Journal
JOURNAL OF SEPARATION SCIENCE
Volume 33, Issue 14, Pages 2109-2114Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200900841
Keywords
HPLC-MS/MS; Sildenafil; Tadalafil; Vardenafil; Yohimbine
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Funding
- National Key Technologies R&D Program of China [2006BAK02A08-7]
- Hunan Key Program for Science Technology [2009FJ1017]
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A simple and sensitive method was developed for determination of illegal adulterants (yohimbine, sildenafil, vardenafil and tadalafil) in dietary supplements by HPLC-MS/MS. The separation was achieved on a C-18 column with the mobile phase consisting of acetonitrile and 0.1% acetic acid aqueous solution with a gradient elution at a flow rate of 0.5 mL/min. The analytes were quantified and identified by two characteristic transitions using the multiple-reaction monitoring mode. The recoveries of the analytes ranged from 77.5 to 109.3% with the RSD less than 8.1% (n = 6). The method has been successfully applied to screen illegal adulterations of natural dietary supplements.
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