Journal
JOURNAL OF SEPARATION SCIENCE
Volume 32, Issue 23-24, Pages 4167-4175Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200900483
Keywords
Focus microwave-assisted extraction; GC-MS; Steroidal saponins; Tribulus terrestris
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Funding
- National Nature Sciences Funding of China [20735002, 20675016]
- Physical Education Bureau of Fujian Province [200710020, 2007Y0060]
- Fujian Provincial Colleges and Universities [2008F5032]
- Scientific Research Foundation for Young Talents of Fuzhou University [0041826260, 0041826486]
- Department of Fujian Province, China
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An improved fast method for extraction of steroidal saponins in Tribulus terrestris based on the use of focus microwave-assisted extraction (FMAE) is proposed. Under optimized conditions, four steroidal saponins were extracted from Tribulus terrestris and identified by GC-MS, which are Tigogenin (TG), Gitogenin (GG), Hecogenin (HG) and Neohecogenin (NG). One of the most important steroidal saponins, namely TG was quantified finally. The recovery of TG was in the range of 86.7-91.9% with RSD < 5.2%. The conventional heating reflux extraction was also conducted in order to validate the reliability of this new FMAE method. The yield of total steroidal saponins was 90.3% in a one-step FMAE, while the yield of 65.0% was achieved during heating reflux extraction, and the extraction time was reduced from 3 h to 5 min by using less solvent. The method was successfully applied to analyze the steroidal saponins of Tribulus terrestris from different areas of occurrence. The difference in chromatographic characteristics of steroidal saponins was proved to be related to the different areas of occurrence. The results showed that FMAE-GC-MS is a simple, rapid, solvent-saving method for the extraction and determination of steroidal saponins in Tribulus terrestris.
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