Journal
JOURNAL OF PROTEOME RESEARCH
Volume 11, Issue 11, Pages 5167-5185Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr300362g
Keywords
hollow fiber culture system; label-free quantitative proteomics; PARK7; cancer metastasis
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Funding
- Ministry of Education, Taiwan, RO.C. under the NCKU Project of Promoting Academic Excellence & Developing World Class Research Centers
- National Science Council [NSC99-2923-M-006-001-MY3, NSC101-2325-B-006-003, NSC100-2113-M-006-002-MY3]
- National Science Council, Taiwan
- Tissue Bank, Research Center of Clinical Medicine, National Cheng Kung University Hospital
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As the leading cause of cancer death worldwide, lung cancer lacks effective diagnosis tools and treatments to prevent its metastasis. Fortunately, secretome has clinical usages as biomarkers and protein drugs. To discover the secretome that influences lung adenocarcinoma metastasis, the hollow fiber culture (HFC) system was used along with label free proteomics approach to analyze cell secretomes between CL1-0 and CL1-5 cell lines, which exhibit low and high metastatic potentials. Among the 703 proteins quantified, 50 possessed different levels between CL1-0 and CL1-5. PARK7 was a primary focus because of the lack of research involving lung adenocarcinoma. The cell proliferation, migration, and invasion properties of CL1-0, CL1-5, and A549 cells were significantly diminished when the expression of their PARK7 proteins was reduced. Conversely, these functions were promoted when PARK7 was overexpressed in CL1-0. In clinical expression, PARK7 levels within tissue specimens and plasma samples were significantly higher in the cancer group. This represents the first time the HFC system has been used with label free quantification to discern the elements of metastasis in lung adenocarcinoma cell secretomes. Likewise, PARK7 has never been researched for its role in promoting. lung adenocarcinorna progression.
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