Editorial Material
Clinical Neurology
Noor Lail, Ashutosh K. Pandey, Sundararajan Venkatesh, Roberto D. Noland, Gabriel Swanson, Debkumar Pain, Helen M. Branson, Carolyn K. Suzuki, Grace Yoon
Summary: This article describes the diagnostic odyssey of a 4-year-old female patient with ACO2-related infantile cerebellar retinal degeneration, a rare condition. Whole-exome sequencing identified two variants in the ACO2 gene, one likely pathogenic and the other uncertain significance. Functional biochemical studies confirmed the diagnosis.
Article
Endocrinology & Metabolism
Giuseppe Grande, Ferran Barrachina, Ada Soler-Ventura, Meritxell Jodar, Francesca Mancini, Riccardo Marana, Sabrina Chiloiro, Alfredo Pontecorvi, Rafael Oliva, Domenico Milardi
Summary: This study used proteomic profiling to analyze alterations of sperm protein composition in secondary male hypogonadism. Several differentially expressed proteins were identified and may serve as new targets for infertility treatments due to androgen deficiency.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Plant Sciences
Jia Hao, Qiang Li, Hongjun Yu, Heng Wang, Lin Chai, Tiantian Miao, Weijie Jiang
Summary: Nitrogen deficiency has a significant impact on the metabolic pathways of cucumber fruit, especially in carbon metabolism, amino acid biosynthesis, and ascorbate metabolism. The research results indicate that under nitrogen deficiency, protein expression in cucumber fruit is altered.
HORTICULTURAL PLANT JOURNAL
(2021)
Article
Cell Biology
Hong-Ying Li, Ting-Ting Wei, Miao Zhuang, Cheng-Ye Tan, Tian-Hua Xie, Jiping Cai, Yong Yao, Lingpeng Zhu
Summary: Cellular senescence is a hallmark of aging. This study reveals that iron derived from NCOA4-mediated ferritinophagy causes cellular senescence via the cGAS-STING pathway. Inhibiting iron accumulation may represent a promising therapeutic approach for age-related diseases such as AMD.
CELL DEATH DISCOVERY
(2023)
Review
Biotechnology & Applied Microbiology
Huikyong Cho, Nadia Bouain, Luqing Zheng, Hatem Rouached
Summary: Phosphorus is essential for all living organisms, especially for plants which require a large amount to grow. The challenge of addressing phosphorus deficiency and scarcity, due to the finite natural resource of phosphate rock, is exacerbated by the increasing demand for food as the global population grows. Advancements in understanding plant mechanisms to regulate phosphorus homeostasis offer opportunities for further research and innovative strategies to improve phosphorus nutrition in plants, addressing a pressing challenge in agriculture in the twenty first century.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Laura Ceballos-Laita, Daisuke Takahashi, Matsuo Uemura, Javier Abadia, Ana Flor Lopez-Millan, Jorge Rodriguez-Celma
Summary: Iron and manganese are essential elements for plants, but they compete for the same uptake transporters and show conflicting interactions at the regulatory level. Proteomic techniques were used to study the proteome profiles of tomato roots grown under iron or manganese deficiency. The results showed that both deficiencies led to common cell wall remodelling, but differed greatly in terms of oxidative stress, coumarin production, protein, nitrogen, and energy metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Khadidja Guehlouz, Thomas Foulonneau, Patrizia Amati-Bonneau, Majida Charif, Estelle Colin, Celine Bris, Valerie Desquiret-Dumas, Dan Milea, Philippe Gohier, Vincent Procaccio, Dominique Bonneau, Johan T. den Dunnen, Guy Lenaers, Pascal Reynier, Marc Ferre
Summary: The study established an LSDB for ACO2 using HPO vocabulary, incorporating all variants and clinical cases. The results suggest that the clinical spectrum of ACO2 patients should be considered as a continuum of symptoms and refine the classification of some common variants.
Article
Plant Sciences
Lu Zheng, Mohammad Rezaul Karim, Yin-Gang Hu, Renfang Shen, Ping Lan
Summary: The study revealed that the low-Pi-tolerant wheat cultivar KN199 exhibited greater root adaptations and primary metabolic changes compared to CS under Pi deficiency, shedding light on the molecular mechanisms underlying Pi-deficiency tolerance in wheat and strategies for improving Pi efficiency.
Article
Biochemistry & Molecular Biology
Ling Cheng, Wanling Min, Man Li, Lili Zhou, Chuan-Chih Hsu, Xuelian Yang, Xue Jiang, Zhijie Ruan, Yongjia Zhong, Zhi-Yong Wang, Wenfei Wang
Summary: Under phosphate starvation conditions, the quantity of differential proteins in soybean leaves significantly increases, with some involved in translation processes and others accumulated in chlorophyll biosynthetic and carbon metabolic processes. Among the induced proteins, enolase protein GmENO2a is the most significant. Additionally, GmENO2a and GmENO2b are dramatically induced at the transcriptional level, and only these two genes contain the P1BS motif.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Huimin Yan, Yale Wang, Bo Chen, Weijie Wang, Hongzheng Sun, Huwei Sun, Junzhou Li, Quanzhi Zhao
Summary: This study investigated the function of OsCKX2 gene in response to phosphate deficiency in rice. The results showed that knockout of OsCKX2 enhanced tolerance to phosphate deficiency, while overexpression reduced tolerance. Transcriptome and metabolome analyses revealed that overexpression of OsCKX2 downregulated the expression of genes related to phosphate transport, membrane lipid metabolism, and glycolysis, leading to decreased phosphate uptake and utilization. Conversely, knockout of OsCKX2 upregulated the expression of phosphate transport genes and increased the consumption of metabolites in membrane lipid metabolism and glycolysis.
Article
Engineering, Environmental
Mi Feng, Jiahua Zhou, Xiaolong Yu, Hao Wang, Yushuo Guo, Wei Mao
Summary: This study demonstrated the potential of white rot fungi Pycnoporus sanguineus in biodegrading triphenyl phosphate (TPhP) and identified the degradation pathway involving oxidative cleavage, hydroxylation, and methylation. The biotreatment by P. sanguineus significantly reduced the biotoxicity of TPhP, and its cooperation with indigenous degraders promoted efficient TPhP biodegradation in the water-sediment system. This research provided insights into the mechanisms, pathways, and risk assessment of TPhP biodegradation by P. sanguineus, confirming the feasibility of using this fungus for TPhP bioremediation applications.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Review
Plant Sciences
Suchismita Prusty, Ranjan Kumar Sahoo, Subhendu Nayak, Sowmya Poosapati, Durga Madhab Swain
Summary: Micronutrients are essential for plant growth, productivity and reproduction. Currently, eight trace elements including Fe, Zn, Mn, Cu, Ni, B, Mo, and Cl are considered essential for higher plants. With advancements in nutrient solution culture techniques and analytical instrumentation, more essential elements could be discovered. The physiology of micronutrient absorption, translocation and deposition in plants, as well as their functions in plant growth and development, still require further investigation. Proteomic and genomic approaches have been used to explore these questions.
Article
Plant Sciences
Nicolas Marro, Javier Lidoy, Maria Angeles Chico, Carlos Rial, Juan Garcia, Rosa M. Varela, Francisco A. Macias, Maria J. Pozo, Martina Janouskova, Juan A. Lopez-Raez
Summary: Nitrogen (N) and phosphorus (P) are essential macronutrients for plant growth, and Strigolactones (SLs) play a key role in regulating plant responses to phosphorus deficiency and mediating the N-P signaling interplay. SLs prioritize nitrogen status over phosphorus status in plants, affecting biosynthesis and modulating the expression of key regulatory genes in phosphate and nitrate signaling pathways. This suggests that SLs are involved in more physiological processes than previously proposed.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Maddie E. James, Henry Arenas-Castro, Jeffrey S. Groh, Scott L. Allen, Jan Engelstadter, Daniel Ortiz-Barrientos
Summary: This study reveals multiple origins within ecotypes of an Australian wildflower, Senecio lautos, implying that selection can repeatedly create similar phenotypes despite the low level of gene flow between different environments.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Pharmacology & Pharmacy
Heng-li Tong, Hao Chen, Fei-peng Gong, Ling-yun Zhong, Jing Zhu, Song-hong Yang
Summary: The study found that both ULRA and YF had therapeutic effects on rats with Yang deficiency syndrome, improving gonadal morphology and increasing serum cyclic adenosine monophosphate and cyclic guanosine monophosphate levels. After processing, ULRA into YF led to changes in alkaloid composition, with a decrease in C19-diester-diterpenoid alkaloids and an increase in C20-diterpene alkaloids. Proteomics analysis showed that ULRA mainly affected muscle contraction-related proteins, while YF mainly influenced mitochondrial proteins and adenosine triphosphate energy metabolism.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Paul Lesueur, Francois Chevalier, Elias A. El-Habr, Marie-Pierre Junier, Herve Chneiweiss, Laurent Castera, Etienne Mueller, Dinu Stefan, Yannick Saintigny
SCIENTIFIC REPORTS
(2018)
Article
Cell Biology
Charlotte Lepleux, Aurelie Marie-Brasset, Mihaela Temelie, Marion Boulanger, Emilie Brotin, Mary B. Goldring, Christophe Hirtz, Guillaume Vares, Tetsuo Nakajima, Yannick Saintigny, Diana Savu, Francois Chevalier
JOURNAL OF CELL COMMUNICATION AND SIGNALING
(2019)
Article
Oncology
Mathieu Cesaire, Utpal Ghosh, Jean-Baptiste Austry, Etienne Muller, Francesco Paolo Cammarata, Marilyne Guillamin, Massimo Caruso, Laurent Castera, Giada Petringa, Giuseppe Antonio Pablo Cirrone, Francois Chevalier
JOURNAL OF BONE ONCOLOGY
(2019)
Review
Biochemistry & Molecular Biology
Juliette Thariat, Samuel Valable, Carine Laurent, Siamak Haghdoost, Elodie A. Peres, Myriam Bernaudin, Francois Sichel, Paul Lesueur, Mathieu Cesaire, Edwige Petit, Aurelie E. Ferre, Yannick Saintigny, Sven Skog, Mihaela Tudor, Michael Gerard, Sebastien Thureau, Jean-Louis Habrand, Jacques Balosso, Francois Chevalier
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Editorial Material
Biochemistry & Molecular Biology
Francois Chevalier
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Oncology
Guillaume Vares, Vidhula Ahire, Shigeaki Sunada, Eun Ho Kim, Sei Sai, Francois Chevalier, Paul-Henri Romeo, Tadashi Yamamoto, Tetsuo Nakajima, Yannick Saintigny
RADIOTHERAPY AND ONCOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Mihaela Tudor, Antoine Gilbert, Charlotte Lepleux, Mihaela Temelie, Sonia Hem, Jean Armengaud, Emilie Brotin, Siamak Haghdoost, Diana Savu, Francois Chevalier
Summary: The study revealed that irradiated cells can induce stress signal transmission in surrounding non-irradiated cells, triggering radiation-induced bystander effects. Proteomic analysis showed that the conditioned medium of irradiated chondrosarcoma cells caused significant cellular responses in chondrocytes, including oxidative stress, cellular motility, and exosomes pathways. Secretomic analysis also identified specific proteins in the conditioned medium of irradiated cells, such as stress granule-related proteins and proteins involved in the cellular response to DNA damage stimuli.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Mathieu Cesaire, Juliette Montanari, Hubert Curcio, Delphine Lerouge, Radj Gervais, Pierre Demontrond, Jacques Balosso, Francois Chevalier
Summary: The poor survival of unresectable locally advanced stage non-small cell lung cancer is mainly due to resistance to chemoradiotherapy. Despite recent advancements in adjuvant immunotherapy, the majority of patients still face low survival rates.
Article
Biochemistry & Molecular Biology
Maria Grazia Andreassi, Nadia Haddy, Mats Harms-Ringdahl, Jonica Campolo, Andrea Borghini, Francois Chevalier, Jochen M. Schwenk, Brice Fresneau, Stephanie Bolle, Manuel Fuentes, Siamak Haghdoost
Summary: The Health Effects of Cardiac Fluoroscopy and Modern Radiotherapy (photon and proton) in Pediatrics (HARMONIC) is a five-year project funded by the European Commission with the aim of improving understanding of long-term ionizing radiation risks for pediatric patients. This paper provides a detailed overview of the project's rationale, design, and methods for the biological aspect, with objectives to understand the molecular pathways and identify potential biomarkers involved in long-term health risks. Biological samples will be collected at three time points, and state-of-the-art analytical methods will be used to assess biomarker levels and explore proteomics and miRNA transcriptomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Antoine Gilbert, Mihaela Tudor, Juliette Montanari, Kevin Commenchail, Diana Iulia Savu, Paul Lesueur, Francois Chevalier
Summary: This review article describes the origins of chondrosarcoma radiation-resistance and proposes potential solutions to improve treatment based on tumor grade and characteristics. Chondrosarcoma, a chemoresistant and radioresistant tumor, is difficult to treat with surgery due to its location. The resistance is attributed to the composition of the tumor, which creates a low oxygen tension microenvironment causing the cells to dedifferentiate into cancer stem cells. Hadrontherapy, with its ballistic properties and greater biological effectiveness, combined with targeted inhibitors, is considered a promising option for treating high-grade chondrosarcoma.
Review
Biochemistry & Molecular Biology
Francois Chevalier, Thomas Schlatholter, Jean-Christophe Poully
Summary: This review mainly investigates the radiation-induced intra- and inter-molecular transfer of charge, atoms, and energy within biomolecular systems in the gas phase. It discusses the building blocks of DNA, proteins, saccharides, and antibiotics, highlighting the emergence of general processes as well as their timescales and mechanisms.
Article
Multidisciplinary Sciences
Mihaela Tudor, Roxana Cristina Popescu, Raluca D. Negoita, Antoine Gilbert, Mihaela A. Ilisanu, Mihaela Temelie, Anca Dinischiotu, Francois Chevalier, Mona Mihailescu, Diana Iulia Savu
Summary: This study used polyethylene glycol-encapsulated iron oxide nanoparticles for intracellular delivery of the chemotherapeutic doxorubicin to enhance the cytotoxic effects of carbon ions. The combination of IONPDOX with ion carbon or photon irradiation increased the lethal effects of radiation and induced micronuclei production. Changes in hyperspectral images and spectral profiles of nuclei reflected biological modifications in CHS cells after treatment and may serve as new spectroscopic markers for cancer therapy effects.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Antoine Gilbert, Valentin Payet, Benoit Bernay, Elisabeth Chartier-Garcia, Isabelle Testard, Serge M. Candeias, Francois Chevalier
Summary: This study investigated the bystander effects in non-irradiated chondrocytes that received conditioned medium from irradiated chondrosarcoma cells. The results showed that the protein translation pathway, inflammatory cytokine secretion, and oxidative stress pathway were affected in the bystander chondrocytes. Additionally, changes in the regulation of chromosome separation were observed in response to low-dose X-ray and carbon-ion irradiation. These findings provide important insights into the mechanisms of bystander effects.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2022)
Review
Oncology
Juliette Thariat, Francois Chevalier, Daniel Orbach, Luc Ollivier, Pierre-Yves Marcy, Nadege Corradini, Arnaud Beddok, Nicolas Foray, Gaelle Bougeard
Summary: Managing cancer patients with Li-Fraumeni or heritable TP53-related cancer syndromes is challenging due to their increased risk of second malignant neoplasms after genotoxic stresses like systemic treatments or radiotherapy. Decision making must balance risks of normal tissue toxicity, tumour response, and overall prognosis. Avoiding radiotherapy is recommended, but in cases where it is necessary, adaptations to minimize risks should be considered. Research on approaches reducing misrepaired DNA damage is ongoing.
Article
Oncology
Francois Chevalier, Dounia Houria Hamdi, Charlotte Lepleux, Mihaela Temelie, Anais Nicol, Jean Baptiste Austry, Paul Lesueur, Guillaume Vares, Diana Savu, Tetsuo Nakajima, Yannick Saintigny
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
(2019)