Article
Geriatrics & Gerontology
Hongchun Lin, Xinxin Ma, Yuxiang Sun, Hui Peng, Yanlin Wang, Sandhya Sara Thomas, Zhaoyong Hu
Summary: This study utilized snRNA-seq to investigate transcriptional changes associated with muscle atrophy induced by denervation. The results revealed a significant increase in the proportion of type IIb2 myonuclei and robust transcriptional reprogramming in response to denervation. Gene regulatory network analysis showed enhanced activity of specific genes in denervated myonuclei, along with alterations in metabolic pathways and cell-cell communication.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Nutrition & Dietetics
Lizhen Zheng, Le Huang, Ziyi Chen, Can Cui, Ri Zhang, Ling Qin
Summary: The study demonstrated that Mg++ supplementation successfully alleviated CS-induced muscle atrophy in rats at both functional and morphology levels, indicating translational potential for patients. This study provided evidence for the first time that Mg++ supplementation could prevent muscle atrophy, an adverse effect of CS therapy, also adopted for treating COVID-19.
EUROPEAN JOURNAL OF NUTRITION
(2021)
Article
Agriculture, Dairy & Animal Science
Wenqian Jia, Lei Wu, Zhong Zhuang, Minghong Xu, Yijia Lu, Zhixiu Wang, Hao Bai, Guohong Chen, Guobin Chang, Yong Jiang
Summary: This study investigated the molecular mechanism of threonine regulation on breast muscle development in Pekin ducks. The results showed that threonine intake level affected body weight and breast fiber size in Pekin ducks, and regulated the expression of many genes. This study provides new insights into the molecular mechanisms underlying breast muscle development in ducks.
Article
Endocrinology & Metabolism
Rui M. Patricio Da Silva-Junior, Ana Carolina Bueno, Clarissa Silva Martins, Fernanda Coelli-Lacchini, Jorge Guilherme Okanobo Ozaki, Danillo Cunha De Almeida-E-Silva, Junier Marrero-Gutierrez, Antonio Carlos dos Santos, Carlos Garcia-Peral, Helio Rubens Machado, Marcelo Volpon dos Santos, Paula Lamparelli Elias, Ayrton C. Moreira, Leandro M. Colli, Ricardo Z. N. Vencio, Sonir R. Antonini, Margaret de Castro
Summary: This study explored pituitary tumors in a heterogeneous ethnic population by evaluating methylome and transcriptome signatures. Unsupervised hierarchical clustering analysis revealed three clusters associated with functioning, nonfunctioning, and ACTH-secreting tumors. Differential methylated and expressed genes were found to be associated with tumor clinical presentation and WHO classifications. The clustering of pituitary development/differentiation-related transcripts also supported the classification. Making the data publicly available enables further research on the pathogenesis and prognosis of pituitary tumors.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Biotechnology & Applied Microbiology
Jie Liu, Fuwei Li, Xin Hu, Dingguo Cao, Wei Liu, Haixia Han, Yan Zhou, Qiuxia Lei
Summary: The study identified 337 differentially expressed miRNAs during chicken muscle development, with 106 downregulated and 44 upregulated miRNAs. Upregulated miRNAs mainly contributed to cellular turnover, while downregulated miRNAs played a regulatory role in protein turnover metabolism.
Article
Biology
Xuebin Zhang, Penghu Lian, Mingming Su, Zhigang Ji, Jianhua Deng, Guoyang Zheng, Wenda Wang, Xinyu Ren, Taijiao Jiang, Peng Zhang, Hanzhong Li
Summary: In this study, single-cell RNA sequencing was used to analyze a rare case of ectopic ACTH&CRH-secreting pheochromocytoma, leading to the identification of a novel multi-functionally chromaffin-like cell type. The molecular mechanism behind Cushing's syndrome in this rare case was hypothesized to involve this newly identified tumor cell type. Immunohistochemistry results confirmed the unique properties of this cell type, along with the heterogeneous endothelial and immune microenvironment in different adrenal tumor subtypes.
Article
Gastroenterology & Hepatology
Shvetank Sharma, Sukriti Baweja, Jaswinder S. Maras, Saggere M. Shasthry, Richard Moreau, Shiv K. Sarin
Summary: In patients with severe alcoholic hepatitis, those who do not respond to steroids show higher activity of gene modules related to T and B cells, NK cells, and mitochondrial electron transport chain in PBMCs at baseline and during corticosteroid therapy compared to steroid responders.
Article
Genetics & Heredity
Svetlana Yegorova, Oleg Yegorov, Leonardo F. Ferreira
Summary: The study identified 112 differentially expressed genes in the diaphragm post-chronic myocardial infarction, suggesting pathological remodeling and potential biological targets related to neuromuscular junction, extracellular matrix, metabolism and iron homeostasis.
Article
Agriculture, Dairy & Animal Science
Siyuan Zhan, Hongfan Zhai, Min Tang, Yanan Xue, Dandan Li, Linjie Wang, Tao Zhong, Dinghui Dai, Jiaxue Cao, Jiazhong Guo, Li Li, Hongping Zhang
Summary: This study used RNA sequencing to analyze the gene expression during the differentiation of skeletal muscle satellite cells in goats and identified 2551 differentially expressed genes. Functional enrichment analysis revealed their involvement in muscle development-related biological processes and signaling pathways, providing novel insights into muscle growth and development in goats.
Article
Biotechnology & Applied Microbiology
Lingtong Ren, Anfang Liu, Qigui Wang, Honggan Wang, Deqiang Dong, Lingbin Liu
Summary: This study explored the embryonic muscle development of Chengkou Mountain Chicken, identifying numerous differentially expressed genes related to muscle development. Key genes for embryonic development were validated, providing a preliminary explanation of their role in muscle development. Overall, this study broadened our understanding of the phenotypic mechanism for myofiber formation and offered valuable information for enhancing chicken quality.
Article
Cell & Tissue Engineering
Yuan-chi Teng, Alfredo Leonardo Porfirio-Sousa, Giulia Magri Ribeiro, Marcela Corso Arend, Lindolfo da Silva Meirelles, Elizabeth Suchi Chen, Daniela Santoro Rosa, Sang Won Han
Summary: This study investigated the gene expression profiles of pericytes during early stages of limb ischemia, showing that ischemia triggered proliferation, migration, and upregulation of myogenesis-related transcripts. The transcriptomic analysis also revealed that pericytes induced or upregulated the expression of cytokines with effects on endothelial cells, leukocyte chemoattraction, or the activation of inflammatory cells. These findings provide insights into skeletal muscle pericyte biology under ischemic conditions and may be beneficial for developing novel pericyte-based therapies.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Genetics & Heredity
Yueying Yun, Rihan Wu, Xige He, Xia Qin, Lu Chen, Lina Sha, Xueyan Yun, Tadayuki Nishiumi, Gerelt Borjigin
Summary: This study investigated the expression profiles of miRNAs and mRNAs in the skeletal muscle of Wuranke sheep, and identified potential miRNA-mRNA pairs that may act as regulators during muscle development.
Article
Agriculture, Dairy & Animal Science
Zhaozheng Yin, Wei Zhou, Haiguang Mao, Xinyang Dong, Xuan Huang, Haiyang Zhang, Honghua Liu
Summary: This study identified differentially expressed genes related to muscle growth and lipid metabolism in domestic pigeons by analyzing the transcriptomes of breast muscle and liver. The identified genes are involved in cell proliferation and differentiation, lipid metabolism, and energy metabolism. The findings lay the foundation for understanding the mechanisms of muscle growth and lipid metabolism in domestic pigeons.
Article
Agriculture, Dairy & Animal Science
Narayana H. H. Mohan, Prajwalita Pathak, Lipika Buragohain, Juri Deka, Jaya Bharati, Anil Kumar Das, Rajendran Thomas, Rajendra Singh, Dilip Kumar Sarma, Vivek Kumar Gupta, Bikash Chandra Das
Summary: Muscle development is vital in pig breeding programs, but the mechanisms regulating myogenesis remain unclear. Integrating transcriptome data with histology and meat yield, this study compared two pig breeds (Mali and Hampshire) to identify differences in myogenesis during early growth. The analysis revealed differentially expressed genes and highlighted the importance of myoblast fusion and myogenic activities in breed muscularity. The results could aid in selecting animals with desired muscularities.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Veterinary Sciences
Lihong Gu, Qicheng Jiang, Youyi Chen, Xinli Zheng, Hailong Zhou, Tieshan Xu
Summary: This study found that miRNAs, in conjunction with m6A modification, played a key role in the embryonic breast muscle development of Wenchang chickens. The results of this paper offer a theoretical basis for the study of m6A function in muscle development and fat deposition of Wenchang chickens.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Saw Simeon, Watshara Shoombuatong, Nuttapat Anuwongcharoen, Likit Preeyanon, Virapong Prachayasittikul, Jarl E. S. Wikberg, Chanin Nantasenamat
JOURNAL OF CHEMINFORMATICS
(2016)
Article
Infectious Diseases
Warawut Laolerd, Yukihiro Akeda, Likit Preeyanon, Panan Ratthawongjirakul, Pitak Santanirand
MICROBIAL DRUG RESISTANCE
(2018)
Article
Chemistry, Multidisciplinary
Naravut Suvannang, Likit Preeyanon, Aijaz Ahmad Malik, Nalini Schaduangrat, Watshara Shoombuatong, Apilak Worachartcheewan, Tanawut Tantimongcolwat, Chanin Nantasenamat
Article
Biodiversity Conservation
Molly C. McCormley, Cory D. Champagne, Jared S. Deyarmin, Alicia P. Stephan, Daniel E. Crocker, Dorian S. Houser, Jane Khudyakov
CONSERVATION PHYSIOLOGY
(2018)
Article
Multidisciplinary Sciences
J. I. Khudyakov, C. D. Champagne, L. M. Meneghetti, D. E. Crocker
SCIENTIFIC REPORTS
(2017)
Article
Biology
Jane Khudyakov, Jared S. Deyarmin, Ryan M. Hekman, Laura Pujade Busqueta, Rasool Maan, Melony J. Mody, Reeti Banerjee, Daniel E. Crocker, Cory D. Champagne
Article
Multidisciplinary Sciences
Jared S. Deyarmin, Molly C. McCormley, Cory D. Champagne, Alicia P. Stephan, Laura Pujade Busqueta, Daniel E. Crocker, Dorian S. Houser, Jane Khudyakov
SCIENTIFIC REPORTS
(2019)
Article
Physiology
Jane I. Khudyakov, Eileen Abdollahi, Angela Ngo, Gureet Sandhu, Alicia Stephan, Daniel P. Costa, Daniel E. Crocker
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Jared Deyarmin, Ryan Hekman, Cory Champagne, Molly McCormley, Alicia Stephan, Daniel Crocker, Dorian Houser, Jane Khudyakov
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2020)
Article
Physiology
Jane Khudyakov, Michael D. Treat, Mikayla C. Shanafelt, Jared S. Deyarmin, Benjamin A. Neely, Frank van Breukelen
Summary: The study on tenrecs reveals that there is significant variability in liver protein abundance during torpor, indicating poor control of proteostasis. Compared to active warm animals, protein abundance in active cold and torpid tenrecs is more unstable.
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2021)
Article
Physiology
Julia Maria Torres-Velarde, Sree Rohit Raj Kolora, Jane Khudyakov, Daniel E. Crocker, Peter H. Sudmant, Jose Pablo Vazquez-Medina
Summary: The study investigates how elephant seal muscle cells adapt to prolonged glucocorticoid exposure using a cellular model and transcriptomic, metabolic, and morphological analyses. The findings suggest that prolonged exposure to dexamethasone induces changes in gene expression related to muscle structure, energy metabolism, and cell survival pathways. Additionally, it leads to a metabolic shift towards glycolysis, alterations in mitochondrial morphology, and decreased mitochondria-endoplasmic reticulum interactions, all without compromising cell viability.
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2021)
Article
Endocrinology & Metabolism
Caroline L. Rzucidlo, Emily S. Sperou, Rachel R. Holser, Jane Khudyakov, Daniel P. Costa, Daniel E. Crocker
Summary: Adipose tissue is crucial for thermal regulation and energy storage in endotherms, and also acts as an endocrine organ. Adipokines play important roles in regulating metabolism, energy expenditure, reproduction, and immune function, with significant variations observed in circulating levels among female northern elephant seals at different life-history stages. These adipokines are closely associated with changes in body condition and may regulate important physiological processes in northern elephant seals.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2021)
Article
Biology
Jane Khudyakov, Rachel R. Holser, Craig A. Vierra, Serena T. Ly, Theron K. Niel, Basma M. Hasan, Daniel E. Crocker, Daniel P. Costa
Summary: Northern elephant seals undergo prolonged fasting during energy-intensive life-history stages and display unique metabolic features. Changes in apolipoprotein composition may play a role in maintaining high HDL levels in these seals.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2022)
Article
Physiology
Elizabeth R. Piotrowski, Michael S. Tift, Daniel E. Crocker, Anna B. Pearson, Jose P. Vazquez-Medina, Anna D. Keith, Jane I. Khudyakov
Summary: Marine mammals like northern elephant seals have adaptations to deep dives, including increased antioxidants and high hemoprotein content for oxygen storage. Carbon monoxide produced by heme turnover has cytoprotective effects in these animals, and muscle CO levels and gene expression associated with CO production both increase with age. Ontogenetic changes in gene expression suggest mechanisms for transitioning to a deep-diving lifestyle.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biodiversity Conservation
Laura Pujade Busqueta, Daniel E. Crocker, Cory D. Champagne, Molly C. McCormley, Jared S. Deyarmin, Dorian S. Houser, Jane Khudyakov
CONSERVATION PHYSIOLOGY
(2020)