Article
Cell Biology
Yayun Chen, Qian Jiang, Furong Qing, Junxia Xue, Qiuxiang Xiao, Wenji He, Lina Sui, Zhiping Liu
Summary: MDA5 enhances host susceptibility to invasive C. albicans infection by inducing cell apoptosis and compromising macrophage functions.
Article
Microbiology
Guisheng Zeng, Suat Peng Neo, Li Mei Pang, Jiaxin Gao, Shu Chen Chong, Jayantha Gunaratne, Yue Wang
Summary: This study established the first interactome of Cyr1 in Candida albicans and uncovered a potential role for cell wall proteins in directly regulating Cyr1 activity to determine growth forms in C. albicans.
MICROBIOLOGY SPECTRUM
(2022)
Editorial Material
Immunology
Damian J. Krysan
Summary: C. albicans undergoes morphological transition between yeast and hyphal forms, which is closely associated with its virulence. This transition also occurs after phagocytosis by macrophages. Multiple models have been proposed for the environmental factors in the phagolysosome that induce this transition, but none of them explain morphogenesis in macrophages, as discovered by H.B. Wilson and M.C. Lorenz.
INFECTION AND IMMUNITY
(2023)
Article
Biochemistry & Molecular Biology
Alessandro Vacchini, Elisa Maffioli, Dario Di Silvestre, Cinzia Cancellieri, Samantha Milanesi, Simona Nonnis, Sabrina Badanai, Pierluigi Mauri, Armando Negri, Massimo Locati, Gabriella Tedeschi, Elena Monica Borroni
Summary: This study reveals the differences in signaling properties between ACKR2 and its conventional counterpart CCR5 through quantitative phosphoproteomic analysis. The research found that ACKR2 may have roles other than its scavenger activity. This provides unprecedented details in chemokine receptor signaling and identifies potential targets for regulating ACKR2 and CCR5 function.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Immunology
Peng Gu, Ruofan Liu, Qin Yang, Li Xie, Rongjuan Wei, Jiaxin Li, Fengyi Mei, Tao Chen, Zhenhua Zeng, Yan He, Hongwei Zhou, Hongjuan Peng, Kutty Selva Nandakumar, Huikuan Chu, Yong Jiang, Wei Gong, Ye Chen, Bernd Schnabl, Peng Chen
Summary: The level of Candida albicans was decreased in bacterial sepsis patients, and the supernatant of Candida albicans culture showed bactericidal activity and improved sepsis symptoms. Phenylpyruvate (PPA) derived from Candida albicans enhanced the bactericidal activity of macrophages by binding to SIRT2 and increasing ROS production. PPA also correlated with the severity of sepsis in patients, highlighting the role of commensal fungi in bacterial disease modulation.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Mohsen Rashidi, Ali Bazi, Alireza Ahmadzadeh, Orazio Romeo, Ali Rezaei-Matehkolaei, Mahdi Abastabar, Iman Haghani, Sepideh Mirzaei
Summary: The present study investigated the growth inhibitory and apoptotic effects of umbelliprenin against Candida albicans in a BALB/c mice model of disseminated candidiasis. Umbelliprenin was able to eradicate fungal infection in BALB/c mice and promote antifungal effects, partly via inducing apoptosis and inhibiting the expression of enzymes and genes involved in antifungal resistance and adhesins encoding genes.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Review
Microbiology
Dalang Yu, Zhiping Liu
Summary: Candida albicans is an opportunistic pathogenic fungus that tends to infect hosts with impaired immune function, particularly cancer patients. Studies have shown that C. albicans infection increases the host's susceptibility to cancers such as oral, gastric, and colorectal cancer. This article aims to elaborate on the interaction between C. albicans and cancer development, and summarize potential molecular mechanisms, providing a theoretical basis for cancer prevention, diagnosis, and treatment.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Xu Wang, Shuangshaung Wu, Wenjie Wu, Wenqing Zhang, Linman Li, Qian Liu, Zhimin Yan
Summary: This study found that Candida albicans infection can promote the occurrence and progression of oral cancer, and attract tumor-associated macrophages (TAMs) infiltration. Further analysis showed that the activation of the IL-17A/IL-17RA signaling pathway played an important role in excessive TAMs infiltration in the tumor inflammatory microenvironment caused by Candida infection.
Article
Chemistry, Multidisciplinary
Tania Lima, Stefan B. Gunnarsson, Elisabete Coelho, Dmitry Evtuguin, Alexandra Correia, Manuel A. Coimbra, Tommy Cedervall, Manuel Vilanova
Summary: This study developed a method using nanoparticles to modulate the deleterious inflammatory response caused by fungal infection. The results showed that β-Glc-PS nanoparticles can reduce the inflammatory response caused by C. albicans cells and decrease the production of reactive oxygen species. This provides a theoretical basis for the development of targeted therapy for fungal infections.
Article
Immunology
R. Diez-Orejas, L. Casarrubios, M. J. Feito, J. M. Rojo, M. Vallet-Regi, D. Arcos, M. T. Portoles
Summary: This study evaluates the impact of hollow mesoporous SiO2CaO nanospheres labeled with fluorescein isothiocyanate (FITC-NanoMBGs) on the function of peritoneal macrophages, specifically their ability to phagocytize Candida albicans. The results indicate that the uptake of these nanospheres does not alter macrophage function, and intracellular FITC-NanoMBGs transiently increase fungal phagocytosis by macrophages at MOI 1. As the intracellular fungus quantity increases, the intracellular FITC-NanoMBG content decreases in a MOI- and time-dependent manner, demonstrating that macrophages distinguish between inert material and live yeast during intracellular incorporation.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Heesu Kim, Dong Gun Lee
Summary: Naringin-induced mitochondrial damage and dysfunction lead to oxidative stress and apoptosis in Candida albicans.
Article
Microbiology
Monica Cicuendez, Laura Casarrubios, Maria Jose Feito, Iratxe Madarieta, Nerea Garcia-Urkia, Olatz Murua, Beatriz Olalde, Nerea Briz, Rosalia Diez-Orejas, Maria Teresa Portoles
Summary: The study focused on the effects of human and porcine decellularized adipose matrices (DAMs) on the interaction between macrophages and Candida albicans. Differences in macrophage activation and morphological changes were observed when cultured on different DAMs, highlighting the influence of the extracellular matrix on macrophage response to fungal pathogens. This work contributes to understanding how ECM components can affect macrophage metabolism, immune function, and response to the microenvironment during an infection scenario.
Article
Multidisciplinary Sciences
Robert Zarnowski, Andrea Noll, Marc G. Chevrette, Hiram Sanchez, Ryley Jones, Hanna Anhalt, Jen Fossen, Anna Jaromin, Cameron Currie, Jeniel E. Nett, Aaron Mitchell, David R. Andes
Summary: The fungal pathogen Candida albicans can form biofilms that protect it from drugs and the immune system by releasing extracellular vesicles (EVs) that promote extracellular matrix formation and resistance to antifungal drugs. The study identifies functions for numerous EV cargo proteins in biofilm matrix assembly, drug resistance, fungal cell adhesion, and dissemination. The findings suggest that EVs play a crucial role in coordinating biofilm development in C. albicans.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Chaerim Kim, Jae-Goo Kim, Ki-Young Kim
Summary: Although Candida albicans commonly colonizes on mucosal surfaces, it can cause candidiasis as an opportunistic infectious fungus. The emergence of resistant Candida strains and the toxicity of anti-fungal agents have led to the development of new potential anti-fungal agents. Sclareol, a labdane-type diterpene, has shown anti-Candida activity with a minimum inhibitory concentration of 50 mu g/mL in 24 h. It induces apoptosis-like cell death, suppresses biofilm and hyphal formation in C. albicans, and is considered as a novel anti-fungal agent and anti-virulence factor.
Article
Immunology
Pu Song, Ge Peng, Hainan Yue, Takasuke Ogawa, Shigaku Ikeda, Ko Okumura, Hideoki Ogawa, Francois Niyonsaba
Summary: This study investigated the effect of Candidalysin on mast cell (MC) activation and the underlying molecular mechanism. The results showed that Candidalysin enhanced MC degranulation, migration, and production of cytokines, eicosanoids, and reactive oxygen species. Additionally, Candidalysin increased the expression of Dectin-1, and inhibiting Dectin-1 reduced MC activation.
JOURNAL OF CLINICAL IMMUNOLOGY
(2022)
Article
Clinical Neurology
Laura de Boni, Aurelia Hays Watson, Ludovica Zaccagnini, Amber Wallis, Kristina Zhelcheska, Nora Kim, John Sanderson, Haiyang Jiang, Elodie Martin, Adam Cantlon, Matteo Rovere, Lei Liu, Marc Sylvester, Tammaryn Lashley, Ulf Dettmer, Zane Jaunmuktane, Tim Bartels
Summary: The equilibrium of alpha-synuclein conformations is destabilized in post-mortem human brain tissue of sporadic PD and DLB patients, showing disease-specific patterns. Destabilization of multimers leads to increased levels of pathological alpha-synuclein, while pharmacological stabilization leads to prion-like aggregation resistance. The destabilization of alpha-synuclein multimers may be caused by regional neuronal vulnerability and prion-like aggregation transmission.
ACTA NEUROPATHOLOGICA
(2022)
Article
Biochemistry & Molecular Biology
Alfonso Rodriguez-Ruiz, Doreen Braun, Simon Pflug, Alexander Brol, Marc Sylvester, Clemens Steegborn, Ulrich Schweizer
Summary: This study investigates the catalytic mechanisms of deiodinase isoenzymes and the roles of several conserved cysteines. The study found that Cys124 forms a disulfide bond with selenocysteine during catalysis and can be reduced by glutathione. Mutagenesis studies also support a shared proton-relay pathway between DIO1 and DIO3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Giorgio Oliviero, Sergey Kovalchuk, Adelina Rogowska-Wrzesinska, Veit Schwammle, Ole N. Jensen
Summary: In this study, the chromatin proteomes of various mouse organs were analyzed using quantitative proteomics. The results showed distinct and organ-specific temporal protein expression profiles and regulation at the post-translational level. The spleen exhibited a static chromatin proteome, reflecting its important role in physiological defense and immunity. These findings support the hypothesis that chromatin-associated proteins are involved in distinct and specific physiological functions in ageing organs.
Article
Psychiatry
Anne-Kathrin Gellner, Aileen Sitter, Michal Rackiewicz, Marc Sylvester, Alexandra Philipsen, Andreas Zimmer, Valentin Stein
Summary: Chronic stress is a leading cause of neuropsychiatric conditions, such as depression and motor retardation, as well as neurodegenerative disorders. Through experiments on mice, we found that susceptible and resilient phenotypes exhibit differences in symptom load and motor learning abilities, and stress affects spine density and dynamics in the motor cortex based on the stress phenotype.
TRANSLATIONAL PSYCHIATRY
(2022)
Article
Biochemistry & Molecular Biology
Kirstine S. Norregaard, Henrik J. Jurgensen, Signe Z. Ingvarsen, Signe S. Heltberg, Christina E. Hagensen, Henrik Gardsvoli, Daniel H. Madsen, Ole N. Jensen, Lars H. Engelholm, Niels Behrendt
Summary: The endocytic collagen receptor uPARAP plays a crucial role in regulating extracellular levels of Thrombospondin-1 (TSP-1) and TSP-2, two matricellular proteins. By internalizing TSP-1 and TSP-2 for lysosomal degradation, uPARAP contributes to the regulation of ECM remodeling and has downstream effects on bone biology, fibrosis, and cancer. These findings highlight the importance of uPARAP in physiological and pathological processes.
Article
Multidisciplinary Sciences
Kyowon Jeong, Masa Babovic, Vladimir Gorshkov, Jihyung Kim, Ole N. Jensen, Oliver Kohlbacher
Summary: The detailed analysis and structural characterization of proteoforms by top-down proteomics has gained a lot of interest. FLASHIda is an intelligent online data acquisition algorithm that improves proteoform identification rates in complex samples.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Sergio Alonso-Fernandez, Ignacio Arribas-Diez, Gemma Fernandez-Garcia, Nathaly Gonzalez-Quinonez, Ole N. Jensen, Angel Manteca
Summary: This article describes the expansion of the Streptomyces phosphoproteome using optimized immobilized zirconium (IV) affinity chromatography and mass spectrometry, resulting in the identification of many novel phosphoproteins. The study enhances our understanding of Streptomyces development and secondary metabolism regulation.
JOURNAL OF PROTEOMICS
(2022)
Article
Biochemical Research Methods
Masa Babovic, Pavel Shliaha, Sebastian Gibb, Ole N. Jensen
Summary: Therapeutic proteins are important drugs for treating human ailments, and their amino acid sequence variants can impact their efficacy and safety. Researchers utilized an automated approach to systematically assess different mass spectrometry conditions for sequence analysis of a recombinant protein, resulting in near-complete coverage of the protein's amino acid sequence.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemistry & Molecular Biology
Marta Ignasiak-Kciuk, Karolina Nowicka-Bauer, Marta Grzechowiak, Tina Ravnsborg, Kamil Frackowiak, Ole N. Jensen, Mariusz Jaskolski, Bronislaw Marciniak
Summary: This study investigates the sensitized photo-oxidation reaction using carboxybenzophenone (CB) as a sensitizer and a PR-10 protein (MtN13) as a quencher. It reveals the specific binding of CB in the protein's hydrophobic cavity and the amino acid residues creating adducts with the sensitizer. The study also elucidates the mechanisms of CB quenching and the influence of neighboring groups on the oxidation process.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Oncology
Mette Louise Trempenau, Mikkel Bruhn Schuster, Sachin Pundhir, Mafalda Araujo Pereira, Adrija Kalvisa, Marta Tapia, Jinyu Su, Ying Ge, Bauke de Boer, Alexander Balhuizen, Frederik Otzen Bagger, Pavel Shliaha, Patrycja Sroczynska, Julian Walfridsson, Kirsten Gronbaek, Kim Theilgaard-Moench, Ole N. N. Jensen, Kristian Helin, Bo T. T. Porse
Summary: This study identifies the Histone 3 Lysine 4 (H3K4) demethylase KDM5C as a tumor suppressor in acute myeloid leukemia (AML). Decreased KDM5C expression leads to increased cell growth in AML cell lines and mouse models. Mechanistically, KDM5C acts as a transcriptional repressor through its demethylase activity, resulting in upregulation of immature genes marked by H3K4me3. The association of KDM5C levels with long-term disease-free survival in female AML patients highlights the clinical relevance of this finding.
Meeting Abstract
Biochemistry & Molecular Biology
Karolina Nowicka-Bauer, Marta Ignasiak-Kciuk, Marta Grzechowiak, Tina Ravensborg, Kamil Frackowiak, Ole N. Jensen, Mariusz Jaskolski, Bronislaw Marciniak
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Chemistry, Analytical
Francis Berthias, Dale A. Cooper-Shepherd, Frederik H. V. Holck, James I. Langridge, Ole N. Jensen
Summary: The combination of cyclic ion mobility spectrometry (cIM) and electron capture dissociation (ECD) allows for the complete separation and sequencing of large isomeric peptides, providing a new method for identifying biologically important proteoforms in complex mixtures.
ANALYTICAL CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Vignesh Ramesh, Paradesi Naidu Gollavilli, Luisa Pinna, Mohammad Aarif Siddiqui, Adriana Martinez Turtos, Francesca Napoli, Yasmin Antonelli, Aldo Leal-Egana, Jesper Foged Havelund, Simon Toftholm Jakobsen, Elisa Le Boiteux, Marco Volante, Nils Joakim Faergeman, Ole N. Jensen, Rasmus Siersbaek, Kumar Somyajit, Paolo Ceppi
Summary: This study found an inverse association between short-chain fatty acids and EMT in non-small cell lung cancer patients. In vitro experiments showed that propionate treatment enhanced the epithelial transcriptional program and reduced the EMT phenotype in lung cancer cell lines. Animal experiments also confirmed that propionate can reduce lung cancer metastasis and lymph node spread. Further mechanistic investigation revealed that propionate treatment caused chromatin remodeling through p300-mediated histone acetylation.
EMBO MOLECULAR MEDICINE
(2023)
Article
Cell Biology
Vincent Gaggioli, Calvin S. Y. Lo, Nazaret Reveron-Gomez, Zuzana Jasencakova, Heura Domenech, Hong Nguyen, Simone Sidoli, Andrey Tvardovskiy, Sidrit Uruci, Johan A. Slotman, Yi Chai, Joao G. S. C. Souto Goncalves, Eleni Maria Manolika, Ole N. Jensen, David Wheeler, Sriram Sridharan, Sanjiban Chakrabarty, Jeroen Demmers, Roland Kanaar, Anja Groth, Nitika Taneja
Summary: Chromatin signalling cascade, regulated by a checkpoint, activates the histone methyltransferase EHMT2/G9a to assemble heterochromatin at stressed replication forks. G9a, together with SUV39h1, induces chromatin compaction through accumulating repressive modifications, H3K9me1/me2/me3, at stressed replication forks. Untimely heterochromatin disassembly by demethylase KDM3A exposes forks to PRIMPOL-mediated genome instability, leading to chemotherapeutic drug sensitivity.
NATURE CELL BIOLOGY
(2023)
Article
Food Science & Technology
Maria Eleni Mavrogeni, Mostafa Asadpoor, Jo H. Judernatz, Ingrid van Ark, Marc M. S. M. Wosten, Karin Strijbis, Roland J. Pieters, Gert Folkerts, Saskia Braber
Summary: This study establishes the antipathogenic and barrier-protective capacity of AOS and COS against C. difficile and its toxin, TcdA, while revealing their ability to promote TJ reassembly in Caco-2 cells.
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)
