Article
Veterinary Sciences
Jiao Hu, Peipei Peng, Jun Li, Qi Zhang, Rumeng Li, Xiaoquan Wang, Min Gu, Zenglei Hu, Shunlin Hu, Xiaowen Liu, Xinan Jiao, Daxin Peng, Xiufan Liu
Summary: The bivalent H5+H7 VLP vaccine candidate shows promising results in protecting poultry against highly pathogenic H5N1 and H7N9 avian influenza viruses, and may serve as a critical alternative to traditional egg-based inactivated vaccines.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Immunology
Vasilis C. Pliasas, Zach Menne, Virginia Aida, Ji-Hang Yin, Maria C. Naskou, Peter J. Neasham, J. Fletcher North, Dylan Wilson, Katharine A. Horzmann, Joshy Jacob, Ioanna Skountzou, Constantinos S. Kyriakis
Summary: This study evaluated the immunogenicity and efficacy of a novel vaccine against influenza virus infection in a pig model. The results showed that the vaccine induced high levels of anti-NA antibodies and provided protection comparable to a commercial vaccine.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Virology
Yu-Hsuan Yang, Ching-Hui Tai, Dayna Cheng, Ya-Fang Wang, Jen-Ren Wang
Summary: In this study, H5N6 virus-like particles (VLPs) were generated and tested for their potential as a broad-spectrum vaccine candidate against H5Nx avian influenza viruses. The results showed that H5N6 VLPs induced higher neutralizing antibody titers and increased production of immune-related cytokines compared to H5+N6 proteins, suggesting that H5N6 VLPs may be a potential vaccine candidate.
Article
Microbiology
Jun Li, Rumeng Li, Qi Zhang, Peipei Peng, Xiaoquan Wang, Min Gu, Zenglei Hu, Xinan Jiao, Daxin Peng, Jiao Hu, Xiufan Liu
Summary: This study demonstrates that the H7N9 VLP vaccine provides complete clinical protection against HP H7N9 virus, significantly inhibits virus shedding, and abolishes viral replication in chickens. The VLP vaccine represents a promising alternative strategy for the development of novel H7N9 avian influenza vaccines for chickens.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Weigang Ren, Shuli Pei, Wenming Jiang, Meixia Zhao, Le Jiang, Honggang Liu, Yongxiang Yi, Mizhou Hui, Junwei Li
Summary: A replication-deficient recombinant influenza virus was developed in this study, which stimulated robust immune response and conferred complete protection against H5N1 or H9N2 influenza virus infections in mice.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Kimberly Braz Gomes, Grace Lovia Allotey-Babington, Sucheta D'Sa, Sang-Moo Kang, Martin J. D'Souza
Summary: This study examined the activation status of dendritic cells (DCs) and the innate and adaptive immune responses after exposure to M2e VLP vaccine. Results showed that the M2e VLP microparticle had a strong stimulatory effect on DCs, and the presence of Alhydrogel(R) and MPL-A(R) adjuvants enhanced this effect.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Immunology
Mohamed H. El-Husseiny, Naglaa M. Hagag, Peter Pushko, Irina Tretyakova, Mahmoud M. Naguib, Abdel Satar Arafa
Summary: The study evaluated the efficacy of a VLP-based vaccine in SPF chickens, showing high levels of protection. VLPs could be a potential alternative vaccine candidate for the control of AIV infection in poultry.
Article
Biochemistry & Molecular Biology
Hae-Ji Kang, Ki-Back Chu, Keon-Woong Yoon, Gi-Deok Eom, Jie Mao, Fu-Shi Quan
Summary: This study demonstrates that influenza B virus-like particle vaccines expressing hemagglutinin, neuraminidase, or both antigens can provide cross-protections against mismatched Victoria lineage virus infections, by inducing antibody and T cell responses to reduce lung viral loads and minimize infection outcomes.
Article
Biochemistry & Molecular Biology
Delphine Vincent, AnhDuyen Bui, Doris Ram, Vilnis Ezernieks, Frank Bedon, Joe Panozzo, Pankaj Maharjan, Simone Rochfort, Hans Daetwyler, Matthew Hayden
Summary: This study optimized a LC-MS shotgun quantitative proteomics method to screen wheat genotypes for breeding lines with better performance. A large number of wheat proteins were identified using this method, and data mining tools were used to explore the flour proteome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Xingui Tian, Shelby Landreth, Yao Lu, Kannupriya Pandey, Yan Zhou
Summary: The engineering of the H5 or H7 HA gene in segment 6 affects the immunogenicity of the virus, and PR8-H5-H7(NA) has strong potential to serve as a vaccine candidate against both H5 and H7 subtypes of avian influenza viruses.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Zeinab Karimi, Afsaneh A. Oskouie, Farhad Rezaei, Fatemeh Ajaminejad, Sayed M. Marashi, Talat-Mokhtari Azad
Summary: The study found that the influenza virus infection leads to changes in the metabolic pathways of the organisms, involving biosynthesis pathways such as lipids, polyamines, catecholamines, and vitamins. The metabolism of immune cells can be studied to explain the inflammation caused by the influenza virus.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alexander L. Rusanov, Peter M. Kozhin, Olga Tikhonova, Victor G. Zgoda, Dmitry S. Loginov, Adela Chlastakova, Martin Selinger, Jan Sterba, Libor Grubhoffer, Nataliya G. Luzgina
Summary: The study compared the proteomic profiles of immortalized cell lines and primary cells, finding significant differences in the abundances of various proteins associated with phagocytosis. This suggests that validation of results using PMJ2-R cells as a model for peritoneal macrophages should be carefully conducted.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Yangyang Bian, Florian P. Bayer, Yun-Chien Chang, Chen Meng, Stefanie Hoefer, Nan Deng, Runsheng Zheng, Oleksandr Boychenko, Bernhard Kuster
Summary: Microflow liquid chromatography tandem mass spectrometry (mu LC-MS/MS) has been shown to be a robust and high-throughput method for analyzing proteomes, capable of measuring thousands of proteins from various samples. The system demonstrated the potential for in-depth and high-throughput clinical applications through analyzing a large number of samples over a period of two years.
ANALYTICAL CHEMISTRY
(2021)
Article
Virology
Hae-Ji Kang, Ki-Back Chu, Keon-Woong Yoon, Gi-Deok Eom, Jie Mao, Min-Ju Kim, Su-Hwa Lee, Eun-Kyung Moon, Fu-Shi Quan
Summary: Research suggests that avian influenza VLP vaccines expressing multiple neuraminidases can provide both homologous and heterosubtypic protection against different subtypes, offering a promising approach for developing a universal influenza A vaccine against avian and human influenza virus infections.
Article
Immunology
Diana Aguado-Garcia, Alex Olvera, Christian Brander, Victor Sanchez-Merino, Eloisa Yuste
Summary: The long-term storage stability of vaccines is crucial for the success of global immunization programs. This study evaluated the stability of a HIV-1 virus-like particle vaccine prototype and found that it maintained its structural integrity and the recognition of relevant B-cell epitopes for up to three months at 4 and -20 degrees C. Interestingly, even after a week of storage at room temperature, most particles remained intact and preserved their ability to recognize relevant epitopes.
Article
Chemistry, Multidisciplinary
Elizabeth Q. Littauer, Lisa K. Mills, Nicole Brock, E. Stein Esser, Andrey Romanyuk, Joanna A. Pulit-Penaloza, Elena V. Vassilieva, Jacob T. Beaver, Olivia Antao, Florian Krammer, Richard W. Compans, Mark R. Prausnitz, Ioanna Skountzou
JOURNAL OF CONTROLLED RELEASE
(2018)
Article
Immunology
Ying Liu, Ling Ye, Fang Lin, Yasmine Gomaa, David Flyer, Ricardo Carrion, Jean L. Patterson, Mark R. Prausnitz, Gale Smith, Gregory Glenn, Hua Wu, Richard W. Compans, Chinglai Yang
JOURNAL OF INFECTIOUS DISEASES
(2018)
Article
Multidisciplinary Sciences
Lei Deng, Teena Mohan, Timothy Z. Chang, Gilbert X. Gonzalez, Ye Wang, Young-Man Kwon, Sang-Moo Kang, Richard W. Compans, Julie A. Champion, Bao-Zhong Wang
NATURE COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Lei Deng, Timothy Z. Chang, Ye Wang, Song Li, Shelly Wang, Shingo Matsuyama, Guoying Yu, Richard W. Compans, Jian-Dong Li, Mark R. Prausnitz, Julie A. Champion, Bao-Zhong Wang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Multidisciplinary Sciences
Ying Liu, Ling Ye, Fang Lin, Yasmine Gomaa, David Flyer, Ricardo Carrion, Jean L. Patterson, Mark R. Prausnitz, Gale Smith, Gregory Glenn, Hua Wu, Richard W. Compans, Chinglai Yang
SCIENTIFIC REPORTS
(2018)
Article
Pharmacology & Pharmacy
Min-Chul Kim, Ki-Hye Kim, Jeong Woo Lee, Yu-Na Lee, Hyo-Jick Choi, Yu-Jin Jung, Yu-Jin Kim, Richard W. Compans, Mark R. Prausnitz, Sang-Moo Kang
Article
Chemistry, Multidisciplinary
Jessica C. Joyce, Hila E. Sella, Heather Jost, Matthew J. Mistilis, E. Stein Esser, Pallab Pradhan, Randall Toy, Marcus L. Collins, Paul A. Rota, Krishnendu Roy, Ioanna Skountzou, Richard W. Compans, M. Steven Oberste, William C. Weldon, James J. Norman, Mark R. Prausnitz
JOURNAL OF CONTROLLED RELEASE
(2019)
Article
Microbiology
Ying Liu, Zhiyuan Wen, Ricardo Carrion, Jerritt Nunneley, Hilary Staples, Anysha Ticer, Jean L. Patterson, Richard W. Compans, Ling Ye, Chinglai Yang
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Immunology
Elena V. Vassilieva, Dahnide W. Taylor, Richard W. Compans
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Jacob T. Beaver, Lisa K. Mills, Dominika Swieboda, Nadia Lelutiu, Edward S. Esser, Olivia Q. Antao, Eugenia Scountzou, Dahnide T. Williams, Nikolaos Papaioannou, Elizabeth Q. Littauer, Andrey Romanyuk, Richard W. Compans, Mark R. Prausnitz, Ioanna Skountzou
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2020)
Editorial Material
Pharmacology & Pharmacy
Joo Kyung Kim, Sang-Moo Kang, Richard W. Compans, Bao-Zhong Wang
EXPERT OPINION ON DRUG DISCOVERY
(2021)
Article
Instruments & Instrumentation
Yoo Chun Kim, Jeong Woo Lee, E. Stein Esser, Haripriya Kalluri, Jessica C. Joyce, Richard W. Compans, Ioanna Skountzou, Mark R. Prausnitz
Summary: The study developed a method to incorporate lyophilized influenza vaccine into microneedle patches, enabling stable processing and storage, as well as strong immune responses in a murine model.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2021)
Article
Immunology
Elena V. Vassilieva, Song Li, Heorhiy Korniychuk, Dahnide M. Taylor, Shelly Wang, Mark R. Prausnitz, Richard W. Compans
Summary: New skin vaccination method combining influenza vaccine with recently identified adjuvants has been shown to boost immune protection in aged mice against influenza. The combination of vaccine with a STING pathway activator and saponin-based adjuvant significantly reduces morbidity and residual inflammation in aged mice post vaccination.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Chunhong Dong, Ye Wang, Gilbert X. Gonzalez, Yao Ma, Yufeng Song, Shelly Wang, Sang-Moo Kang, Richard W. Compans, Bao-Zhong Wang
Summary: This study demonstrates that polyethyleneimine-functionalized graphene oxide nanoparticles show superior immune-enhancing properties for influenza vaccines, inducing significantly enhanced and cross-reactive immune responses after intranasal immunization. The nanoparticles provide broad protection against challenges by homologous and heterologous viruses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Virology
Zach Menne, Vasilis C. Pliasas, Richard W. Compans, Sheniqua Glover, Constantinos S. Kyriakis, Ioanna Skountzou
Summary: The study demonstrates that vaccination with neuraminidase VLPs is protective against influenza challenge, and bivalent vaccination can effectively protect against lethal challenge from different subtypes of influenza viruses.