Article
Engineering, Biomedical
Awuniji Linus, Mohammadhossein Ebrahimi, Mikael J. Turunen, Simo Saarakkala, Antti Joukainen, Heikki Kroger, Arto Koistinen, Mikko A. J. Finnila, Isaac O. Afara, Mika E. Mononen, Petri Tanska, Rami K. Korhonen
Summary: High-resolution Fourier transform infrared microspectroscopy (HR-FTIR-MS) was used to characterize collagen content in the chondrocyte microenvironment, revealing collagen loss in the pericellular region in early osteoarthritis before extending to the extracellular matrix in advanced stages. This technique may facilitate the discovery of new mechanisms and improve treatment strategies for osteoarthritis.
ACTA BIOMATERIALIA
(2021)
Article
Chemistry, Multidisciplinary
Samir Rosas, Keegan A. Schoeller, Edward Chang, Hongyan Mei, Mikhail A. Kats, Kevin W. Eliceiri, Xinyu Zhao, Filiz Yesilkoy
Summary: Label-free and nondestructive mid-infrared vibrational hyperspectral imaging is an important tissue analysis tool that provides spatially resolved biochemical information. This study introduces an advanced mid-infrared spectrochemical tissue imaging modality using metasurfaces to capture quantitative molecular maps of large-area murine brain tissue sections. The results demonstrate that plasmonic metasurfaces enhance chemical contrast in infrared images and enable the detection of ultrathin tissue regions. This imaging method has the potential to have a broad impact on translational research and clinical histopathology.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Analytical
Mei Li, Lu Zhang, Ling-Li Jiang, Zhi-Bo Zhao, You-Hua Long, Dong-Mei Chen, Jun Bin, Chao Kang, Ya-Juan Liu
Summary: This study proposed a label-free, high-throughput imaging method for cellular investigation of apple fruit ring rot infected by Botryosphaeria dothidea. By using confocal Raman microspectroscopic imaging technology combined with multivariate curve resolution-alternating least squares algorithm (MCR-ALS), accurate and pure molecular imaging was achieved. Additionally, a rapid and non-destructive classification method was developed using a fiber-optic probe near-infrared reflection spectrometer in conjunction with machine learning.
Article
Spectroscopy
Despina Moissidou, Hayley Derricott, Gihan Kamel
Summary: This case report details the examination of the skin of an Egyptian mummified head with a possible skin disorder, dating back to the New Kingdom. The study used Synchrotron Radiation Fourier Transform Microspectroscopy to reveal biochemical modifications in the mummified skin indicative of tissue degradation, as confirmed by comparing it to modern tissue degradation.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Thulya Chakkumpulakkal Puthan Veettil, Diana Alves, Jitraporn Vongsvivut, Rosemary L. Sparrow, Bayden R. Wood, Gil Garnier
Summary: Oxidative treatment of RBCs prior to freeze-drying stabilizes the cells for storage at room temperature. Synchrotron-based FTIR microspectroscopy was used to analyze single RBCs and compare lipid and protein spectral data before and after oxidation and freeze-drying. The analysis showed that oxidized and freeze-dried RBCs have different spectral profiles compared to untreated RBCs, indicating changes in lipid peroxidation, stiffness of the membrane, and protein secondary structure. Freeze-drying did not introduce additional changes. This study highlights the potential of freeze-dried RBCs as a stable source for blood serology testing and demonstrates the power of synchrotron FTIR microspectroscopic live-cell analysis.
Article
Multidisciplinary Sciences
Gareth O. S. Williams, Elvira Williams, Neil Finlayson, Ahmet T. Erdogan, Qiang Wang, Susan Fernandes, Ahsan R. Akram, Kev Dhaliwal, Robert K. Henderson, John M. Girkin, Mark Bradley
Summary: The use of optical techniques for rapid analysis and diagnostics has led to the development of new optical microscopy technologies. The high-speed optical scanning microscope can capture time-resolved images with detailed data cubes for studying diverse light-driven phenomena. Integration of system control electronics and on-chip processing overcomes challenges of high data volume and low imaging speed.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Marek Stiborek, Lenka Jindrichova, Stanislava Meliorisova, Antonin Bednarik, Vadym Prysiazhnyi, Jiri Kroupa, Pavel Houska, Barbora Adamova, Jarmila Navratilova, Viktor Kanicky, Jan Preisler
Summary: This study presents a new technique for digital mapping of biomarkers in tissues using infrared laser ablation and counting intact gold nanoparticle tags. The technique demonstrated successful mapping of a proliferation marker in three-dimensional aggregates of colorectal carcinoma cells, generating sharp distribution maps of the specific biomarker. The developed methodology shows high accuracy and potential applications with multielemental mass spectrometers.
ANALYTICAL CHEMISTRY
(2022)
Article
Optics
Lirong Qiu, Yunhao Su, Ke-Mi Xu, Han Cui, Dezhi Zheng, Yuanmin Zhu, Lin Li, Fang Li, Weiqian Zhao
Summary: A divided-aperture laser differential confocal 3D Geometry-Raman-Brillouin microscopic detection technology is proposed to simultaneously collect reflection, Raman, and Brillouin scattering signals in real time, allowing for the study of cell morphology and cancer development.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Chemistry, Analytical
Ryo Kato, Taka-aki Yano, Takeo Minamikawa, Takuo Tanaka
Summary: Visualizing the spatial distribution of chemical compositions in biological tissues is crucial for studying biological processes and diseases. In this study, we demonstrated the chemical imaging of heart tissues using mid-infrared photothermal microscopy, which provides high sensitivity and spatial resolution for observing different biomolecules.
ANALYTICAL SCIENCES
(2022)
Article
Pediatrics
Beverley Newman
Summary: Congenital lung malformations are often identified through prenatal US screening and may require further evaluation with fetal MRI. Most patients are asymptomatic at birth, leading to postnatal imaging being deferred until 3-6 months to decide on surgery or conservative management.
PEDIATRIC RADIOLOGY
(2022)
Article
Medicine, General & Internal
Federica Pederiva, Steven S. Rothenberg, Nigel Hall, Hanneke Ijsselstijn, Kenneth K. Y. Wong, Jan von der Thuesen, Pierluigi Ciet, Reuven Achiron, Adamo Pio d'Adamo, J. Marco Schnater
Summary: This article introduces the etiology, diagnosis, and treatment of congenital lung malformations (CLMs), and focuses on the impact of this disease on patients' quality of life.
NATURE REVIEWS DISEASE PRIMERS
(2023)
Article
Biochemistry & Molecular Biology
Heping Li, Tian Ning, Fan Yu, Yishen Chen, Baoping Zhang, Shuang Wang
Summary: The study confirmed that Raman spectroscopy combined with multivariate analysis technology has significant potential for improving the efficiency and performance of breast cancer diagnosis.
Article
Energy & Fuels
Kartika P. Savitri, Christoph Hecker, Freek D. van der Meer, Ridwan P. Sidik
Summary: Infrared spectroscopy (IRS) is used to identify minerals based on their spectral response to infrared light. While spot-based IRS is common in the geothermal industry, the introduction of infrared imaging spectroscopy (IRIS) shows potential for wider applications. The mineral exploration industry has embraced IRIS for years, and its development may lead to future trends in the geothermal industry.
Article
Optics
Bjorn Erik Haase, Joshua Hennig, Mirco Kutas, Erik Waller, Julian Hering, Georg von Freymann, Daniel Molter
Summary: Sensing with undetected photons in the infrared region is achieved through polarization-optics-based phase-quadrature implementation in a nonlinear interferometer. This allows for obtaining phase and visibility information with a single image acquisition without the need for phase variation, enabling the detection of dynamic processes. The method is demonstrated on both static and dynamic measurement tasks, showcasing its effectiveness.
Article
Chemistry, Analytical
Zhongyue Guo, Yeran Bai, Meng Zhang, Lu Lan, Ji-Xin Cheng
Summary: Antimicrobial resistance poses a major threat to global health and economics, necessitating rapid AST methods. We introduce a rapid AST method based on wide-field mid-infrared photothermal imaging, capable of metabolic imaging for hundreds of bacteria at single-cell resolution within seconds. This method enables probing of perturbed microbial protein synthesis within 1 hour after antibiotic treatment in E. coli cells, providing insights into antibiotic susceptibility.
ANALYTICAL CHEMISTRY
(2023)
Article
Surgery
Ciro Esposito, Lorenzo Masieri, Marco Castagnetti, Gloria Pelizzo, Mario De Gennaro, Gabriele Lisi, Giovanni Cobellis, Piergiorgio Gamba, Vincenzo Di Benedetto, Maria Escolino
Summary: This study confirmed the limited diffusion of robotic-assisted pediatric surgery in Italy. Pediatric surgeons need to obtain a certificate after virtual and experimental training before starting robotic surgery. The main indication for robotic surgery in children is reconstructive urological procedures.
JOURNAL OF LAPAROENDOSCOPIC & ADVANCED SURGICAL TECHNIQUES
(2023)
Article
Chemistry, Analytical
Tanveer Ahmed Shaik, Enrico Baria, Xinyue Wang, Florian Korinth, Joao L. Lagarto, Christiane Hoeppener, Francesco S. Pavone, Volker Deckert, Juergen Popp, Riccardo Cicchi, Christoph Krafft
Summary: This study investigated the biochemical, structural, and biomechanical effects of genipin (GE) cross-linking on equine pericardium (EP) using optical imaging techniques and atomic force microscopy (AFM). Results showed that GE cross-linking led to a decrease in second-harmonic signal and an increase in autofluorescence, indicating changes in biochemical and structural properties. Additionally, cross-linking increased the Young's modulus of EP.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Dana Cialla-May, Christoph Krafft, Petra Roesch, Tanja Deckert-Gaudig, Torsten Frosch, Izabella J. Jahn, Susanne Pahlow, Clara Stiebing, Tobias Meyer-Zedler, Thomas Bocklitz, Iwan Schie, Volker Deckert, Juergen Popp
ANALYTICAL CHEMISTRY
(2022)
Editorial Material
Pediatrics
Silvia Fattori, Alessandro Boscarelli, Daniela Codrich, Lydie Ammar, Egidio Barbi, Claudio Granata, Jurgen Schleef
JOURNAL OF PAEDIATRICS AND CHILD HEALTH
(2023)
Article
Chemistry, Applied
Silvia Pisani, Valeria Calcaterra, Stefania Croce, Rossella Dorati, Giovanna Bruni, Ida Genta, Antonia Avanzini, Marco Benazzo, Gloria Pelizzo, Bice Conti
Summary: This study aims to develop a shape memory engineered scaffold for improving the repair of myelomeningocele, the most common type of spina bifida defect, in fetuses. The engineered scaffold showed good shape memory properties and suitability for the proliferation of MSCs.
REACTIVE & FUNCTIONAL POLYMERS
(2022)
Article
Chemistry, Analytical
Karina Kocot, Katarzyna Pytlakowska, Ewa Talik, Christoph Krafft, Rafal Sitko
Summary: In this study, ss-cyclodextrin/graphene oxide (GO-ss-CD) was used as an effective adsorbent for dispersive micro-solid phase extraction (DMSPE) of uranyl ions (UO22+) from water samples. The adsorption capacity and resistance to high ionic strength make it a potential method for preconcentration of UO22+ ions. The accuracy of the method was verified by analyzing certified reference material and spiked water samples.
Article
Pediatrics
Mirco Cleva, Anna Vaivoda, Alessandro Boscarelli, Daniela Codrich, Anita Spezzacatene, Flora M. Murru
JOURNAL OF PAEDIATRICS AND CHILD HEALTH
(2023)
Article
Multidisciplinary Sciences
Julia Sophie Boeke, Juergen Popp, Christoph Krafft
Summary: This study utilized O-PTIR technology for the detection and identification of microplastic particles, analyzing the spectra of various plastic materials and introducing a novel two-dimensional identification method for better recognition of microplastic particles in environmental and biological samples.
SCIENTIFIC REPORTS
(2022)
Review
Optics
Christoph Krafft, Jurgen Popp
Summary: Modern optical and spectral technologies have the potential to characterize tissues at a molecular level, enabling the identification of pathological tissues and the grading and staging of tumors without the need for labeling. The current tools for intraoperative tumor assessment, as well as the requirements for tissue visualization from a medical and optical perspective, are described. Approaches utilizing optical and spectral techniques for ex vivo and in vivo monitoring are introduced, and proof-of-concept studies utilizing linear and nonlinear spectroscopy and imaging modalities are presented. The use of modern artificial intelligence and deep learning concepts to analyze spectroscopic and imaging datasets has contributed to the advancements in each technique. Finally, the article provides an outlook on the opportunities and prospects of clinical translation.
Article
Pediatrics
Alessandro Boscarelli, Emanuela Giglione, Maria Rita Caputo, Edoardo Guida, Marianna Iaquinto, Maria-Grazia Scarpa, Damiana Olenik, Daniela Codrich, Jurgen Schleef
Summary: This study examines evidence-based indications for robotic-assisted surgery (RAS) in pediatric surgery. The study found that the exclusive indications for RAS in pediatric population are pyeloplasty for ureteropelvic junction obstruction and ureteral reimplantation according to the Lich-Gregoire technique. Other indications for RAS in pediatric surgery are still under discussion and require further evidence.
TRANSLATIONAL PEDIATRICS
(2023)
Article
Oncology
Christoph Krafft, Juergen Popp, Peter Bronsert, Arkadiusz Miernik
Summary: Raman spectroscopy allows for label-free assessment of bladder tissue in intraoperative applications. In a retrospective study, Raman microspectroscopic images were used to identify different components of bladder tissue, including normal bladder, bladder cancer, necrosis, epithelium, and lipid inclusions. Additionally, foreign materials such as microplastic particles and pigments were detected in the specimens. This study demonstrates the potential of Raman spectroscopy for tumor detection and material identification in complex matrices.
Article
Surgery
Bianca Stefani, Daniela Codrich, Maria Rita Caputo, Edoardo Guida, Maria-Grazia Scarpa, Alessandro Boscarelli, Juergen Schleef
Summary: This study aimed to evaluate the quality of surgical informed consent and parents' late recall of surgical procedures and risks after pre-operative interview. The results showed that despite the perceived high quality of the informative materials and medical interview, parents' recall of surgical complications was poor.
FRONTIERS IN SURGERY
(2023)
Review
Pediatrics
Alessandro Boscarelli, Emanuela Giglione, Maria Rita Caputo, Edoardo Guida, Marianna Iaquinto, Maria-Grazia Scarpa, Damiana Olenik, Daniela Codrich, Jurgen Schleef
Summary: This study aims to evaluate the application of robotic-assisted surgery (RAS) in pediatric surgery based on published literature. A total of 10 articles with the highest level of evidence were analyzed after searching and screening databases. The results showed that there is clear evidence supporting the use of RAS in certain cases of urological surgery, while other applications in pediatric surgery still require further research.
TRANSLATIONAL PEDIATRICS
(2023)
Article
Chemistry, Analytical
Tanveer Ahmed Shaik, Anuradha Ramoji, Nils Milis, Juergen Popp, Christoph Krafft
Summary: This study demonstrates how the O-PTIR technique can overcome the limitations of direct mid-infrared spectroscopic imaging of single cells in an aqueous buffer. By using an indirect detection principle, the technique achieves high-resolution discrete wavenumber imaging and fingerprint spectroscopy of cultivated cells. The study also shows the potential applications of O-PTIR in high-content screening and subcellular analysis.
Article
Chemistry, Physical
Stefan Kranz, Markus Heyder, Stephan Mueller, Andre Guellmar, Christoph Krafft, Sandor Nietzsche, Caroline Tschirpke, Volker Herold, Bernd Sigusch, Markus Reise
Summary: The study investigated the effect of a zinc carbonate-hydroxyapatite-containing dentifrice on demineralized enamel and dentin surfaces. The results showed no significant differences in enamel surfaces, but a reduction in roughness and minor mineral precipitation on dentin surfaces.
