Journal
PHYTOCHEMISTRY
Volume 117, Issue -, Pages 34-42Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2015.05.021
Keywords
Flammulina velutipes; Basidiomycetes; Betaine lipid; Glycerolipid metabolism; Phosphate starvation; Gene expression; BTA1; CHO2; CPT1; Phylogenetic analysis
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Funding
- Komarov Botanical Institute of the Russian Academy of Sciences [01201255617]
- Russian Foundation for Basic Research Grants [14-04-01795, 15-04-06211]
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Diacylglyceryltrimethylhomoserines (DGTS) are betaine-type lipids that are phosphate-free analogs of phosphatidylcholines (PC). DGTS are abundant in some bacteria, algae, primitive vascular plants and fungi. In this study, we report inorganic phosphate (P-i) deficiency-induced DGTS synthesis in the basidial fungus Flammulina velutipes (Curt.: Fr.) Sing. We present results of an expression analysis of the BTA1 gene that codes for betaine lipid synthase and two genes of PC biosynthesis (CHO2 and CPT1) during phosphate starvation of F. velutipes culture. We demonstrate that FvBTA1 gene has increased transcript abundance under phosphate starvation. Despite depletion in PC, both CHO2 and CPT1 were determined to have increased expression. We also describe the deduced amino acid sequence and genomic structure of the BTA1 gene in F. velutipes. Phylogenetic relationships between putative orthologs of BTA1 proteins of basidiomycete fungi are discussed. (C) 2015 Elsevier Ltd. All rights reserved.
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