Journal
PLANT AND CELL PHYSIOLOGY
Volume 56, Issue 4, Pages 750-758Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcv003
Keywords
Aquaporins; Calcium; Cell hydraulic conductivity; Fluoride; NaCl; Okadaic acid
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Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
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The effects of Ca(NO3)(2), KF and okadaic acid (OA) on cell hydraulic responses to NaCl were examined in roots of Arabidopsis thaliana wild-type plants and compared with plants overexpressing plasma membrane intrinsic protein PIP2; 5. Root treatment with 10mM NaCl rapidly and sharply reduced cell hydraulic conductivity (L-p) in the wild-type Arabidopsis plants, but had no effect on L-p in Arabidopsis plants overexpressing PIP2;5, suggesting that changes in protein and aquaporin gene expression were among the initial targets responsible for the inhibition of L-p by NaCl. The down-regulation of PIP transcripts after 1 h exposure to 10mM NaCl was likely a significant factor in the reduction of L-p. The effect of NaCl on L-p in the wild-type plants was abolished when the NaCl-treated roots were subsequently exposed to 5mM KF, 5mM Ca(NO3)(2) and 5 mu M OA. The reduction of L-p by 5mM KF could not be prevented by treatment with 5mM Ca(NO3)(2) in both wild-type and PIP2;5-overexpressing plants. However, 5 mu M OA, which was added following NaCl or KF treatment, completely reversed L-p within several minutes. The results provide evidence for high sensitivity of aquaporin-mediated water transport to relatively low NaCl concentrations and point to the phosphorylation and/or dephosphorylation processes as those that are likely responsible for the protection of L-p by fluoride and calcium treatments against the effects of NaCl.
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