Journal
JOURNAL OF PROTEOMICS
Volume 75, Issue 12, Pages 3760-3777Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jprot.2012.04.047
Keywords
Type 1 diabetes mellitus; 2D-DIGE; MALDI-TOF mass spectrometry; Reactive oxygen species; Hemopexin
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Funding
- Chiayi Christian Hospital [R101-1]
- National Science Council, Taiwan [NSC 99-2314-B-705-002-MY2, NSC 100-2311-B-007-005]
- Nano- and Micro-ElectroMechanical Systems-based Frontier Research on Cancer Mechanism, Diagnosis, and Treatment grant from National Tsing Hua University
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Type 1 diabetes mellitus (T1DM) is an insulin-dependent metabolic disease in the world and often occurs in children and adolescents. Recent advances in quantitative proteomics offer potential for the discovery of plasma proteins as biomarkers for tracking disease progression and for understanding the molecular mechanisms of diabetes. Comparative proteomic analysis of the plasma proteomes from T1DM cases and healthy donors with lysine- and cysteine-labeling 2D-DIGE combining MALDI-TOF/TOF mass spectrometry revealed that 39 identified T1DM-associated plasma proteins showed significant changes in protein expression including hemopexin, and 41 in thiol reactivity. Further study showed that hemopexin can be induced in numerous cell lines by increasing the glucose concentration in the medium. Interestingly, glucose-induced hemopexin expression can be reduced by reactive oxygen species (ROS) scavengers such as glutathione, implying that hemopexin expression is linked to glucose-induced oxidative stress. In conclusion, the current work has identified potential T1DM biomarkers and one of these, hemopexin, can be modulated by glucose through a ROS-dependent mechanism. (c) 2012 Elsevier B.V. All rights reserved.
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