Article
Biochemistry & Molecular Biology
Natalia Lomova, Natalia Dolgushina, Alisa Tokareva, Vitaly Chagovets, Natalia Starodubtseva, Ilya Kulikov, Gennady Sukhikh, Vladimir Frankevich
Summary: This study aimed to investigate the lipid profile of follicular fluid in COVID-19 patients and evaluate its impact on the outcomes of in vitro fertilization (IVF) procedures, in order to identify potential biomarkers for prognosis. Mass spectrometry was used to analyze follicular fluid samples from 237 patients, revealing significant changes in several lipids. The results suggest that lipid metabolism in follicular fluid may be associated with pregnancy occurrence and outcomes after severe COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Caitlin R. Sacha, Irene Souter, Paige L. Williams, Jorge E. Chavarro, Jennifer Ford, Shruthi Mahalingaiah, Patricia K. Donahoe, Russ Hauser, David Pepin, Lidia Minguez-Alarcon
Summary: This study found a negative association between urinary concentrations of certain phthalate metabolites and follicular fluid anti-Mullerian hormone concentrations in women undergoing fertility treatment, which may have implications for antral follicle recruitment and fertility treatment outcomes.
ENVIRONMENT INTERNATIONAL
(2021)
Article
Agriculture, Dairy & Animal Science
Charley-Lea Pollard, Zamira Gibb, Jennifer Clulow, Agustin Ruiz, Alecia Sheridan, Mohammad Bahrami, Aleona Swegen, Christopher G. Grupen
Summary: This study aimed to investigate the effect of supplementing pregnant mares with nicotinic acid on the composition of NAD(+) metabolites in their blood and follicular fluid. The results demonstrate that supplementation of nicotinic acid increased the bioavailability of NAD(+) precursors in the follicular fluid of mares, potentially promoting the maturation of good quality oocytes.
Article
Obstetrics & Gynecology
Zohar Barnett-Itzhaki, Sarah Knapp, Chaya Avraham, Catherine Racowsky, Russ Hauser, Valentina Bollati, Andrea A. Baccarelli, Ronit Machtinger
Summary: The study found that phthalate metabolite concentrations are associated with the expression of extracellular vesicle microRNAs (EV-miRNAs) in follicular fluid, potentially impacting pathways related to oocyte development, maturation, and fertilization.
HUMAN REPRODUCTION
(2021)
Article
Endocrinology & Metabolism
Ying Ding, Yihong Jiang, Mingjiang Zhu, Qinling Zhu, Yaqiong He, Yao Lu, Yuan Wang, Jia Qi, Yifan Feng, Rong Huang, Huiyong Yin, Shengxian Li, Yun Sun
Summary: This study investigated the lipidomic profiles in follicular fluid (FF) of different phenotypes of Polycystic ovary syndrome (PCOS) and identified metabolic signatures and candidate biomarkers that provide a better understanding of the pathogenesis of PCOS.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Chemistry, Analytical
Ayako Takemori, Philipp T. Kaulich, Liam Cassidy, Nobuaki Takemori, Andreas Tholey
Summary: The combination of liquid chromatography (LC) and gas-phase separation by field-asymmetric ion mobility spectrometry (FAIMS) is a powerful proteoform separation system for top-down proteomics. In this study, an in-depth top-down proteomics workflow, GeLC-FAIMS-MS, was presented, which utilized a molecular-weight-based proteome fractionation approach using SDS-poly-acrylamide gel electrophoresis. The authors optimized the MS parameters for each gel band and compensating voltage (CV) to improve the identification of proteoforms. They also developed a simple and cost-effective method, combining the PEPPI protocol with an AnExSP method, for stain and SDS removal. Results showed that this method was better suited for low-molecular-weight proteoforms, while a methanol-chloroform-water (MCW)-based protocol showed advantages for higher-molecular-weight proteoforms.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Deseree J. Reid, Tapasyatanu Dash, Zhihan Wang, Craig A. Aspinwall, Michael T. Marty
Summary: Daptomycin is a cyclic lipopeptide antibiotic that interacts with lipid membranes in a non-specific manner. The interaction strength depends on membrane rigidity, with more rigid membranes showing stronger interactions. More fluid membranes may undergo pore formation to expose daptomycin to oxidation. These findings highlight the complementary use of native mass spectrometry, fast photochemical oxidation of peptides, and membrane conductance experiments in studying antibiotic-membrane interactions.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jiao Wu, Chunmei Liang, Xin Wang, Yida Huang, Wanshan Liu, Ruimin Wang, Jing Cao, Xun Su, Tao Yin, Xiaolei Wang, Zhikang Zhang, Lingchao Shen, Danyang Li, Weiwei Zou, Ji Wu, Lihua Qiu, Wen Di, Yunxia Cao, Dongmei Ji, Kun Qian
Summary: Metabolic fingerprinting of follicular fluid (MFFF) using particle-assisted laser desorption/ionization mass spectrometry (PALDI-MS) is performed to encode ovarian reserve (OR) and fertility. Machine learning analysis of MFFF allows for the diagnosis of diminished OR and identification of high-quality oocytes/embryos. Metabolic biomarkers from MFFF also contribute to fertility prediction in clinics.
Article
Endocrinology & Metabolism
Jianan Li, Zhourui Zhang, Yiqiu Wei, Pujia Zhu, Tailang Yin, Qiongqiong Wan
Summary: Ovarian reserve is crucial for female reproductive potential. Diminished ovarian reserve (DOR) leads to reduced number and quality of oocytes, and even with IVF-ET, the pregnancy and live birth rates are low. Metabolic analysis of follicular fluid revealed unique metabolic characteristics in DOR patients, providing insights into the pathogenesis and prediction of ovarian reserve function.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Reproductive Biology
J. A. Sauls-Hiesterman, K. E. Olagaray, S. E. Sivinski, B. J. Bradford, J. S. Stevenson
Summary: The study hypothesized that feeding a Saccharomyces cerevisiae fermentation product (SCFP) could facilitate early postpartum ovulation and affect metabolite and steroid hormone concentrations in Holstein cows. However, the results showed that SCFP had little effect on the metabolites and steroid hormones in blood and follicular fluid, except for androstenedione.
Review
Biochemistry & Molecular Biology
Michal Kaleta, Jana Oklestkova, Ondrej Novak, Miroslav Strnad
Summary: Neuroactive steroids are a family of compounds that can affect nervous system functions, synthesized in either the nervous system or peripheral endocrine tissues. Monitoring the production and levels of neuroactive steroids can be beneficial for the prevention, diagnosis, monitoring, and treatment of neurodegenerative and psychiatric diseases. Immunoanalytical methods and chromatography combined with mass spectrometry are highlighted as relevant techniques for analyzing neuroactive steroids.
Article
Chemistry, Multidisciplinary
Jiyeon Lee, Dahye Im, Yajie Liu, Jing Fang, Xibao Tian, Minsu Kim, Wen-Bin Zhang, Jongcheol Seo
Summary: A technique combining ion mobility spectrometry-mass spectrometry (IMS-MS) and supercharging electrospray ionization (ESI) has been demonstrated to effectively differentiate protein chemical topology. By incorporating as many charges as possible into proteins via supercharging ESI, the protein chains can be largely unfolded and stretched, revealing their hidden chemical topology. Different chemical topologies result in different geometric sizes of the unfolded proteins due to constraints in torsional rotations. The introduction of new topological indices allows for successful differentiation of various protein chemical topologies. This technique provides an easy classification of the topological features of complex proteins, previously concealed by their inherent complexity, and establishes a visual standard for discerning protein topologies.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Lu Li, Cuiji Sun, Yaoting Sun, Zhen Dong, Runxin Wu, Xiaoting Sun, Hanbin Zhang, Wenhao Jiang, Yan Zhou, Xufeng Cen, Shang Cai, Hongguang Xia, Yi Zhu, Tiannan Guo, Kiryl D. Piatkevich
Summary: This paper describes a spatially resolved proteomics method called Expansion Proteomics (ProteomEx), which combines tissue expansion with mass spectrometry-based proteomics. The method allows quantitative profiling of proteome variability in mammalian tissues using manual microsampling, without the need for custom equipment. The authors validated the method by analyzing brain, liver, and breast cancer samples, and demonstrated its utility by identifying proteins associated with Alzheimer's disease in a mouse model.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Roderick Corstiaan Abraham Versloot, Patricia Arias-Orozco, Matthijs Jonathan Tadema, Florian Leonardus Rudolfus Lucas, Xinghong Zhao, Siewert J. J. Marrink, Oscar Paul Kuipers, Giovanni Maglia
Summary: Mass spectrometry cannot distinguish between molecules with the same mass-to-charge ratio, but nanopore technology can differentiate diastereomeric and enantiomeric differences in peptides that cannot be observed by conventional MS analysis. The resolution in nanopore analysis is likely due to multiple chiral interactions during peptide transport across the nanopore, similar to chiral chromatography. Nanopore technology also offers possibilities for the rapid and cost-effective analysis of peptides, including those that cannot be easily differentiated by mass spectrometry.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Endocrinology & Metabolism
Fan He, Fang Wang, Yang Yang, Zhi Yuan, Chengguang Sun, Heng Zou, Huijia Chen, Hongliang Yi, Shan Hu Gao, Shen Zhang, Lina Hu, Ting-li Han
Summary: The co-treatment with GH during COS may improve oocyte development by altering FF metabolite profiles in DOR patients.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Obstetrics & Gynecology
Ragnheidur Valdimarsdottir, Anna-Karin Wikstrom, Theodora Kunovac Kallak, Evangelia Elenis, Ove Axelsson, Hubert Preissl, S. J. Kumari A. Ubhayasekera, Jonas Bergquist, Inger Sundstrom Poromaa
Summary: Women with PCOS have higher testosterone levels during pregnancy than women without PCOS. High maternal testosterone levels in early second trimester are associated with an increased risk of developing preeclampsia in women with PCOS.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Public, Environmental & Occupational Health
Oskar Karlsson, Joacim Rocklov, Alizee P. Lehoux, Jonas Bergquist, Anna Rutgersson, Martin J. Blunt, Linda S. Birnbaum
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY
(2021)
Article
Clinical Neurology
Valter Niemela, Anne-Marie Landtblom, Dag Nyholm, Maria Kneider, Radu Constantinescu, Martin Paucar, Per Svenningsson, Sandy Abujrais, Joachim Burman, Ganna Shevchenko, Jonas Bergquist, Jimmy Sundblom
Summary: Identifying molecular changes in Huntington's disease is crucial for therapy development. Mass-spectrometry analysis revealed differences in protein expression between HD patients and controls, with PENK levels potentially serving as a marker for the state of medium spiny neurons in HD.
MOVEMENT DISORDERS
(2021)
Article
Oncology
Ariadna Lara Gutierrez, Julia Hedlund Lindberg, Ganna Shevchenko, Inger Gustavsson, Jonas Bergquist, Ulf Gyllensten, Stefan Enroth
Summary: Cervical cancer is the fourth most common cancer among women worldwide, and screening programs increase its detection rate and survivability. Molecular screening for the presence of HPV as an alternative to physical examinations offers cost-efficient solutions, and our study found that protein biomarkers can aid in the detection of cervical cancer pre-stages.
Article
Biochemistry & Molecular Biology
Henrik Hill, Andris Elksnis, Per Lundkvist, Kumari Ubhayasekera, Jonas Bergquist, Bryndis Birnir, Per-Ola Carlsson, Daniel Espes
Summary: Gamma-aminobutyric acid (GABA) is an important inhibitory neurotransmitter found in the central nervous system and immune cells. Research has shown that GABA has immune-modulatory and beta-cell stimulatory effects and could potentially be used to treat type 1 diabetes. However, a study measuring the levels of GABA in T1D patients found that the levels were unchanged compared to healthy controls, suggesting that GABA secretion from beta-cells has minimal impact on systemic levels. The study also found correlations between GABA levels and certain cytokines and GAD-autoantibodies, indicating potential alterations in local GABA levels within pancreatic islets.
Article
Oncology
Yingya Zhao, Yu-Tang Gao, Xianglan Zhang, Alan L. Rockwood, Mark M. Kushnir, Qiuyin Cai, Jie Wu, Jiajun Shi, Qing Lan, Nathaniel Rothman, Yu Shyr, Xiao-Ou Shu, Wei Zheng, Gong Yang
Summary: The study suggests that higher levels of circulating free testosterone and estimated aromatase activity may be associated with lower lung cancer risk in postmenopausal never-smoking women.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Pharmacology & Pharmacy
Amol O. Bajaj, Mark M. Kushnir, Erik Kish-Trier, Rachel N. Law, Lauren M. Zuromski, Alejandro R. Molinelli, Gwendolyn A. McMillin, Kamisha L. Johnson-Davis
Summary: We developed an LC-MS/MS method for monitoring the concentrations of 6-TG and 6-MMP and evaluated the stability of thiopurine metabolites. The method showed good agreement with other methods and revealed differences in metabolite concentrations among different TPMT groups and age groups.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Medical Laboratory Technology
Mark M. Kushnir, Heather A. Nelson, Kelly Doyle
Summary: This review discusses the techniques used in the separation and direct quantification of FH concentrations in biological samples using mass spectrometry. Measurement of FH presents challenges, but equilibrium dialysis, ultrafiltration, or size-exclusion separation coupled with liquid chromatography-tandem mass spectrometry provide sensitive and specific methods for measurement.
JOURNAL OF APPLIED LABORATORY MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Wei Hu, Yaorui Hu, Yao Pei, Rongrong Li, Fuyi Xu, Xiaodong Chi, Jia Mi, Jonas Bergquist, Lu Lu, Luping Zhang, Chunhua Yang
Summary: A study found that there were 46 differentially expressed proteins between cervical carcinoma (CC) and normal tissues, with Integrin beta 6, PPP1CB, TMPO, PTGES3, and DTX3L significantly upregulated in CC, while Desmin was significantly downregulated. Silencing DTX3L could suppress CC cell proliferation, migration, invasion, and tumorigenesis, and enhance cell apoptosis. Furthermore, DTX3L silencing inhibited the activity of the PI3K/AKT/mTOR signaling pathway. These findings suggest that DTX3L may regulate CC progression through the PI3K/AKT/mTOR signaling pathway and could serve as a potential biomarker and therapeutic target for CC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Carmen Dunbar, Mark M. Kushnir, Yifei K. Yang
Summary: AFP serves as a serum biomarker for liver cirrhosis and hepatocellular carcinoma, with different glycosylation patterns distinguishing these conditions. Core fucosylated AFP-L3 is a marker for malignancy risk in HCC. A method combining immune enrichment, LC-HRMS, and protein analysis was developed to detect endogenous AFP proteoforms and quantify AFP-L3% in serum. The method showed reproducibility and agreement with a commercial assay, and identified AFP glycoforms without core fucosylation (AFPL1) and common AFP-L3 glycoforms (G2FS1 and G2FS2).
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Sofia Persson, Kumari A. Ubhayasekera, Jonas Bergquist, Sahruh Turkmen, Inger Sundstroem Poromaa, Evangelia Elenis
Summary: Polycystic ovary syndrome (PCOS) is a common endocrine disorder among women, and hyperandrogenism can cause psychological morbidity and impaired quality of life. This study aimed to investigate whether hyperandrogenism persists into midlife and its impact on quality of life.
Correction
Biochemical Research Methods
Carmen Dunbar, Mark M. Kushnir, Yifei K. Yang
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Food Science & Technology
Julio Cesar Espana Amortegui, Heidi Pekar, Mark Dennis Chico Retrato, Malin Persson, Bengt Karlson, Jonas Bergquist, Aida Zuberovic-Muratovic
Summary: A LC-MS/MS method was developed for the simultaneous identification and quantification of cyanotoxins in edible bivalves. The method successfully detected and resolved two previously detected cyanotoxin congeners separately. The method showed limitations for certain cyanotoxins but overall was specific and robust for the parameters investigated. The method was applied to analyze mussel and oyster samples collected along the Swedish coast, and the results can contribute to future regulatory monitoring for cyanotoxins to ensure seafood safety.
Article
Infectious Diseases
Anja Naas, Peng Li, Clas Ahlm, Elisabeth Aurelius, Josef D. Jarhult, Silvia Schliamser, Marie Studahl, Wenzhong Xiao, Jonas Bergquist, Gabriel Westman
Summary: This study investigated the temporal changes of the CSF proteome in patients with herpes simplex encephalitis (HSE) and its association with factors such as anti-NMDAR serostatus, corticosteroid treatment, brain MRI, and neurocognitive performance. The results showed that the composition and activation pathways of CSF proteins vary at different stages of the disease. Additionally, it was found that several proteins were less abundant in patients with anti-NMDAR seropositivity compared to seronegativity.
INFECTIOUS DISEASES
(2023)
Article
Chemistry, Analytical
Mark Dennis Chico Retrato, Siyuan Qiu, Anna Lundquist, Aida Zuberovic Muratovic, Farshid Mashayekhy Rad, S. J. Kumari A. Ubhayasekera, Jonas Bergquist
Summary: This study describes a simple and robust method for the identification and quantification of 22 fatty acids in TPN components using gas chromatography-mass spectrometry. The method was evaluated according to the guidelines from FDA, EMA, and ICH, and demonstrated good methodological performance. The developed method has potential applications for quality assurance and control of TPN products.
ANALYTICAL METHODS
(2023)
