the SMS.

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