Optimization of a refolding step for a therapeutic fusion protein in the quality by design (QbD) paradigm

High pH Solubilization and Chromatography-Based Renaturation and Purification of Recombinant Human Granulocyte Colony-Stimulating Factor from Inclusion Bodies

(2012) Ming Li et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Size-exclusion simulated moving bed chromatographic protein refolding

(2010) Esteban J. Freydell et al.   CHEMICAL ENGINEERING SCIENCE

A high-throughput protein refolding screen in 96-well format combined with design of experiments to optimize the refolding conditions

(2010) Vincent Dechavanne et al.   PROTEIN EXPRESSION AND PURIFICATION

Use of the design-of-experiments approach for the development of a refolding technology for progenipoietin-1, a recombinant human cytokine fusion protein fromEscherichia coliinclusion bodies

(2009) Denis M. Boyle et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Effect of Additives on Protein Aggregation

(2009) Hiroyuki Hamada et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Quality by design for biopharmaceuticals

(2009) Anurag S Rathore et al.   NATURE BIOTECHNOLOGY

Cooperative effects of urea and l-arginine on protein refolding

(2009) Jing Chen et al.   PROTEIN EXPRESSION AND PURIFICATION

Efficient refolding of recombinant lipase from Escherichia coli inclusion bodies by response surface methodology

(2009) Neda Akbari et al.   PROTEIN EXPRESSION AND PURIFICATION

Roadmap for implementation of quality by design (QbD) for biotechnology products

(2009) Anurag S. Rathore   TRENDS IN BIOTECHNOLOGY

A purification method for improving the process yield and quality of recombinant human granulocyte colony-stimulating factor expressed in Escherichia coli and its characterization

(2008) Dasari Venkata Krishna Rao et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

High Recovery Refolding of rhG-CSF from Escherichia coli, Using Urea Gradient Size Exclusion Chromatography

(2008) C. Wang et al.   BIOTECHNOLOGY PROGRESS

Defining Process Design Space for Biotech Products: Case Study of Pichia pastoris Fermentation

(2008) J. Harms et al.   BIOTECHNOLOGY PROGRESS

Solubilization at high pH results in improved recovery of proteins from inclusion bodies ofE. coli

(2008) Surinder M. Singh et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Process development for production of human granulocyte-colony stimulating factor by high cell density cultivation of recombinant Escherichia coli

(2008) Rasoul Khalilzadeh et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Evaluation of refolding conditions for a human recombinant fusion cytokine protein, promegapoietin-1a

(2007) Denis M. Boyle et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY