Article
Construction & Building Technology
M. Decker, J. Siegel, H. Hilbig, D. Heinz
Summary: This study investigates the ablation mechanisms of a frequently used 213 nm quintupled Nd:YAG ns laser on hardened cement paste and NIST glass, analyzing factors such as signal intensity and aerosol structures. It reveals a linear energy-signal behavior and similarities in aerosol formation with glass aerosols.
MATERIALS AND STRUCTURES
(2021)
Article
Optics
Jiuling Meng, Tao Lu, Bin Fang, Yang Ni, Bowen Fan
Summary: In this study, LA-ICP-MS was used to quantitatively analyze 46 trace elements in USGS samples. The results showed that the matrix effect had an impact on the analysis accuracy, with refractory elements being underestimated. Additionally, the laser sampling from different matrix samples directly determined the final analysis accuracy.
OPTICS AND LASER TECHNOLOGY
(2022)
Article
Energy & Fuels
Eliane V. Barros, Lindamara M. Souza, Nata C. L. Madeira, Luiz S. Chinelatto, Juliana N. Bertelli, Rogerio M. Carvalho, Boniek G. Vaz, Rosineide C. Simas, Alvaro C. Neto, Valdemar Lacerda, Wanderson Romao
Summary: This study presents an alternative method for the specific isolation of ARN from calcium naphthenate deposits, by cleaning and converting the salts, successfully separating the ARN species, supported by high-resolution mass spectrometry techniques.
Article
Chemistry, Analytical
Zita Salajkova, Marketa Hola, David Prochazka, Jakub Ondracek, David Pavlinak, Ladislav Celko, Filip Gregar, Petr Sperka, Pavel Porizka, Viktor Kanicky, Alessandro De Giacomo, Jozef Kaiser
Summary: This study demonstrates how sample surface topography significantly influences the laser ablation process and the analytical response of the LA-ICP-MS method. Different surface topographies can lead to misleading data interpretation, but roughening the sample surface can increase method sensitivity.
Article
Agriculture, Multidisciplinary
Hui Zhu, Katarzyna Bierla, Xueli Jin, Joanna Szpunar, Daijie Chen, Ryszard Lobinski
Summary: This study analyzed the Se metabolites of a Se-enriched probiotic and identified gamma-glutamyl-selenomethionine as the major Se metabolite. It also discovered 11 minor Se metabolites, 8 of which were not previously reported in probiotics. These findings demonstrate the potential of this probiotic as an alternative source of organic Se for food and feed supplementation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Polymer Science
Angela Villasenor, Raquel Sanchez, Marina Boccongelli, Jose-Luis Todoli
Summary: This study demonstrates the application of LA-ICP-MS for localized quantitative analysis of inclusions in polymeric industrial films, revealing that over 50% of the inclusions contain aggregates of multiple elements. Therefore, careful control of element distribution during production is necessary to avoid performance degradation.
Article
Biochemistry & Molecular Biology
Evgenii Drobyshev, Stefanie Raschke, Ronald A. Glabonjat, Julia Bornhorst, Franziska Ebert, Doris Kuehnelt, Tanja Schwerdtle
Summary: The study found that SeN has a slow transfer rate across the blood-brain barrier and is not metabolized by brain endothelial cells. Although there was evidence of SeN transfer on both sides of the barrier, speciation analysis indicated that PBCECs did not methylate SeN. Further research is needed to investigate the potential health-promoting effects of SeN in humans.
Article
Engineering, Environmental
Carsten Simon, Kai Duehrkop, Daniel Petras, Vanessa-Nina Roth, Sebastian Boecker, Pieter C. Dorrestein, Gerd Gleixner
Summary: Delta m matching can reveal structural information in complex mixtures and assess the quality of molecular formulas.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Anna Ruszczynska, Dorota Skrajnowska, Agata Jagielska, Barbara Bobrowska-Korczak, Barbara Wagner
Summary: Comparing tissues analyzed by LA-ICP-MS is challenging, but can be simplified by identifying regions of interest. In this study, two ROIs rich in fat or water were distinguished mathematically and analyzed chemically for targeted comparison.
APPLIED SCIENCES-BASEL
(2022)
Article
Food Science & Technology
Ruth Vanderschueren, Jasmien Doevenspeck, Florence Helsen, Sandra Mounicou, Jakob Santner, Jan A. Delcour, Eduardo Chavez, Erik Smolders
Summary: Previous studies have shown that the cadmium (Cd) concentration in cacao nibs decreases during fermentation, but only at a sufficiently low pH. However, this study found that acid amendments were ineffective in reducing the total Cd concentration in lab-scale experiments. Instead, decreasing the pH increased the water-extractable fraction of Cd. When embedded in a fermentation box, single pod derived beans showed a 1.25-fold decrease in Cd concentration after four days of fermentation. Visualization of the Cd gradient within beans revealed that fermentation enhances Cd mobility in the nibs.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Jing Jing Peng, Yang Liu, Fu Tian Yu, He Liang Fan, Shi Yang Yue, Yu Hui Fang, Xiao Ling Liu, Cheng-Hua Wang
Summary: A reliable method for simultaneous determination of four organic selenium species using HPLC-ICP-MS was developed and successfully applied in the analysis of selenium proteins in Lactococcus lactis. The method showed high sensitivity, good repeatability and accuracy, providing new insights and approaches for the identification and biosynthesis of organic selenium species.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemistry & Molecular Biology
Jordan Sonet, Anne-Laure Bulteau, Zahia Touat-Hamici, Maurine Mosca, Katarzyna Bierla, Sandra Mounicou, Ryszard Lobinski, Laurent Chavatte
Summary: Selenoproteins, which contain the rare amino acid selenocysteine with selenium atom, play crucial roles in cell homeostasis, antioxidant defense, and cell signaling in mammals. The expression of the selenoproteome, comprised of 25 selenoprotein genes, is tightly regulated by the selenium status of the body. The study presents an alternative strategy for characterizing selenoproteome regulation using radioactive Se-75 isotope and non-radioactive selenium isotopes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Katarzyna Bierla, Joanna Szpunar, Ryszard Lobinski, Roger A. Sunde
Summary: By using HPLC and ICP-MS, it has been found that selenium (Se) primarily exists as selenosugar in adequate turkey liver, while in rats, selenium is present as selenocysteine and selenosugar. Selenosugar-decorated proteins become the major Se-containing protein species in the liver with high Se supplementation.
Article
Biochemistry & Molecular Biology
Katarzyna Bierla, Joanna Szpunar, Ryszard Lobinski, Roger A. Sunde
Summary: This study found that the metabolism and accumulation of selenomethionine in rats is different from that of selenocysteine. At the selenium requirement for maximal glutathione peroxidase activity, selenocysteine is the major selenium species, while at higher intakes of selenomethionine, it is metabolized into low molecular weight selenosugars and selenosugar-decorated proteins. Additionally, compared to selenite, selenomethionine leads to less binding of inorganic selenium to liver proteins.
Article
Chemistry, Analytical
Raquel Gonzalez de Vega, Samantha Goyen, Thomas E. Lockwood, Philip A. Doble, Emma F. Camp, David Clases
Summary: This study investigates various strategies to enhance the detection and characterization of microplastics in complex matrices like seawater. It is found that ICP-MS/MS methods can improve size detection limits, reduce background signal, and increase selectivity.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Luluil Maknun, Katarzyna Kinska, Ivain Gonzalez-Alvarez, Laurent Ouerdane, Beiatrice Lauga, Atitaya Siripinyanond, Joanna Szpunar, Ryszard Lobinski
Summary: A method for quantifying iron-siderophore complexes by electrospray high-resolution accurate mass (HRAM) mass spectrometry without the use of authentic standards was developed. The complexes were purified by solid-phase extraction (SPE) and identified by fast size-exclusion chromatography (FastSEC)-Orbitrap MS(n) based on molecular mass and fragmentation. The ability of the complexes to exchange Fe-56 and Fe-58 was demonstrated by SEC with ICP MS and ESI MS detection. The method was applied to analyze peat samples from the French Pyrenean mountains and successfully identified and quantified 19 siderophores belonging to different classes.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Chemical
D. Kalampaliki, G. D. T. M. Jayasinghe, E. Avramiotis, I. D. Manariotis, D. Venieri, S. G. Poulopoulos, J. Szpunar, J. Vakros, D. Mantzavinos
Summary: In this study, valorized biomass was pyrolyzed and activated with potassium hydroxide to produce a biochar with a high specific surface area and significant amounts of surface oxygen species and carbonates. The activated biochar was used as an activator for sodium persulfate to induce the adsorption and oxidation of sulfamethoxazole in various water matrices. The degradation of sulfamethoxazole followed a pseudofirst order kinetic law, and the oxidation mechanism involved radical pathways and/or electron transfer.
CHEMICAL ENGINEERING RESEARCH & DESIGN
(2023)
Article
Biochemistry & Molecular Biology
Pawel Zagrodzki, Agnieszka Wiesner, Monika Marcinkowska, Marek Jamrozik, Enrique Dominguez-Alvarez, Katarzyna Bierla, Ryszard Lobinski, Joanna Szpunar, Jadwiga Handzlik, Agnieszka Galanty, Shela Gorinstein, Pawel Pasko
Summary: Due to selenium deficiency in humans, the search for new organic molecules containing this element in plant biofortification is important. This study evaluated selenium organic esters based on benzoselenoate scaffolds, with additional halogen atoms and various functional groups. The study aimed to discover the relationships between molecular characteristics of these compounds and the amount of sulfur phytochemicals in kale sprouts. The statistical model revealed a correlation structure between the molecular descriptors of selenium compounds and the biochemical features of the sprouts.
Article
Biochemistry & Molecular Biology
Caroline Vindry, Olivia Guillin, Philippe Wolff, Paul Marie, Franck Mortreux, Philippe E. Mangeot, Theophile Ohlmann, Laurent Chavatte
Summary: This study used CRISPR/Cas9 technology to engineer heterozygous and homozygous mutant human cells with a C65G mutation in tRNA([Ser]Sec). The mutation affected the expression level of tRNA([Ser]Sec), leading to altered selenoprotein expression and reduced sensitivity to selenium supplementation.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Fisheries
G. D. T. M. Jayasinghe, Joanna Szpunar, Ryszard Lobinski, E. M. R. K. B. Edirisinghe
Summary: A UPLC-MS/MS method was developed and validated for the quantitative confirmatory analysis of multi-class antibiotics residues in fish and shrimp. The method successfully detected 14 antibiotics belonging to different classes. The concentrations of antibiotics found in the samples complied with the EU regulation limits for veterinary drug residues in seafood and did not pose a risk in terms of food safety.
Article
Engineering, Environmental
Francisco Calderon Celis, Ivan Gonzalez-Alvarez, Magdalena Fabjanowicz, Simon Godin, Laurent Ouerdane, Beatrice Lauga, Ryszard Lobinski
Summary: Metallophores play a crucial role in metal scavenging and acquisition for many organisms. However, their identification in highly organic matter rich environments like peatlands is challenging due to low solubilization and metal scarcity. In this study, a novel two-dimensional solid-phase extraction-liquid chromatography-mass spectrometry (SPE-LC-MS) approach was developed for sensitive detection and identification of metallophores in native environments. By applying this method, 53 metallophores, including 36 bacterial ones, were identified in peat collected from a French peatland. The detection of characteristic metal isotope patterns also revealed potential complexes of iron and copper. Furthermore, a taxonomic-based inference method was implemented to associate the identified bacterial metallophores with potential producers, suggesting a new way to explore poorly documented environments for new metallophores and their producers.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Inorganic & Nuclear
Mikel Bernabeu de Maria, Diego Tesauro, Filippo Prencipe, Michele Saviano, Luigi Messori, Christine Enjalbal, Ryszard Lobinski, Luisa Ronga
Summary: Methylmercury, mercury (II), and mercury (I) chlorides were found to react with vasopressin and its mono- and diselenium analogues to form various mercury-peptide adducts. The replacement of Cys by SeCys in vasopressin increased reactivity towards methylmercury, resulting in the formation of -Se/S-Hg-Se-bridged structures and subsequent demethylation of methylmercury. Competitive experiments showed that CH3HgCl preferred to react with the diselenium analogue rather than vasopressin. The diselenium peptide also demonstrated the ability to displace CH3Hg moieties bound to S in vasopressin. These findings suggest the potential use of selenopeptides for methylmercury chelation and detoxification strategies.
INORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Olivia Guillin, Emmanuelle Albalat, Caroline Vindry, Elisabeth Errazuriz-Cerda, Theophile Ohlmann, Vincent Balter, Laurent Chavatte
Summary: This study investigated the impact of HIV-1 virus production on cellular zinc homeostasis and isotopic fractionation. The researchers found that HIV-1 captures a significant amount of cellular zinc in newly produced particles, and cells accumulate lighter zinc isotopes from the growth medium. The viruses and producing cells exhibit similar isotopic fractionation. The study also demonstrated that enriched isotopes can be used to trace the fate of zinc in HIV-1 infected cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Katarzyna Bierla, Simon Godin, Marta Ladanyi, Mihaly Dernovics, Joanna Szpunar
Summary: Automated and specific picking of selenium-containing molecular entities is achieved through a comprehensive pattern matching approach, and the reproducibility and selenium isotope selection are critically evaluated. Hydrophilic interaction liquid chromatography is used for one-step separation of water soluble metabolites, and absolute mass defect (MD) data are assessed by multivariate statistical tools for selenometabolite-specific screening.
Article
Biochemistry & Molecular Biology
Hui Zhu, Katarzyna Bierla, Jun Tan, Joanna Szpunar, Daijie Chen, Ryszard Lobinski
Summary: This study investigated the influence of the fermentation process on selenite metabolism and selenium enrichment in the probiotic Bifidobacterium longum DD98. The study evaluated the effects of sodium selenite concentration, feeding time, and fermentation stage on the distribution of selenium compounds and the speciation of selenium in the metabolite fraction. The results showed that the fermentation process significantly affected the incorporation of selenium into metabolites and proteins in B. longum DD98, offering a tool for optimizing the production of selenium-enriched probiotics with desired molecular speciation.
Article
Biochemistry & Molecular Biology
Katarzyna Bierla, Joanna Szpunar, Ryszard Lobinski, Roger A. Sunde
Summary: This study found that the metabolism and accumulation of selenomethionine in rats is different from that of selenocysteine. At the selenium requirement for maximal glutathione peroxidase activity, selenocysteine is the major selenium species, while at higher intakes of selenomethionine, it is metabolized into low molecular weight selenosugars and selenosugar-decorated proteins. Additionally, compared to selenite, selenomethionine leads to less binding of inorganic selenium to liver proteins.
Article
Chemistry, Analytical
Adam Sajnog, Katarzyna Bierla, Joanna Szpunar, Javier Jimenez-Lamana
Summary: This article discusses reliable quantification methods and sample preparation protocols for biogenic selenium nanoparticles. Ten different extraction approaches were compared and their efficiency was evaluated using single particle - ICP-MS. The results showed that enzymatic digestion resulted in the highest recoveries but also the broadest size distribution.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2023)
Article
Biochemistry & Molecular Biology
Katarzyna Bierla, Joanna Szpunar, Ryszard Lobinski, Roger A. Sunde
Summary: By using HPLC and ICP-MS, it has been found that selenium (Se) primarily exists as selenosugar in adequate turkey liver, while in rats, selenium is present as selenocysteine and selenosugar. Selenosugar-decorated proteins become the major Se-containing protein species in the liver with high Se supplementation.
Article
Chemistry, Analytical
Adam Sajnog, Katarzyna Bierla, Joanna Szpunar, Javier Jimenez-Lamana
Summary: The reliable quantification of biogenic SeNPs in complex biological matrices is a relatively new topic that has attracted attention from academia as well as the food and feed industry. Different extraction methods were compared, and it was found that enzymatic digestion resulted in the highest recoveries of SeNPs but also had the broadest size distribution.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2023)
Article
Chemistry, Multidisciplinary
Sergio Fernandez-Castillo Suarez, Cecile Courreges, Javier Jimenez-Lamana, Simon Godin, Sophie Nolivos, Regis Grimaud, Luisa Ronga, Joanna Szpunar, Joachim Allouche
Summary: The study of interaction mechanisms between nanomaterials and microbial cells at the nanoscale is crucial in various research fields. This paper demonstrates the biodegradation process of gelatin adsorbed on the surface of nanoparticles by Alteromonas macleodii extracellular proteases.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)
