Article
Multidisciplinary Sciences
Sargunadevi Sivanantham, Mahalakshmi Saravanan, Nidhi Sharma, Jayashree Shrinivasan, Ramesh Raja
Summary: This study confirms that the morphology of the inner cell mass (ICM) of the blastocyst is a stronger predictor of implantation and clinical pregnancy than that of the trophectoderm (TE), and can be utilized as a biomarker of viability.
Article
Reproductive Biology
Yoshihiro Hayashi, Shun Saito, Hanako Bai, Masashi Takahashi, Manabu Kawahara
Summary: Cellular differentiation induces morphological changes in mitochondria, with elongation observed in preimplantation embryos. The study found tissue-specific mitochondrial maturation in blastocyst embryos, with complete elongation in the trophectoderm (TE) compared to the inner cell mass (ICM).
Article
Veterinary Sciences
Cristina Cuello, Cristina A. Martinez, Josep M. Cambra, Alejandro Gonzalez-Plaza, Inmaculada Parrilla, Heriberto Rodriguez-Martinez, Maria A. Gil, Emilio A. Martinez
Summary: The study showed that vitrification altered the gene expression pattern of porcine morulae produced in vivo, leading to disruptions in metabolic pathways that may interfere with embryo quality and developmental potential.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Cell Biology
Mai-Linh Nu Ton, Daniel Keitley, Bart Theeuwes, Carolina Guibentif, Jonas Ahnfelt-Ronne, Thomas Kjaergaard Andreassen, Fernando J. Calero-Nieto, Ivan Imaz-Rosshandler, Blanca Pijuan-Sala, Jennifer Nichols, Elia Benito-Gutierrez, John C. Marioni, Berthold Gottgens
Summary: The authors constructed a morphological and molecular atlas of rabbit development and compared it with the mouse atlas, suggesting that combining both can serve as a model for studying early primate development. They reported transcriptional and chromatin accessibility profiles for over 180,000 single cells and high-resolution histology sections from rabbit embryos. By comparing the transcriptional landscape of rabbits and mice, they demonstrated how this approach can provide new insights into early mammalian development.
NATURE CELL BIOLOGY
(2023)
Article
Obstetrics & Gynecology
Eric Rhon-Calderon, Cassidy Hemphill, Lisa Vrooman, Casey Rosier, Yemin Lan, Teri Ord, Christos Coutifaris, Monica Mainigi, Richard Schultz, Marisa Bartolomei
Summary: TEBx affects the health of the placenta and embryo during early development, with some changes improving later in development and others worsening, leading to metabolic changes in adult offspring.
HUMAN REPRODUCTION
(2023)
Article
Obstetrics & Gynecology
Effrosyni Chavli, Myrthe van den Born, Cindy Eleveld, Marjan Boter, Ronald van Marion, Lies Hoefsloot, Joop Laven, Esther Baart, Diane Van Opstal
Summary: This study found that despite the presence of chromosomal mosaicism in blastocysts, a single trophectoderm (TE) biopsy can accurately predict whether the inner cell mass (ICM) consists of mostly normal or abnormal cells in the majority of cases.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Developmental Biology
Di Gao, Xin Wang, Ye-Lian Yan, Chao Li, Yong-Peng Tan, Qiu-Chen Liu, Meng-Ya Zhang, Jian Zhang, Qing-Yuan Sun, Zu-Bing Cao, Yun-Hai Zhang
Summary: circKDM5B regulates porcine early embryo development by mediating miR-128. Knockdown of circKDM5B leads to reduced blastocyst formation, decreased cell numbers, impaired tight junction assembly, and disturbed paracellular sealing within the TE epithelium. Injection of miR-128 inhibitor rescues the early development of circKDM5B knockdown embryos.
MOLECULAR HUMAN REPRODUCTION
(2023)
Article
Cell Biology
Baojiang Wu, Zhiqing Yang, Yijie Liu, Jianwen Li, Chen Chen, Xihe Li, Siqin Bao
Summary: This study used a defined medium to derive ACL-ESCs and ACL-XEN cells from one blastocyst and successfully generated ACL-blastoids by combining ACL-ESCs and ACL-XEN cells with ACL-blastoid medium in a three-dimensional culture system. The lineage markers expression analysis showed that ACL-ESCs and ACL-XEN cells represented ICM and PrE lineages. This study provides a valuable ACL culture system for deriving and maintaining two distinct cell lines and also contributes to our understanding of early embryogenesis and the application of stem cells.
CELL PROLIFERATION
(2023)
Article
Environmental Sciences
Wonhyoung Park, Whasun Lim, Gwonhwa Song
Summary: The study showed that Fipronil can reduce the viability of porcine trophectoderm and luminal epithelial cells, induce cell cycle arrest and apoptotic cell death, and affect cell migration and implantation ability. Furthermore, it was observed that Fipronil can alter the PI3K-AKT and MAPK-ERK1/2 signal transduction pathways in the treated cells.
ENVIRONMENTAL POLLUTION
(2021)
Article
Multidisciplinary Sciences
Azelle Hawdon, Niall D. Geoghegan, Monika Mohenska, Anja Elsenhans, Charles Ferguson, Jose M. Polo, Robert G. Parton, Jennifer Zenker
Summary: The spatial sorting of RNA transcripts in mouse preimplantation embryos plays a crucial role in gene expression and cell fate determination. Basally directed RNA transport is facilitated by microtubules and lysosomes. Despite higher accumulation in basal regions, higher translation activity occurs at the dispersed apical RNA foci, demonstrating spatial heterogeneities in RNA subtypes, organelle interactions, and translation events. Biased inheritance of RNA transcripts and differential translation capacity regulate cell fate allocation during embryonic development.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Mingyun Lee, Jong-Nam Oh, Gyung Cheol Choe, Seung-Hun Kim, Kwang-Hwan Choi, Dong-Kyung Lee, Jinsol Jeong, Chang-Kyu Lee
Summary: This study investigated the role of OCT4 in cell lineage specification and embryo proliferation in porcine parthenotes. The results demonstrated that OCT4 plays a crucial role in pluripotency, ICM formation, secondary cell fate decision, and cell proliferation.
CELL PROLIFERATION
(2022)
Article
Cell & Tissue Engineering
Leqian Yu, Deirdre Logsdon, Carlos A. Pinzon-Arteaga, Jialei Duan, Toshihiko Ezashi, Yulei Wei, Ana Elisa Ribeiro Orsi, Seiya Oura, Lizhong Liu, Lei Wang, Kun Liu, Xiaoyun Ding, Linfeng Zhan, Junfei Zhang, Asrafun Nahar, Caitlen Stobbe, Mandy Katz-Jaffe, William B. Schoolcraft, Tao Tan, Gary C. Hon, Ye Yuan, Jun Wu
Summary: Recent advances in human blastoids have provided a new approach for modeling early human development and implantation. An optimized protocol for efficient generation of high-fidelity human blastoids has been reported, allowing for proteomics analysis and identification of specific signatures involved in blastoid signaling states. Additionally, the effects of endometrial stromal cells on trophoblast cell survival, proliferation, and syncytialization have been uncovered. Single-cell RNA sequencing revealed similarities and differences between blastoids and blastocysts, as well as a population resembling early migratory trophoblasts during co-culture with endometrial stromal cells. This optimized protocol will facilitate broader use of human blastoids as a reliable model for human blastocysts.
Article
Endocrinology & Metabolism
Jihui Ai, Lei Jin, Yu Zheng, Peiwen Yang, Bo Huang, Xiyuan Dong
Summary: The blastocyst expansion stage, ICM grade, and TE grade are all associated with pregnancy outcomes. ICM grade is the strongest predictor of live birth. A blastocyst with stage 4-5, ICM grade A, and TE grade A/B should be given priority for single embryo transfer.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Environmental Sciences
Mohamed Ashfaque, A. Nafeez Ahmed, S. Mohammed Safiullah, G. Taju, S. Abdul Majeed, A. S. Sahul Hameed, K. Anver Basha
Summary: Carbon nanotubes (CNTs) have been widely used in developing polymer hybrid coatings for anticorrosive application. In this study, a novel poly [(3,5-dimethyl-lH-pyrazole-1-yl) methyl methacrylate-co-glycidyl methacrylate] (PyM) was prepared and single-wall carbon nanotubes (SWCNT) were incorporated in PyM to form nanocomposites. The toxicity of the nanocomposites was evaluated in vivo and in vitro using zebrafish embryos and gill cell line of zebrafish (DrG). The results showed that nanocomposites with a concentration of 400 μg/ml of PyM in 1.0% of SWCNT were non-toxic and recommended for anticorrosive application. Nanocomposites with higher SWCNT content showed toxic effects. The nanocomposites with 1.5% of SWCNT delayed hatching rate, decreased survival rate and heart beat in zebrafish embryos, and inducedmorphological changes in DrG cells. Gene expression studies revealed that nanocomposites with high SWCNT concentration induced oxidative stress. The immersion study of the recommended concentration of PyM-SWCNT on mild steel (MS) in sea water showed effective corrosion protection without leaching behavior. The findings suggest that the nanocomposites with the novel polymer have potential for industrial anticorrosive applications.
Article
Agriculture, Dairy & Animal Science
C. Alminana, F. Dubuisson, S. Bauersachs, E. Royer, P. Mermillod, E. Blesbois, F. Guignot
Summary: This study reveals the specific alterations in gene expression of embryonic pig embryos during vitrification (VIT) by comparing them with in vitro cultured (IVC) embryos and analyzing their transcriptomes. The identified VIT alterations may reflect the transcriptional signature of embryo cryodamage and the healing process overcoming the impact of VIT. Selected validated genes are potential biomarkers to improve vitrification.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)