Review
Biochemistry & Molecular Biology
Hongwei Song, Hongyan Ma, Junfeng Shi, Yongping Liu, Chengxia Kan, Ningning Hou, Jing Han, Xiaodong Sun, Hongyan Qiu
Summary: Diabetes is a multifactorial disorder that has serious consequences on mortality and disability due to complications resulting from nonenzymatic glycation. This review provides a comprehensive understanding of the molecular mechanisms and pathological consequences of nonenzymatic glycation in diabetes, as well as various strategies to prevent and control glycation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Michael Hellwig, Julia Nitschke, Thomas Henle
Summary: A new pathway in the Maillard reaction involves the second glycation event of N-epsilon-carboxymethyllysine (CML) leading to a new class of Amadori rearrangement products. Under specific conditions, CMFL can be converted to CM-Fur, and incubation of bovine serum albumin with glucose results in the formation of CMFL and fructosyllysine.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Linhui Feng, Heping Cui, Pusen Chen, Khizar Hayat, Xiaoming Zhang, Chi-Tang Ho
Summary: The efficient preparation method of the ribose-glutathione Amadori rearrangement product as a precursor of meaty flavor was studied. The oxidation and degradation of glutathione during the preparation process were observed. Vacuum dehydration was found to inhibit the oxidation and degradation reactions and improve the yield of the product.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Alvaro Gonzalez-Dominguez, Francisco Visiedo, Jesus Dominguez-Riscart, Ma Carmen Duran-Ruiz, Ana Saez-Benito, Alfonso M. Lechuga-Sancho, Rosa Maria Mateos
Summary: In children with obesity and insulin resistance, the activity of erythrocyte catalase is impaired due to increased S-nitrosation of the enzyme. Other post-translational modifications (PTMs) such as O-glycosylation, tyrosine nitration, and S-glutathionylation were also observed in erythrocyte catalase, but levels of O-glycosylation and S-glutathionylation were reduced in the ObIR+ group.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Jennifer E. E. Moises, Christof Regl, Arthur Hinterholzer, Christian G. G. Huber, Mario Schubert
Summary: Glycation is a non-enzymatic and spontaneous post-translational modification that needs to be carefully monitored. 2D NMR spectroscopy can be used to identify glucose-induced glycation products.
PHARMACEUTICAL RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Buyan Pan, Marie Shimogawa, Jun Zhao, Elizabeth Rhoades, Anna Kashina, E. James Petersson
Summary: In this study, a facile method for introducing a mimic of arginylation modification into a protein of interest is presented. The effects of this mimicking modification on protein aggregation and neuroprotection are investigated.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Biochemistry & Molecular Biology
Mengyao Tang, Sahir Kalim
Summary: Non-enzymatic post-translational modifications (nPTMs) of proteins have emerged as novel risk factors for various diseases. Protein carbamylation and glycation are common nPTMs and potential therapeutic targets. Reducing the burden of these modifications is a promising therapeutic approach.
MOLECULAR ASPECTS OF MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Pauline Defant, Christof Regl, Christian G. Huber, Mario Schubert
Summary: This article presents a study on glycation, a non-enzymatic modification that occurs when reducing sugars react with free amines in protein side chains. The researchers conducted a detailed NMR analysis to identify the correlation patterns of the Amadori product formed between proteins and maltose. The results demonstrate that NMR spectroscopy is an independent method for detecting maltose-based glycation, which can be validated with other methods.
JOURNAL OF BIOMOLECULAR NMR
(2023)
Review
Biochemistry & Molecular Biology
Surya Kannan, Serhiy Souchelnytskyi
Summary: This study reviews novel post-translational modifications (PTMs) of human serum albumin, including phosphorylation, glycation, methylation, carbonylation, and acetylation. The potential impact of these modifications on albumin functions is discussed, highlighting their importance in medical applications.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Rinkey Shahu, Dinesh Kumar, Ahmad Ali, Kanchanlata Tungare, Khalid Mashay Al-Anazi, Mohammad Abul Farah, Renitta Jobby, Pamela Jha
Summary: Stevia leaf extract reduces the formation of advanced glycation end products (AGEs) and protein aggregation. It also reverses DNA glycation damage and has an impact on cell viability and glucose uptake. Stevia leaf extract shows potential as an inhibitor of glycation and could be used for the treatment of related conditions.
Article
Biochemistry & Molecular Biology
Benedikt Graf von Armansperg, Franziska Koller, Nicola Gericke, Michael Hellwig, Pravin Kumar Ankush Jagtap, Ralf Heermann, Janosch Hennig, Thomas Henle, Juergen Lassak
Summary: Thermally processed foods contain compounds such as fructoselysine, which can be utilized by gut microbiota as a nutrient source. The metabolism of fructoselysine in E. coli is tightly controlled at the transcriptional level, with the global regulator CRP (CAP) and the alternative sigma factor sigma 32 (RpoH) playing key roles in promoter activation. Additionally, a transcriptional regulator FrlR has been identified as a specific repressor for fructoselysine-6-phosphate, highlighting the complex interplay between global and substrate-specific regulation for efficient utilization of unusual substrates in the human gut environment.
MOLECULAR MICROBIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Shibin Deng, Heping Cui, Khizar Hayat, Shahzad Hussain, Muhammad Usman Tahir, Yun Zhai, Qiang Zhang, Xiaoming Zhang, Chi-Tang Ho
Summary: Additional DL-methionine did not affect the degradation rate of the methionine-glucose-derived Amadori rearrangement product, but played a role in subsequent reactions, resulting in a decrease in C-6-alpha-dicarbonyl compounds and facilitating the formation of glyoxal and methylglyoxal. The effect of Met addition was dependent on reaction temperature and contributed to the acceleration of browning formation by improving the formation of color precursors glyoxal and methylglyoxal.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Fahaad Alenazi, Mohd Saleem, Azharuddin Sajid Syed Khaja, Mubashir Zafar, Mohammed Salem Alharbi, Turki Al Hagbani, Mohd Yasir Khan, Saheem Ahmad
Summary: This study analyzed the effects of deoxyribose-induced IgG glycation on IgG structure and AGEs formation. The experimental results confirmed the modification in IgG structure and the formation of AGEs, which may contribute to secondary complications of diabetes.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Naoya Kitamura, James J. Galligan
Summary: Post-translational modifications (PTMs) rapidly respond to stimuli, finely tuning metabolism and gene expression to maintain homeostasis. Advances in mass spectrometry have expanded the list of known PTMs, but many lack defined mechanisms for their regulation. This review discusses the current state of PTM research, including site specificity, analytical methods, chemical tools, and approaches for discovering and validating novel PTMs, providing a comprehensive list and knowledge of their regulation and metabolic origins.
BIOCHEMICAL JOURNAL
(2023)
Article
Medicine, General & Internal
Binish Arif, Zubair Ahmad, Gulzar Ahmad Bhat, Syed Mudassar, Khursheed Aalam
Summary: This study analyzed the biophysical characterization of Amadori modified human serum albumin as a prognostic biomarker for diabetic complications. The results suggest that inhibiting the Amadori modification of albumin may be a logical approach in the prevention of diabetic complications.
JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH
(2022)
Article
Nutrition & Dietetics
Maria del Pilar del Pozo, Virginia Lope, Inmaculada Criado-Navarro, Roberto Pastor-Barriuso, Nerea Fernandez de Larrea, Emma Ruiz, Adela Castello, Pilar Lucas, Angeles Sierra, Isabelle Romieu, Veronique Chajes, Feliciano Priego-Capote, Beatriz Perez-Gomez, Marina Pollan
Article
Chemistry, Applied
H. Miho, J. Moral, D. Barranco, C. A. Ledesma-Escobar, F. Priego-Capote, C. M. Diez
Summary: In this study, 44 olive tree cultivars were evaluated for the phenolic profile of virgin olive oil (VOO), revealing significant variations in total phenol concentration among cultivars with the 'cultivar' factor contributing the most to the variance. The presence of three groups of cultivars with distinct phenolic profiles highlights the importance of considering phenolic composition in olive breeding programs.
Article
Chemistry, Analytical
L. S. Castillo-Peinado, M. Calderon-Santiago, F. Priego-Capote
Summary: The determination of vitamin D levels in human biological specimens is crucial due to its association with various biological disorders. Lyophilization of serum and plasma for sample storage prior to analysis does not affect sensitivity, precision, or accuracy, and can increase stability of target analytes.
Article
Agriculture, Multidisciplinary
Sonia Tome-Rodriguez, Carlos A. Ledesma-Escobar, Jose M. Penco-Valenzuela, Feliciano Priego-Capote
Summary: This study developed a method for qualitative and semi-quantitative determination of volatile components in thermally stressed virgin olive oils using HS-GC-MS. The volatile components were found to be closely correlated with the concentration of the main unsaturated fatty acids, explaining their impact on organoleptic properties.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Chemistry, Analytical
D. Luque-Cordoba, F. Priego-Capote
Summary: Steroidogenesis is a crucial process in maintaining physiological levels of steroids in the body, and its deficiency can lead to various pathologies. A research has developed a high-throughput and fully automated method for quantifying multiple steroids in human serum, achieving high sensitivity and selectivity. This method was tested on human serum samples and showed satisfactory results in line with physiological ranges reported in literature.
Article
Chemistry, Analytical
Laura de Los Santos Castillo-Peinado, Monica Calderon-Santiago, Aura Dulcinea Herrera-Martinez, Soraya Leon-Idougourram, Maria Angeles Galvez-Moreno, Rafael Luis Sanchez-Cano, Roger Bouillon, Jose Manuel Quesada-Gomez, Feliciano Priego-Capote
Summary: This study developed a method for measuring vitamin D-3 metabolism by analyzing multiple metabolites in human plasma, providing a comprehensive view of vitamin D-3 metabolism. The method was used to investigate metabolic correlations and differences between obese patients and healthy volunteers.
Article
Chemistry, Analytical
Laura de los Santos Castillo-Peinado, Monica Calderon-Santiago, Rafael Luis Sanchez-Cano, Jose Manuel Quesada-Gomez, Roger Bouillon, Feliciano Priego-Capote
Summary: Experts define vitamin D deficiency levels by the determination of a circulating 25-hydroxyvitamin D-3-calcifediol prohormone. Despite individuals not being affected by any disorder, a large part of the population is characterized by deficient vitamin D levels. This study proposes a quantitative method for the combined determination of phase I and phase II vitamin D-3 metabolites in human serum using tandem mass spectrometry detection.
Article
Chemistry, Analytical
Enrique C. Cabanas-Garrido, Carlos A. Ledesma-Escobar, Feliciano Priego-Capote
Summary: In the SARS-COV-2 pandemic, the use of surgical masks has been found to effectively reduce the transmission rate of the virus. Researchers have developed a method to analyze volatile organic compounds (VOCs) retained in surgical masks and identified 63 compounds associated with various chemical families. The study suggests that surgical masks can serve as a simple and inexpensive sampling system for VOC analysis.
Article
Biochemistry & Molecular Biology
Alejandro Hinojosa-Moscoso, Anna Motger-Alberti, Elena De la Calle-Vargas, Marian Marti-Navas, Carles Biarnes, Maria Arnoriaga-Rodriguez, Gerard Blasco, Josep Puig, Diego Luque-Cordoba, Feliciano Priego-Capote, Jose Maria Moreno-Navarrete, Jose Manuel Fernandez-Real
Summary: Excess iron is associated with dysfunction in adipose tissue and insulin resistance. In this study, it was found that markers of iron status were linked to changes in abdominal adipose tissue over one year. These associations were mainly observed in women and non-obese subjects, and were independent of insulin sensitivity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Carlos Augusto Ledesma-Escobar, Feliciano Priego-Capote, Monica Calderon-Santiago
Summary: MetaboMSDIA is a complete processing method for data-independent acquisition (DIA) files that extracts multiplexed MS2 spectra and identifies metabolites from open libraries. It improves the acquisition coverage and spectral quality in untargeted metabolomics, which are crucial for the tentative annotation of metabolites.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Applied
S. Tome-Rodriguez, F. Barba-Palomeque, C. A. Ledesma-Escobar, H. Miho, C. M. Diez, F. Priego-Capote
Summary: One of the nutritional benefits of olive oil is its high content of monounsaturated fatty acids (MUFAs). We studied the fatty acids composition of virgin olive oil samples obtained from different cultivars over multiple crop seasons. The cultivars were categorized into two groups based on their MUFAs content. We also found that the fatty acids profile can be influenced by the climate conditions, with reduced precipitation leading to a decrease in MUFAs and an increase in SFAs/PUFAs concentrations.
Article
Clinical Neurology
Laura S. Castillo-Peinado, Monica Calderon-Santiago, Bernabe Jurado-Gamez, Feliciano Priego-Capote
Summary: This study aimed to evaluate metabolic changes in sweat to aid in the diagnosis and assessment of cardiovascular consequences in obstructive sleep apnea (OSA). Permanent significant alterations in sweat metabolites were reported, and lactose, succinate, urea, and oxoproline were found to have significantly different effects on the severity of OSA.
JOURNAL OF SLEEP RESEARCH
(2023)
Article
Food Science & Technology
Ana Castillo-Luna, Hristofor Miho, Carlos A. Ledesma-Escobar, Feliciano Priego-Capote
Summary: Olive tree vegetal materials are a rich source of bioactive compounds, but their high moisture content hinders their preservation and extraction. Thus, a drying step is crucial for efficient extraction.
Article
Endocrinology & Metabolism
D. Luque-Cordoba, M. Calderon-Santiago, F. Priego-Capote
Summary: This study presents a strategy for identification and quantitative determination of acylcarnitines, metabolites involved in fatty acid beta-oxidation and organic acid metabolism, for evaluating metabolic disorders. The method combines data-independent acquisition and data-dependent acquisition techniques, achieving high accuracy and precision. The study also reveals significant differences in the acylcarnitines profiles between obese and non-obese individuals.
Article
Food Science & Technology
Inmaculada Criado-Navarro, Maria Asuncion Lopez-Bascon, Carlos Augusto Ledesma-Escobar, Feliciano Priego-Capote
Summary: The study found that using extra-virgin olive oil for deep-frying at high temperatures significantly reduces the content of phenolic compounds, leading to structural changes in these compounds.
ACS FOOD SCIENCE & TECHNOLOGY
(2021)