Journal
JOURNAL OF SEPARATION SCIENCE
Volume 35, Issue 21, Pages 2978-2985Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201200433
Keywords
Adsorbent polymer; Affinity chromatography; Composite; Con A; Cryogel; Glycoprotein
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Funding
- European Union [PIAP-GA-218242]
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Composite cryogels containing porous adsorbent particles were prepared under cryogelation conditions. The composites with immobilized concanavalin A (Con A) were used for capturing glycoproteins. Adsorbent particles were introduced into the structure in order to improve the capacity and to facilitate the handling of the particles. The monolithic composite cryogels were produced from suspensions of polyvinyl alcohol particles and porous adsorbent particles and cross-linked under acidic conditions at sub-zero temperature. The cryogels were epoxy activated and Con A was immobilized as an affinity ligand. Binding and elution of horseradish peroxidase (HRP) was studied in batch experiment and in a chromatographic setup. Increasing adsorbent concentration in composite cryogels will increase ligand density, which therefore enhances the amount of bound HRP from 0.98 till 2.9 (milligram enzyme per milliliter of gel) in the chromatographic system. The material was evaluated in 10 cycles for binding and elution of HRP.
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