Article
Chemistry, Multidisciplinary
Morgan D. McSweeney, Limei Shen, Alexander C. DeWalle, Jordan B. Joiner, Elizabeth C. Ciociola, Dharmendra Raghuwanshi, Matthew S. Macauley, Samuel K. Lai
Summary: This study explored the effectiveness of prophylaxis with free PEG or tolerogenic PEGylated liposomes as a strategy to reduce the amount of APA induced by subsequently administered PEGylated liposomes. The results showed that a single administration of free PEG alone was capable of markedly reducing the APA response to PEG-liposomes for up to 2 months, which was comparable to, and often exceeded, interventions with different tolerogenic PEG-liposomes. These findings support further research on free PEG prophylaxis as a potential strategy to mitigate the APA response to sensitizing PEGylated therapeutics.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Multidisciplinary Sciences
Zihao Yuan, Xuejun Fan, Jay-Jiguang Zhu, Tong-Ming Fu, Jiaqian Wu, Hua Xu, Ningyan Zhang, Zhiqiang An, W. Jim Zheng
Summary: This study reveals the extensive presence of murine viral sequences in patient-derived xenografts and significant expression changes of crucial genes in samples with high virus load. It emphasizes the need to carefully evaluate the impact of viral infection on patient-derived xenografts for drug development and highlights the importance of quality control in experiments involving patient-derived xenografts.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Henry Okonta, Xi Cheng, Ritu Chakravarti, Joan Duggan
Summary: The study revealed that antibiotic treatment and viral infection significantly impact the intestinal microbiota of mice, and viral infection leads to microbial dysbiosis.
Article
Biochemistry & Molecular Biology
Amir Dashti, Sophia Sukkestad, Anna M. Horner, Margaret Neja, Zain Siddiqi, Chevaughn Waller, Jordan Goldy, Dominique Monroe, Alice Lin, Nils Schoof, Vidisha Singh, Maud Mavigner, Jeffrey D. Lifson, Claire Deleage, Marina Tuyishime, Shane D. Falcinelli, Hannah A. D. King, Ruian Ke, Rosemarie D. Mason, Nancie M. Archin, Richard M. Dunham, Jeffrey T. Safrit, Sherrie Jean, Alan S. Perelson, David M. Margolis, Guido Ferrari, Mario Roederer, Guido Silvestri, Ann Chahroudi
Summary: The main barrier to achieving an HIV cure is the presence of a replication-competent reservoir of HIV in latently infected CD4+ T cells. In this study, the effectiveness of AZD5582, an IAP antagonist, in inducing SIV reactivation was confirmed. The combination of AZD5582 with N-803, an IL-15 superagonist, showed enhanced latency reversal and reduced SIV-DNA levels. These findings suggest that further research on this therapeutic approach may contribute to achieving an HIV cure.
Article
Biotechnology & Applied Microbiology
Remo Leisi, Raphael Wolfisberg, Thomas Nowak, Oliver Caliaro, Andreas Hemmerle, Nathan J. Roth, Carlos Ros
Summary: The interaction between viruses and chromatographic resin is determined by the charge distribution, which is more relevant than pI values. Canine parvovirus showed significantly different interaction with the resin at pH 5 compared to other viruses. These findings are crucial for optimizing virus purification process design.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Veterinary Sciences
Ninnet Gomez-Romero, Carlos F. Arias, Antonio Verdugo-Rodriguez, Susana Lopez, Luis Fernando Valenzuela-Moreno, Carlos Cedillo-Pelaez, Francisco Javier Basurto-Alcantara
Summary: Bovine viral diarrhea virus (BVDV) is a significant viral pathogen in ruminants, causing a wide range of clinical manifestations and economic losses in the beef and dairy industries. Traditional vaccines have limitations, leading to research on subunit vaccines as a viable alternative for BVDV protection.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Chemistry, Medicinal
Takaki Nakajima, Kazuya Nagano, Yuka Fukuda, Yu Ishima, Hiroko Shibata, Ryo Isaka, Tian-qi Zhang, Yuya Haga, Kazuma Higashisaka, Hirofumi Tsujino, Tatsuhiro Ishida, Akiko Ishii-Watabe, Yasuo Tsutsumi
Summary: Bioconjugation with polyethylene glycol (PEG) is important for protein drug development, but proteins including PEGylated ones are prone to aggregation or subvisible particle formation under dropping stress. In this study, different lengths and numbers of PEG-OVA molecules were prepared and analyzed under dropping stress, revealing that 20 kDa PEG-OVA formed approximately three times the aggregated particle concentration compared to regular OVA solution, leading to the production of anti-PEG antibodies and clearance of PEG-OVA.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Anne M. Talkington, Morgan D. McSweeney, Timothy Wessler, Marielle K. Rath, Zibo Li, Tao Zhang, Hong Yuan, Jonathan E. Frank, M. Gregory Forest, Yanguang Cao, Samuel K. Lai
Summary: PEGylation is commonly used to prolong the circulation time of protein therapeutics and nanomedicines. However, individuals exposed to PEGylated therapeutics may develop antibodies specific to PEG, increasing the risk of hypersensitivity and accelerated drug clearance. In this study, a PBPK model was developed to investigate the impact of anti-PEG antibodies on the pharmacokinetics and biodistribution of PEGylated drugs.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Biology
Haifeng C. Xu, Ruifeng Wang, Prashant V. Shinde, Lara Walotka, Anfei Huang, Gereon Poschmann, Jun Huang, Wei Liu, Kai Stuehler, Heiner Schaal, Andreas Bergthaler, Aleksandra A. Pandyra, Cornelia Hardt, Karl S. Lang, Philipp A. Lang
Summary: Using different strains of the lymphocytic choriomeningitis virus (LCMV), Xu, Wang et al. demonstrate that slower viral propagation limits type I interferon (IFN-I) production and viral persistence in mice. This study suggests reduced viral propagation as a mechanism for immune evasion and viral persistence.
COMMUNICATIONS BIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Timothy Donnison, Joey McGregor, Senthil Chinnakannan, Claire Hutchings, Rob J. Center, Pantelis Poumbourios, Paul Klenerman, Heidi E. Drummer, Eleanor Barnes
Summary: This study aims to develop a vaccine that can generate both viral-specific B-cell and T-cell responses concurrently to target multiple HCV genotypes.
Article
Pediatrics
Limin Wang, Shuhong Liu, Chunya Wang, Min Zhu, Yupeng Guo, Pan Zhao
Summary: The aim of this study was to explore the correlation between hepatocyte proliferation and hepatitis B virus (HBV) clearance in young children with chronic HBV infection. Ki-67 expression was found to play an important role in viral clearance. This is the first confirmation of the association between hepatocyte proliferation and HBV clearance in vivo and has implications for novel therapeutic strategies against hepatitis B.
Article
Microbiology
Jane X. Yeh, Yunfan Fan, Maggie L. Bartlett, Xiaoyan Zhang, Norah Sadowski, Debra A. Hauer, Winston Timp, Diane E. Griffin
Summary: Alphaviruses are important causes of viral encephalomyelitis with no approved therapies or vaccines. Antibodies targeting the Sindbis virus E2 glycoprotein play a key role in immune-mediated clearance of the virus. Direct RNA nanopore sequencing revealed that antibody treatment can alter the production of viral RNAs, leading to decreased efficiency in viral replication and release of infectious particles. These findings suggest a novel mechanism for antibody-mediated control of virus replication in neurons.
Article
Biotechnology & Applied Microbiology
Rong Fan, Fnu Namila, Davar Sansongko, S. Ranil Wickramasinghe, Mi Jin, Dharmesh Kanani, Xianghong Qian
Summary: Constant flux virus filtration experiments were conducted to evaluate the retention behavior of minute virus of mice by four commercial virus filters. The experiments showed different virus breakthrough behaviors under different flux conditions.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Virology
Lauren E. Bunke, Clairine I. S. Larsen, Jessica N. Pita-Aquino, Isabella K. Jones, Kinjal Majumder
Summary: Parvoviruses use host proteins for efficient replication in host cells, and this study reveals that the autonomous parvovirus MVM depends on the host DNA repair protein MRE11 for replication. This finding has significant implications for understanding the pathogenesis of this virus.
JOURNAL OF VIROLOGY
(2023)
Article
Materials Science, Multidisciplinary
Sankar Rajan, K. Marimuthu, C. Balaji Ayyanar, Md Enamul Hoque
Summary: The newly developed PVA-PEG-HAp thin membrane is a biocompatible alternative for biofilms in medical applications, providing potential for bone tissue regeneration.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2022)
