Article
Reproductive Biology
Van Khanh Nguyen, Tamas Somfai, Daniel Salamone, Vu Thi Thu Huong, Huong Le Thi Nguyen, Quan Xuan Huu, Au Thi Hoang, Hieu Trung Phan, Yen Kim Thi Pham, Lan Doan Pham
Summary: An efficient culture system was established to produce embryos by SCNT of the endangered Vietnamese ? pig, achieving a blastocyst production rate of 26.4% by improving culture systems for donor cells, oocytes, and embryos culture. Transfer of embryos resulted in pregnancies, but live offspring were not obtained.
Article
Cell Biology
Jin-Song An, Guo-Liang Wang, Dong-Ming Wang, Yong-Qing Yang, Jia-Shun Wu, Ying-Qi Zhao, Shuai Gong, Jing-He Tan
Summary: This study found that corticotropin-releasing hormone (CRH) and cortisol indirectly impair pig fertilization and embryo development by inducing apoptosis in pig oviductal epithelial cells (OECs) through the activation of the FAS and TNF alpha systems. Adrenocorticotropic hormone (ACTH) did not show any detrimental effect on pig embryos or OECs.
Article
Cell Biology
Zhaojun Geng, Yongxun Jin, Fushi Quan, Siyi Huang, Shuming Shi, Bing Hu, Zhichao Chi, Ilkeun Kong, Mingjun Zhang, Xianfeng Yu
Summary: This study found that MXC has significant effects on the development of early porcine embryos, leading to decreased cleavage rate, blastocyst rate, hatching rate, and total cell count. Moreover, MXC increases the production of reactive oxygen species, decreases mitochondrial membrane potential, and reduces mitochondrial copy number during early embryo development. Furthermore, MXC treatment leads to increased blastocyst apoptosis and DNA damage, decreased cell proliferation, and altered expression of pluripotency-related and apoptosis-related genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Tsunemitsu Isa, Tamas Somfai, Motoharu Oyadomari, Kyota Fusho, Shihei Touma, Naoto Suzuki, Hiroyuki Kaneko, Yoshito Katagiri, Kazuhiro Kikuchi
Summary: The present study demonstrates the feasibility of in vitro embryo production for breeding management and conservation of the indigenous pig breed, Agu.
ANIMAL SCIENCE JOURNAL
(2022)
Article
Agriculture, Dairy & Animal Science
Muhammad Rosyid Ridlo, Eui Hyun Kim, Anukul Taweechaipaisankul, Byeong Chun Lee, Geon A. Kim
Summary: Successful attenuation of ER stress signaling benefits in vitro embryonal development, with adiponectin treatment enhancing the developmental capacity of porcine embryos by reducing ER stress and apoptosis, improving cleavage rates, blastocyst formation rates, and total cell numbers.
Review
Agriculture, Dairy & Animal Science
Peter J. Hansen
Summary: The embryo produced by in vitro techniques has significant potential for genetic improvement and is a crucial component in various technologies. Overcoming technical obstacles and optimizing culture conditions are necessary for fully utilizing the opportunities offered by in vitro-produced embryos.
Review
Agriculture, Dairy & Animal Science
Peter J. Hansen
Summary: The embryo produced through in vitro oocyte maturation, fertilization, and embryonic development is a valuable resource for genetic improvement and has the potential to enhance female fertility and produce offspring with superior health and production ability. It is also essential in various technologies such as gene editing, somatic cell nuclear cloning, stem cell technologies, and in vitro gamete generation. However, implementation of an embryo transfer program faces technical obstacles that need to be overcome for cost-effective implementation. Therefore, research goals include increasing the supply of oocytes from genetically elite females, improving the proportion of transferrable embryos, enhancing pregnancy establishment after transfer, reducing pregnancy wastage, and optimizing postnatal phenotype through culture conditions.
Article
Reproductive Biology
Daniel Veraguas-Davila, Maria Francisca Cordero, Soledad Saez, Darling Saez-Ruiz, Alejandro Gonzalez, Fernando Saravia, Fidel Ovidio Castro, Lleretny Rodriguez-Alvarez
Summary: The study evaluated the effects of zona pellucida removal on the in vitro and in vivo development of domestic cat embryos. Results showed that removal of the zona pellucida negatively impacted gene expression and prevented successful implantation of the embryos.
Article
Reproductive Biology
Albert Strohmeyer, Claudia Strohmeyer, Judith Reischl, Stephan Wallenhorst, Wolfgang Holtz
Summary: Successful non-surgical transfer of embryos to the uterine body in pigs has been achieved, but it was found that the low pregnancy rates experienced may be due to the unsuitable conditions for embryo development in the uterus.
Article
Agriculture, Dairy & Animal Science
Tien Dat Nguyen, Honghui Li, Yiquan Zhuang, Bowei Chen, Keiji Kinoshita, Muhammad Ameen Jamal, Kaixiang Xu, Jianxiong Guo, Deling Jiao, Kumiko Tanabe, Yunfang Wei, Zhuo Li, Wenming Cheng, Yubo Qing, Hong-Ye Zhao, Hong-Jiang Wei
Summary: This study successfully established an Asian elephant's fibroblast cell lines and performed interspecies somatic cell nuclear transfer with porcine oocytes. The reconstructed embryos showed in vitro development but failed to develop in vivo. Further investigation is needed to understand the reasons behind the in vivo developmental failure.
ANIMAL BIOTECHNOLOGY
(2023)
Review
Obstetrics & Gynecology
Quinton S. Katler, Jennifer F. Kawwass, Bradley S. Hurst, Amy E. Sparks, David H. McCulloh, Ethan Wantman, James P. Toner
Summary: The practice of in vitro fertilization has undergone significant changes over the years, leading to a reduction in the rate of twin pregnancies. This improvement can be attributed to advancements in laboratory techniques, preimplantation genetic testing, and revised guidance on embryo transfer. Single-embryo transfer has become the standard of care, resulting in a dramatic decrease in multifetal pregnancies without compromising treatment effectiveness.
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Huaxing Zhao, Yazheng Dong, Yuxing Zhang, Xiao Wu, Xianjun Zhang, Yalin Liang, Yanan Li, Fang Zeng, Junsong Shi, Rong Zhou, Linjun Hong, Gengyuan Cai, Zhenfang Wu, Zicong Li
Summary: The addition of 20 ng/mL SDF1 during pig oocyte IVM improves the maturation rate of oocytes and the cleavage and blastocyst rates of resulting embryos. SDF1 supplementation decreases oxidative stress, enhances mitochondrial function, and influences gene expression related to apoptosis in pig oocytes.
Article
Medicine, General & Internal
Zhijie Hu, Danjun Li, Qiuju Chen, Weiran Chai, Qifeng Lyu, Renfei Cai, Yanping Kuang, Xuefeng Lu
Summary: In this large-sample retrospective study, the incidence of ectopic pregnancy (EP) in frozen embryo transfer (FET) cycles was found to be slightly lower than fresh embryo transfer (ET) cycles, with no significant difference. Among patients undergoing ET cycles, the long agonist protocol was associated with a significantly lower risk of EP compared to the GnRH antagonist protocol.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Agriculture, Dairy & Animal Science
Jordi Ribas-Maynou, Yentel Mateo-Otero, Marina Sanchez-Quijada, Sandra Recuero, Ariadna Delgado-Bermudez, Marc Llavanera, Marc Yeste
Summary: Sperm telomere length may serve as a biomarker for embryo development in pigs, as sperm with longer telomeres lead to higher rates of morulae and blastocysts.
Article
Medicine, General & Internal
Wenjing Zhou, Zhaojun Cheng, Chong Wang, Ying Feng
Summary: Sequential transplantation is a new mode of in vitro fertilization and embryo transfer. This study compared the clinical outcomes of sequential transplantation, embryo transplantation, and blastocyst transplantation in freeze-thaw embryo transfer cycles. The results showed that the sequential transplantation group had a significantly higher number of transplanted embryos than the other two groups, and the blastocyst transplantation group had the highest implantation rate. The sequential transplantation cycle can achieve a higher clinical pregnancy rate and minimize wastage of transplanted embryos, making it a viable choice for some patients in resuscitation transplantation cycles.
Article
Agriculture, Dairy & Animal Science
Akira Yajima, Hisashi Owada, Suguru Kobayashi, Natsumi Komatsu, Kazuaki Takehara, Maria Ito, Kazuhide Matsuda, Kan Sato, Hisao Itabashi, Satoshi Sugimura, Shuhei Kanda
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
(2017)
Article
Genetics & Heredity
Shoko Ieda, Tomonori Akai, Yoko Sakaguchi, Sumi Shimamura, Atsushi Sugawara, Masahiro Kaneda, Satoko Matoba, Masanori Kagota, Satoshi Sugimura, Hirotsune Kaijima
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2018)
Article
Agriculture, Dairy & Animal Science
Satoshi Sugimura, Tadayuki Yamanouchi, Maria Grazia Palmerini, Yutaka Hashiyada, Kei Imai, Robert B. Gilchrist
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2018)
Article
Multidisciplinary Sciences
Tatsuma Yao, Rie Suzuki, Natsuki Furuta, Yuka Suzuki, Kyoko Kabe, Mikiko Tokoro, Atsushi Sugawara, Akira Yajima, Tomohiro Nagasawa, Satoko Matoba, Kazuo Yamagata, Satoshi Sugimura
SCIENTIFIC REPORTS
(2018)
Article
Agriculture, Dairy & Animal Science
Jun Ito, Kanna Meguro, Kazuki Komatsu, Takuya Ohdaira, Rina Shoji, Takahisa Yamada, Satoshi Sugimura, Yohei Fujishima, Akifumi Nakata, Manabu Fukumoto, Tomisato Miura, Hideaki Yamashiro
ANIMAL REPRODUCTION SCIENCE
(2020)
Article
Agriculture, Dairy & Animal Science
A. O. Hendawy, M. Shirai, H. Takeya, S. Sugimura, S. Miyanari, S. Taniguchi, K. Sato
JOURNAL OF DAIRY SCIENCE
(2019)
Review
Agriculture, Dairy & Animal Science
Amin Omar Hendawy, Mostafa Sayed Khattab, Satoshi Sugimura, Kan Sato
Article
Reproductive Biology
Ryosuke Suzuki, Mai Okada, Hiroki Nagai, Junichi Kobayashi, Satoshi Sugimura
Summary: This study for the first time visualized pronuclei (PN) in delipidated bovine zygotes using time-lapse cinematography (TLC), showing that abnormalities in PN morphokinetics are associated with subsequent embryonic development. Observations suggest that PN morphokinetic abnormalities are linked to developmental competence, highlighting the potential of non-invasive visible light TLC by delipidation as a powerful tool for studying the relationship between PN morphokinetics and embryonic development.
Article
Biochemistry & Molecular Biology
Hiroki Nagai, Mai Okada, Yoko Nagai, Yoshiyuki Sakuraba, Hiroaki Okae, Ryosuke Suzuki, Satoshi Sugimura
Summary: Partial compaction is a self-correction mechanism observed in both human and bovine embryos to avoid aneuploid mosaicism. Excluded blastomeres in embryos are more likely to be aneuploid, while the remaining morulae show euploidy. Abnormal cleavages, such as reverse and direct cleavages, occur more frequently in the excluded blastomeres, suggesting their potential role in the self-correction mechanism during mammalian preimplantation embryo development.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Tatsuma Yao, Akane Ueda, Atchalalt Khurchabilig, Daisuke Mashiko, Mikiko Tokoro, Hiroki Nagai, Tei Sho, Satoko Matoba, Kazuo Yamagata, Satoshi Sugimura
Summary: In assisted reproductive technology (ART)-derived embryos of non-rodent mammals, chromosome segregation errors during early cleavage can have a significant impact on subsequent embryonic development. This study developed a non-invasive live-cell imaging technique to observe chromosome segregation dynamics in bovine preimplantation embryos. The results showed that abnormal chromosome segregation during early cleavage affected the formation of blastocysts, and embryos with severe chromosome segregation errors had prolonged cell cycle durations. The technique also had the potential to select viable ART-derived embryos for medical and livestock production.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Reproductive Biology
Ryosuke Suzuki, Tatsuma Yao, Mai Okada, Hiroki Nagai, Atchalalt Khurchabilig, Junichi Kobayashi, Kazuo Yamagata, Satoshi Sugimura
Summary: Abnormal fertilization, such as multi-PN and pronuclei migration error, is the primary cause of direct cleavage during the first mitosis. Observing direct cleavage helps exclude embryos with abnormal fertilization and improves the success rate of in vitro embryo transfer.
Article
Biotechnology & Applied Microbiology
Amin Omar Hendawy, Satoshi Sugimura, Kan Sato, Mohamed Mohsen Mansour, Ayman H. Abd El-Aziz, Haney Samir, Md. Aminul Islam, A. B. M. Rubayet Bostami, Ahmed S. Mandour, Ahmed Elfadadny, Rokaia F. Ragab, Hend A. Abdelmageed, Amira Mohammed Ali
Summary: Enzymes excreted by rumen microbiome help convert plant materials into nutrients required for animal growth. Factors such as diet, animal age, and health affect the structure of the rumen microbial community. The use of natural supplements, including selenium, has been explored to enhance rumen microbial nutrient supply. This review focuses on the effects of selenium on specific rumen microorganisms, fermentation, and digestibility. Positive effects on various parameters were observed, with selenium nanoparticles showing higher effectiveness. Further studies comparing synthetic selenium nanoparticles and probiotics enriched with sodium selenite are needed.
FERMENTATION-BASEL
(2022)
Article
Agriculture, Dairy & Animal Science
Kazuki Komatsu, Kohsuke Murata, Tsugumi Iwasaki, Syun Tokita, Shiina Yonekura, Satoshi Sugimura, Yohei Fujishima, Akifumi Nakata, Tomisato Miura, Hideaki Yamashiro
Summary: The study reveals that wild large Japanese field mice show dynamic changes in ovarian morphology and estradiol concentrations during the breeding season, indicating a correlation between seasonal follicle development and estradiol regulation. This species is considered as a potential alternative animal model for investigating seasonal reproductive changes and the impacts of environmental changes.
ANIMAL REPRODUCTION
(2021)
Article
Agriculture, Dairy & Animal Science
Satoshi Sugimura, Dulama Richani, Robert B. Gilchrist
ANIMAL REPRODUCTION
(2018)
Article
Agriculture, Dairy & Animal Science
Satoshi Sugimura, Tomonori Akai, Kei Imai
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2017)