Journal
JOURNAL OF SEPARATION SCIENCE
Volume 31, Issue 10, Pages 1828-1833Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200700629
Keywords
anthracyclines; capillary electrophoresis; laser-induced fluorescence; plasma; ultrafiltration
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Funding
- NHLBI NIH HHS [R01 HL069014-04, R01 HL069014] Funding Source: Medline
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL069014] Funding Source: NIH RePORTER
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Anthracyclines are chemotherapeutic drugs that are widely used in the treatment of cancers such as lung and ovarian cancers. The simultaneous determination of the anthracyclines, daunorubicin, doxorubicin and epirubicin, was achieved using CE coupled to LIF, with an excitation and emission wavelength of 488 and 560 nm, respectively. Using a borate buffer (105 mM, pH 9.0) and 30% MeOH, a stable and reproducible separation of the three anthracyclines was obtained. The method developed was shown to be capable of monitoring the therapeutic concentrations (50-50000 ng/mL) of anthracyclines. LODs of 10 ng/mL, calculated at an S/N = 3, were achieved. Using the CE method developed, the in vitro protein binding to plasma was measured by ultrafiltration, and from this investigation the estimated protein binding was determined to be in the range of 77-94%.
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