Article
Multidisciplinary Sciences
P. Olof Olsson, Jeong Yeonwoo, Kyumi Park, Yeong-Min A. Yoo, W. S. A. Hwang
Summary: The successful cloning of canine pups from urine-derived cells (UDCs) has been reported. UDCs are easily obtainable and noninvasive, and can be cultured and transfected with plasmids. These cells can be used as a source material for noninvasive recovery of breeding capacity in genetically important or infertile animals, as well as for human and animal studies.
Article
Chemistry, Multidisciplinary
Xue-Han Xu, Tie-Jun Yuan, Haseeb Anwar Dad, Mu-Yang Shi, Yi-Yu Huang, Zhi-Hong Jiang, Li-Hua Peng
Summary: Exosomes derived from ginseng were shown to efficiently promote neural differentiation of BMSCs by transferring miRNAs, demonstrating potential for neural regenerative medicine both in vitro and in vivo.
Article
Biochemistry & Molecular Biology
Du-Hyong Cho, Yun-Jin Hwang, Jin Hee Park, Jee Young Lee, Jung-Hyun Park, Inho Jo
Summary: This study developed an organelle-specific, peptide-based NO delivery material that targets the nucleus. The delivery concept can be extended to cells other than stem cells to explore the effects of NO release in the nucleus. Furthermore, NO can be delivered to other organelles such as mitochondria and lysosomes through conjugation with specific targeting signals.
BIOORGANIC CHEMISTRY
(2023)
Article
Anatomy & Morphology
Mohsen Ghiasi, Mehrdad Hashemi, Ali Salimi, Khosrow Jadidi, Mahmood Tavallaie, Hossein Aghamollaei
Summary: The study aimed to differentiate hADSCs into corneal keratocyte cells using natural scaffolds and conditioned medium, and evaluate their safety in the cornea. The results showed that implantation of differentiated cells in the cornea showed no serious complications. This study suggests that the combination of corneal extracellular matrix and KCM can induce hADSCs differentiation into corneal cells, providing an alternative method for corneal tissue engineering.
Article
Chemistry, Multidisciplinary
Soo-Jin Son, SeokJin Jang, HyungChul Rah, SeokHwa Choi
Summary: Miniature pig DPSCs and PDLSCs exhibit higher proliferation rates compared to domestic farm pig breeds, but show lower osteogenic abilities.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Analytical
Yadi Wang, Wentao Dai, Zhixiao Liu, Jixiang Liu, Jie Cheng, Yuanyuan Li, Xueling Li, Jun Hu, Junhong Lu
Summary: A method based on single-cell FTIR microspectroscopy was developed to quantitatively evaluate cellular heterogeneity by calculating the cell-to-cell similarity distance, revealing that infrared phenotypes may reflect dynamic heterogeneity changes in cell populations. This provides an alternative label-free optical approach for quantifying cellular heterogeneity and can be combined with other single-cell analysis tools to understand the genotype-to-phenotype relationship in cellular populations.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Yaqiong Yu, Yoko Uchida-Fukuhara, Yao Weng, Yuhan He, Mika Ikegame, Ziyi Wang, Kaya Yoshida, Hirohiko Okamura, Lihong Qiu
Summary: In this study, the roles of NRP1 and GAG-modifiable NRP1 in adipogenesis were explored in C3H10T1/2 cells. The expression of full-length NRP1 and GAG-modifiable NRP1 increased during adipogenic differentiation, and NRP1 knockdown repressed adipogenesis. JIP4 was found to be involved in adipogenesis by interacting with NRP1. Moreover, the non-GAG-modifiable NRP1 mutant greatly promoted adipogenic differentiation by activating the Akt and ERK1/2 pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Anett Mazlo, Ramona Kovacs, Noemi Miltner, Marta Toth, Zoltan Vereb, Krisztina Szabo, Ildiko Bacskai, Kitti Pazmandi, Agota Apati, Tamas Biro, Krisztian Bene, Eva Rajnavolgyi, Attila Bacsi
Summary: Findings suggest that MSCl cells promote differentiation of CTLA-4 expressing DCs through the production of all-trans retinoic acid, leading to the activation and polarization of allogeneic, naive T cells by semi-matured DCs in a CTLA-4 dependent manner. Understanding the molecular mechanisms behind MSC-mediated modulation of DC differentiation may expand the clinical application of MSCs in cell-free therapies.
Article
Plant Sciences
Avinash Sanap, Kalpana Joshi, Tejas Shah, Girish Tillu, Ramesh Bhonde
Summary: The study showed that Piper longum L. fruit extract significantly increased proliferation of WJMSCs and enhanced Ca2+ accumulation and matrix mineralization, along with promoting alkaline phosphatase (ALP) activity and the expression of key gene mRNAs. This suggests that the fruit extract can enhance osteogenic differentiation of WJMSCs, potentially serving as an anti-osteoporotic agent.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Seon-Ung Hwang, Kiyoung Eun, Mirae Kim, Junchul David Yoon, Lian Cai, Hyerin Choi, Dongjin Oh, Gabsang Lee, Hyunggee Kim, Eunhye Kim, Sang-Hwan Hyun
Summary: This study demonstrates successful establishment of neuro-organoids derived from porcine embryonic stem-like cells in vitro. The protocol developed in this study can serve as a tool for drug development, patient-specific chemotherapy, and human central nervous system disease studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Maria Rosaria Ambrosio, Teresa Migliaccio, Fabiana Napolitano, Sarah Di Somma, Giovanni Maneli, Jussara Amato, Bruno Pagano, Antonio Randazzo, Giuseppe Portella, Pietro Formisano, Anna Maria Malfitano
Summary: In this study, the role of G4 motifs in adipogenic differentiation was investigated using human adipose-derived mesenchymal stem cells (ASCs). The results showed that G4 structures were increased in differentiated adipocytes compared to their precursors, and treatment with the G4 ligand Braco-19 reduced G4 content in the mature adipocytes. Furthermore, Braco-19 caused morphological changes and reduced lipid droplet formation and key gene expression in the mature adipocytes. These findings reveal a new role of G4 motifs in human adipocyte differentiation.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell & Tissue Engineering
Changzhen Wang, Hongmei Ning, Jiao Gao, Teng Xue, Ming Zhao, Xiaoxia Jiang, Xiaoming Zhu, Ximin Guo, Hong Li, Xiaoyan Wang
Summary: This study investigated the impact of hematopoietic cells on MSCs and found that partial deletion of the Pten gene in hematopoietic cells can lead to abnormal differentiation of MSCs. There is a reciprocal relationship between hematopoietic cells and MSCs, with an important role in bone development.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Multidisciplinary Sciences
Lixia Zhao, Xuefei Gao, Yuxuan Zheng, Zixin Wang, Gaoping Zhao, Jie Ren, Jia Zhang, Jian Wu, Baojiang Wu, Yanglin Chen, Wei Sun, Yunxia Li, Jie Su, Yulin Ding, Yuan Gao, Moning Liu, Xiaochun Bai, Liangzhong Sun, Guifang Cao, Fuchou Tang, Siqin Bao, Pentao Liu, Xihe Li
Summary: The researchers have successfully established stem cells from porcine and human preimplantation embryos using the EPSC approach, enabling cross-species differentiation. Upon studying bovine EPSCs, they found that these stem cells express high levels of pluripotency genes in feeder-free culture and maintain genetic stability in long-term culture.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Maria Mesuraca, Clelia Nistico, Nicola Lombardo, Giovanna Lucia Piazzetta, Nadia Lobello, Emanuela Chiarella
Summary: Killian’s (antrochoanal) polyp is a unilateral nasal polypoid lesion that mainly affects children and young adults, with unclear etiology but potentially linked to chronic inflammation, autoimmune reactions, allergies, and viral infections. The study isolated and cultured mesenchymal stem cells from surgical biopsies of patients with this condition, revealing differences in cell proliferation, migration, and differentiation potential between KNP-MSCs and HNT-MSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Juan C. Henao, Adriana Grismaldo, Alfonso Barreto, Viviana M. Rodriguez-Pardo, Claudia Camila Mejia-Cruz, Efrain Leal-Garcia, Rafael Perez-Nunez, Patricio Rojas, Ramon Latorre, Ingrid Carvacho, Yolima P. Torres
Summary: The study demonstrates that TRPM8 channels are expressed in human bone marrow MSCs and are involved in osteogenic differentiation, with agonists promoting osteogenic differentiation and antagonists leading to a decrease in osteogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Sun-A Ock, Geun-Ho Maeng, Yeon-Mi Lee, Tae-Ho Kim, B. Mohana Kumar, Sung-Lim Lee, Gyu-Jin Rho
CELL TRANSPLANTATION
(2013)
Article
Genetics & Heredity
B. Mohana Kumar, Geun Ho Maeng, Ryoung Hoon Jeon, Yeon Mi Lee, Won Jae Lee, Byeong Gyun Jeon, Sun A. Ock, Gyu Jin Rho
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2012)
Article
Engineering, Biomedical
Jin Ho Lee, Shin-Won Kim, Uk-Kyu Kim, Se Heang Oh, Sang June-Kim, Bong-Wook Park, Jin-Hyun Kim, Young-Sool Hah, Deok Ryong Kim, Gyu-Jin Rho, Geun-Ho Maeng, Ryoung-Hoon Jeon, Hee-Chun Lee, Jong-Ryoul Kim, Gyoo-Cheon Kim, June-Ho Byun
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2013)
Article
Cell & Tissue Engineering
Bong-Wook Park, Dong-Ho Kang, Eun-Ju Kang, June-Ho Byun, Jong-Sil Lee, Geun-Ho Maeng, Gyu-Jin Rho
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2012)
Meeting Abstract
Developmental Biology
J. H. Lee, Y. M. Lee, G. H. Maeng, R. H. Jeon, T. H. Kim, W. J. Lee, S. A. Ock, B. G. Jeon, B. Mohana Kumar, S. L. Lee, G. J. Rho
REPRODUCTION FERTILITY AND DEVELOPMENT
(2012)
Meeting Abstract
Developmental Biology
W. J. Lee, G. H. Maeng, R. H. Jeon, G. J. Rho, S. L. Lee
REPRODUCTION FERTILITY AND DEVELOPMENT
(2012)
Meeting Abstract
Developmental Biology
S. A. Ock, G. H. Maeng, Y. M. Lee, T. H. Kim, B. M. Kumar, S. L. Lee, G. J. Rho
REPRODUCTION FERTILITY AND DEVELOPMENT
(2012)
Meeting Abstract
Developmental Biology
T. H. Kim, Y. M. Lee, G. H. Maeng, J. H. Lee, R. H. Jeon, S. A. Sun, B. Mahana Kumar, B. G. Jeon, W. J. Lee, S. L. Lee, G. J. Rho
REPRODUCTION FERTILITY AND DEVELOPMENT
(2012)
Article
Veterinary Sciences
B. Mohana Kumar, Geun-Ho Maeng, Yeon-Mi Lee, Tae-Ho Kim, Jeong-Hyeon Lee, Byeong-Gyun Jeon, Sun-A Ock, Jae-Gyu Yoo, Gyu-Jin Rho
RESEARCH IN VETERINARY SCIENCE
(2012)
Article
Veterinary Sciences
B. Mohana Kumar, Geun-Ho Maeng, Yeon-Mi Lee, Jeong-Hyeon Lee, Byeong-Gyun Jeon, Sun-A Ock, Taeyoung Kang, Gyu-Jin Rho
VETERINARY RESEARCH COMMUNICATIONS
(2013)
Article
Biotechnology & Applied Microbiology
Satyabrata Das, Naoko Koyano-Nakagawa, Ohad Gafni, Geunho Maeng, Bhairab N. Singh, Tara Rasmussen, Xiaoyan Pan, Kyung-Dal Choi, Daniel Mickelson, Wuming Gong, Pruthvi Pota, Cyprian V. Weaver, Stefan Kren, Jacob H. Hanna, Demetris Yannopoulos, Mary G. Garry, Daniel J. Garry
NATURE BIOTECHNOLOGY
(2020)
Article
Developmental Biology
Geunho Maeng, Wuming Gong, Satyabrata Das, Demetris Yannopoulos, Daniel J. Garry, Mary G. Garry
Article
Engineering, Biomedical
Geunho Maeng, Satyabrata Das, Sarah M. Greising, Wuming Gong, Bhairab N. Singh, Stefan Kren, Daniel Mickelson, Erik Skie, Ohad Gafni, Jacob R. Sorensen, Cyprian V. Weaver, Daniel J. Garry, Mary G. Garry
Summary: Through CRISPR technology, deletion of MYF5, MYOD and MYF6 genes in pig embryos can lead to the production of intraspecies and interspecies chimaeric pig embryos lacking native skeletal muscle, followed by somatic-cell nuclear transfer and the delivery of exogenous cells through blastocyst complementation. The generated chimaeric pig embryos exhibited normal histology, morphology and function, showing potential for research and xenotransplantation of exogenic organs. Furthermore, human:pig chimaeras created with TP53-null human induced pluripotent stem cells demonstrated higher chimaerism efficiency, with humanized muscle observed in embryos at different embryonic stages.
NATURE BIOMEDICAL ENGINEERING
(2021)
Article
Veterinary Sciences
Ryoung-Hoon Jeon, Geun-Ho Maeng, Won-Jae Lee, Tae-Ho Kim, Yeon-Mi Lee, Jeong-Hyeon Lee, Basavarajappa-Mohana Kumar, Sung-Lim Lee, Gyu-Jin Rho
JAPANESE JOURNAL OF VETERINARY RESEARCH
(2012)
Article
Oncology
Byeong-Gyun Jeon, B. Mohana Kumar, Eun-Ju Kang, Geun-Ho Maeng, Yeon-Mi Lee, Young-Sool Hah, Sun-A Ock, Dae-Oh Kwack, Bong-Wook Park, Gyu-Jin Rho
ANTICANCER RESEARCH
(2011)