Article
Toxicology
Yukako Komaki, Saki Ono, Takuto Okuya, Yuko Ibuki
Summary: Glucose restriction affects UV-induced DNA damage repair response. Repair process is delayed under low glucose conditions and DNA replication may be suppressed.
TOXICOLOGY IN VITRO
(2023)
Review
Oncology
Davide Valente, Maria Pia Gentileschi, Antonino Guerrisi, Vicente Bruzzaniti, Aldo Morrone, Silvia Soddu, Alessandra Verdina
Summary: This article discusses the induction of gamma H2AX in individuals exposed to ionizing radiation and its applications in clinical studies. By analyzing the various conditions that induce H2AX phosphorylation and describing commonly used methods for gamma H2AX determination, the assessment of DSBs following IR exposure can be optimized.
Article
Biochemistry & Molecular Biology
Ruyuan Yu, Yameng Hu, Shuxia Zhang, Xincheng Li, Miaoling Tang, Meisongzhu Yang, Xingui Wu, Ziwen Li, Xinyi Liao, Yingru Xu, Man Li, Suwen Chen, Wanying Qian, Li-Yun Gong, Libing Song, Jun Li
Summary: In this study, the researchers found that DNA damage triggers the translation of a microprotein called DDUP, which helps to maintain repair complexes at damaged sites and initiate DNA damage repair mechanisms. Inhibition of DDUP by ATR inhibitor increases the sensitivity of cancer cells to DNA-damaging chemotherapeutics.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Analytical
Zhen Wu, Yi Shen, Xumin Zhang
Summary: The study establishes a novel quantitative method, TAG-TMTpro, which triples the multiplexing capacity of TMTpro by introducing Ala or Gly residues to peptides prior to labeling. The method is validated for identification and quantification performance using E. coli and HeLa cell lysates.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Kelsey Gallant, Arsun Bektas, Mary Kaileh, Ana Lustig, Luigi Ferrucci, Gianluca Pegoraro, Tom Misteli
Summary: DNA damage is a prominent biomarker for various diseases and the aging process. Traditional microscopy or cytometric methods are not ideal for large-scale studies. We have developed a high-throughput single-cell assay that accurately measures DNA damage using two major markers. The assay demonstrates sensitivity and low variability in various human immune cells, providing a novel approach for large-scale evaluation of DNA damage.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Xiaofei Yue, Qiqi Jiang, Xuehan Hu, Chunyuan Cen, Songlin Song, Kun Qian, Yuting Lu, Ming Yang, Qian Li, Ping Han
Summary: Quantitative parameters of DECT, specifically AIF and lambda (Hu), show good performance in distinguishing tumor active area, adjacent normal tissue, and tumor necrotic area in HCC patients treated by TACE.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Chen-Hui Cao, Han Ling, Kai Han, Xiao-Peng Lu, Mu-Yan Cai, Jing-Hua Cao, Jie Zhou, Zhi-Cheng Xiang, Jie-Wei Chen, Si Li, Jin-Long Lin, Jin-Ling Duan, Jie Luo, Yu-Jing Fang, Zhi-Zhong Pan, Li Liang, Feng Wang, Dan Xie, Feng-Wei Wang
Summary: PPIP5K2 plays a critical role in enhancing the survival of CRC cells by promoting DNA repair through S1006 dephosphorylation. This study reveals a novel biological function and mechanism model of PPIP5K2, providing a potential therapeutic target for CRC patients.
Article
Oncology
Xuyong Wei, Renyi Su, Mengfan Yang, Binhua Pan, Jun Lu, Hanchao Lin, Wenzhi Shu, Rui Wang, Xiao Xu
Summary: This study explores proteomic profiling in hepatocellular carcinoma (HCC) samples based on alpha-fetoprotein (AFP) subgroups and identifies potential key targets involved in HCC progression. The results reveal four key prognostic biomarkers in HCC and provide opportunities for translational medicine and new treatment.
TRANSLATIONAL ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mateo Glumac, Mirjana Polovic, Anja Batel, Andrea Gelemanovic, Boris Macek, Ana Velic, Ivana Marinovic-Terzic
Summary: DNA-protein crosslinks (DPCs) are toxic lesions that interfere with DNA processing and transcription. The development of an accurate biochemical assay for DPC isolation is crucial for understanding their repair process. The STAR assay is proposed for direct quantification of DPCs under physiologically relevant conditions. Implementation of the STAR assay revealed the presence of small cross-linked peptides on DNA, created by proteolytic degradation of DPCs by SPRTN. This degradation is required for downstream activation of DNA repair and subsequent removal of the cross-linked peptides.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Medicine, Research & Experimental
Dong-Sik Chae, Mi Eun Kim, Kyung-Yil Kang, Nae Yoon Lee, Woo-Suk Lee, Jun Sik Lee
Summary: This study analyzed the protein expression in OA patients according to the International Cartilage Repair Society (ICRS) scale, identifying differential protein expression patterns in ICRS1- and ICRS3-graded OA patients. A total of 231 unique proteins were identified as expressed across the ICRS1 and ICRS3 OA patient groups, shedding light on potential therapeutic targets for OA.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Review
Biotechnology & Applied Microbiology
Junjie Wang, Zeyu Li, Fenfen Yin, Rui Zhang, Ying Zhang, Zhengxin Wang, Xiumei Sheng
Summary: GLIPR1 plays a dual role in cancer, acting as an oncoprotein in some types such as gliomas and breast cancers, while functioning as a tumor suppressor in others like prostate cancers and lung cancers. The mechanisms of GLIPR1 in different tumors remain unclear, but it shows potential in cancer treatment through gene therapy and protein therapy.
CANCER GENE THERAPY
(2022)
Article
Plant Sciences
Wei Du, Chengjiang Ruan, Jingbin Li, He Li, Jian Ding, Siyang Zhao, Xin Jiang
Summary: Yellowhorn seedlings showed changes in protein abundance under drought and heat stress, with a decrease in proteins related to photosynthesis and oxidative phosphorylation, and an increase in heat-shock proteins (HSPs). Fe-Mn SOD enzymes played a crucial role in removing reactive oxygen species (ROS). The results provide insights into the molecular mechanisms of drought and heat stress resistance in yellowhorn and posttranslational modification-related omics of key proteins for future research.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemical Research Methods
Jose Navarrete-Perea, Steven P. Gygi, Joao A. Paulo
Summary: This study analyzed the proteomes of cell lines subjected to acute or chronic nicotine exposures, revealing impacts on mitochondrial protein abundance and immune pathways. The association between nicotine and SARS-CoV2 was also explored, showing time-dependent effects on host proteins interacting with viral proteins. The dataset provides insights into the potential role of nicotine in different biological contexts and implications for studying SARS-CoV2.
JOURNAL OF PROTEOMICS
(2021)
Article
Chemistry, Applied
Zi-Xuan Wu, Yan-Hong Bai, Zi-Ye Wang, Ying-Chen Fan, Liang Song, Yu-Xin Liu, De-Yang Li, Peng-Fei Jiang, Da -Yong Zhou
Summary: The mechanism behind textural changes in scallop adductor muscle during boiling was studied by analyzing the proteins, determining water holding capacity and oxidative indices, and observing the muscle using microscopy techniques. Short-time boiling caused oxidation, denaturation and aggregation of proteins, resulting in contraction and cross-linking of muscle fibers, leading to an increase in hardness and shear force. Long-time boiling caused degradation of structural proteins, leading to a loose muscle fiber network and a decrease in hardness and shear force.
Article
Plant Sciences
Alpa Puri, Aga Pajak, Abdelali Hannoufa, Frederic Marsolais
Summary: This study investigates the effects of miR156 overexpression on protein level changes under drought stress in alfalfa. The results suggest that miR156 overexpression alters protein expression and is associated with enhanced drought stress tolerance.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)