Journal
JOURNAL OF REPRODUCTION AND DEVELOPMENT
Volume 60, Issue 4, Pages 288-294Publisher
SOCIETY REPRODUCTION & DEVELOPMENT-SRD
DOI: 10.1262/jrd.2014-023
Keywords
Differentiation; Gene regulation; Pituitary; POU6F2; Retina; RPF1
Funding
- KAKENHI from the Japan Society for the Promotion of Science [21380184, 24580435]
- Institute of Science and Technology, Meiji University
- Meiji University International Institute for BioResource Research (MUIIBR)
- Meiji University
- Grants-in-Aid for Scientific Research [25860148] Funding Source: KAKEN
Ask authors/readers for more resources
Homeobox transcription factors are known to play crucial roles in the anterior lobe of the pituitary gland. During molecular cloning with the Yeast One-Hybrid System using a 5'-upstream region of the porcine Fsh beta as a bait sequence, we have cloned a cDNA encoding a partial sequence of the retina-derived POU domain factor 1 (RPF1) from the porcine pituitary cDNA library and confirmed its specific binding to the bait sequence. In situ hybridization was performed to examine localization of Rpf1 and showed that this gene is expressed in the stem/progenitor cells of the rat pituitary primordium as well as the diencephalon and retina. In addition, real-time PCR demonstrated that Rpf1 transcripts are abundant in early embryonic periods but that this is followed by a decrease during pituitary development, indicating that this factor plays a role in differentiating cells of the pituitary. The transcriptional activity of RPF1 for genes of Prop1, Prrx1 and Prrx2, which were characterized as genes participating in the pituitary stem/progenitor cells by our group, was then examined with full-length cDNA obtained from the rat pituitary. RPF1 showed regulatory activity for Prop1 and Prrx2, but not for Prrx1. These results indicate the involvement of this retina-derived factor in pituitary development.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available