Article
Biochemical Research Methods
Qi Wu, Xiudan Hou, Haitao Lv, Hui Li, Liang Zhao, Hongdeng Qiu
Summary: The novel octadecylamine-derived CDs, C18-CDs, were successfully designed, synthesized, and applied in RPLC/HILIC mixed-mode chromatography, showing good separation performance for both hydrophobic and hydrophilic compounds. The C18-CDs/SiO2 column demonstrated efficient separation for various compounds under different modes, indicating its potential for further research in mixed-mode chromatography.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Qi Wu, Xiudan Hou, Xiangfei Zhang, Hui Li, Liang Zhao, Haitao Lv
Summary: Amphipathic CQDs modified silica stationary phase shows typical characteristics of RPLC/HILIC, achieving satisfactory separation of both hydrophobic and hydrophilic compounds. The retention mechanism is investigated, revealing main interactions such as hydrophobic interaction, pi-pi stacking, hydrogen-bonding and electrostatic interactions under RPLC mode. This work provides a new approach for synthesis of amphipathic CQDs with great prospects in separation science.
Article
Chemistry, Analytical
Denis K. Naplekov, Pavel Barta, Frantisek Trejtnar, Hana Sklenarova, Juraj Lenco
Summary: Radioimmunoconjugates are a promising class of therapeutics and diagnostics. In this study, RPLC and HILIC liquid chromatography were used to characterize the intermediate products of radioimmunoconjugates. The results showed that the conjugation resulted in a heterogeneous mixture, and the RPLC method successfully monitored the structural changes of the conjugated product during synthesis.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Katarina Molnarova, Ales Duris, Tomas Jecmen, Petr Kozlik
Summary: Glycoproteomics, a challenging field due to glycosylation heterogeneity, benefits from hydrophilic interaction liquid chromatography for glycopeptide separation. Comparing HILIC columns in human immunoglobulin G glycopeptide separation, it was found that different columns exhibit higher separation power for different glycoforms and can effectively elucidate the structure of isomeric glycoforms. The retention mechanism for HILIC columns is discussed based on determined chromatographic parameters.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Jing Wang, Yang Zhao, Yang Yang, Xin Chen, Yu Jin, Yanxiong Ke
Summary: In this study, hydrophilic interaction liquid chromatography (HILIC) methods and a two-dimensional reversed-phase liquid chromatography/HILIC (2D-RPLC/HILIC) for the separation of steviol glycosides (SGs) were developed. The PA column exhibited superior hydrophilicity, higher selectivity, and better resolution. HILIC-UV methods with water/acetonitrile or water/ethanol as elution systems were developed for the separation of SGs. The preparative separation of minor SGs from stevia extracts was successfully achieved using 2D-RPLC/HILIC.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Chemistry, Analytical
Wanjiao Wei, Haoyu Long, Yanjuan Liu, Yuefei Zhang, Wei Chen, Sheng Tang
Summary: In this study, a novel imine-linked COF@silica composite was prepared by in-situ growth of TAPT and DHTA monomers on the surface of SiO2-NH2. The composite material showed selective retention of structurally similar analytes through multiple types of analyte-stationary phase interactions, and demonstrated good separation selectivity and efficiency.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Matthew J. Sorensen, Mellie June Paulines, Todd D. Maloney
Summary: This study evaluated the orthogonality between ion-pair reversed phase (IP-RP), anion exchange (AEX), and hydrophilic interaction liquid chromatography (HILIC) for the separation of oligonucleotides. The results showed that coupling HILIC with either AEX or IP-RP provided the highest degree of orthogonality.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Martin Gilar, Brooke M. Koshel, Robert E. Birdsall
Summary: This study investigated the separation of a fully phosphorothioated oligonucleotide from its truncated n-1 species and selected phosphodiester impurities using ion-pair reversed-phase chromatography. The hydrophobicity of ion-pairing agents impacts the separation selectivity of n-1 species. The study found that hydrophilic interaction chromatography provided a different retention pattern and improved the chromatographic separation of the truncated and phosphodiester impurities.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Ester Peris-Garcia, Maria Jose Ruiz-Angel, Juan Jose Baeza-Baeza, Maria Celia Garcia-Alvarez-Coque
Summary: The study evaluated the prediction performance of retention models for seven HILIC columns, showing that models considering partitioning or both partitioning and adsorption provide good accuracy for both HILIC and RPLC columns. Differences or similarities between RPLC and HILIC columns of diverse nature were revealed when studying elution strength behavior with varying mobile phase composition.
Article
Biochemical Research Methods
Qianying Sheng, Ling Wang, Leyuan Zhang, Xue Wang, Shengxu Qian, Minbo Lan, Guangyan Qing, Xinmiao Liang
Summary: Natural products are complex samples that contain a wide range of compounds. Effective analytical techniques are essential for understanding and controlling the quality of these products. This study proposes a design concept of bidirectional fi-cyclodextrin (fi-CD)-modified chromatographic stationary phase for the separation of bufadienolides extracted from toad. The fi-CD-based stationary phase can be used for both hydrophilic interaction liquid chromatography (HILIC) and reversed-phase liquid chromatography (RPLC), enabling high-efficiency two-dimensional separation of bufadienolides.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Majidah Alsaeedi, Huda Alghamdi, Phyllis Hayes, Anna M. Hogan, Elizabeth S. Gilchrist, Kirsten G. Dowling, Jane A. English, Jeremy D. Glennon
Summary: Nickel (Ni) is a trace heavy metal with documented allergy and carcinogenic effects in humans. Understanding the coordination mechanisms and labile complex species of Ni(II) is crucial for understanding its biological effects. Histidine (His) is an essential amino acid that contributes to protein structure and coordination of Ni(II) ions. This study evaluated different chromatographic columns for the separation of Ni(II)-Histidine species, and the Zic-cHILIC column showed high efficiency and selectivity. The identities of the Ni(II)-Histidine species were confirmed using HILIC-ESI-MS.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Marta Passamonti, Chiem de Roos, Peter J. Schoenmakers, Andrea F. G. Gargano
Summary: In this study, polymerization mixture was optimized to synthesize poly(acrylamide-co-N,N'-methylenebisacrylamide) monolithic stationary phases for HILIC of intact proteins. The monolithic structure exhibited a permeable and polar stationary phase suitable for HILIC. The monolith showed superior separation performance and lower ion interaction compared to silica-based particulate amide phase, enabling successful hyphenation with high-resolution mass spectrometry in HILIC of intact proteins.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Tomoka Yoshii, Akihiro Sakama, Kazuyoshi Kanamori, Kazuki Nakanishi, Hiroaki Imai, Daniel Citterio, Yuki Hiruta
Summary: This article presents a study on an alkali-resistant reversed-phase packing material prepared from eggshells modified with an amphiphilic copolymer for purification of basic drugs. The material showed good selectivity and retention performance, making it effective for purifying alkaline drugs and acid-sensitive compounds.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Ye Shu, Tong Chen, Tao Liu, Hechen Xu, Jinjin He, Zhihui Cai, Wanning Zhu, Zihan Zhao, Jun Jiang
Summary: In this study, spherical silica was modified with imidazole, amide, and sulfonic acid. The modified silica was used to create novel polymerization stationary phases for hydrophilic interaction liquid chromatography (HILIC). Various characterization techniques were employed to analyze the stationary phases, and their performance was evaluated using high performance liquid chromatography. The HILIC stationary phases exhibited excellent retention properties and selectivity compared to the conventional C18 column. They were successfully applied for the separation of sulfonamides, flavonoids, and Fructus Ligustri Lucidi extracts. Overall, the prepared surface polymeric stationary phases showed great potential for the analysis of polar components and complex samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Marilyn De Graeve, Emma Van de Walle, Thomas Van Hecke, Stefaan De Smet, Lynn Vanhaecke, Lieselot Y. Hemeryck
Summary: Although the field of DNA adductomics is still in its infancy, this study evaluated different methods for sample analysis and data processing. The results showed that hydrophilic interaction coupled with high resolution mass spectrometry and thermal acidic hydrolysis in combination with solid-phase extraction provided better results for studying DNA adducts. Furthermore, quality control normalization was found to be more effective compared to other normalization strategies. Therefore, quality control normalization can be recommended as the default data normalization strategy.
ANALYTICA CHIMICA ACTA
(2023)