Journal
JOURNAL OF SEPARATION SCIENCE
Volume 34, Issue 19, Pages 2739-2744Publisher
WILEY-BLACKWELL
DOI: 10.1002/jssc.201100452
Keywords
Ethyl p-hydroxybenzoate; Molecularly imprinted microspheres; Precipitation polymerization; Solid-phase extraction; Soy sample
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Funding
- Specialized Research Funds of Research on key techniques for food safety, a Key Research Program in Henan province of China [0422031200]
- Key scientific and technological project of Henan Province [112102310030]
- Education Department of Henan Province [2010A150006]
- Education Innovation Foundation for the postgraduate of Henan University of Technology [10YJS036]
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Molecularly imprinted microspheres (MIMs) were prepared by precipitation polymerization for the binding and recognition of 1,4-hydroxybenzoic acid esters. Ethyl p-hydroxybenzoate (EtPHB) was used as the template molecule, methacrylic acid as the functional monomer, ethylene dimethacrylate as the linking agent. It was evaluated by solid-phase extraction column packed with MIMs combined with liquid chromatography to determine trace preservatives including benzoic acid, methyl p-hydroxybenzoate, EtPHB, propyl p-hydroxybenzoate in food products. A solid-phase extraction based on MIM procedure was used to isolate four additives from the food matrix before quantitative analysis. The Scatchard plot suggested that the template-polymer system had two-site binding behavior with the dissociation constants of 0.3577 and 3.952 mg/g, respectively. The rebinding test, based on the molecularly imprinted solid-phase extraction column technique, showed the recoveries of soy samples spiked with four additives within 88.4-110.6%, with the relative standard deviations of 1.97-3.82%. Finally, the method was successfully applied for the analysis of parabens in foodstuff without traditional pretreatment.
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