Article
Cardiac & Cardiovascular Systems
Jie Su, Pierre Coleman, Angeliki Ntorla, Rhys Anderson, Michael J. Shattock, Joseph R. Burgoyne
Summary: This study demonstrates that the cytosolic DNA sensor cGAS can activate PKGI directly through the generation of cGAMP, showing a link between inflammation and blood pressure regulation.
Article
Biochemical Research Methods
Guido Domingo, Milena Marsoni, Luca Chiodaroli, Stefania Fortunato, Marcella Bracale, Maria Concetta De Pinto, Chris Gehring, Candida Vannini
Summary: 3',5'-cyclic adenosine monophosphate (cAMP) is recognized as a crucial signaling molecule in plants, and its role in cellular processes such as hormone response and environmental stimuli has been studied. A phosphoproteomic analysis of tobacco BY-2 cells with modulated cAMP levels revealed the significant impact of cAMP on plant kinome, mRNA processing, and cellular programming. The findings suggest the importance of unperturbed cellular cAMP levels for plant cellular homeostasis and signaling.
Article
Cell Biology
Aritra Bhadra, April K. Scruggs, Silas J. Leavesley, Naga Annamdevula, April H. George, Andrea L. Britain, Christopher M. Francis, Jennifer M. Knighten, Thomas C. Rich, Natalie N. Bauer
Summary: Second messenger signaling is essential for cellular processes. Our study demonstrates that cAMP-enriched EVs can induce second messenger signaling pathways, revealing the significance of EVs as a novel intercellular mechanism for cAMP signaling.
CELLULAR SIGNALLING
(2022)
Review
Cell Biology
Zhilei Wang, Nian Chen, Zhiyong Li, Guang Xu, Xiaoyan Zhan, Jianyuan Tang, Xiaohe Xiao, Zhaofang Bai
Summary: Inflammation is a protective response regulated by the host, with cGAS-STING pathway playing a crucial role in immune defense, viral infections, fatty liver, and cancer metastasis. The potential impact of this pathway in liver diseases has attracted widespread attention, with pharmacological agonists and antagonists offering new therapeutic possibilities. Understanding the mechanisms of cGAS-STING pathway may lead to the development of effective treatments for liver diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Plant Sciences
Linlin Qi, Jiri Friml
Summary: cAMP is a versatile second messenger in mammalian signaling pathways, but its role in plants is not well understood. Recent research has discovered that TIR1/AFB auxin receptors in plants have adenylate cyclase activity and are important for auxin signaling. This article summarizes the established cAMP signaling pathways in mammalian cells, discusses the turbulent history of plant cAMP research, and highlights the major progress and unresolved questions. The current paradigm of auxin signaling is also briefly reviewed to provide background for the discussion on the AC activity of TIR1/AFB auxin receptors and its potential role in transcriptional auxin signaling, as well as the impact of these discoveries on plant cAMP research in general.
Article
Immunology
Marina Wendlandt, Alina J. Kuerten, Antonia Beiersdorfer, Charlotte Schubert, Kiana Samad-Yazdtchi, Jessica Sauer, M. Carolina Pinto, Kristina Schulz, Manuel A. Friese, Christine E. Gee, Daniela Hirnet, Christian Lohr
Summary: This study found that ATP can stimulate the production of cAMP in astrocytes in the olfactory bulb of mice by acting on A(2A) receptors. The results showed no significant difference in this pathway between EAE mice and healthy mice.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Behavioral Sciences
Akihito Yasuoka, Kousuke Shimada, Masakatsu Nohara, Asuka Kamei, Fumika Shinozaki, Kaori Kondo, Ryo Inoue, Takashi Kondo, Keiko Abe
Summary: This study established a mouse model of weak depression and found that mice raised in single housing conditions showed reduced social interaction, learning disability, and lower plasma corticosterone levels. Microbiota analysis revealed significant differences in the cecal microbiota between the single housing group and the group housing group. Transcriptome analysis detected multiple differentially expressed genes in the amygdala and liver, which are implicated in the regulation of cyclic adenine monophosphate (cAMP) signaling and beta-oxidation. Interestingly, the expression levels of these genes showed a correlation with the occupancy of specific bacterial genera in the gut.
FRONTIERS IN BEHAVIORAL NEUROSCIENCE
(2022)
Article
Plant Sciences
Inas Al-Younis, Basem Moosa, Mateusz Kwiatkowski, Krzysztof Jaworski, Aloysius Wong, Chris Gehring
Summary: An amino acid search motif based on annotated ACs identified 12 unique Arabidopsis thaliana candidate ACs, with some involved in ABA biosynthesis. The study suggests the existence of "crypto-ACs" as low-activity ACs within multi-domain moonlighting proteins, potentially linking cAMP to ABA synthesis within complex plant proteins.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Otorhinolaryngology
Chuan Wang, Yuan Li, Wen Liu, Caiji Wang, Wen Jiang, Zeqi Zhao, Xuanyi Li, Yuehua Qiao, Chunfu Dai
Summary: This study aimed to detect the expression of Epac1 and Epac2 in the inner ear of guinea pigs and investigate their association with microcirculation in the inner ear. The results showed that both Epac1 and Epac2 were expressed in the inner ear and had significant expression differences in various tissues. In addition, immunofluorescence staining revealed their localization in different parts of the inner ear. These findings suggest that cyclic adenosine monophosphate-Epac1 signaling may play a crucial role in maintaining the blood-labyrinth barrier function and regulating microcirculation in the inner ear.
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Francesca Grisan, Martina Spacci, Carlotta Paoli, Andrea Costamagna, Marco Fantuz, Miriam Martini, Konstantinos Lefkimmiatis, Alessandro Carrer
Summary: This study demonstrates the impact of cholesterol on the plasticity of normal pancreatic acinar cells and the cyclic AMP/PKA axis. Cholesterol supplementation enhances ductal transdifferentiation in isolated primary wild-type acinar cells, associated with cAMP signaling. The ability of cholesterol to activate cAMP signaling is lost in tumor cells.
Article
Cell Biology
Lufei Wang, Lichi Han, Peng Xue, Xiangxiang Hu, Sing-Wai Wong, Meng Deng, Henry C. Tseng, Bo-Wen Huang, Ching-Chang Ko
Summary: The study found that dopamine inhibits osteoclast differentiation through the D2R/cAMP/PKA/CREB pathway. This reveals a potential mechanism for how the nervous system impacts bone remodeling and suggests future research directions for understanding bone development, repair, aging, and degenerative diseases.
CELLULAR SIGNALLING
(2021)
Article
Biochemistry & Molecular Biology
Feifei Wang, Gang Yu, Qing Yang, Xiao Yi, Linglin Fu, Yanbo Wang
Summary: In this study, a homogeneous polysaccharide was isolated from edible red seaweed and used as a biopolymer matrix to form composite films with small biological molecules. The addition of these molecules improved the barrier properties, wettability, mechanical strength, and antimicrobial activity of the films. The film composed of cAMP and the polysaccharide showed the best characteristics and was applied in fish packaging, extending the shelf life.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Biochemistry & Molecular Biology
Choel Kim, Rajesh Sharma
Summary: The interaction between the C-terminal catalytic domain and the N-terminal regulatory domain of cGMP-dependent protein kinases inhibits the intrinsic activity of the enzyme. Selective binding of cGMP to specific domains within the regulatory domain disrupts this inhibitory interaction, leading to activation of the enzyme. Crystal structures of different domains reveal specific contacts and conformational changes that explain the selectivity and activation of the enzyme by cyclic nucleotides.
Article
Microbiology
Ruizhen Li, Wenli Liu, Xiangrui Yin, Fangfang Zheng, Zhenyu Wang, Xingchen Wu, Xiaohua Zhang, Qian Du, Yong Huang, Dewen Tong
Summary: This study found that Brucella outer membrane protein Omp25 could suppress the production of IFN-beta and its downstream IFN-stimulated genes induced by various DNA viruses or IFN-stimulatory DNA. Omp25 promoted the degradation of cyclic GMP-AMP synthase (cGAS) via the ubiquitin-proteasome-dependent pathway, leading to the inhibition of IFN-beta induction in various mammalian monocyte/macrophages.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Aiguo Xia, Mingjie Qian, Congcong Wang, Yajia Huang, Zhi Liu, Lei Ni, Fan Jin
Summary: Researchers have developed an engineered Pseudomonas aeruginosa strain with light-dependent intracellular cAMP levels by introducing a photoactivated adenylate cyclase gene. Through blue light illumination, the engineered strain showed increased expression of cAMP responsive promoter and twitching activity, making it suitable for applications in controlling bacterial host infection. Additionally, directional twitching motility of the colonies was achieved through localized light illumination, which will aid in studying contact-dependent interactions between microbial species.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Ecology
Luisa Ederli, Gianandrea Salerno, Chantal Bianchet, Manuela Rebora, Silvana Piersanti, Stefania Pasqualini
ARTHROPOD-PLANT INTERACTIONS
(2020)
Article
Developmental Biology
Aloysius Wong, Lara Donaldson, Maria Teresa Portes, Jorg Eppinger, Jose A. Feijo, Christoph Gehring
Article
Biochemistry & Molecular Biology
Mateusz Kwiatkowski, Aloysius Wong, Anna Kozakiewicz, Christoph Gehring, Krzysztof Jaworski
Summary: This study uncovered the elusive cyclic nucleotide phosphodiesterases (PDEs) in plants and identified a potential PDE, Arabidopsis thaliana K+-Uptake Permease (AtKUP5). The PDE activity was confirmed through molecular docking analysis and was found to be stimulated by the Ca2+/CaM complex.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Biology
Ilona Turek, Chris Gehring, Helen Irving
Summary: Plant natriuretic peptides (PNPs) are hormonal peptides that affect plant responses to biotic and abiotic stress, and their interaction with proteins like rubisco activase (RCA) plays a role in regulating stress responses.
Editorial Material
Biochemistry & Molecular Biology
Aloysius Wong, Xuechen Tian, Yixin Yang, Christoph Gehring
Article
Plant Sciences
Luisa Ederli, Gianandrea Salerno, Mara Quaglia
Summary: Plants face challenges from multiple stress factors in ecosystems, with fungal pathogens influencing their defense responses against invasive pests. Different types of fungal pathogens trigger different hormonal pathways in plants, affecting their susceptibility to herbivores. The modulation of host volatile emission by necrotrophic fungi plays a role in plant defense against subsequent insect attacks, highlighting the intricate interactions between plants, microbes, and insects in nature.
JOURNAL OF PLANT RESEARCH
(2021)
Review
Plant Sciences
Aloysius Wong, Ningxin Hu, Xuechen Tian, Yixin Yang, Christoph Gehring
Summary: Reports of H-NOX-containing plant hemoproteins mediating important NO-dependent responses suggest the existence of NO sensing mechanisms in plants. These findings indicate a potential for uncovering more heme-based NO sensors that operate as regulatory sites, providing a more comprehensive understanding of plant responses to NO at both the molecular and systems level.
TRENDS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Claudius Marondedze, Giuliano Elia, Ludivine Thomas, Aloysius Wong, Chris Gehring
Summary: The study reveals that the proteome of Arabidopsis thaliana contains proteins with a specific citrullination signature, many of which have nucleotide-binding regulatory functions, and that changes in the citrullinome occur in response to cold stress. It also identifies an A. thaliana protein with peptidyl arginine deiminase activity that is calcium-dependent for many peptide substrates, highlighting the importance of this post-translational modification in cellular reprogramming during stress responses.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Mateusz Kwiatkowski, Aloysius Wong, Anna Kozakiewicz-Piekarz, Christoph Gehring, Krzysztof Jaworski
Summary: This passage discusses the roles of signaling molecules cAMP and cGMP, as well as Ca2+ in plants, and investigates the function of PDEs in monocot plants, identifying a candidate PDE from Brachypodium distachyon. The research shows that the PDE has a significant hydrolytic activity towards cAMP, which can be further enhanced by Ca2+ and CaM. Additionally, a comprehensive amino acid consensus sequence is constructed to aid in the identification of similar active sites in eukaryotes and prokaryotes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Inas Al-Younis, Basem Moosa, Mateusz Kwiatkowski, Krzysztof Jaworski, Aloysius Wong, Chris Gehring
Summary: An amino acid search motif based on annotated ACs identified 12 unique Arabidopsis thaliana candidate ACs, with some involved in ABA biosynthesis. The study suggests the existence of "crypto-ACs" as low-activity ACs within multi-domain moonlighting proteins, potentially linking cAMP to ABA synthesis within complex plant proteins.
FRONTIERS IN PLANT SCIENCE
(2021)
Editorial Material
Biochemistry & Molecular Biology
Aloysius Wong, Christoph Gehring
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Aloysius Wong, Chuyun Bi, Stefania Pasqualini, Chris Gehring
Summary: ABA is an ancient stress response molecule that affects various processes in plants and animals. Plant and animal proteomes may harbor undiscovered ABA-binding proteins in addition to known receptor complexes. By deducing amino acid search motifs from experimentally confirmed ABA-binding proteins, more candidates can be identified in the proteomes and, if validated, these candidates may have implications in immune responses and tumor progression among other processes.
PLANT GROWTH REGULATION
(2022)
Editorial Material
Biochemistry & Molecular Biology
Aloysius Wong, Xuechen Tian, Yixin Yang, Chris Gehring
Article
Biochemistry & Molecular Biology
Alma Costarelli, Chantal Bianchet, Luisa Ederli, Gianandrea Salerno, Silvana Piersanti, Manuela Rebora, Stefania Pasqualini
PLANT SIGNALING & BEHAVIOR
(2020)
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)
