Article
Plant Sciences
Na Guo, Zhengyu Fang, Qingce Zang, Yiqing Yang, Tiegui Nan, Yuping Zhao, Luqi Huang
Summary: This study systematically compared the metabolite differences between P. lobata and P. thomsonii using AFA-DESI-MSI, LC-MS, and biological activity analysis. The results revealed 52 key differential metabolites and showed that P. lobata extracts exhibited a better protective efficacy on H9c2 cells against hypoxia/reoxygenation injury compared to P. thomsonii extracts.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Chemistry, Analytical
Shuang-Li Zhao, Fei Fu, Yu-Ran Jia, Xie-An Yu, Bo-Yang Yu, Ren-Shi Li
Summary: Through various chromatographic analysis methods and mass spectrometry techniques, this study successfully detected and classified the ingredients of Angelicae Sinensis Radix from six different geographical zones, providing a convenient and reliable approach for origin tracing and quality control of Angelicae Sinensis Radix.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Analytical
Alaa Riezk, Vasin Vasikasin, Richard C. C. Wilson, Timothy M. M. Rawson, James G. G. McLeod, Rishi Dhillon, Jamie Duckers, Anthony E. G. Cass, Alison H. H. Holmes
Summary: This study aimed to establish and validate a rapid and reliable liquid chromatography-tandem mass spectrometry (LC/MS) method for the simultaneous quantification of cefiderocol and meropenem in human plasma of critically ill patients. The method involved protein precipitation using acetonitrile for sample clean-up, followed by reverse phase chromatography using triple quadrupole LC/MS. The developed method showed good linearity, accuracy, and sensitivity for both cefiderocol and meropenem, with stability at different temperatures and protein binding assessed in human plasma.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Rui Guo, Qisheng Zhong, Jiaqi Liu, Peiming Bai, Zongpeng Wang, Jieling Kou, Peijie Chen, Jie Zhang, Bo Zhang
Summary: In this study, a method called 2D-LC/TQMS was developed for simultaneous analysis of hydrophilic and hydrophobic metabolites using the combination of HILIC and RPLC. The method can detect 417 metabolites involved in various metabolic pathways with good reproducibility, linearity, and wide linear range. It was tested using different biological samples and applied to a bladder cancer case/control study, identifying 30 differential metabolites related to carbohydrate and amino acid metabolism.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Patric Lindahl, Grzegorz Olszewski, Mats Eriksson
Summary: The study evaluated and optimized the stability of uranium isotopic ratios measurements using the Agilent 8900 triple quadrupole inductively coupled plasma mass spectrometry (ICP-MS/MS) system. By adjusting the mass filter resolution settings, considerable improvement in precision and accuracy was achieved, with precision of 0.07% for long-term measurement of U-233/U-235 ratios.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2021)
Article
Environmental Sciences
Yaotian Wang, Haiyan Zhang, Xingwang Hou, Qing Zhang, Weifang Chen, Jianbo Shi, Guibin Jiang
Summary: A sensitive method for simultaneous determination of nine polyCBD congeners in shellfish was developed and applied to samples from markets in eight coastal cities in China. The results showed low contamination of HCBD in the marketed shellfish and fish, laying the groundwork for future research on the environmental behavior and exposure risks of polyCBDs in aquatic biotas.
ENVIRONMENTAL POLLUTION
(2021)
Article
Chemistry, Analytical
Wenting Bu, Liang Yang, Lei Tang, Ke Xiong, Youyi Ni, Chuting Yang, Sheng Hu
Summary: Monitoring ultra-trace levels of Tc-99 in environmental samples is crucial for assessing radioactivity and tracing oceanographic processes. We introduced a new method for measuring Tc isotopes using triple quadrupole ICP-MS/MS. By introducing O-2 gas, we were able to eliminate isobaric interference from Mo-97 and achieve high separation between Mo and Tc.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Nathaniel D. Fletcher, Benjamin T. Manard, Debra A. Bostick, William D. Bostick, Shalina C. Metzger, Brian W. Ticknor, Kayron T. Rogers, Cole R. Hexel
Summary: The analysis of impurities in a uranium ore concentrate (UOC) provides valuable information on the source, production history, and potential intended use. Concentrations of phosphorus and sulfur were determined by mass spectrometry and compared to results from a pyrohydrolysis method. The method allows for the removal of interferences in the analysis of species in UOC materials.
Article
Biochemical Research Methods
Guangyong Yang, Jingxi Guo, Hui Yuan, Lei Sun, Lina Sha
Summary: In this study, a method based on ultra-performance convergence chromatography-triple quadrupole mass spectrometry (UPC2-MS/MS) was developed to detect 63 glucocorticoids in healthy foods. The method showed good linearity, low detection limits, and better selectivity and resolution compared to the traditional reverse-phase liquid chromatography-mass spectrometry (RPLC-MS/MS) method. The study provides new technical support for assessing the risk of exposure to glucocorticoids in healthy foods.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Environmental Sciences
Jungmin Jo, Younggyu Son, JiYi Lee, Dukyoung Lee, Jeoung Hwa Shin, Yun Gyong Ahn
Summary: This study evaluated the performance of GC-QqQ (MS/MS) as an alternative to GC-HR/MS for analyzing soils contaminated with PCDDs and PCDFs. The results showed good agreement between the two methods and suggested that GC-QqQ (MS/MS) could be a cost-effective alternative for soil remediation research.
Article
Chemistry, Analytical
Xinwen Zhang, Xiang Lv, Dong Qian, Junxian Li, Yi Qian, Jiaojiao Wang, Yuyu Zhu, Jing Zhou, Hongyue Ma
Summary: This research developed a quantitative approach using peptides to assess the quality of cinobufacini preparations. 230 peptides in cinobufacini injection were identified and a quantitative method for species-specific peptides was established. The quality of cinobufacini injection was found to be generally stable and the toad skin from Shandong was of the best quality.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Food Science & Technology
Ainhoa Oller-Ruiz, Natalia Campillo, Manuel Hernandez-Cordoba, Javier Gilabert, Pilar Vinas
Summary: The study proposed using DLLME for preconcentration of lipophilic marine toxins in seawater samples. By optimizing the parameters and validating the method through recovery studies, the analysis showed that the water samples were free of the studied toxins.
Article
Biochemical Research Methods
Anke Kraemer, Peter Bauer, Felix. H. Schacher
Summary: A simple and robust reversed phase separation method was established for the separation of known impurities in bisphenol-A (BPA). The method offers shorter separation time and lower sample concentration requirements, making it suitable for frequent process monitoring. Additionally, four additional impurities were detected and identified.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Hua-Ming Xiao, Xing Yang, Feng Zheng, Sofja Tshepelevitsh, Xian Wang, Xiao-Jun Yao, Ivo Leito, Yu-Qi Feng
Summary: Chemical derivatization-assisted electrospray ionization-triple quadrupole mass spectrometry (ESI-QqQMS) is an efficient tool for quantifying low-molecular-weight molecules. This study utilized quantitative structure-activity relationship (QSAR) modeling to investigate the relationship between modifying group structures in derivatization reagents and the detection sensitivity of their derivatives during ESI-MS detection. The study found that several molecular descriptors related to hydrophobicity, electronegativity, and molecular shape were correlated with the detection sensitivity of derivatives induced by different modifying groups.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Xingcheng Gong, Wenjing Liu, Yan Cao, Rongye Wang, Naiyun Liang, Libo Cao, Jun Li, Pengfei Tu, Yuelin Song
Summary: Herbal medicines, known for their complex nature, present challenges in metabolomic analysis due to their structural diversity, wide concentration range, large polarity span, lack of authentic compounds, and frequent occurrences of isomers. This study proposes an integrated strategy to overcome technical barriers and demonstrates its applicability using a well-known herbal medicine, Peucedani Radix.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Plant Sciences
Yaru Yan, Ting Mo, Wenqian Huang, Xiping Xu, Weisheng Tian, Yingxia Wang, Yuelin Song, Jun Li, Shepo Shi, Xiao Liu, Pengfei Tu
Summary: This article reports a new glycosyltransferase UGT71BD1 that can catalyze glycosylation of diverse glycosides and produce new multi-glycosylated glycosides. These products show enhanced pharmacological activities. The study also reveals the crucial role of sugar modules in substrate recognition.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Chemistry, Applied
Ni-li Zan, Zi-Han Lu, Xin-Yu Wang, Rong-Ye Wang, Nai-Yun Liang, Hui-Xia Huo, Yun-Fang Zhao, Yue-Lin Song, Peng-Fei Tu, Jiao Zheng, Jun Li
Summary: This study isolated flavonoid enantiomers and analogues from the heartwood of Dalbergia odorifera. Their structures were determined through spectroscopic analysis and experimental data. Compound 2 exhibited an unusual structure and its biosynthetic pathway was proposed. Compounds 3-5 showed significant inhibition of nitric oxide production.
NATURAL PRODUCT RESEARCH
(2023)
Letter
Biochemical Research Methods
Han Li, Ke Zhang, Xiaoya Niu, Nian Wu, Xiaoqin Su, Pengfei Tu, Yunfang Zhao, Yuelin Song
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Yan Cao, Wei Li, Wei Chen, Xiaoya Niu, Nian Wu, Yitao Wang, Jun Li, Pengfei Tu, Jiao Zheng, Yuelin Song
Summary: The study focuses on developing a new technology to enhance the confidence of structural annotation, particularly for conjugated BAs, to help understand the physiological or pathological status of vertebrates. High-confidence structural analysis was conducted on 201 BAs, revealing significant impacts on the BA submetabolome of HepG2 cells after lithocholic acid treatment.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jing Sun, Manman Li, Yongru Wang, Chuang Chang, Yujie Pei, Kewu Zeng, Yunfang Zhao, Jiao Zheng, Yuelin Song, Pengfei Tu, Huixia Huo, Jun Li
Summary: Four new phenolic glucosides and four known ones were isolated from the leaves of Vitex negundo var. cannabifolia. Their structures were determined by comprehensive analysis and comparison. One of the compounds exhibited weak inhibition of nitric oxide production in cells.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Chemistry, Analytical
Wei Li, Yiqiu Liao, Qian Hong, Han Li, Yan Cao, Wei Chen, Juyan Liu, Pengfei Tu, Jun Li, Yuelin Song
Summary: This study assessed the potential of RPLC-MS/MS in the quality evaluation of animal-originated medicinal materials (AMMs) and established a workflow for quasi-quantitative metabolomics and bottom-up proteomics. By optimizing quantitative parameters and establishing regression calibration curves, widely quasi-quantitative metabolomics and proteomics were achieved. The study found significant differences in metabolite and protein profiles between Bufonis Venenum (BV) and toad skins, highlighting the potential of RPLC-SRM as an analytical tool for in-depth quality evaluation of AMMs.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Chao-Chao Wang, Nai-Yun Liang, Hui Xia, Rong-Ye Wang, Yun-Feng Zhang, Hui-Xia Huo, Yun-Fang Zhao, Yue-Lin Song, Jiao Zheng, Peng-Fei Tu, Jun Li
Summary: This is a photo of an interesting scene in a South African zoo. The fences are covered in ice sculptures due to the sudden cold weather, attracting the animals to gather and creating a unique and fascinating sight.
Letter
Biochemical Research Methods
Jingjing Shi, Ke Zhang, Xiaoya Niu, Nian Wu, Yunfang Zhao, Pengfei Tu, Zhizi Zhou, Yuelin Song
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2023)
Article
Integrative & Complementary Medicine
Chenxiao Shen, Yu Wang, Hui Zhang, Wei Li, Wenyue Chen, Mingqing Kuang, Yuelin Song, Zhangfeng Zhong
Summary: This study explored the active components and potential mechanisms of Rosa roxburghii Tratt fruit in the treatment of Type 2 diabetes mellitus (T2DM) using UPLC-Q-Exactive Orbitrap/MS and network pharmacology approaches. The study identified 20 bioactive ingredients in the fruit, including alphitolic acid, quercetin, and ellagic acid, as well as 13 core targets such as AKT1, TNF, SRC, and VEGFA. Rosa roxburghii Tratt fruit may play a therapeutic role in T2DM by regulating signaling pathways such as PI3K/AKT, RAS, and AGE-RAGE.
Article
Integrative & Complementary Medicine
Qian Wang, Wenjing Liu, Bo Peng, Xingcheng Gong, Jingjing Shi, Ke Zhang, Bo Li, Pengfei Tu, Jun Li, Jun Jiang, Yunfang Zhao, Yuelin Song
Summary: This study attempts to use a 2D code chart to represent the mass spectrometry data of herbal medicine, fulfilling the storage demands for chemical information. The feasibility and applicability of this method were demonstrated by testing Ganoderma lucidum. It provides a rapid and practical visual way for the authentication of herbal medicine.
Article
Biochemistry & Molecular Biology
Wei Li, Wei Chen, Xiaoya Niu, Chen Zhao, Pengfei Tu, Jun Li, Wenjing Liu, Yuelin Song
Summary: As a primary treatment for cholestasis, ursodeoxycholic acid corrects the imbalances in bile acid metabolism in a comprehensive way. The complex metabolism of ursodeoxycholic acid makes it difficult to determine whether a specific bile acid species is directly or indirectly impacted by the drug, which hinders the understanding of its therapeutic mechanism. In this study, the metabolism pattern of ursodeoxycholic acid was explored using liver microsomes. Through sequential metabolism in vitro, 20 metabolites were identified, including hydroxylated, oxidized, and epimerized products that were further metabolized to glucuronides and sulfates. The conjugation sites and structural nuclei of the metabolites were determined using mass spectrometry. This study provides insight into the direct effects of ursodeoxycholic acid administration on bile acid species and highlights the utility of sequential metabolism in vitro and squared energy-resolved mass spectrometry in characterizing metabolic pathways and identifying metabolites.
Review
Chemistry, Analytical
Yuelin Song, Qingqing Song, Wenjing Liu, Jun Li, Pengfei Tu
Summary: Currently, MS/MS is widely used in metabolomics for the identification of metabolites. However, the structural identification of isomers remains challenging due to the similarity of their MS/MS spectra. Differentiation and identification of isomers using MS/MS can be facilitated by various techniques, including chemical derivatization, ion mobility spectrometry, ion dissociation techniques, and energy-resolved MS. This tutorial provides a comprehensive summary of these cutting-edge techniques and emphasizes the importance of isomeric identification in metabolomics for analytical chemists. (c) 2023 Elsevier B.V. All rights reserved.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Letter
Biochemical Research Methods
Wenzheng Li, Ke Zhang, Wei Chen, Jun Li, Yuelin Song
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2023)
Article
Chemistry, Multidisciplinary
Ke Zhang, Xingcheng Gong, Qian Wang, Pengfei Tu, Jun Li, Yuelin Song
Summary: In recent years, proteinic drugs, especially monoclonal antibodies, have become more important than small molecule drugs, and peptide mapping through LC-MS/MS has emerged as a new method for quality control. DI-MS/MSALL shows great potential as an alternative method for peptide mapping of proteinic drugs, with faster measurement time and lower solvent consumption.
