Article
Biotechnology & Applied Microbiology
Hyun-Sik Yun, Young-Saeng Kim, Ho-Sung Yoon
Summary: The study found that the addition of glucose under mixotrophic conditions improved biomass yield and the content of useful substances in microalgae. Optimal cultivation results were obtained when 15-20 g L-1 initial glucose was provided under mixotrophic conditions.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
Chengdeng Chi, Youcai Zhou, Feiyang Cheng, Xu Guo, Bilian Chen, Yiping Zhang, Yongjin He
Summary: Chlorella sp. MBFJNU-17, a strain rich in starch and protein, can be obtained by adjusting the glucose and urea content during cultivation. The structure and digestibility of the starch produced by MBFJNU-17 cells can be modulated by regulating the glucose and urea content in heterotrophic cultivation conditions.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Green & Sustainable Science & Technology
Yun Huang, Peirong Li, Yong Huang, Ao Xia, Xun Zhu, Qiang Liao
Summary: Microalgae biofilm is a porous structure that achieves simultaneous growth and lipid enrichment through photoautotrophic-heterotrophic symbiosis, solving the contradiction between microalgae growth and lipid accumulation, and providing a new approach for converting wastewater resources into clean biofuels.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Plant Sciences
Muhammad Sarwar Khan, Rimsha Riaz, Muhammad Majid, Kashif Mehmood, Ghulam Mustafa, Faiz Ahmad Joyia
Summary: The plastid YCF4 gene plays a critical role in regulating plastid gene expression and assembling the photosynthetic complex. Complete knockout of the YCF4 gene in tobacco chloroplasts resulted in the inability of plants to survive photoautotrophically and showed structural anomalies in chloroplasts. In addition, the C-terminus of YCF4 was found to interact with other chloroplast proteins, highlighting its importance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Liangke Chen, Xiangbai Dong, Huifang Yang, Yaru Chai, Yan Xia, Lihong Tian, Le Qing Qu
Summary: Cytosolic DISPROPORTIONATING ENZYME2 (DPE2) converts maltose to glucose, providing energy and cellular materials for pollen germination and pollen tube elongation in rice. Deficiency of DPE2 leads to defective germination and elongation of pollen, while Pho2 might partly compensate for the deficiency of DPE2.
Article
Environmental Sciences
Hongyan Ren, Guoqing Zhu, Jing Ni, Mingwei Shen, Pau Loke Show, Fubao Fuelbiol Sun
Summary: Utilizing starch wastewater for microalgae biomass production is a promising approach due to the nutrients present. However, poor photosynthetic efficiency hinders biomass production. A photo-regulation strategy involving adjusting light/dark cycles and wavelengths has been shown to enhance intracellular photo electron transfer and increase biomass accumulation. This strategy has potential for improving microalgae growth and productivity.
Article
Chemistry, Medicinal
Bhavitavya Nijampatnam, Parmanand Ahirwar, Piyasuda Pukkanasut, Holly Womack, Luke Casals, Hua Zhang, Xia Cai, Suzanne M. Michalek, Hui Wu, Sadanandan E. Velu
Summary: Dental caries is a bacterial infectious disease characterized by demineralization of tooth enamel, mainly caused by Streptococcus mutans forming biofilms. Inhibiting the glucosyl transferases of S. mutans may be an effective approach to develop selective biofilm inhibitors.
ACS MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Agricultural Engineering
Xuehua Xiao, Youcai Zhou, Zhibo Liang, Rongzhao Lin, Mingmin Zheng, Bilian Chen, Yongjin He
Summary: The study established an efficient two-stage cultivation process to produce microalgal biomass rich in protein by optimizing nutrient conditions and gene expression. Chlorella sp. MBFJNU-17 showed promising potential for producing high-value biomass enriched in protein.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Sachin Vyas, Alok Patel, Eric Nabil Risse, Eleni Krikigianni, Ulrika Rova, Paul Christakopoulos, Leonidas Matsakas
Summary: Heterotrophic cultivation of microalgae using spruce hydrolysate instead of glucose offers a suitable biorefinery concept at large-scale for biodiesel-grade lipids production, whereas photoautotrophic bioreactors are recommended for sustainable protein and lutein biosynthesis.
BIORESOURCE TECHNOLOGY
(2022)
Article
Plant Sciences
Aakanksha Singh, Julia Compart, Shadha Abduljaleel AL-Rawi, Harendra Mahto, Abubakar Musa Ahmad, Joerg Fettke
Summary: The non-catalytic starch-binding proteins LESV and ESV1 play a crucial role in starch metabolism, affecting phosphorylation, hydrolysis and synthesis processes. They also induce stable alterations in the glucan structures at the surface of starch granules.
Article
Biochemistry & Molecular Biology
Xiangfeng Niu, Ethan Stancliffe, Susan J. Gelman, Lingjue Wang, Michaela Schwaiger-Haber, Joe L. Rowles, Leah P. Shriver, Gary J. Patti
Summary: In this study, an approach to resolve the fluxes of NADPH in the cytosol and mitochondria was introduced using deuterium tracing. It was found that challenges in the cytosol affected NADPH fluxes in the cytosol, while challenges in the mitochondria affected NADPH fluxes in the mitochondria. This work highlights the value of using proline labeling as a reporter system for studying compartmentalized metabolism and reveals independent regulation of NADPH homeostasis in the cytosol and mitochondria, without evidence for NADPH shuttle activity.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Plant Sciences
Cristal Lopez-Gonzalez, Sheila Juarez-Colunga, Samuel Trachsel, Nayelli Marsch-Martinez, C. Stewart Gillmor, Axel Tiessen
Summary: This study investigates the carbon allocation between different tissues in maize at the early reproductive stages, revealing the key role of sugar transporters in sugar partitioning. The findings highlight the differences between the female inflorescence and the top internode of the stem during grain filling.
Article
Plant Sciences
Francisco M. Gamez-Arjona, Angel Merida
Summary: This study analyzed the impact of the structure of the N-terminal region of starch synthase SS3 on the enzyme's localization pattern, finding that the balance of interactions between different domains in the N-terminal region leads to the formation of the localization pattern.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Imke I. Hoermiller, Dietmar Funck, Lilli Schoenewolf, Henrik May, Arnd G. Heyer
Summary: Proline, an amino acid that accumulates in plants under abiotic stress, plays an important role in freezing tolerance in Arabidopsis thaliana. Mutants with altered proline allocation and localization showed reduced basal freezing tolerance, indicating the significance of cytosolic proline. Additionally, the starch excess 1-1 mutant affected photosynthetic electron transport and increased sensitivity to cold stress.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Xiaorui Fan, William D. Barshop, Ajay A. Vashisht, Vijaya Pandey, Stephanie Leal, Shima Rayatpisheh, Yasaman Jami-Alahmadi, Jihui Sha, James A. Wohlschlegel
Summary: This study investigated the mechanism of the cytosolic iron-sulfur cluster assembly (CIA) pathway using targeted proteomics. The results showed that certain CIA components and substrates interact with the CIA scaffold complex and the CIA targeting complex. Moreover, these interactions are regulated by environmental cues such as iron and oxygen availability. These findings suggest that the CIA pathway adapts to changes in cellular environment through complex reorganization.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Marta Gietler, Malgorzata Nykiel, Slawomir Orzechowski, Joerg Fettke, Barbara Zagdanska
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2017)
Article
Plant Sciences
Irina Malinova, Saleh Alseekh, Regina Feil, Alisdair R. Fernie, Otto Baumann, Mark Aurel Schoettler, John E. Lunn, Joerg Fettke
Article
Plant Sciences
Irina Malinova, Harendra Mahto, Felix Brandt, Shadha AL-Rawi, Hadeel Qasim, Henrike Brust, Mahdi Hejazi, Joerg Fettke
Review
Biochemistry & Molecular Biology
Henrike Brust, Slawomir Orzechowski, Joerg Fettke
Article
Biochemistry & Molecular Biology
Slawomir Orzechowski, Dorota Sitnicka, Agnieszka Grabowska, Julia Compart, Joerg Fettke, Edyta Zdunek-Zastocka
Summary: Short-term cold treatment alters carbohydrate metabolism in potato leaves, leading to an increase in soluble sugar content.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Qingting Liu, Xiaoping Li, Joerg Fettke
Summary: By using laser confocal scanning microscopy, researchers found that in Arabidopsis mutants with altered starch metabolism, guard cells contained more but smaller plastidial starch granules compared to mesophyll cells. The morphological properties of the starch granules were indiscernible in both types of leaf cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Angel Merida, Joerg Fettke
Summary: This review discusses the initiation of starch granule formation and the controlling mechanism of granule numbers per plastid. It highlights the importance of polypeptides in providing support for starch granule formation and the strict regulation of granule numbers.
Article
Biochemistry & Molecular Biology
Qingting Liu, Yuan Zhou, Joerg Fettke
Summary: Transient starch plays a crucial role in plant metabolism. Monitoring alterations in starch granules provides valuable insights into the persistent metabolic process. This study demonstrates, for the first time, changes in starch granule size and morphology throughout the day and during leaf aging, as well as differences among starch-related mutants.
Article
Plant Sciences
Sidratul Nur Muntaha, Xiaoping Li, Julia Compart, Ardha Apriyanto, Joerg Fettke
Summary: The Arabidopsis double mutant lacking DPE2 and PHS1 shows a dwarf-growth phenotype, uneven starch distribution, and reduced starch granule number. In contrast, the triple mutant with an additional lack of GWD exhibits improved growth, starch-excess phenotype, and homogeneous starch distribution. Ongoing starch degradation is found to be the main cause of the observed double mutant phenotype.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Aakanksha Singh, Julia Compart, Shadha Abduljaleel AL-Rawi, Harendra Mahto, Abubakar Musa Ahmad, Joerg Fettke
Summary: The non-catalytic starch-binding proteins LESV and ESV1 play a crucial role in starch metabolism, affecting phosphorylation, hydrolysis and synthesis processes. They also induce stable alterations in the glucan structures at the surface of starch granules.
Article
Plant Sciences
Slawomir Orzechowski, Julia Compart, Edyta Zdunek-Zastocka, Joerg Fettke
Summary: Climate change can negatively impact starch granule properties due to both long-term and short-term temperature stresses, highlighting the importance of comprehensive research in this area.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Plant Sciences
Junio Flores-Castellanos, Joerg Fettke
Summary: This study found that potato tubers can immediately take up externally supplied glucose-1-phosphate and convert it into maltodextrins. The plastidial glucan phosphorylase is responsible for producing longer maltooligosaccharides in the presence of glucose-1-phosphate. These findings contribute to a better understanding of the role of the plastidial phosphorylase in starch metabolism.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Omonike O. Ogbole, Olufunke D. Akin-Ajani, Tolulope O. Ajala, Queeneth A. Ogunniyi, Joerg Fettke, Oluwatoyin A. Odeku
Summary: This article provides a comprehensive review of underutilized legumes in the sub-family Faboidea and highlights their untapped potential in terms of nutritional and pharmacological activities. The phytoconstituents from these plants could serve as lead compounds for drug discovery for various disorders.
Article
Biochemistry & Molecular Biology
Junio Flores Castellanos, Arsalan Khan, Joerg Fettke
Summary: Proteomic approaches have revealed the interaction between potato starch granules and various proteins, most of which are involved in starch metabolism. The study also found that GWD and PWD, two dikinases associated with starch degradation, play an important role in storage starch synthesis. Additionally, there were differences in the starch-interacting protein profiles of transgenic and wild type tubers under different storage temperatures, indicating differential expression in response to environmental changes.
Review
Polymer Science
Julia Compart, Aakanksha Singh, Joerg Fettke, Ardha Apriyanto
Summary: Starch is a convenient and economically important polymer in the food and processing industry. However, native starches have limited applications for industrial usage. Therefore, starch modification is carried out to enhance positive characteristics and eliminate limitations. This review summarizes possible starch modifications and their corresponding applications, both within and outside the plant system (physical, chemical, and enzymatic). Furthermore, it highlights the implications of adjusting different starch properties.