Article
Genetics & Heredity
Corinne Loeuillet, Magali Dhellemmes, Caroline Cazin, Zine-Eddine Kherraf, Selima Fourati Ben Mustapha, Raoudha Zouari, Nicolas Thierry-Mieg, Christophe Arnoult, Pierre F. Ray
Summary: Oocyte maturation defect (OMD) is a common cause of female factor infertility, often caused by ovulatory disorders. Several genes including PATL2, TUBB8, WEE2, and ZP1 have been associated with OMD. This study identified a new frequent ZP1 variant and found degeneration and absence of oocytes in patients with ZP1 mutations.
Article
Chemistry, Analytical
Yue Du, Yizhe Chen, Shuai Zhang, Dai Cheng, Yaowei Liu, Qili Zhao, Mingzhu Sun, Maosheng Cui, Xin Zhao
Summary: This study developed a high-fidelity finite element model for describing the viscoelastic responses of oocytes under loading. By analyzing and characterizing the subcellular components of oocytes, the model accurately predicted the deformation of oocytes under different loading conditions. This model has important implications for further investigating the characteristics and behavior of oocytes.
Article
Agriculture, Dairy & Animal Science
Koki Takebayashi, Manita Wittayarat, Qingyi Lin, Maki Hirata, Naoaki Yoshimura, Nanaka Torigoe, Megumi Nagahara, Lanh Thi Kim Do, Fuminori Tanihara, Takeshige Otoi
Summary: CRISPR/Cas9 technology has greatly influenced the efficiency and effectiveness of genetic modifications. The study found that electroporation alone significantly increased the frequency of biallelic mutations, while lipofection alone required the removal of the zona pellucida to enable CRISPR/Cas9-based genome editing. Additionally, additional lipofectamine treatment did not improve the mutation rates.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Review
Biology
Paul M. Wassarman, Eveline S. Litscher
Summary: The presence of a zona pellucida (ZP) is essential for the fertility of female mammals, including mice and humans. It stabilizes the contacts between oocyte microvilli and follicle cell projections, supporting the development of growing oocytes and follicles. The absence of a ZP, due to gene inactivation or mutation, results in a loss of contacts and reduced production of ovulated eggs, leading to female infertility.
Article
Veterinary Sciences
Zhao Namula, Maki Hirata, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Naoaki Yoshimura, Fuminori Tanihara, Chommanart Thongkittidilok, Takeshige Otoi
Summary: The study showed that weakening the ZP does not affect the developmental competence, mutation rate, or mutation efficiency of electroporated zygotes, while ZP removal has a detrimental effect on embryonic development but may increase the mutation rate.
VETERINARY MEDICINE AND SCIENCE
(2022)
Article
Multidisciplinary Sciences
Yoshitaka Fujihara, Sarah Herberg, Andreas Blaha, Karin Panser, Kiyonori Kobayashi, Tamara Larasati, Maria Novatchkova, Hans-Christian Theussl, Olga Olszanska, Masahito Ikawa, Andrea Pauli
Summary: Fertilization is a crucial process initiating new individual development in sexually reproducing species. SPACA4, a sperm protein, is essential for efficient fertilization in mammals like mice, particularly for interactions with the zona pellucida. Its absence leads to subfertility in male knockout mice, with fertilization rescue observed when the zona pellucida is removed during in vitro experiments.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Carla Moros-Nicolas, Pascale Chevret, Maria Jimenez-Movilla, Blanca Algarra, Paula Cots-Rodriguez, Leopoldo Gonzalez-Brusi, Manuel Aviles, Ma Jose Izquierdo-Rico
Summary: The zona pellucida (ZP) is a ancient extracellular matrix surrounding mammalian oocytes and embryos, preventing polyspermy and protecting the embryo. Studies have shown differences in ZP composition among different mammalian species, likely due to molecular evolution of the ZP gene family. Interactions with other proteins in the oviductal lumen may play different roles in different mammalian species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Danyil Azarkh, Yuan Cao, Julia Floehr, Uwe Schnakenberg
Summary: The success of in vitro fertilization is closely related to the quality of the oocytes. The oocyte's outer membrane, called zona pellucida, undergoes dynamic changes during the reproductive cycle. Monitoring the hardening process of the zona pellucida is crucial for successful fertilization. In this study, two genetic mouse models with different zona pellucida properties were studied using a microfluidic aspiration-assisted electrical impedance spectroscopy system to evaluate the mechanical properties of oocytes.
Article
Multidisciplinary Sciences
Seulgi Mun, Mi Young Noh, Erika R. Geisbrecht, Karl J. Kramer, Subbaratnam Muthukrishnan, Yasuyuki Arakane
Summary: Chitin deacetylases (CDAs) play a crucial role in arthropod locomotion and muscle attachment. Depletion of CDAs results in tendon cuticle breakage, muscle detachment, and defective locomotion. Furthermore, CDA deficiency leads to disrupted microtubule development, loss of adhesion junctions, and abnormal morphology of tendon cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Agriculture, Dairy & Animal Science
Andrew Benson, Josh Steed, Mia Malloy, Adam J. Davis
Summary: This study examined the impact of different genetic lines and egg locations on the abundance of ZP proteins in turkeys. The results revealed differences in protein abundance and inconsistencies with previous mRNA expression findings. This research provides valuable insights into the quantitative differences in this crucial protein layer and offers potential protein markers for improving fertility in the poultry industry.
Article
Agriculture, Dairy & Animal Science
Celia Pineiro-Silva, Sergio Navarro-Serna, Ramses Belda-Perez, Joaquin Gadea
Summary: Lipofection treatment, which involves encapsulating the CRISPR/Cas9 system into vesicles and introducing them into cells, can be a simple and inexpensive method for producing genetically modified pigs. It has similar effects on embryo development and mutation rates as the electroporation technique when used in zona-pellucida-intact porcine oocytes. However, increasing the concentration of liposomes-CRISPR/Cas9 system complexes in the media has a detrimental effect on embryo development parameters, possibly indicating a toxic effect.
Article
Chemistry, Multidisciplinary
Jiangsheng Xu, James G. Shamul, Nicholas A. Staten, Alisa M. White, Bin Jiang, Xiaoming He
Summary: This study presents a biomimetic microencapsulation approach for isolating and culturing high-quality human iPSCs, resulting in pluripotent iPSC spheroids with significantly improved quality. Hyaluronic acid (HA) is identified as crucial for isolating high-quality iPSCs in the biomimetic core-shell microencapsulation culture, and the isolated iPSCs demonstrate high pluripotency even after being cultured again in 2D. These findings and the bioinspired culture method have potential benefits for advancing human iPSC-based personalized medicine.
Article
Biochemistry & Molecular Biology
Keisuke Shimizu, Takeshi Takeuchi, Lumi Negishi, Hitoshi Kurumizaka, Isao Kuriyama, Kazuyoshi Endo, Michio Suzuki
Summary: This study investigates the evolutionary process of shell matrix proteins in mollusks and reveals the different expression locations of various proteins related to shell formation.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Cell Biology
Bo Yu, Helena T. A. van Tol, Tom A. E. Stout, Bernard A. J. Roelen
Summary: This study confirms the presence of cellular fragments in the perivitelline space of bovine IVP blastocysts through mechanical hatching, but this is not an indicator of embryo quality. Surprisingly, there is no significant difference in embryo quality between expanded blastocysts with and without cellular fragments in the perivitelline space.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Weihong Fan, Tengda Huang, Tian Wu, Hanako Bai, Manabu Kawahara, Masashi Takahashi
Summary: The removal of the zona pellucida by acid Tyrode's solution in mouse embryos affects the expression pattern of inner cell mass/trophectoderm-related genes and post-implantation development.
BIOLOGY OF REPRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Hafiza Khatun, Junki Egashira, Miki Sakatani, Naoki Takenouchi, Hideki Tatemoto, Yasuhiko Wada, Ken-ichi Yamanaka
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2018)
Article
Reproductive Biology
Ken-ichi Yamanaka, Hafiza Khatun, Junki Egashiraa, Ahmed Zaky Balboula, Hideki Tatemoto, Miki Sakatani, Naoki Takenouchi, Yasuhiko Wada, Masashi Takahashi
Article
Agriculture, Dairy & Animal Science
Khin Mar Lay, Tadashi Nakada, Hideki Tatemoto
ANIMAL SCIENCE JOURNAL
(2013)
Article
Agriculture, Dairy & Animal Science
Ken-Ichi Yamanaka, Kyoko Yamashita, Hafiza Khatun, Yasuhiko Wada, Hideki Tatemoto, Miki Sakatani, Naoki Takenouchi, Masashi Takahashi, Shinya Watanabe
ANIMAL SCIENCE JOURNAL
(2018)
Article
Agriculture, Dairy & Animal Science
M. U. Ahammad, C. Nishino, H. Tatemoto, N. Okura, S. Okamoto, Y. Kawamoto, T. Nakada
BRITISH POULTRY SCIENCE
(2013)
Article
Agriculture, Dairy & Animal Science
M. U. Ahammad, C. Nishino, H. Tatemoto, N. Okura, S. Okamoto, Y. Kawamoto, T. Nakada
Article
Reproductive Biology
Muslah U. Ahammad, C. Nishino, H. Tatemoto, N. Okura, Y. Kawamoto, S. Okamoto, T. Nakada
Article
Reproductive Biology
Kazuki Shimokawa, Ryuko Oshiro, Kenichi Yamanaka, Koji Ashizawa, Shigeo Ohta, Hideki Tatemoto
Article
Reproductive Biology
Koji Ashizawa, Nao Oyama, Seiichi Katayama, Kazunori Narumi, Hideki Tatemoto, Yasuhiro Tsuzuki
Article
Reproductive Biology
Hafiza Khatun, Yusuke Ihara, Kota Takakura, Junki Egashira, Yasuhiko Wada, Toshihiro Konno, Hideki Tatemoto, Ken-ichi Yamanaka
Article
Developmental Biology
Hafiza Khatun, Yasuhiko Wada, Toshihiro Konno, Hideki Tatemoto, Ken-ichi Yamanaka
Article
Reproductive Biology
Hideki Tatemoto, Naoto Osokoshi, Moe Hirai, Yusuke Masuda, Toshihiro Konno, Kenichi Yamanaka
Summary: The addition of L-carnitine to the freezing extender effectively improves the quality of cryopreserved Okinawan native Agu pig sperm by enhancing sperm motility, maintaining mitochondrial and plasmalemmal integrities, and increasing proteolytic activity of acrosomal contents. It also enhances the penetrability of sperm into matured oocytes. This study highlights the importance of preventing mitochondrial dysfunction caused by oxidative stress during sperm cryopreservation.
Article
Agriculture, Dairy & Animal Science
Yusuke Masuda, Theerapat Kheawkanha, Ayari Nagahama, Kokoro Kawasaki, Toshihiro Konno, Kenichi Yamanaka, Hideki Tatemoto
Summary: This study evaluated the improvements in freezing extender cryoprotective activity by adding antifreeze protein (AFP) to prevent physical cell damage during sperm cryopreservation. The addition of AFP III significantly improved sperm motility, mitochondria and cell membrane integrity, and acrosomal proteolytic activity after thawing in 5 out of 6 individuals. Furthermore, AFP III effectively enhanced sperm viability, DNA quality, and in vitro sperm penetrability compared to AFP I treatment.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Junki Egashira, Yusuke Ihara, Hafiza Khatun, Yasuhiko Wada, Toshihiro Konno, Hideki Tatemoto, Ken-ichi Yamanaka
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2019)