Article
Biochemistry & Molecular Biology
Niannian Wang, Yinxia Zhang, Wei Li, Zhangchuan Peng, Huan Pan, Shan Li, Tingcai Cheng, Chun Liu
Summary: The pigment and structural color of insects are essential for body protection, ecological adaptation, and signal communication. This study identified the gene responsible for the Ursa mutant phenotype in the silkworm Bombyx mori as BmSoxD. The mutation in BmSoxD caused excessive melanin pigmentation in the larval epidermis by promoting the melanin metabolism pathway. These findings highlight the importance of BmSoxD in regulating melanin synthesis in insects.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Feifei Ren, Jiming Yan, Xiong Wang, Yukai Xie, Nan Guo, Luc Swevers, Jingchen Sun
Summary: In this study, RNA-seq analysis of the silkworm midgut after BmCPV1 infection identified 2520 differentially expressed genes (DEGs). Gene Ontology (GO) functional annotation showed that the DEGs predominantly functioned in binding, cell, and cellular processes. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotation revealed that the DEGs were mainly distributed in global and overview metabolism maps, translation, and signal transduction. Experimental validation confirmed that BmPGRP-S5 acts as a proviral host factor for BmCPV1 infection.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Xianghan Mei, Chun Li, Peilin Peng, Jue Wang, Enxi He, Zhiyong Qiu, Dingguo Xia, Qiaoling Zhao, Dongxu Shen
Summary: In this study, a C-type lectin gene called BmIML-2 was identified from the silkworm Bombyx mori. It was found that BmIML-2 can inhibit the proliferation of the BmNPV virus by facilitating cell apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
M. Zarske, J. Zagon, S. Schmolke, T. Seidler, A. Braeuning
Summary: Two real-time PCR methods were developed for specific detection of silkworm as an illegal ingredient in feed, using single- and multi-copy approaches. The single-copy method showed high specificity, while the multi-copy method exhibited sensitivities as low as 0.1 pg B. mori DNA. The sequencing primer pair for Lepidoptera was found to be suitable for rapid screening purposes.
Article
Chemistry, Multidisciplinary
Can Cao, Zongkai Lin, Xiaochen Liu, Yanyan Jia, Eduardo Saiz, Stephan E. Wolf, Hanoch Daniel Wagner, Lei Jiang, Qunfeng Cheng
Summary: Coating Bombyx mori silks with a thin layer of reduced graphene oxide (rGO) has been shown to significantly increase their tensile strength and toughness. This bioinspired approach provides a universal and facile strategy for functionalizing natural fibers.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Virology
Mudasir Gani, Sergei Senger, Satish Lokanath, Pawan Saini, Kamlesh Bali, Rakesh Gupta, Vankadara Sivaprasad, Johannes A. Jehle, Joerg T. Wennmann
Summary: The study revealed the distribution of Indian BmNPV and investigated their genetic composition through methods like gene sequencing and phylogenetic analysis. It found genetic variations among different isolates and identified some new closely related viral strains.
Article
Entomology
Kakeru Yokoi, Takuya Tsubota, Akiya Jouraku, Hideki Sezutsu, Hidemasa Bono
Summary: In this study, reference transcriptome data for the silkworm was established and detailed examination of gene expression in silkworm tissues was performed. The results contribute to understanding silkworm biology and promoting industrial applications.
Article
Biochemistry & Molecular Biology
Mengjie Gao, Xinglin Mei, Cong Li, Pengcheng Yu, Dongxu Shen, Qiaoling Zhao
Summary: In the silkworm, distinct expression levels of intrinsic genes, protease activity, and signaling pathways were observed during different growth and development stages. The mini mutant discovered from the normal silkworm strain was controlled by a single recessive gene and exhibited homozygous lethality. Transcriptome analysis of the mini mutant revealed differential gene expression mainly involved in metabolic pathways and ribosome biogenesis. These results provide new insights into the mechanism underlying the formation of mini mutants.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Virology
Guanping Chen, Yuedong Li, Xiangshuo Kong, Shudi Zhao, Jiale Li, Xiaofeng Wu
Summary: This study found that the expression of Bombyx mori HEXIM1 (BmHEXIM1) was significantly upregulated after BmNPV infection. By suppressing BmRelish-driven immune responses, BmHEXIM1 assisted in the immune escape of BmNPV. This research provides new insights into the mechanisms of immune escape of BmNPV and theoretical guidance for the breeding of BmNPV-resistant silkworm varieties.
Article
Biochemistry & Molecular Biology
Tian Li, Chen Xu, Jinzhi Xu, Jian Luo, Bin Yu, Xianzhi Meng, Chunfeng Li, Guoqing Pan, Zeyang Zhou
Summary: The organelle proteome of silkworm BmE cells was identified and annotated using various tools and databases, revealing highly conserved proteins with potentially important roles in insect physiology. The development of SilkOrganPDB provides a valuable resource for further understanding the functions and evolution of these proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Susumu Katsuma, Keisuke Shoji, Yutaka Suzuki, Masashi Iwanaga
Summary: The study reveals that Bombyx mori larval ovaries exhibit greater resistance to Bombyx mori latent virus (BmLV) compared to cultured cells, even though small RNA pathways are active in both environments. Small RNAs derived from BmLV were detected in the ovaries, suggesting a potential role in defense mechanisms. However, the resistance of larval ovaries against BmLV does not solely rely on RNA silencing pathways.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Min Zhu, Xing Zhang, Jun Pan, Hanxue Zhu, Ziyao Zhang, Zi Liang, Guangli Cao, Xiaolong Hu, Renyu Xue, Chengliang Gong
Summary: The study revealed that claudin-2 can promote the infection of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV), and interact with BmCPV during viral infection, providing new mechanistic insights related to BmCPV infection.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Entomology
Kohei Kakino, Hiroaki Mon, Takeru Ebihara, Masato Hino, Akitsu Masuda, Jae Man Lee, Takahiro Kusakabe
Summary: This study characterizes the gene expression patterns and function in the testis of Bombyx mori, revealing peculiar features in spermatogenesis. The transcriptome analysis identifies widespread gene expression and testis-specific genes in B. mori, and compares it with Drosophila melanogaster for a better understanding. The study fills a knowledge gap and provides a basis for future research in spermatogenesis.
Article
Biochemistry & Molecular Biology
Dan Liang, Rui Shu, Song Jiang, Mengjun Xu, Yangyang Cai, Hongwei Qin, Daobo Zhang, Mengwei Feng, Junshan Gao, Yan Meng
Summary: Tetrahydrobiopterin (BH4) is a vital coenzyme for various enzymes in animals. Deficiency of BH4 in silkworms affects multiple metabolic pathways such as melanin synthesis, tyrosine metabolism, and the immune system. Fertilized eggs transfer a large amount of BH4 from the mother to offspring, ensuring their normal development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Li Jiang, Tianchen Huang, Qiang Liu, Shanshan Zhong, Dongxu Shen, Anli Chen, Qiaoling Zhao
Summary: Studying the mechanisms of feeding habits on artificial diet for different silkworm varieties is necessary for industrial silkworm rearing, but research in this area is currently lacking. This study conducted transcriptome analysis on the F-2 generation of silkworm varieties and identified 2188 differential genes related to feeding habits. Genes involved in olfactory system, energy supply, and cell proliferation and differentiation were found to contribute to feeding habits. The results provide a reliable reference for studying the molecular mechanisms of feeding habits on artificial diet in silkworms.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2023)
Article
Biochemistry & Molecular Biology
Pingan Han, Xiaoping Lu, Fugui Mi, Jing Dong, Chunlei Xue, Jianke Li, Bin Han, Xiaoyu Zhang
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2016)
Article
Biochemical Research Methods
Xinmei Huo, Bin Wu, Mao Feng, Bin Han, Yu Fang, Yue Hao, Lifeng Meng, Abebe Jenberie Wubie, Pei Fan, Han Hu, Yuping Qi, Jianke Li
JOURNAL OF PROTEOME RESEARCH
(2016)
Article
Agriculture, Multidisciplinary
Jiaojiao Wang, Yuhong Zhang, Xing Qin, Lingyu Gao, Bin Han, Deqing Zhang, Jinyang Li, He Huang, Wei Zhang
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2017)
Article
Biochemical Research Methods
Bin Han, Yu Fang, Mao Feng, Han Hu, Yue Hao, Chuan Ma, Xinmei Huo, Lifeng Meng, Xufeng Zhang, Fan Wu, Jianke Li
JOURNAL OF PROTEOME RESEARCH
(2017)
Article
Biochemical Research Methods
Zewdu Ararso, Chuan Ma, Yuping Qi, Mao Feng, Bin Han, Han Hu, Lifeng Meng, Jianke Li
JOURNAL OF PROTEOME RESEARCH
(2018)
Article
Multidisciplinary Sciences
Gebreamlak Bezabih, Han Cheng, Bin Han, Mao Feng, Yu Xue, Han Hu, Jianke Li
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Fan Wu, Chuan Ma, Bin Han, Lifeng Meng, Han Hu, Yu Fang, Mao Feng, Xufeng Zhang, Olav Rueppell, Jianke Li
Article
Biochemistry & Molecular Biology
Fan Wu, Yilu Feng, Bin Han, Han Hu, Mao Feng, Lifeng Meng, Chuan Ma, Linsheng Yu, Jianke Li
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2019)
Article
Biochemical Research Methods
Xufeng Zhang, Han Hu, Bin Han, Qiaohong Wei, Lifeng Meng, Fan Wu, Yu Fang, Mao Feng, Chuan Ma, Olav Rueppell, Jianke Li
MOLECULAR & CELLULAR PROTEOMICS
(2020)
Article
Biochemistry & Molecular Biology
Mao Feng, Yu Fang, Chuan Ma, Xiangyuan Duan, Yanyan Zhang, Bin Han, Han Hu, Lifeng Meng, Fuyi Wang, Jianke Li
Summary: Royal jelly (RJ), a natural honeybee product, has strong antibacterial activities, with N-glycosylated major royal jelly protein 2 (N-MRJP2) inhibiting the growth of Paenibacillus larvae by disturbing cell wall biosynthesis, increasing membrane permeability, hindering respiration, restraining division, and inducing cell death. This suggests that RJ is critical for the immune defense of small larvae.
Article
Biology
Bin Han, Qiaohong Wei, Fan Wu, Han Hu, Chuan Ma, Lifeng Meng, Xufeng Zhang, Mao Feng, Yu Fang, Olav Rueppell, Jianke Li
Summary: Behavioral specialization is crucial for the success of social insects, and honeybee workers show different response thresholds to task-specific stimuli corresponding to their behavioral phenotypes. Tachykinin signaling plays a functional role in regulating the responsiveness of specialized honeybee workers to specific tasks, suggesting a potential mechanism for controlling behavior in animals.
Article
Agriculture, Multidisciplinary
Han Hu, Qiaohong Wei, Zhihua Sun, Xufeng Zhang, Chuan Ma, Mao Feng, Lifeng Meng, Jianke Li, Bin Han
Summary: The study found that an increase in storage temperature and time is associated with decreased antimicrobial properties and conformational changes in royal jelly (RJ) proteins. Major RJ protein 4 (MRJP4) was identified as a sensitive and reliable marker for measuring RJ quality changes. The development of a colloidal gold immunoassay strip for MRJP4 detection provides a quick and simple method for evaluating RJ freshness.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemical Research Methods
Lifeng Meng, Solomon Zewdu Altaye, Mao Feng, Han Hu, Bin Han, Jianke Li
Summary: The two honeybee species, A. mellifera and A. cerana, have evolved substantial differences in olfactory-driven behaviors and peripheral olfactory systems. Protein phosphorylation plays a fundamental role in regulating signal transduction in the antennal lobes, with pathways like mTOR, phagosome, and autophagy being enriched. A. cerana shows enhanced synaptic plasticity for processing complex floral odor clues in mountain areas, potentially driven by unique phosphoprotein expression and upregulation of key proteins in the mTOR pathway.
JOURNAL OF PROTEOMICS
(2022)
Article
Biology
Bin Han, Qiaohong Wei, Esmaeil Amiri, Han Hu, Lifeng Meng, Micheline K. Strand, David R. Tarpy, Shufa Xu, Jianke Li, Olav Rueppell
Summary: This study found that queen bees predictably and reversibly adjust egg size based on colony size in a series of experiments. The size of the queen's ovary changes in the opposite direction. These adjustments are not solely determined by egg-laying rate, but by the queen's perception of the colony size. Egg size plasticity is associated with changes in ovarian proteins related to energy metabolism, protein transport, and cytoskeleton. The small GTPase Rho1 is identified as a candidate regulator of egg size.
Article
Microbiology
Megan E. Damico, Olav Rueppell, Zack Shaffer, Bin Han, Kasie Raymann
Summary: The study compared the gut microbiome composition of honey bees with high royal jelly production and Italian honey bees, finding that environmental factors play a more significant role in shaping gut bacterial composition than bee stocks. Further research is needed to investigate the impact of relative abundance differences in taxa on honey bee health.
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)